scholarly journals DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

2020 ◽  
Vol 25 ◽  
pp. 03004
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Sergey Tokmakov ◽  
Victoriya Kotlyar
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Table Grape ◽  
Chemical Treatments ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Special Software ◽  
Grape Varieties

DNA markers are widely used in grapevine breeding to create forms with combined resistance genes. Downy mildew is one of the most common fungal diseases of the vine in the world. Growing grapevines with increased resistance allows to reduce the number of chemical treatments. The decrease in the use of pesticides is especially significant for viticulture of table varieties, since berries are directly consumed by humans for food. Currently, more than 20 resistance genes have been identified by molecular methods, and DNA markers for many genes have been developed. The genes Rpv3 (inherited from North American grape species) and Rpv12 (derived from V. amurensis) are among the most effective and have an additive effect. The study of 14 table grape varieties for the presence of the Rpv3 gene and 8 varieties for the presence of the Rpv12 gene was performed by using DNA-marker analysis. The analysis included varieties that could inherit these genes from the parent forms, according to their ancestry. The study was conducted using an automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner, DNA-markers were taken from literature sources. According to the results of DNA-marker analysis, 9 varieties were identified, including 2 seedless varieties, with the Rpv3299-279 allele in the genotypes, which determines resistance to downy mildew, and 3 table varieties with the Rpv12 gene in the genotypes. One table grape genotype was identified with Rpv3 and Rpv12.

scholarly journals Search for Rpv3 and Rpv12 genes in genotypes of table and seedless grape varieties using DNA-markers

2020 ◽  
Author(s):  
M. V. Makarkina ◽  
E. T. Ilnitskaya ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Seedless Grapes ◽  
Grape Varieties

DNA-marker analysis of grape genotypes for the presence of downy mildew resistance genes Rpv3 and Rpv12 was performed. Table and seedless grapes varieties and forms carring these resistance genes were identified according to the DNA-marker evaluation.

scholarly journals Search for donors of powdery mildew resistance genes among seedless and table grape varieties

2021 ◽  
Vol 39 ◽  
pp. 02005
Author(s):  
Marina Makarkina ◽  
Elena Ilnitskaya ◽  
Tatiana Kozina
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Table Grape ◽  
Main Method ◽  
Chemical Treatments ◽  
Pesticide Treatment ◽  
Resistant Varieties ◽  
Allelic Status ◽  
Grape Varieties

Powdery mildew (Erysiphe necator) is one of the most common and economically significant diseases of grapes. Currently, the main method of controlling the disease is pesticide treatment. Breeding of resistant varieties is necessary to reduce chemical treatments. Currently, a number of grape resistance genes to powdery mildew and DNA markers for identification the allelic status of these genes are known. In a study to determine the presence of resistance loci Ren3 and Ren9, 25 genotypes of table grape varieties were analyzed, including 18 seedless varieties. DNA markers GF15-42, ScORGF15-02 were used to identify Ren3 gene, and CenGen6 – to identify Ren9 gene. DNA of cultivars Regent and Seyve Villard 12-375, which have resistance alleles, were used as positive controls. As a result of DNA marker analysis, it was determined that genotypes of table varieties Viking, Kodryanka, Moldova, Nadezhda AZOS, Original and seedless varieties Pamyati Smirnova, Kishmish Zaporozhskiy and Kishmish 342 carry loci of resistance to powdery mildew Ren3 and Ren9.

scholarly journals DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

2020 ◽  
Vol 2 (3) ◽  
pp. 15-19
Author(s):  
E. T. Ilnitskaya ◽  
M. V. Makarkina ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Gene ◽  
Resistance Genes ◽  
North American ◽  
Dna Marker ◽  
North American Species ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Seedless Grape ◽  
Grape Varieties

