In sacco evaluation of ruminal degradability of isoflavones from full‐fat soybean and extracted soybean meal—A pilot study

Author(s):  
Ludmila Křížová ◽  
Zuzana Němcová ◽  
Kateřina Dadáková ◽  
Mária Chrenková
Author(s):  
M.A. Chaso ◽  
T. Manso ◽  
F.J. Giráldez ◽  
A.R. Mantecón

The provision of grain supplements to ruminants provide extra fermentable organic matter for the rumen microbial biomass, but often results in a lower whole efficient of ruminal digestion (Obaraet al., 1991; Weakleyet al., 1983). The present study was conducted to evaluate the effect of different basal diets on rumen degradability of several feedstuffs.Ruminal digestion of three feedstuffs - soybean meal (SBM), lucerne hay (LH) and barley straw (BS) - was determined byin saccomethod according a 2 × 3 factorial design defined by 2 basal diets - lucerne hay (H) or lucerne hay plus barley (40:60; HB) - and three churra ewes fitted with ruminal cannula.


Author(s):  
B.W.H.E Prasetiyono ◽  
A. Subrata ◽  
B.I.M. Tampoebolon ◽  
Surono ◽  
Widiyanto

2018 ◽  
Vol 22 (2) ◽  
pp. 55
Author(s):  
Susi Dwi Widyawati ◽  
Wara Pratitis Sabar Suprayogi

The esperiment was conducted to evaluate the effect of dietary addition of feed supplement on ruminal degradation of native grass with nylon bag technique or in sacco technique. The trial was a completely randomized design experiment of a 4 replicates on 2 rumen fistulateed cattles. Native grass was added with 4 kind of seed supplements as a growth promoting feed supplement (GPFS) with different feeds composition, they were consist of iso Nitrogenous and iso Carboneous. GPFS A consist of soybean meal and ketepeng leaf, GPFS B consist of soybean meal and lemuru fish oil, GPFS C consist of Leucaena leaf and ketepeng leaf and GPFS D consist of Leucaena leaf and lemuru fish oil. Four ration were incubated in the rumen for 0, 2, 4, 8, 12, 24, 32, 48, and 72 hours. Both dry matter (DM) and organic matter (OM) disappearance of native grass were not significantly affected by different feed supplement. Also, DM, OM, and crude fiber (CF) during 72 hours incubation were not affected by addition of feed supplements. Four kind of feed supplements had the similar potential to improve the ruminal environment and ruminal degradation


2020 ◽  
Vol 60 (3) ◽  
pp. 379
Author(s):  
Ghodrat Mohammadi ◽  
Mostafa Malecky ◽  
Jamal Seifdavati

The present study aimed at determining in sacco and in vitro the nutritive value of lentil screenings (LS), common vetch screenings (CVS) and bitter vetch screenings (BVS). For this purpose, three experiments (Exp.) were conducted. Ruminal degradation characteristics of the screenings were determined in Exp. 1 by the nylon bag technique using three ruminally fistulated rams. Gas production kinetics, and ruminal degradability and fermentation indices of the screenings were determined using 144 and 24-h incubations, respectively in Exp. 2. In Exp. 3, a basal growing ration for lambs (control) was supplemented by 175 or 350 g/kg of either LS, CVS or BVS (LS175, LS350, CVS175, CVS350, BVS175 and BVS350 respectively), and ruminal degradability and fermentation of the rations were evaluated in vitro. Soluble and potentially degradable fractions of protein were 0.321 and 0.719, 0.362 and 0.688, and 0.333 and 0.707, for LS, BVS and CVS respectively. Metabolisable energy was 11.1, 11.6 and 12.1 MJ/kg DM for LS, BVS and CVS respectively. The screenings had comparable gas production profiles, ruminal digestibility and total volatile fatty acid concentration. However, CVS and BVS had a higher propionate and a lower acetate:propionate ratio than LS (P < 0.05). Including BVS and LS in the mixed ration increased total volatile fatty acid at both inclusion levels, but only BVS enhanced propionate proportion and lowered the acetate:propionate ratio (P < 0.05). These results revealed that these screenings have a good nutritional value and can be used in ruminant diets without adversely affecting the rumen fermentation.


Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 169
Author(s):  
Carlos A. Pantoja-Don Juan ◽  
Gabriela Gómez-Verduzco ◽  
Claudia C. Márquez-Mota ◽  
Guillermo Téllez-Isaías ◽  
Young M. Kwon ◽  
...  

This study was conducted to evaluate the productive performance, intestinal health, and description of the cecum microbiota in broilers supplemented with β-mannanases (MNs) and bacteriophages (BPs). Six hundred one-day-old broilers were divided into four groups and fed one of the following diets: CON—corn–soybean meal + 10 ppm enramycin (ENR); MN: CON + 500 ppm MN; BP: CON + 500 ppm BP; MN + BP: BP + 500 ppm MN. The BP and MN factors showed similar performances to ENR. MN improved the concentration of IgA in the jejunum at 35 days of age. The morphometric index (IM) of the thymus increased by adding MN, while BP increased the liver and thymus IM. The histological analysis showed that BP and MN improved the intestinal morphology. MN + BP showed a tendency to decrease the abundance of Proteobacteria and increase the abundance of Bacteroidetes, indicating better microbiota function. In conclusion, our results demonstrate that the combination of MN + BP has potential in poultry nutrition; however, we highly recommend further experiments to confirm this hypothesis.


