Search for donors of powdery mildew resistance genes among seedless and table grape varieties

Powdery mildew (Erysiphe necator) is one of the most common and economically significant diseases of grapes. Currently, the main method of controlling the disease is pesticide treatment. Breeding of resistant varieties is necessary to reduce chemical treatments. Currently, a number of grape resistance genes to powdery mildew and DNA markers for identification the allelic status of these genes are known. In a study to determine the presence of resistance loci Ren3 and Ren9, 25 genotypes of table grape varieties were analyzed, including 18 seedless varieties. DNA markers GF15-42, ScORGF15-02 were used to identify Ren3 gene, and CenGen6 – to identify Ren9 gene. DNA of cultivars Regent and Seyve Villard 12-375, which have resistance alleles, were used as positive controls. As a result of DNA marker analysis, it was determined that genotypes of table varieties Viking, Kodryanka, Moldova, Nadezhda AZOS, Original and seedless varieties Pamyati Smirnova, Kishmish Zaporozhskiy and Kishmish 342 carry loci of resistance to powdery mildew Ren3 and Ren9.

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DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

BIO Web of Conferences ◽

10.1051/bioconf/20202503004 ◽

2020 ◽

Vol 25 ◽

pp. 03004

Author(s):

Elena Ilnitskaya ◽

Marina Makarkina ◽

Sergey Tokmakov ◽

Victoriya Kotlyar

Keyword(s):

Downy Mildew ◽

Resistance Genes ◽

Dna Markers ◽

Dna Marker ◽

Table Grape ◽

Chemical Treatments ◽

Marker Analysis ◽

Seedless Grape ◽

Special Software ◽

Grape Varieties

DNA markers are widely used in grapevine breeding to create forms with combined resistance genes. Downy mildew is one of the most common fungal diseases of the vine in the world. Growing grapevines with increased resistance allows to reduce the number of chemical treatments. The decrease in the use of pesticides is especially significant for viticulture of table varieties, since berries are directly consumed by humans for food. Currently, more than 20 resistance genes have been identified by molecular methods, and DNA markers for many genes have been developed. The genes Rpv3 (inherited from North American grape species) and Rpv12 (derived from V. amurensis) are among the most effective and have an additive effect. The study of 14 table grape varieties for the presence of the Rpv3 gene and 8 varieties for the presence of the Rpv12 gene was performed by using DNA-marker analysis. The analysis included varieties that could inherit these genes from the parent forms, according to their ancestry. The study was conducted using an automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner, DNA-markers were taken from literature sources. According to the results of DNA-marker analysis, 9 varieties were identified, including 2 seedless varieties, with the Rpv3299-279 allele in the genotypes, which determines resistance to downy mildew, and 3 table varieties with the Rpv12 gene in the genotypes. One table grape genotype was identified with Rpv3 and Rpv12.

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Potential of genetic resistance of new table grape hybrids to fungal pathogens

BIO Web of Conferences ◽

10.1051/bioconf/20213402001 ◽

2021 ◽

Vol 34 ◽

pp. 02001

Author(s):

Elena Ilnitskaya ◽

Marina Makarkina ◽

Valeriy Petrov

Keyword(s):

Powdery Mildew ◽

Downy Mildew ◽

Resistance Genes ◽

Dna Markers ◽

Fungal Pathogens ◽

Genetic Resistance ◽

Plasmopara Viticola ◽

Table Grape ◽

Table Grapes ◽

Hybrid Forms

Downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) are the most common and economically significant fungal diseases in vineyards. The task of this work is to study the genotypes of new promising hybrid forms of table grapes for the presence of resistance genes to downy mildew (Rpv10 and Rpv3) and powdery mildew (Ren9) using DNA-markers. The study was carried out on table grape hybrids under the working names Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya and registered variety Liviya. The studied genes were analyzed using markers UDV305 and UDV737 (Rpv3), GF09-46 (Rpv10), CenGen6 (Ren9). The following cultivars were used as reference genotypes: Saperavi severnyi (carries Rpv10 gene) and Regent (Rpv3 and Ren9). It was established that Rpv3 gene is carried by hybrids Kishmish dubovskiy, Agat dubovskiy, Kurazh, Valensiya, Akelo, Gamlet, Dubovskiy rozovyi, Pestryi. Ren9 gene was found in Artek, Agat dubovskiy, Kurazh, Ispolin, Valensiya, Arabella, Gamlet, Dubovskiy rozovyi, Pestryi. The Rpv10 gene was not detected in any of the analyzed grapevine samples. genotypes Agat dubovskiy, Kurazh, Gamlet, Dubovskiy rozovyi, Pestryi, Valensiya carry Rpv3 and Ren9 genes simultaneously. These grapevines have an elegant bunch and large berries that are attractive to consumers.

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Evaluation of Different Grape Varieties for Resistance to Powdery Mildew Caused by Uncinula Necator

Journal of Plant Protection Research ◽

10.2478/v10045-009-0069-2 ◽

2009 ◽

Vol 49 (4) ◽

pp. 434-439 ◽

Cited By ~ 2

Author(s):

H. Khiavi ◽

Haji Shikhlinski ◽

A. Ahari ◽

Asgar Heydari

Keyword(s):

