scholarly journals Marker controlled screening of resistant to red stele root rot (Rpf1 gene) strawberry selected forms

2021 ◽  
Vol 36 ◽  
pp. 01006
Author(s):  
Alexander Lyzhin ◽  
Irina Luk’yanchuk
Keyword(s):  
Resistance Gene ◽  
Dna Markers ◽  
Root Rot ◽  
Allelic Polymorphism ◽  
Heterozygous State ◽  
Breeding For Resistance ◽  
Homozygous Genotype ◽  
Red Stele ◽  
Initial Forms ◽  
Hybrid Forms

The results of the analysis of allelic polymorphism of the Rpf1 red stele root rot resistance gene in strawberry hybrid forms using diagnostic DNA markers were shown. The recessive homozygous genotype (rpf1rpf1) was identified in strawberry seedlings 932-29 (F. virginiana subsp. platypetala (Rydb.) Staudt × Feyerverk), 933-4 (F. virginiana subsp. platypetala (Rydb.) Staudt × Rubinovyy kulon), 62-7, 62-23 (Bylinnaya × Feyerverk), 65-2, 65-15 (Olimpiyskaya nadezhda × Bylinnaya) and 35-16 (922-67 × Maryshka). The heterozygous state of the Rpf1 gene was identified in strawberry forms 69-29 (Feyerverk × Bylinnaya), 62-41 (Bylinnaya × Feyerverk), 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 65-17, 65-24 (Olimpiyskaya nadezhda × Bylinnaya), which allows them to be used in breeding for resistance to P. fragariae var. fragariae as valuable initial forms.

scholarly journals Analysis of strawberry promising varieties and selected forms by resistance to red stele root rot using molecular markers

2021 ◽  
Vol 39 ◽  
pp. 02002
Author(s):  
Alexander Lyzhin ◽  
Irina Luk’yanchuk
Keyword(s):  
Molecular Markers ◽  
Root Rot ◽  
Heterozygous State ◽  
Breeding Work ◽  
Homozygous Genotype ◽  
Allelic State ◽  
Red Stele

The analysis of the allelic state of the Rpf1 red stele root rot gene in 14 promising foreign strawberry varieties and 6 selected forms of the I.V. Michurin FSC breeding was completed. The Rpf1 gene in a heterozygous state was identified in strawberry forms 61-15 (Bylinnaya × Olimpiyskaya nadezhda), 69-29 (Feyerverk × Bylinnaya), and 72-71 (Privlekatelnaya × Bylinnaya), which makes it possible to recommend them for involvement in breeding work to create resistant to P. fragariae var. fragariae strawberry varieties. Strawberry varieties Lebedushka, Elianny, Florence, Malwina, Monterey, Polka, Verona, Vima Tarda Asia, Chamora Turusi, Clery, Flamenco, Salsa and Symphony, and selected forms 56-5 (Gigantella Maxim × Privlekatelnaya), 69-42 (Feyerverk × Bylinnaya) and 35-16 (922-67 × Maryshka) have a recessive homozygous genotype.

scholarly journals Creation of new tomato forms with fungal disease resistance genes based on marker selection

2021 ◽  
pp. 16-21
Author(s):  
I. N. Shamshin ◽  
E. V. Grosheva ◽  
M. V. Maslova ◽  
R. M. Samoilova
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Fungal Diseases ◽  
Heterozygous State ◽  
Standard Type ◽  
Marker Selection ◽  
Young Leaves ◽  
Hybrid Forms

