scholarly journals DNA-marker based identification of the RPV3 gene determining downy mildew resistance in grapevines

2018 ◽  
Vol 22 (6) ◽  
pp. 703-707 ◽  
Author(s):  
E. T. Ilnitskaya ◽  
M. V. Makarkina ◽  
S. V. Tokmakov ◽  
L. G. Naumova
Keyword(s):  
Downy Mildew ◽  
Dna Marker ◽  
Genetic Resistance ◽  
Plasmopara Viticola ◽  
Resistant Varieties ◽  
Allelic Status ◽  
Polymerase Chain ◽  
Grape Varieties ◽  
Genetic Analyzer ◽  
Selection Of

Downy mildew is one of the most common fungal diseases of the vine, caused by Plasmopara viticola. An effective way to control the spread of the pathogen is to cultivate resistant varieties. Cultivars of Vitis vinifera, being the basis of high-quality viticulture, practically do not possess genetic resistance to P. viticola, so screening for resistance donors is an important stage in breeding. One of the major resistance loci to downy mildew, the Rpv3 gene, was identified in the genotype of a complex interspecific hybrid of grapes Bianca. Later, it was found that this gene had seven haplotypes of resistance inherited from North American grape species, and that it was possible to identify the allelic status of the gene using DNA-markers UDV305, UDV737. However, only two haplotypes can be combined in one diploid form. To determine the Rpv3 gene in the grape gene pool we, using these markers, studied 35 different genotypes of grapevines, most of which are interspecies cultivars. Three varieties with known allelic status of the Rpv3 gene (Dunavski lazur, Noah, Seyve Villard 12-375) were included in the study as reference genotypes. The genotypes were studied through polymerase chain reaction with separation of amplification products by capillary electrophoresis in automatic genetic analyzer ABI Prism 3130. In the studied grape cultivars DNA marker analysis indentified the Rpv3 gene in sixteen genotypes of interspecific origin, including haplotype Rpv3299-279 found in twelve varieties, Rpv3321-312 – in three, and haplotype Rpv3null-271 – in one variety. Seyve Villard 12-375 turned out to be the donor of resistance gene in the most of the genotypes carrying Rpv3 in this study. The obtained data can be useful in selection of mildew resistant grape varieties and screening for hybridization pairs.

Downy mildew is able to carry out its sexual cycle on resistant grape varieties

2020 ◽  
Author(s):  
Lionel Delbac ◽  
Laurent Delière ◽  
Christophe Schneider ◽  
François Delmotte
Keyword(s):  
Life Cycle ◽  
Downy Mildew ◽  
Host Resistance ◽  
Genetic Resistance ◽  
Sexual Cycle ◽  
Resistant Varieties ◽  
One Year ◽  
Pathogen Populations ◽  
Grape Varieties ◽  
Grape Cultivars

Previous studies have shown that new resistant grape cultivars limit epidemics caused by asexual reproduction of downy mildew. However, until now, there was a lack of knowledge on the sexual phase of the pathogen. This study demonstrates that host resistance has little effect on the sexual cycle of downy mildew. Thus, the pathogen can complete its life cycle (asexual and sexual phases) on partially resistant grapevine varieties. The persistence of pathogen populations on partially resistant varieties from one year to the next represents a major challenge for the sustainability of genetic resistance of grapevines.

scholarly journals A study of the genetic polymorphism of Plasmopara viticola in the vineyards of the Krasnodar Territory

2020 ◽  
Vol 23 ◽  
pp. 02005
Author(s):  
Marina V. Makarkina ◽  
Sergey V. Tokmakov ◽  
Elena T. Ilnitskaya
Keyword(s):  
Genetic Diversity ◽  
Downy Mildew ◽  
Prediction Models ◽  
Dna Marker ◽  
Plasmopara Viticola ◽  
Fragment Analysis ◽  
Grapevine Downy Mildew ◽  
Grape Varieties ◽  
First Time ◽  
Different Levels