Powdery Mildew ◽

Control Method ◽

Natural Infection ◽

Uncinula Necator ◽

International Programs ◽

Common Control ◽

Resistant Varieties ◽

Environmentally Sound ◽

Grape Varieties ◽

Chemical Pesticides

Evaluation of Different Grape Varieties for Resistance to Powdery Mildew Caused byUncinula NecatorPowdery mildew is one of the most serious and destructive diseases of grapes around the world including Iran. Although the application of chemical pesticides is the most common control method, the use of resistant varieties may be the most effective and environmentally sound strategy for managing the disease. Fourteen grape varieties were evaluated for testing powdery mildew (Uncinula necator) resistance under natural infection conditions and artificial inoculation methods were used during 2007-2008 in Ardabil province of Iran. Disease severity on leaves and fruit (cluster) were evaluated using Voytovich (1987) procedure. Results of the experiments showed that Shahani, Yagothi and Tabrizkishmishi cultivars were immune, Sahibi cultivar was highly resistant, Agshilig and Tokoulgan were resistant, Kishmishi, Khalili and Tabarzeh cultivars were tolerant, Aldarag and Koupakbogan were susceptible and Garashilig, Seyrakpousteh and Rasmi cultivars were highly susceptible to the disease. In general, results of this study were promising and some immune, highly resistant and resistant cultivars toU. necatorwere identified and they may be used as a resistance genetic source for management of the disease in national and international programs.

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DNA-marker based identification of the RPV3 gene determining downy mildew resistance in grapevines

Vavilov Journal of Genetics and Breeding ◽

10.18699/vj18.413 ◽

2018 ◽

Vol 22 (6) ◽

pp. 703-707 ◽

Cited By ~ 2

Author(s):

E. T. Ilnitskaya ◽

M. V. Makarkina ◽

S. V. Tokmakov ◽

L. G. Naumova

Keyword(s):

Downy Mildew ◽

Dna Marker ◽

Genetic Resistance ◽

Plasmopara Viticola ◽

Resistant Varieties ◽

Allelic Status ◽

Polymerase Chain ◽

Grape Varieties ◽

Genetic Analyzer ◽

Selection Of

Downy mildew is one of the most common fungal diseases of the vine, caused by Plasmopara viticola. An effective way to control the spread of the pathogen is to cultivate resistant varieties. Cultivars of Vitis vinifera, being the basis of high-quality viticulture, practically do not possess genetic resistance to P. viticola, so screening for resistance donors is an important stage in breeding. One of the major resistance loci to downy mildew, the Rpv3 gene, was identified in the genotype of a complex interspecific hybrid of grapes Bianca. Later, it was found that this gene had seven haplotypes of resistance inherited from North American grape species, and that it was possible to identify the allelic status of the gene using DNA-markers UDV305, UDV737. However, only two haplotypes can be combined in one diploid form. To determine the Rpv3 gene in the grape gene pool we, using these markers, studied 35 different genotypes of grapevines, most of which are interspecies cultivars. Three varieties with known allelic status of the Rpv3 gene (Dunavski lazur, Noah, Seyve Villard 12-375) were included in the study as reference genotypes. The genotypes were studied through polymerase chain reaction with separation of amplification products by capillary electrophoresis in automatic genetic analyzer ABI Prism 3130. In the studied grape cultivars DNA marker analysis indentified the Rpv3 gene in sixteen genotypes of interspecific origin, including haplotype Rpv3299-279 found in twelve varieties, Rpv3321-312 – in three, and haplotype Rpv3null-271 – in one variety. Seyve Villard 12-375 turned out to be the donor of resistance gene in the most of the genotypes carrying Rpv3 in this study. The obtained data can be useful in selection of mildew resistant grape varieties and screening for hybridization pairs.

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Search for Rpv3 and Rpv12 genes in genotypes of table and seedless grape varieties using DNA-markers

Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020 ◽

10.28983/plamic2020.158 ◽

2020 ◽

Author(s):

M. V. Makarkina ◽

E. T. Ilnitskaya ◽

S. V. Tokmakov

Keyword(s):

Downy Mildew ◽

Resistance Genes ◽

Dna Markers ◽

Dna Marker ◽

Downy Mildew Resistance ◽

Mildew Resistance ◽

Marker Analysis ◽

Seedless Grape ◽

Seedless Grapes ◽

Grape Varieties

DNA-marker analysis of grape genotypes for the presence of downy mildew resistance genes Rpv3 and Rpv12 was performed. Table and seedless grapes varieties and forms carring these resistance genes were identified according to the DNA-marker evaluation.

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Assessment of powdery mildew (Blumeria graminis f. sp. hordei) resistance genes in Turkish barley varieties

Phytoprotection ◽

10.7202/000380ar ◽

2009 ◽

Vol 89 (1) ◽

pp. 31-36 ◽

Cited By ~ 2

Author(s):

Ahmet Zeybek ◽

Şahin Dere ◽

Gülay Gök ◽

Asude Çallak ◽

Mahinur S. Akkaya

Keyword(s):

Hordeum Vulgare ◽

Powdery Mildew ◽

Resistance Genes ◽

Blumeria Graminis ◽

Leaf Segment ◽

R Gene ◽

R Genes ◽

Resistant Varieties

Thirty-four Turkish barley (Hordeum vulgare) varieties were tested for the presence of resistance genes (R-genes) to powdery mildew (Blumeria graminisf. sp.hordei) using the “leaf segment test” with nine isolates of the fungus. The most commonly found R-gene wasMla8, while a combination of R-genesMlgandMl(CP)was found in three varieties (Tokak 157/37, Beysehir 98, Konevei 98). The geneMlhwas found in four varieties (Obruk 86, Anadolu 86, Çıldır 02, Özdemir 05), while no R-gene was found in three varieties (Hamidiye 85, Yesevi 93, Bülbül 89). No clear inferences could be made for the partly-resistant varieties Gemici 7243, Yea 793.12 and Akhisar 98 using the test isolates selected, which suggests the presence of still unidentified R-genes. Overall, 10 previously characterized R-genes for powdery mildew (Mla8,Ml(La),Mlg,Ml(CP),Mlh,Mlat,Mla1,Mlh,Mla7,Mlra) were present in Turkish barley varieties, along with some other(s) still to be identified.

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