Relevance. The presented studies are aimed at obtaining new forms of tomato with a complex of genes for resistance to fungal diseases in combination with a standard type of bush and dark coloring of fruits based on marker-mediated selection.Methodology. The biological objects of the study are varieties and hybrid forms of tomato from the collection of the Michurinsky SAU. Molecular genetic analysis was performed using the following methods. DNA extraction was carried out from young leaves using a kit for isolation of NC Sample NC manufactured by Agrodiagnostika LLC according to the manufacturer's protocol. Fermentas production kits were used for PCR. Identification of the cladosporosis resistance gene was Cf-19 performed using the DNA marker R7. The presence of a fusarious wilting resistance gene was determined by a I-2/5 marker. The amplification results were visualized by agarose gel electrophoresis.Results. During the research, a collection of varieties and hybrid forms of tomato of the Michurinsky GAU was analyzed in order to identify genes for resistance to cladosporiosis Cf-19 and fusarium wilt I-2. A total of 52 genotypes were analyzed. It was found that most samples (41 samples) are characterized by a heterozygous state of the Cf-19 gene. All indeterminant and semi-determinant forms had both alleles. Of the 23 determinant forms presented in the collection, 10 had only one allele corresponding to recessive homozygote. Among all analyzed tomato genotypes, no dominant homozygous forms were noted. The study of the collection revealed several alleles of the I-2 gene. In total, four fragments corresponding to various alleles were amplified. A total of 50 resistant genotypes have been identified in the collection. Two alleys of the I-2 gene (633/693 bp) were identified in 42 tomato samples. Four varieties are homozygous in one allele (633 bp), which determines resistance. Three varieties have a second resistance allele (566 bp). One genotype has only an allele defining susceptibility (693 bp). On the basis of molecular analysis, as well as an assessment of the type of bush and fetal color, initial forms were selected with subsequent hybridization. 67 hybrid tomato plants were obtained. Evaluation of the presence of resistance genes showed that most of the resulting hybrids are resistant to cladosporiosis and fuzariosis. This is due to the presence of dominant alleles of Cf-19 and I-2 genes in a heterozygous state. Among the resulting hybrids, plants with a bark type of bush were identified. A total of 13 such plants were obtained.Conclusion. Thus, the work carried out allowed to obtain hybrid forms of tomato combine the signs of resistance to two pathogens of fungal diseases and the stem type of the bush. These forms are planned to be used in further selection work.

SCREENING OF STRAWBERRIES WITH THE RED STELE (PHYTOPHTHORA FRAGARIAE) RESISTANCE GENE RPF1 USING SEQUENCE SPECIFIC DNA MARKERS

2007 ◽  
pp. 165-169 ◽  
Author(s):  
A. Sasnauskas ◽  
R. Rugienius ◽  
D. Gelvonauskiené ◽  
I. Zalunskaitė ◽  
G. Stanienė ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Dna Markers ◽  
Phytophthora Fragariae ◽  
Red Stele

scholarly journals Analysis of strawberry genetic collection (Fragaria L.) for Rca2 and Rpfl genes with molecular markers

2018 ◽  
Vol 22 (7) ◽  
pp. 795-799 ◽  
Author(s):  
I. V. Luk’yanchuk ◽  
A. S. Lyzhin ◽  
I. I. Kozlova
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Resistance Gene ◽  
Wild Species ◽  
Root Rot ◽  
Scar Marker ◽  
Fragaria X Ananassa ◽  
Anthracnose Resistance ◽  
Monogenic Resistance ◽  
Red Stele

Strawberry (Fragaria x ananassa Duch.) varieties are susceptible to many fungal diseases. Identification of forms, carrying resistance genes, is an important stage in breeding programs leading to resistant varieties. The use of molecular markers allows to determine with high reliability the presence of the necessary genes in the genome and to identify promising forms. Some of the common strawberry's diseases, causing significant damage to strawberry plantations, are anthracnose (Colletotrichum acutatum Simmonds) and red stele root rot (Phytophthora fragariae var. fragariae Hickman). Dominant Rca2 gene is involved in monogenic resistance to C. acutatum pathogenicity group 2. Rpf1, Rpf2, Rpf3 genes are determined in monogenic resistance to red stele root rot. The purpose of this study was molecular genetic testing genotypes of genus Fragaria L. to identify carriers of Rca2 allele anthracnose resistance and Rpf1 allele red stele root rot resistance. The objects of study were the wild species of the genus Fragaria L. and strawberry varieties (Fragaria x ananassa Duch.) of different ecological and geographic origin. To assess allelic state Rca2 anthracnose resistance gene the dominant SCAR marker STS-Rca2_240 was used, was linked to the resistance gene Rca2 with a genetic distance of 2.8 cM. Rpf1 gene red stele root rot resistance was identified with the dominant SCAR marker R1A, was linked to the resistance gene Rpf1 with a genetic distance of 3.0 cM. The resistant allele of the marker STS-Rca2_240 was identified in the Laetitia variety (Rca2Rca2 or Rca2rca2 genotype), which allows us to recommend it as a promising source in breeding for anthracnose resistance. The other studied forms have homozygous recessive state of the marker STS-Rca2_240 (putative genotype rca2rca2). The resistant allele of the marker SCAR-R1A in the varieties and wild species of strawberry under study is absent, which presumably indicates their homozygous recessive genotype of Rpf1 gene (rpf1rpf1).