Plasmopara viticola oomycete is a seasonal pathogen that causes one of the most harmful diseases of the grapevine – downy mildew. The study of the biodiversity of Plasmopara viticola in various zones of viticulture has fundamental goals, as well as practical ones, as it is important for understanding the epidemiological cycle of P. viticola and for refining disease prediction models. The purpose of the work is to study the genetic diversity of P. viticola in the vineyards of the Krasnodar Territory, including on the grape varieties with different levels of resistance to downy mildew. The study was conducted on pathogens samples collected on grape plants of varieties with varying degrees of resistance to downy mildew. The collection of material was carried out in May-August 2019 in various zones of the Krasnodar Territory. 48 samples of P. viticola were analyzed. The DNA markers BER, ISA, CES, GOB, PV91, PV137, PV143, PV144 recommended for studying the genetic diversity of P. viticola were used. The work was performed by PCR. The amplification products were evaluated by the method of fragment analysis. DNA-marker GOB identified 37 alleles of different sizes, PV144 – 20, CES – 10, BER – 3, PV91 – 3, PV137 – 2, ISA – 1, PV143 – 1. It was shown that P. viticola populations are variable on different varieties and in different geographical areas. This study was conducted in the Krasnodar Territory for the first time.

scholarly journals The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen Plasmopara viticola

2021 ◽  
Vol 22 (2) ◽  
pp. 940
Author(s):  
Elodie Vandelle ◽  
Pietro Ariani ◽  
Alice Regaiolo ◽  
Davide Danzi ◽  
Arianna Lovato ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Ubiquitin Ligase ◽  
Control Measure ◽  
Durable Resistance ◽  
Genetic Resistance ◽  
E3 Ubiquitin Ligase ◽  
Plasmopara Viticola ◽  
Vitis Vinifera L ◽  
Vitis Species ◽  
Vitis Riparia

Downy mildew, caused by Plasmopara viticola, is one of the most severe diseases of grapevine (Vitis vinifera L.). Genetic resistance is an effective and sustainable control strategy, but major resistance genes (encoding receptors for specific pathogen effectors) introgressed from wild Vitis species, although effective, may be non-durable because the pathogen can evolve to avoid specific recognition. Previous transcriptomic studies in the resistant species Vitis riparia highlighted the activation of signal transduction components during infection. The transfer of such components to V. vinifera might confer less specific and therefore more durable resistance. Here, we describe the generation of transgenic V. vinifera lines constitutively expressing the V. riparia E3 ubiquitin ligase gene VriATL156. Phenotypic and molecular analysis revealed that the transgenic plants were less susceptible to P. viticola than vector-only controls, confirming the role of this E3 ubiquitin ligase in the innate immune response. Two independent transgenic lines were selected for detailed analysis of the resistance phenotype by RNA-Seq and microscopy, revealing the profound reprogramming of transcription to achieve resistance that operates from the earliest stages of pathogen infection. The introduction of VriATL156 into elite grapevine cultivars could therefore provide an effective and sustainable control measure against downy mildew.

scholarly journals Potential of genetic resistance of new table grape hybrids to fungal pathogens

2021 ◽  
Vol 34 ◽  
pp. 02001
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Valeriy Petrov
Keyword(s):  
Powdery Mildew ◽  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Fungal Pathogens ◽  
Genetic Resistance ◽  
Plasmopara Viticola ◽  
Table Grape ◽  
Table Grapes ◽  
Hybrid Forms

Downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) are the most common and economically significant fungal diseases in vineyards. The task of this work is to study the genotypes of new promising hybrid forms of table grapes for the presence of resistance genes to downy mildew (Rpv10 and Rpv3) and powdery mildew (Ren9) using DNA-markers. The study was carried out on table grape hybrids under the working names Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya and registered variety Liviya. The studied genes were analyzed using markers UDV305 and UDV737 (Rpv3), GF09-46 (Rpv10), CenGen6 (Ren9). The following cultivars were used as reference genotypes: Saperavi severnyi (carries Rpv10 gene) and Regent (Rpv3 and Ren9). It was established that Rpv3 gene is carried by hybrids Kishmish dubovskiy, Agat dubovskiy, Kurazh, Valensiya, Akelo, Gamlet, Dubovskiy rozovyi, Pestryi. Ren9 gene was found in Artek, Agat dubovskiy, Kurazh, Ispolin, Valensiya, Arabella, Gamlet, Dubovskiy rozovyi, Pestryi. The Rpv10 gene was not detected in any of the analyzed grapevine samples. genotypes Agat dubovskiy, Kurazh, Gamlet, Dubovskiy rozovyi, Pestryi, Valensiya carry Rpv3 and Ren9 genes simultaneously. These grapevines have an elegant bunch and large berries that are attractive to consumers.

scholarly journals Search for donors of powdery mildew resistance genes among seedless and table grape varieties