2013 ◽  
Vol 53 (4) ◽  
pp. 292 ◽  
Author(s):  
Zengting Song ◽  
Xiaofang Dong ◽  
Jianming Tong ◽  
Zhihong Wang

Waste vinegar residue (WVR) is a by-product of vinegar processing by solid-state fermentation. In this study, two experiments were conducted to determine chemical composition and ruminal degradability of WVR and whether WVR can be used as a feedstuff for ruminants. In Experiment 1, 14 WVR samples were incubated in the rumen of three Inner Mongolia White Cashmere Goats to determine the in sacco ruminal degradability parameters for DM, crude protein (CP) and neutral detergent fibre (NDF). The NDF, CP and ether extract (EE) contents of the WVR samples were 796.6, 125.2 and 93.3 g/kg, respectively. The greatest variation was observed for lactic acid, which ranged from 150.39 to 4391.12 mg/kg with an average of 1023.84 mg/kg. The average A, B and C fractions of DM for the 14 samples of WVR were 122, 350, and 528 g/kg, respectively. The effective degradability of DM, CP and NDF were 275, 550 and 211 g/kg, respectively. In Experiment 2, the in sacco degradation parameters for DM, CP and NDF of Chinese leymus, corn stalk and WVR were evaluated in a 3 × 3 Latin-square design. The rapidly degradable fraction of the DM and NDF for WVR was significantly (P < 0.01) greater than that of Chinese leymus and corn stalk. No difference in rumen-degraded DM, rumen-degraded CP and rumen-degraded NDF was found between Chinese leymus and WVR (P > 0.05). It is concluded that, WVR is not well digested ruminally but is a good source of protein for ruminants giving it value as an ingredient in ruminant diets.


1996 ◽  
Vol 76 (1) ◽  
pp. 89-94 ◽  
Author(s):  
J. W. G. Nicholson ◽  
R. E. McQueen ◽  
J. G. Allen ◽  
R. S. Bush

The nutritive value of dried shellfish waste (referred to as crab meal) produced in New Brunswick in the mid-1980s was evaluated by physical, chemical and biological assays. Typically the crab meal was high in ash content (about 50%), consisting mainly of calcium carbonate and contained about 20% chitin, 25% crude protein (CP) and less than 2% lipid material. The meal could be separated by screening into a coarse fraction high in ash and chitin and a fine fraction lower in ash and higher in CP. Although the reactivity rate of crab meal ash was not as high as that of similar sized limestone particles (42 vs. 30.4 min for particles that passed a 500-μm screen but were retained on a 300-μm screen), it was high enough to suggest crab meal could be a useful rumen buffer. The CP of crab meal was highly resistant to degradation in the rumen in sacco. Less than 18% of the crab meal CP disappeared from bags suspended in the rumen for 24 h, compared with more than 87% for soybean meal and full-fat canola seed. The digestibility of crab meal dry matter by sheep was only 34.3 ± 3.7%, but the CP digestibility was 69.6 ± 4.1%. The chemical and physical attributes assayed and the results of the in sacco and sheep digestibility trials all suggest that crab meal should be a useful supplement for diets low in digestible undergraded protein. However its inclusion in a supplement for beef calves fed legume silage resulted in lower feed consumption and rate of gain (P < 0.01). Feeding soybean meal or full-fat canola resulted in a significant increase (P < 0.01) in feed efficiency, compared with calves fed the control and crab meal supplements. It was concluded that crab meal produced in New Brunswick would be a useful supplement for beef cattle if problems of palatability could be overcome. Key words: Crab meal, rumen degradability, digestibility, protein, palatability


Author(s):  
Vladimír Majer ◽  
Pavel Veselý

The submitted thesis aims to assess the differences between the ruminal degradability and intestinal digestibility of crude protein contained in brewer’s grains (BG) and maize draff (AMG). The effectiveness of ruminal degradability was tested using the “in sacco” method on 3 dry Holstain cows fitted with rumen cannulas. The dynamics of ruminal degradability of crude protein (CP) was detected after 0, 4, 8, 16, and 24 hours of samples incubation in the rumen. The intestinal digestibility of crude protein undegradable in the rumen was determined using the “mobile bag” method on 3 dry Holstain cows fitted with duodenal cannulas. The crude protein degradability of BG was detected in the above-mentioned hours (%): 4.06; 18.16; 32.40; 38.56, and 50.70; crude protein degradability of AMG: 42.04; 63.56; 84.47; 85.16, and 87.19. The effectiveness of rumen degradability of BG crude protein at the rate of passage of rumen content 6 % per hour was calculated at 35.33 % and that of AMG, at 76.29 %. Intestinal digestibility of BG crude protein and dry matter at the rate of passage of intestinal content 6 % per hour was calculated at 79.41 % and 22.84 %, respectively, and that of AMG, at 57.01 % and 11.33 %, respectively. The differences between the indicators of both feedstuffs were significant (P < 0.05). The results show that BG are mostly a source of crude protein with higher intestinal digestibility than AMG.


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