scholarly journals Analysis of strawberry promising varieties and selected forms by resistance to red stele root rot using molecular markers

2021 ◽  
Vol 39 ◽  
pp. 02002
Author(s):  
Alexander Lyzhin ◽  
Irina Luk’yanchuk
Keyword(s):  
Molecular Markers ◽  
Root Rot ◽  
Heterozygous State ◽  
Breeding Work ◽  
Homozygous Genotype ◽  
Allelic State ◽  
Red Stele

The analysis of the allelic state of the Rpf1 red stele root rot gene in 14 promising foreign strawberry varieties and 6 selected forms of the I.V. Michurin FSC breeding was completed. The Rpf1 gene in a heterozygous state was identified in strawberry forms 61-15 (Bylinnaya × Olimpiyskaya nadezhda), 69-29 (Feyerverk × Bylinnaya), and 72-71 (Privlekatelnaya × Bylinnaya), which makes it possible to recommend them for involvement in breeding work to create resistant to P. fragariae var. fragariae strawberry varieties. Strawberry varieties Lebedushka, Elianny, Florence, Malwina, Monterey, Polka, Verona, Vima Tarda Asia, Chamora Turusi, Clery, Flamenco, Salsa and Symphony, and selected forms 56-5 (Gigantella Maxim × Privlekatelnaya), 69-42 (Feyerverk × Bylinnaya) and 35-16 (922-67 × Maryshka) have a recessive homozygous genotype.

scholarly journals Marker controlled screening of resistant to red stele root rot (Rpf1 gene) strawberry selected forms

2021 ◽  
Vol 36 ◽  
pp. 01006
Author(s):  
Alexander Lyzhin ◽  
Irina Luk’yanchuk
Keyword(s):  
Resistance Gene ◽  
Dna Markers ◽  
Root Rot ◽  
Allelic Polymorphism ◽  
Heterozygous State ◽  
Breeding For Resistance ◽  
Homozygous Genotype ◽  
Red Stele ◽  
Initial Forms ◽  
Hybrid Forms

The results of the analysis of allelic polymorphism of the Rpf1 red stele root rot resistance gene in strawberry hybrid forms using diagnostic DNA markers were shown. The recessive homozygous genotype (rpf1rpf1) was identified in strawberry seedlings 932-29 (F. virginiana subsp. platypetala (Rydb.) Staudt × Feyerverk), 933-4 (F. virginiana subsp. platypetala (Rydb.) Staudt × Rubinovyy kulon), 62-7, 62-23 (Bylinnaya × Feyerverk), 65-2, 65-15 (Olimpiyskaya nadezhda × Bylinnaya) and 35-16 (922-67 × Maryshka). The heterozygous state of the Rpf1 gene was identified in strawberry forms 69-29 (Feyerverk × Bylinnaya), 62-41 (Bylinnaya × Feyerverk), 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 65-17, 65-24 (Olimpiyskaya nadezhda × Bylinnaya), which allows them to be used in breeding for resistance to P. fragariae var. fragariae as valuable initial forms.

scholarly journals Analysis of strawberry genetic collection (Fragaria L.) for Rca2 and Rpfl genes with molecular markers

2018 ◽  
Vol 22 (7) ◽  
pp. 795-799 ◽  
Author(s):  
I. V. Luk’yanchuk ◽  
A. S. Lyzhin ◽  
I. I. Kozlova
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Resistance Gene ◽  
Wild Species ◽  
Root Rot ◽  
Scar Marker ◽  
Fragaria X Ananassa ◽  
Anthracnose Resistance ◽  
Monogenic Resistance ◽  
Red Stele