2021 ◽  
Vol 39 ◽  
pp. 02005
Author(s):  
Marina Makarkina ◽  
Elena Ilnitskaya ◽  
Tatiana Kozina
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Table Grape ◽  
Main Method ◽  
Chemical Treatments ◽  
Pesticide Treatment ◽  
Resistant Varieties ◽  
Allelic Status ◽  
Grape Varieties

Powdery mildew (Erysiphe necator) is one of the most common and economically significant diseases of grapes. Currently, the main method of controlling the disease is pesticide treatment. Breeding of resistant varieties is necessary to reduce chemical treatments. Currently, a number of grape resistance genes to powdery mildew and DNA markers for identification the allelic status of these genes are known. In a study to determine the presence of resistance loci Ren3 and Ren9, 25 genotypes of table grape varieties were analyzed, including 18 seedless varieties. DNA markers GF15-42, ScORGF15-02 were used to identify Ren3 gene, and CenGen6 – to identify Ren9 gene. DNA of cultivars Regent and Seyve Villard 12-375, which have resistance alleles, were used as positive controls. As a result of DNA marker analysis, it was determined that genotypes of table varieties Viking, Kodryanka, Moldova, Nadezhda AZOS, Original and seedless varieties Pamyati Smirnova, Kishmish Zaporozhskiy and Kishmish 342 carry loci of resistance to powdery mildew Ren3 and Ren9.

scholarly journals Testing of paraffin oil efficiency against grape powdery mildew in Eger wine region

2016 ◽  
pp. 73-80
Author(s):  
Xénia Pálfi ◽  
Dénes Bisztray ◽  
Szabolcs Villangó ◽  
Zita Pálfi ◽  
Tamás Deák ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Downy Mildew ◽  
Plasmopara Viticola ◽  
Field Trials ◽  
Intensity Of Infection ◽  
Erysiphe Necator ◽  
Regular Treatment ◽  
Paraffin Oil ◽  
Wine Region ◽  
Grape Varieties

The aim of the present study was to examine the efficiency of paraffin oil against powdery mildew in Eger wine region. The experiment has been carried out in 2013 and 2014 with Chardonnay and Kékfrankos grape varieties, which have different resistance against powdery mildew. The effectiveness of the oil was examined on leaves and clusters (frequency and intensity). This oil was effective against Erysiphe necator infection on field trials in Chile and Brazil. The spread of downy mildew (Plasmopara viticola) was also inhibited by this material in some experiments conducted in Spain and France. The differences between oil treatments represented the sensitivity of the grape varieties in accordance with the applied dosages. The oil was effective against powdery mildew with different extent as a result of the so called ”vintage effect”. In 2013, the treatment of the highest dosage (D3) didn’t differed significantly in frequency and intensity of infection from the regular treatment (clusters of Kékfrankos, leaves of Chardonnay). In 2014, the oil was not so effective against powdery mildew compared to 2013. No remarkable differences were detected between the treatments due to the strong pressure of powdery mildew. Furthermore, no any effect of the lowest dosage (D1) was detected in the case of the sensitive clusters of Chardonnay and leaves of Kékfrankos in both experimental years. In summary, the oil treatment has an effect against powdery mildew, however this efficiency largely depends on the vintage characteristics and the pressure of powdery mildew. Further investigations are neccessary, for example field trials with combinations of other sprays. The oil can be useable as fungicide with proper care in eco-friendly integrated and bio (ecological) viticulture.

scholarly journals Search for Rpv3 and Rpv12 genes in genotypes of table and seedless grape varieties using DNA-markers

2020 ◽  
Author(s):  
M. V. Makarkina ◽  
E. T. Ilnitskaya ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Seedless Grapes ◽  
Grape Varieties

DNA-marker analysis of grape genotypes for the presence of downy mildew resistance genes Rpv3 and Rpv12 was performed. Table and seedless grapes varieties and forms carring these resistance genes were identified according to the DNA-marker evaluation.

