IDENTIFICATION OF DONORS OF RESISTANCE GENES TO DOWNY MILDEW RPV3 FOR BREEDING OF TABLE GRAPE VARIETIES

2021 ◽  
Vol 31 ◽  
pp. 133-136
Author(s):  
E.T. ILNITSKAYA ◽  
◽  
M.V. MAKARKINA ◽  
T.D. KOZINA ◽  
◽  
...  
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Table Grape ◽  
Grape Varieties

scholarly journals DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

2020 ◽  
Vol 25 ◽  
pp. 03004
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Sergey Tokmakov ◽  
Victoriya Kotlyar
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Table Grape ◽  
Chemical Treatments ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Special Software ◽  
Grape Varieties

DNA markers are widely used in grapevine breeding to create forms with combined resistance genes. Downy mildew is one of the most common fungal diseases of the vine in the world. Growing grapevines with increased resistance allows to reduce the number of chemical treatments. The decrease in the use of pesticides is especially significant for viticulture of table varieties, since berries are directly consumed by humans for food. Currently, more than 20 resistance genes have been identified by molecular methods, and DNA markers for many genes have been developed. The genes Rpv3 (inherited from North American grape species) and Rpv12 (derived from V. amurensis) are among the most effective and have an additive effect. The study of 14 table grape varieties for the presence of the Rpv3 gene and 8 varieties for the presence of the Rpv12 gene was performed by using DNA-marker analysis. The analysis included varieties that could inherit these genes from the parent forms, according to their ancestry. The study was conducted using an automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner, DNA-markers were taken from literature sources. According to the results of DNA-marker analysis, 9 varieties were identified, including 2 seedless varieties, with the Rpv3299-279 allele in the genotypes, which determines resistance to downy mildew, and 3 table varieties with the Rpv12 gene in the genotypes. One table grape genotype was identified with Rpv3 and Rpv12.

scholarly journals Potential of genetic resistance of new table grape hybrids to fungal pathogens

2021 ◽  
Vol 34 ◽  
pp. 02001
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Valeriy Petrov
Keyword(s):  
Powdery Mildew ◽  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Fungal Pathogens ◽  
Genetic Resistance ◽  
Plasmopara Viticola ◽  
Table Grape ◽  
Table Grapes ◽  
Hybrid Forms

Downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) are the most common and economically significant fungal diseases in vineyards. The task of this work is to study the genotypes of new promising hybrid forms of table grapes for the presence of resistance genes to downy mildew (Rpv10 and Rpv3) and powdery mildew (Ren9) using DNA-markers. The study was carried out on table grape hybrids under the working names Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya and registered variety Liviya. The studied genes were analyzed using markers UDV305 and UDV737 (Rpv3), GF09-46 (Rpv10), CenGen6 (Ren9). The following cultivars were used as reference genotypes: Saperavi severnyi (carries Rpv10 gene) and Regent (Rpv3 and Ren9). It was established that Rpv3 gene is carried by hybrids Kishmish dubovskiy, Agat dubovskiy, Kurazh, Valensiya, Akelo, Gamlet, Dubovskiy rozovyi, Pestryi. Ren9 gene was found in Artek, Agat dubovskiy, Kurazh, Ispolin, Valensiya, Arabella, Gamlet, Dubovskiy rozovyi, Pestryi. The Rpv10 gene was not detected in any of the analyzed grapevine samples. genotypes Agat dubovskiy, Kurazh, Gamlet, Dubovskiy rozovyi, Pestryi, Valensiya carry Rpv3 and Ren9 genes simultaneously. These grapevines have an elegant bunch and large berries that are attractive to consumers.

scholarly journals Search for donors of powdery mildew resistance genes among seedless and table grape varieties

2021 ◽  
Vol 39 ◽  
pp. 02005
Author(s):  
Marina Makarkina ◽  
Elena Ilnitskaya ◽  
Tatiana Kozina
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Table Grape ◽  
Main Method ◽  
Chemical Treatments ◽  
Pesticide Treatment ◽  
Resistant Varieties ◽  
Allelic Status ◽  
Grape Varieties

Powdery mildew (Erysiphe necator) is one of the most common and economically significant diseases of grapes. Currently, the main method of controlling the disease is pesticide treatment. Breeding of resistant varieties is necessary to reduce chemical treatments. Currently, a number of grape resistance genes to powdery mildew and DNA markers for identification the allelic status of these genes are known. In a study to determine the presence of resistance loci Ren3 and Ren9, 25 genotypes of table grape varieties were analyzed, including 18 seedless varieties. DNA markers GF15-42, ScORGF15-02 were used to identify Ren3 gene, and CenGen6 – to identify Ren9 gene. DNA of cultivars Regent and Seyve Villard 12-375, which have resistance alleles, were used as positive controls. As a result of DNA marker analysis, it was determined that genotypes of table varieties Viking, Kodryanka, Moldova, Nadezhda AZOS, Original and seedless varieties Pamyati Smirnova, Kishmish Zaporozhskiy and Kishmish 342 carry loci of resistance to powdery mildew Ren3 and Ren9.

scholarly journals Search for Rpv3 and Rpv12 genes in genotypes of table and seedless grape varieties using DNA-markers

2020 ◽  
Author(s):  
M. V. Makarkina ◽  
E. T. Ilnitskaya ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Dna Marker ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Marker Analysis ◽  
Seedless Grape ◽  
Seedless Grapes ◽  
Grape Varieties