Strawberry (Fragaria x ananassa Duch.) varieties are susceptible to many fungal diseases. Identification of forms, carrying resistance genes, is an important stage in breeding programs leading to resistant varieties. The use of molecular markers allows to determine with high reliability the presence of the necessary genes in the genome and to identify promising forms. Some of the common strawberry's diseases, causing significant damage to strawberry plantations, are anthracnose (Colletotrichum acutatum Simmonds) and red stele root rot (Phytophthora fragariae var. fragariae Hickman). Dominant Rca2 gene is involved in monogenic resistance to C. acutatum pathogenicity group 2. Rpf1, Rpf2, Rpf3 genes are determined in monogenic resistance to red stele root rot. The purpose of this study was molecular genetic testing genotypes of genus Fragaria L. to identify carriers of Rca2 allele anthracnose resistance and Rpf1 allele red stele root rot resistance. The objects of study were the wild species of the genus Fragaria L. and strawberry varieties (Fragaria x ananassa Duch.) of different ecological and geographic origin. To assess allelic state Rca2 anthracnose resistance gene the dominant SCAR marker STS-Rca2_240 was used, was linked to the resistance gene Rca2 with a genetic distance of 2.8 cM. Rpf1 gene red stele root rot resistance was identified with the dominant SCAR marker R1A, was linked to the resistance gene Rpf1 with a genetic distance of 3.0 cM. The resistant allele of the marker STS-Rca2_240 was identified in the Laetitia variety (Rca2Rca2 or Rca2rca2 genotype), which allows us to recommend it as a promising source in breeding for anthracnose resistance. The other studied forms have homozygous recessive state of the marker STS-Rca2_240 (putative genotype rca2rca2). The resistant allele of the marker SCAR-R1A in the varieties and wild species of strawberry under study is absent, which presumably indicates their homozygous recessive genotype of Rpf1 gene (rpf1rpf1).

Mapping of rhizoctonia root rot resistance genes in sugar beet using pathogen response-related sequences as molecular markers

2008 ◽  
Vol 127 (6) ◽  
pp. 602-611 ◽  
Author(s):  
J. C. Lein ◽  
C. M. Sagstetter ◽  
D. Schulte ◽  
T. Thurau ◽  
M. Varrelmann ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Genes ◽  
Root Rot ◽  
Rhizoctonia Root Rot ◽  
Pathogen Response

Identification and development of molecular markers linked to Phytophthora root rot resistance in pepper (Capsicum annuum L.)

2012 ◽  
Vol 135 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Hai Thi Hong Truong ◽  
Jeong Ho Kim ◽  
Myoung Cheoul Cho ◽  
Soo Young Chae ◽  
Hye Eun Lee
Keyword(s):  
Molecular Markers ◽  
Capsicum Annuum ◽  
Root Rot ◽  
Phytophthora Root Rot ◽  
Capsicum Annuum L

scholarly journals Assessment of the adaptive potential of strawberry genotypes based on the development of endophytic microbiota and identified R-genes

2021 ◽  
Vol 845 (1) ◽  
pp. 012023
Author(s):  
IV Luk’yanchuk ◽  
K V Zaitseva ◽  
A S Lyzhin
Keyword(s):  
Root Rot ◽  
High Frequency ◽  
Fungal Pathogens ◽  
R Genes ◽  
Genetic Determinants ◽  
Adaptive Potential ◽  
Anthracnose Resistance ◽  
Bacterial Microbiota ◽  
Wild Strawberry ◽  
Red Stele

Abstract The paper presents the results of a study of the strawberry genotypes (Fragaria L.) by characteristics of endophytic microbiota and genetic determinants of resistance to fungal pathogens. Highly adaptive genotypes were identified: F. virginiana Duch. ssp. platypetala, F. orientalis Los., F. ovalis Rydb., F. moschata Duch., 298-19-9-43, Urozhaynaya CGL, Flora, Privlekatelnaya and Troitskaya. These strawberry forms are characterized by a high frequency of testing of bacterial microbiota, which suppresses endophytic fungal pathogens. The wild strawberry F. virginiana Duch. ssp. platypetala is characterized by red stele root rot resistance (Rpf1 gene). Strawberry varieties Borovitskaya and Elianny are characterized by anthracnose resistance (Rca2gene).

Molecular Markers Linked to Phytophthora fragariae Resistance Genes in Strawberry

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 500a-500 ◽  
Author(s):  
K.M. Haymes ◽  
S.C. Hokanson ◽  
K. Salazar ◽  
J. Maas
Keyword(s):  
Molecular Markers ◽  
Resistance Genes ◽  
Bulked Segregant Analysis ◽  
Aflp Markers ◽  
Avirulence Genes ◽  
Phytophthora Fragariae ◽  
Red Stele ◽  
Strawberry Breeding ◽  
Direct Applicability ◽  
Selection Of