DNA Marker-Based High-Throughput Identification of Downy Mildew Infected and Non-Infected Sunflower Plants

Helia ◽  
2019 ◽  
Vol 42 (70) ◽  
pp. 37-43
Author(s):  
Anzhella Solodenko
Keyword(s):  
Downy Mildew ◽  
High Throughput ◽  
Unmet Needs ◽  
Dna Marker ◽  
Independent Set ◽  
The Past ◽  
Seedling Inoculation ◽  
Causal Pathogen ◽  
Total Dna ◽  
Selection Of

AbstractDeveloping hybrids, resistant to causal pathogen of Downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni), is one of the critical tasks in sunflower breeding. Molecular markers have advanced breeding practice in the past decades, however there are still unmet needs for reliable high-throughput (HT) selection of the pathogen resistant starting material and differentiation of the plants infected by different pathogens. In this study, we tested the known DNA marker (308 bp fragment from ribosomal DNA of P. halstedii) for detection of pathogen in different tissues of sunflower plant and at different stages of plant development. Specified DNA marker was re-validated in the total DNA, isolated from sporangium as well as from seedlings of infected pathogen resistant/susceptible inbred lines of Ukrainian breeding and 60 F2 crosses. An independent set of field grown plants with unknown resistance to P. halstedii, having symptoms of the bacterial/fungal/viral pathology were used for HT screening and genotypes, infected with downy mildew, were successfully identified. Pathogen appeared to be concentrated in the vessels of sunflower leaves, in contrast to parenchymal tissue. Our study demonstrates an addition to whole seedling inoculation technique of P. halstedii detection, which allows HT identification of the pathogen infected and non-infected sunflower plants.

scholarly journals Rpv Mediated Defense Responses in Grapevine Offspring Resistant to Plasmopara viticola

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 781
Author(s):  
Tyrone Possamai ◽  
Daniele Migliaro ◽  
Massimo Gardiman ◽  
Riccardo Velasco ◽  
Barbara De Nardi
Keyword(s):  
Downy Mildew ◽  
Cost Effective ◽  
Plasmopara Viticola ◽  
Defense Responses ◽  
Resistance Level ◽  
Resistance Response ◽  
Host Pathogen Interaction ◽  
Resistant Varieties ◽  
New Varieties ◽  
Independent Features

Downy mildew, caused by the biotrophic oomycete Plasmopara viticola, is one of the most serious grapevine diseases. The development of new varieties, showing partial resistance to downy mildew, through traditional breeding provides a sustainable and effective solution for disease management. Marker-assisted-selection (MAS) provide fast and cost-effective genotyping methods, but phenotyping remains necessary to characterize the host–pathogen interaction and assess the effective resistance level of new varieties as well as to validate MAS selection. In this study, the Rpv mediated defense responses were investigated in 31 genotypes, encompassing susceptible and resistant varieties and 26 seedlings, following inoculation of leaf discs with P. viticola. The offspring differed in Rpv loci inherited (none, one or two): Rpv3-3 and Rpv10 from Solaris and Rpv3-1 and Rpv12 from Kozma 20-3. To improve the assessment of different resistance responses, pathogen reaction (sporulation) and host reaction (necrosis) were scored separately as independent features. They were differently expressed depending on Rpv locus: offspring carrying Rpv3-1 and Rpv12 loci showed the strongest resistance response (scarce sporulation and necrosis), those carrying Rpv3-3 locus showed the highest levels of necrosis while Rpv10 carrying genotypes showed intermediate levels of both sporulation and necrosis.

scholarly journals DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

2020 ◽  
Vol 25 ◽  
pp. 03004
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Sergey Tokmakov ◽  
Victoriya Kotlyar
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Table Grape ◽  
Chemical Treatments ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Special Software ◽  
Grape Varieties

DNA markers are widely used in grapevine breeding to create forms with combined resistance genes. Downy mildew is one of the most common fungal diseases of the vine in the world. Growing grapevines with increased resistance allows to reduce the number of chemical treatments. The decrease in the use of pesticides is especially significant for viticulture of table varieties, since berries are directly consumed by humans for food. Currently, more than 20 resistance genes have been identified by molecular methods, and DNA markers for many genes have been developed. The genes Rpv3 (inherited from North American grape species) and Rpv12 (derived from V. amurensis) are among the most effective and have an additive effect. The study of 14 table grape varieties for the presence of the Rpv3 gene and 8 varieties for the presence of the Rpv12 gene was performed by using DNA-marker analysis. The analysis included varieties that could inherit these genes from the parent forms, according to their ancestry. The study was conducted using an automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner, DNA-markers were taken from literature sources. According to the results of DNA-marker analysis, 9 varieties were identified, including 2 seedless varieties, with the Rpv3299-279 allele in the genotypes, which determines resistance to downy mildew, and 3 table varieties with the Rpv12 gene in the genotypes. One table grape genotype was identified with Rpv3 and Rpv12.

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