DNA-marker analysis of grape genotypes for the presence of downy mildew resistance genes Rpv3 and Rpv12 was performed. Table and seedless grapes varieties and forms carring these resistance genes were identified according to the DNA-marker evaluation.

scholarly journals DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

2020 ◽  
Vol 2 (3) ◽  
pp. 15-19
Author(s):  
E. T. Ilnitskaya ◽  
M. V. Makarkina ◽  
S. V. Tokmakov
Keyword(s):  
Downy Mildew ◽  
Resistance Gene ◽  
Resistance Genes ◽  
North American ◽  
Dna Marker ◽  
North American Species ◽  
Downy Mildew Resistance ◽  
Mildew Resistance ◽  
Seedless Grape ◽  
Grape Varieties

Table grapes are a valuable dietary product. Seedless grapes are in high demand among consumers. For this reason, the breeding of seedless varieties is one of the popular trends in modern viticulture, along with the production of environmentally friendly products. Downy mildew (Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni) is one of the most common fungal diseases of the grapevine. Most downy mildew resistant grape accessions belong to North American species like Vitis aestivalis Michx., V. berlandieri Planch., V. cinerea (Engelm. ex A. Gray) Engelm. ex Millard, V. riparia Michx., V. rupestris  Scheele, etc. The search for donors of resistance genes is an urgent task. Rpv3 is one of the most significant resistance genes from a number of North American grape varieties. The aim of this work is to identify the downy mildew resistance gene Rpv3 in seedless grape varieties by means of DNA-marker analysis. The grape varieties with rudimentary development of seed in berries and with North American species in the pedigree were chosen as the object of the study. The varieties “Dunavski lazur” and “Seyve Villard 12-375” with reference alleles were used as the positive control, while V. vinifera L. was used as the negative control. UDV305 and UDV737 DNA-markers were used in this study to identify the allelic type of the Rpv3 gene. The work was performed using the polymerase chain reaction. The reaction products were separated by capillary electrophoresis using the ABI Prism 3130 automatic genetic analyzer. Evaluation of the results was done using the GeneMapper and PeakScanner software. Functional alleles of the downy mildew resistance gene Rpv3 were revealed in grape  varieties “Kishmish zaporozhskiy”, “Lady Patricia”, “Remaily seedless”, “Pamyati Smirnova” and “Shayan”. Rpv3299-279, one of the seven known haplotypes, was identified in all the varieties. The pedigree analysis of the studied varieties indicated that the parental forms – “Seyve Villard” and “Seibel” hybrids – are presumably the donors of the gene. Grape accessions with the identified Rpv3 gene can be used in seedless varieties breeding as donors of resistance to downy mildew.

Colocation of downy mildew (Plasmopara halstedii) resistance genes in sunflower (Helianthus annuus L.)

Euphytica ◽  
1996 ◽  
Vol 91 (2) ◽  
pp. 225-228 ◽  
Author(s):  
Patricia Roeckel-Drevet ◽  
Geneviève Gagne ◽  
Said Mouzeyar ◽  
Laurent Gentzbittel ◽  
Jacqueline Philippon ◽  
...  
Keyword(s):  
Helianthus Annuus ◽  
Downy Mildew ◽  
Resistance Genes ◽  
Plasmopara Halstedii ◽  
Helianthus Annuus L

scholarly journals Estimating technical efficiency of Chinese table grape wholesalers

2015 ◽  
Vol 117 (6) ◽  
pp. 1670-1688 ◽  
Author(s):  
Weihua Jiao ◽  
Zetian Fu ◽  
Weisong Mu ◽  
Xiaoshuan Zhang ◽  
Jianjun Lu ◽  
...  
Keyword(s):  
Technical Efficiency ◽  
Agricultural Research ◽  
Table Grape ◽  
Scale Efficiency ◽  
Tobit Regression ◽  
Policy Makers ◽  
Content Type ◽  
Explanatory Variables ◽  
Parametric Data ◽  
Grape Varieties

Purpose – The purpose of this paper is to estimate the technical efficiency of Chinese table grape wholesalers and subsequently to examine the degree to which the calculated efficiency correlates with a set of explanatory variables. Design/methodology/approach – A two-stage procedure is applied in this paper. First, a non-parametric data envelopment analysis (DEA) technique is applied to investigate the degree of technical efficiency for Chinese table grape wholesalers. Second, Tobit regression is used to analyze the factors influencing technical efficiency. Findings – Research results reveal that the mean technical efficiency of the sample is 0.544 and 0.860 under constant returns scale (CRS) and VRS assumptions, respectively, and the scale efficiency (SE) is 0.620. The variables of experiences, number of grape varieties on sale, daily selling volumes and fixed sale ratio have a significant effect on technical efficiency, while the other exogenous variables do not affect the efficiency in any significant way. Research limitations/implications – The findings of this research are specific to table grape wholesalers in China, however, the method used in this study is transferrable and applicable to the study of similar problems in others countries. Originality/value – This research has yielded some interesting and original insights into the efficiency level of Chinese grape wholesalers and the factors that influence the level of efficiency. The findings have practical implications for Chinese agriculture policy makers, and are able to stimulate further research of a similar type in the international agricultural research community.

The influence of table grape rootstock and cultivar combinations on susceptibility to downy mildew

2018 ◽  
Vol 47 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Luciane Bertoletti Barros ◽  
Luiz Antonio Biasi ◽  
Odile Carisse ◽  
Louise Larissa May De Mio
Keyword(s):  
Downy Mildew ◽  
Table Grape
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