Table grapes are a valuable dietary product. Seedless grapes are in high demand among consumers. For this reason, the breeding of seedless varieties is one of the popular trends in modern viticulture, along with the production of environmentally friendly products. Downy mildew (Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni) is one of the most common fungal diseases of the grapevine. Most downy mildew resistant grape accessions belong to North American species like Vitis aestivalis Michx., V. berlandieri Planch., V. cinerea (Engelm. ex A. Gray) Engelm. ex Millard, V. riparia Michx., V. rupestris  Scheele, etc. The search for donors of resistance genes is an urgent task. Rpv3 is one of the most significant resistance genes from a number of North American grape varieties. The aim of this work is to identify the downy mildew resistance gene Rpv3 in seedless grape varieties by means of DNA-marker analysis. The grape varieties with rudimentary development of seed in berries and with North American species in the pedigree were chosen as the object of the study. The varieties “Dunavski lazur” and “Seyve Villard 12-375” with reference alleles were used as the positive control, while V. vinifera L. was used as the negative control. UDV305 and UDV737 DNA-markers were used in this study to identify the allelic type of the Rpv3 gene. The work was performed using the polymerase chain reaction. The reaction products were separated by capillary electrophoresis using the ABI Prism 3130 automatic genetic analyzer. Evaluation of the results was done using the GeneMapper and PeakScanner software. Functional alleles of the downy mildew resistance gene Rpv3 were revealed in grape  varieties “Kishmish zaporozhskiy”, “Lady Patricia”, “Remaily seedless”, “Pamyati Smirnova” and “Shayan”. Rpv3299-279, one of the seven known haplotypes, was identified in all the varieties. The pedigree analysis of the studied varieties indicated that the parental forms – “Seyve Villard” and “Seibel” hybrids – are presumably the donors of the gene. Grape accessions with the identified Rpv3 gene can be used in seedless varieties breeding as donors of resistance to downy mildew.

scholarly journals Potential of genetic resistance of new table grape hybrids to fungal pathogens

2021 ◽  
Vol 34 ◽  
pp. 02001
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Valeriy Petrov
Keyword(s):  
Powdery Mildew ◽  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Fungal Pathogens ◽  
Genetic Resistance ◽  
Plasmopara Viticola ◽  
Table Grape ◽  
Table Grapes ◽  
Hybrid Forms

Downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) are the most common and economically significant fungal diseases in vineyards. The task of this work is to study the genotypes of new promising hybrid forms of table grapes for the presence of resistance genes to downy mildew (Rpv10 and Rpv3) and powdery mildew (Ren9) using DNA-markers. The study was carried out on table grape hybrids under the working names Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya and registered variety Liviya. The studied genes were analyzed using markers UDV305 and UDV737 (Rpv3), GF09-46 (Rpv10), CenGen6 (Ren9). The following cultivars were used as reference genotypes: Saperavi severnyi (carries Rpv10 gene) and Regent (Rpv3 and Ren9). It was established that Rpv3 gene is carried by hybrids Kishmish dubovskiy, Agat dubovskiy, Kurazh, Valensiya, Akelo, Gamlet, Dubovskiy rozovyi, Pestryi. Ren9 gene was found in Artek, Agat dubovskiy, Kurazh, Ispolin, Valensiya, Arabella, Gamlet, Dubovskiy rozovyi, Pestryi. The Rpv10 gene was not detected in any of the analyzed grapevine samples. genotypes Agat dubovskiy, Kurazh, Gamlet, Dubovskiy rozovyi, Pestryi, Valensiya carry Rpv3 and Ren9 genes simultaneously. These grapevines have an elegant bunch and large berries that are attractive to consumers.

scholarly journals Temporal dynamics and management of downy mildew on the table grape ‘BRS Vitória’ in northern Paraná

2018 ◽  
Vol 39 (1) ◽  
pp. 19
Author(s):  
Carolina Bertuzzi Pereira ◽  
Dauri José Tessmann ◽  
Rosangela Getirana Santana ◽  
Rodrigo Iván Contreras-Soto ◽  
Reginaldo Teodoro de Souza ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Disease Severity ◽  
Fruit Ripening ◽  
Temporal Dynamics ◽  
Severity Index ◽  
Table Grape ◽  
Repeated Measurements ◽  
Related Factors ◽  
Spring Crop ◽  
Seedless Grape