Detection of the resistance gene Rpv3 in grapevine cultivars using DNA- markers

2017 ◽  
Vol 1 (67) ◽  
pp. 63-67
Author(s):  
Elena Ilnitskaya ◽  
◽  
Sergey Tokmakov ◽  
Marina Makarkina ◽  
◽  
...  
Keyword(s):  
Resistance Gene ◽  
Dna Markers

Breeding for Resistance to Alfalfa Root Rot Caused by Fusarium Species

Crop Science ◽  
1994 ◽  
Vol 34 (5) ◽  
pp. 1213-1217 ◽  
Author(s):  
R. Salter ◽  
J. E. Miller‐Garvin ◽  
D. R. Viands
Keyword(s):  
Root Rot ◽  
Fusarium Species ◽  
Breeding For Resistance ◽  
Alfalfa Root

Analysis of rice blast resistance gene Pi-z in rice germplasm using pathogenicity assays and DNA markers

Euphytica ◽  
2011 ◽  
Vol 184 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Moytri RoyChowdhury ◽  
Yulin Jia ◽  
Aaron Jackson ◽  
Melissa H. Jia ◽  
Robert Fjellstrom ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Dna Markers ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Rice Germplasm

scholarly journals Potential of genetic resistance of new table grape hybrids to fungal pathogens

2021 ◽  
Vol 34 ◽  
pp. 02001
Author(s):  
Elena Ilnitskaya ◽  
Marina Makarkina ◽  
Valeriy Petrov
Keyword(s):  
Powdery Mildew ◽  
Downy Mildew ◽  
Resistance Genes ◽  
Dna Markers ◽  
Fungal Pathogens ◽  
Genetic Resistance ◽  
Plasmopara Viticola ◽  
Table Grape ◽  
Table Grapes ◽  
Hybrid Forms

Downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) are the most common and economically significant fungal diseases in vineyards. The task of this work is to study the genotypes of new promising hybrid forms of table grapes for the presence of resistance genes to downy mildew (Rpv10 and Rpv3) and powdery mildew (Ren9) using DNA-markers. The study was carried out on table grape hybrids under the working names Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya and registered variety Liviya. The studied genes were analyzed using markers UDV305 and UDV737 (Rpv3), GF09-46 (Rpv10), CenGen6 (Ren9). The following cultivars were used as reference genotypes: Saperavi severnyi (carries Rpv10 gene) and Regent (Rpv3 and Ren9). It was established that Rpv3 gene is carried by hybrids Kishmish dubovskiy, Agat dubovskiy, Kurazh, Valensiya, Akelo, Gamlet, Dubovskiy rozovyi, Pestryi. Ren9 gene was found in Artek, Agat dubovskiy, Kurazh, Ispolin, Valensiya, Arabella, Gamlet, Dubovskiy rozovyi, Pestryi. The Rpv10 gene was not detected in any of the analyzed grapevine samples. genotypes Agat dubovskiy, Kurazh, Gamlet, Dubovskiy rozovyi, Pestryi, Valensiya carry Rpv3 and Ren9 genes simultaneously. These grapevines have an elegant bunch and large berries that are attractive to consumers.

scholarly journals Assessment of the adaptive potential of strawberry genotypes based on the development of endophytic microbiota and identified R-genes

2021 ◽  
Vol 845 (1) ◽  
pp. 012023
Author(s):  
IV Luk’yanchuk ◽  
K V Zaitseva ◽  
A S Lyzhin
Keyword(s):  
Root Rot ◽  
High Frequency ◽  
Fungal Pathogens ◽  
R Genes ◽  
Genetic Determinants ◽  
Adaptive Potential ◽  
Anthracnose Resistance ◽  
Bacterial Microbiota ◽  
Wild Strawberry ◽  
Red Stele

Abstract The paper presents the results of a study of the strawberry genotypes (Fragaria L.) by characteristics of endophytic microbiota and genetic determinants of resistance to fungal pathogens. Highly adaptive genotypes were identified: F. virginiana Duch. ssp. platypetala, F. orientalis Los., F. ovalis Rydb., F. moschata Duch., 298-19-9-43, Urozhaynaya CGL, Flora, Privlekatelnaya and Troitskaya. These strawberry forms are characterized by a high frequency of testing of bacterial microbiota, which suppresses endophytic fungal pathogens. The wild strawberry F. virginiana Duch. ssp. platypetala is characterized by red stele root rot resistance (Rpf1 gene). Strawberry varieties Borovitskaya and Elianny are characterized by anthracnose resistance (Rca2gene).

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