scholarly journals Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-SB3-HBcAg

2021 ◽  
Vol 41 ◽  
pp. 07003
Author(s):  
Lalu Unsunnidhal ◽  
Raudatul Jannah ◽  
Abdul Haris ◽  
Agus Supinganto ◽  
Asmarani Kusumawati
Keyword(s):  
Hepatitis B ◽  
Restriction Analysis ◽  
Negative Control ◽  
Core Antigen ◽  
Base Sequence ◽  
E Coli ◽  
Particle Size Analyzer ◽  
B Virus ◽  
Good Potential ◽  
Hepatitis B Core Antigen

Hepatitis B virus (HBV) is a DNA virus that causes hepatitis in humans. This study aims to prepare a Hepatitis DNA vaccine. he optimized base sequence of the SB3-HBcAg gene was derived from the nucleotide base sequence of the Hepatitis B core antigen B3 HBcAg subgenotype, and then Cloning of the pcDNA3.1-SB3-HBcAg has been successfully performed on E. coli DH5α and confirmed by PCR, restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and physiochemical characterized using Particle Size Analyzer. Complex with a 4:1 (wt/wt) ratio of DNA with 0.04% concentration and chitosan have a mean diameter of 231.7 nm and zeta potential +12.3 mV and the value of Cytotoxicity Assay 80-90% as compared to the untreated cells that used as negative control, so it can be concluded that nanoparticles chitosan has good potential as a carrier agent for pcDNA3.1-SB3-HBcAg.

scholarly journals Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-tat

2021 ◽  
Vol 41 ◽  
pp. 07004
Author(s):  
Lalu Unsunnidhal ◽  
Raden Wasito ◽  
Erif Maha Nugraha Setyawan ◽  
Asmarani Kusumawati
Keyword(s):  
Restriction Analysis ◽  
Protein Sequences ◽  
Negative Control ◽  
Tat Protein ◽  
Consensus Process ◽  
Cattle Industry ◽  
E Coli ◽  
Particle Size Analyzer ◽  
Mean Diameter ◽  
Good Potential

The development of Jembrana disease vaccine is importance to prevent the loss of Bali cattle industry in Indonesia. This study aims to prepare a Jembrana DNA vaccine. The data Tat protein sequences gained from NCBI and the consensus process has been finished by the MultAlign program, and then Cloning of the pcDNA3.1-tat has been successfully performed on E. coli DH5α and confirmed by PCR, restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and physiochemical characterized using Particle Size Analyzer. Complex with a 1:2 (wt/wt) ratio of DNA and chitosan have a mean diameter of 268.5 nm and zeta potential +25.1 mV and the value of Cytotoxicity Assay 80-90% as compared to the untreated cells that used as negative control, so it can be concluded that nanoparticles chitosan has good potential as a carrier agent for pcDNA3.1-tat.

scholarly journals Hepatitis B Virus DNA in Persons with Isolated Antibody to Hepatitis B Core Antigen Who Subsequently Received Hepatitis B Vaccine

1998 ◽  
Vol 26 (4) ◽  
pp. 895-897 ◽  
Author(s):  
A. Eduardo Silva ◽  
Brian J. McMahon ◽  
Alan J. Parkinson ◽  
Maria H. Sjogren ◽  
Jay H. Hoofnagle ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hepatitis B Vaccine ◽  
Core Antigen ◽  
Hepatitis B Virus Dna ◽  
B Virus ◽  
Hepatitis B Core Antigen

scholarly journals Studies on IgA antibody to hepatitis B core antigen in patients with acute and chronic hepatitis B virus infection.

Kanzo ◽  
1985 ◽  
Vol 26 (6) ◽  
pp. 689-695
Author(s):  
Mitsuo SUGA ◽  
Yoshikazu AKAHONAI ◽  
Haruyasu YOSHIZAKI ◽  
Tetsuo OHSHIMA ◽  
Akira YACHI
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Antigen ◽  
Hepatitis B Virus Infection ◽  
Chronic Hepatitis B Virus ◽  
B Virus ◽  
Iga Antibody ◽  
Hepatitis B Core Antigen ◽  
Acute And Chronic Hepatitis

scholarly journals Relationship between the prevalence of antibodies to hepatitis B core antigen and arbovirus in fishermen from the Ribeira Valley, Brazil

1990 ◽  
Vol 32 (3) ◽  
pp. 215-220 ◽  
Author(s):  
Lygia Busch Iversson ◽  
Celso F. Hernandes Granato ◽  
Amélia Travassos da Rosa ◽  
Cláudio Sérgio Pannuti
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Age Groups ◽  
Sao Paulo ◽  
Core Antigen ◽  
São Paulo ◽  
Region Detection ◽  
Significant Difference ◽  
B Virus ◽  
Hepatitis B Core Antigen

Sera from 299 fishermen 16 to 80 years old, residents in Cananeia and Iguape counties, southern cost of São Paulo State, Brazil, were studied in order to identify a possible association between the prevalence of specific antibodies to the hepatitis B virus (HBV) and exposure to haematophagus mosquitoes evaluated by the prevalence of arbovirus antibodies. This professional group presented the highest prevalence of arbovirus antibodies (54.1%) in past investigations carried out in this heavily forested region. Detection of antibody to hepatitis B core antigen (anti-HBc) in the sera was done by enzyme immunoassay (Roche). Prevalence of anti-HBc antibodies in this group was 31.4% (94/299) which is very high compared with 7.2% to 15.0% for different groups of healthy adults in State of São Paulo. No significant difference is observed between the prevalences of HBV antibodies in Iguape and Cananeia. Prevalence of anti-HBc and anti-arbovirus antibodies increases with age. There is a concordance in the distribution according to age groups of the frequency of anti-HBc and anti-arbovirus positive sera. Ag HBs was detected in 4% of the studied sera. These results support the hypothesis that the transmission of the hepatitis B virus and the arboviruses may be due to the same factor, one of the possibilities would be by anthropophilic mosquitoes.

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