The commercial strawberry (Fragaria × ananassa Duch.) is susceptible to red stele root rot caused by the fungus Phytophthora fragariae Hickman var. fragariae. Characteristics of the disease are the reddened steles and “rat tail” appearance of the roots. Infected plants are dwarfed, exhibit wilting, and eventually die. Red stele resistance in strawberries and virulence of P. fragariae behave according to the gene-for-gene system. Resistance genes and their corresponding avirulence genes have been identified by screening plant roots for infection. The goal of this international research project with CPRO-DLO, The Netherlands, is to develop highly specific molecular markers for various Rpf resistance genes (Rpf1, Rpf2, Rpf3, and Rpf6) that confer resistance against P. fragariae. Bulked Segregant Analysis was used to identify RAPD and AFLP markers putatively linked to P. fragariae resistance genes. The bulked DNAs representing subsets of three F1 populations that segregated monogenically for either resistance or susceptibility to P. fragariae. The map of these Rpf loci was generated using JoinMap®. The RAPD markers linked to the resistance genes are being converted into SCAR markers, while the AFLP markers will be used as probes for the detection of P. fragariae. The application of the molecular markers linked to resistance genes will have direct applicability to strawberry breeding programs. Marker-facilitated selection of these resistance genes would allow an efficient means in the screening and selection of plant material containing these genes and help in the pyramiding for resistance to P. fragariae. Genetic improvement of the strawberry by the ability to pyramid resistance genes will contribute to the productivity of the strawberry industry worldwide.

Linked morphological and molecular markers associated with common root rot reaction in barley

1996 ◽  
Vol 76 (4) ◽  
pp. 879-883 ◽  
Author(s):  
H. R. Kutcher ◽  
K. L. Bailey ◽  
B. G. Rossnagel ◽  
J. D. Franckowiak
Keyword(s):  
Molecular Markers ◽  
Root Rot ◽  
Rapd Marker ◽  
Cochliobolus Sativus ◽  
Common Root ◽  
Morphological Marker ◽  
Common Root Rot ◽  
Barley Germplasm ◽  
Efficiency Of Selection ◽  
Selection Of

Selection of common root rot [Cochliobolus sativus (Ito & Kurib.) Drechsl. ex Dastur] resistant barley germplasm is difficult due to the influence of environment on disease expression and the quantitative nature of resistance. Morphological and molecular markers associated with common root rot resistance could be used to increase the efficiency of selection of resistant germplasm and facilitate transfer to desirable barley genotypes Forty-five morphological marker sets consisting of four to six backcross-derived lines for each marker in a common background (cultivar Bowman) were planted in disease nurseries at two locations in each of 3 yr. Disease reactions of the back-cross-derived lines were tested for a difference from the disease reaction of Bowman, which indicated a putative association between the marker and common root rot reaction. An association was obtained between common root rot reaction and one set of backcross-derived lines that contained two linked markers: glossy-sheath (gs4) and orange lemma (o). A RAPD marker closely linked to gs4 and o was also associated with common root rot reaction. The RAPD marker was associated with the allele for resistance, while gs4 and o were associated with the allele for susceptibility. The RAPD marker would be most useful in the selection of common root rot resistant germplasm and the transfer of this resistance to other genotypes. Key words: Common root rot, Cochliobolus sativus, barley, morphological markers, molecular markers

scholarly journals A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis

OENO One ◽  
2021 ◽  
Vol 55 (2) ◽  
pp. 403-414
Author(s):  
Frederique Pelsy ◽  
Lucie Bevilacqua ◽  
Sophie Blanc ◽  
Didier Merdinoglu
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Phylogenetic Relationships ◽  
Genetic Material ◽  
Dna Polymorphisms ◽  
Heterozygous State ◽  
Empty Site ◽  
Microsatellite Sequence ◽  
Vitis Species ◽  
Ssr Polymorphism

Molecular markers, based on DNA polymorphisms, are useful tools for identifying individuals, establishing phylogenetic relationships, managing collections of genetic material or assisting breeding. In the present study, we developed a marker set to differentiate Vitis species, grapevine varieties or clones belonging to the same variety. This novel marker set combines, in four PCR amplifications, the presence/absence of a remarkable retrotransposon, Tvv1-Δ3460, inserted at its single locus and the SSR polymorphism present within its two LTRs. By studying a collection of Vitaceaeaccessions, we showed the prevalence of two allelic forms of Tvv1-Δ3460 - one of which was partially truncated - in Vitis species. Out of the twenty-five studied Vitis species, the insertion of a Tvv1-Δ3460 element was detected in twenty, including Vitis vinifera. The homozygous vs heterozygous state of the element insertion was determined by amplifying the empty site. Additionally, each Tvv1-Δ3460 LTRs included a microsatellite sequence useful for designing markers based on LTR length. The LTR-SSR markers distinguished most of the fifty-two cultivars and revealed polymorphism within five of the seven varieties studied.

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