Downy mildew caused by Plasmopara viticola is a major disease of grapes in Paraná State, Brazil and other wine-producing regions. The seedless table grape ‘BRS Vitória’ stands out for its tolerance to this disease and is a viable alternative to reduce fungicide applications. The objectives of this study were to analyze the influence of weather-related factors, such as temperature and rainfall on disease progress and to evaluate the efficacy of fungicide spray programs for controlling downy mildew of grapes during the summer-fall and winter-spring crop seasons in northern Paraná, Brazil. Field trials were conducted in Marialva, Paraná, during the winter-spring crop seasons (August to December) in 2013 and 2014 and the summer-fall crop seasons (January to May) in 2014 and 2015. The experimental design used was completely randomized with repeated measurements over the phenological periods, with five treatments and 10 replicates. The following treatments were compared: (1) conventional, in which fungicides were applied according to conventional standards; (2) preventive fungicide sprays every seven days; (3) preventive fungicide sprays every 14 days; (4) fungicide sprays after observing the first downy mildew symptoms; and (5) no fungicide sprays. In the conventional fungicide program, sprays were performed two or three times per week. Treatments 2 and 3 received sprayings between the start of branch budding and fruit ripening. In treatment 4, sprays started from the first occurrence of oil spot symptoms, and then, the applications were spaced every seven days until fruit ripening. The severity of disease was evaluated weekly. The disease was severe only in the 2014 and 2015 summer-fall crop seasons, reaching a maximum severity of 17.3% and 21.3% of the leaf area, respectively. The highest disease severity in the summer-fall crop seasons was associated with higher frequencies of rainy days and higher temperatures. Disease severity in conventional treatments did not differ from severity in treatments with sprayings made every seven and 14 days during the summer-fall crops in both years. Severity index on bunches were not observed for the evaluated trials. The seedless grape ‘BRS Vitória’ is less dependent on the use of fungicides to control downy mildew in northern Paraná.

scholarly journals DNA-marker based identification of the RPV3 gene determining downy mildew resistance in grapevines

2018 ◽  
Vol 22 (6) ◽  
pp. 703-707 ◽  
Author(s):  
E. T. Ilnitskaya ◽  
M. V. Makarkina ◽  
S. V. Tokmakov ◽  
L. G. Naumova
Keyword(s):  
Downy Mildew ◽  
Dna Marker ◽  
Genetic Resistance ◽  
Plasmopara Viticola ◽  
Resistant Varieties ◽  
Allelic Status ◽  
Polymerase Chain ◽  
Grape Varieties ◽  
Genetic Analyzer ◽  
Selection Of

Downy mildew is one of the most common fungal diseases of the vine, caused by Plasmopara viticola. An effective way to control the spread of the pathogen is to cultivate resistant varieties. Cultivars of Vitis vinifera, being the basis of high-quality viticulture, practically do not possess genetic resistance to P. viticola, so screening for resistance donors is an important stage in breeding. One of the major resistance loci to downy mildew, the Rpv3 gene, was identified in the genotype of a complex interspecific hybrid of grapes Bianca. Later, it was found that this gene had seven haplotypes of resistance inherited from North American grape species, and that it was possible to identify the allelic status of the gene using DNA-markers UDV305, UDV737. However, only two haplotypes can be combined in one diploid form. To determine the Rpv3 gene in the grape gene pool we, using these markers, studied 35 different genotypes of grapevines, most of which are interspecies cultivars. Three varieties with known allelic status of the Rpv3 gene (Dunavski lazur, Noah, Seyve Villard 12-375) were included in the study as reference genotypes. The genotypes were studied through polymerase chain reaction with separation of amplification products by capillary electrophoresis in automatic genetic analyzer ABI Prism 3130. In the studied grape cultivars DNA marker analysis indentified the Rpv3 gene in sixteen genotypes of interspecific origin, including haplotype Rpv3299-279 found in twelve varieties, Rpv3321-312 – in three, and haplotype Rpv3null-271 – in one variety. Seyve Villard 12-375 turned out to be the donor of resistance gene in the most of the genotypes carrying Rpv3 in this study. The obtained data can be useful in selection of mildew resistant grape varieties and screening for hybridization pairs.

Quelques résultats de la sélection menée en République populaire de Bulgarie en vue de l'obtention de cépages de table et de cuve précoces

OENO One ◽  
1977 ◽  
Vol 11 (4) ◽  
pp. 277
Author(s):  
K. Stoev
Keyword(s):  
Table Grape ◽  
Wine Grape ◽  
Seedless Grape ◽  
Grape Varieties

<p style="text-align: justify;">La sélection réalisée en Bulgarie depuis 1951 a permis d'obtenir des cépages de cuve, des cépages de table et des cépages apyrènes présentant des caractéristiques intéressantes, notamment en ce qui concerne la précocité et la résistance au froid. L'auteur donne les caractéristiques de 11 de ces cépages (morphologie des grappes, phénologie, aptitudes).</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">The selection carried out in Bulgaria since 1951 has permitted wine grape varieties, table grape-varieties and apyren (seedless) grape varieties to be obtained which present interesting characteristics, especially with degard to precocity and resistance to cold. The writer gives the characteristics of 11 of these grape varieties (morphology of the bunches, phenology, aptitudes).</p>

Sign in / Sign up

Export Citation Format

Share Document