Nutritional supplement for indigenous intestinal microflora: manufacturing, quality and effectiveness evaluation

The pharmaceutical composition of a nutritional supplement, which has a profound effect on the maintenance and development of the indigenous intestinal microflora, has been scientifically proven. The nutritional supplement is obtained by combining the encapsulation technology with the pellet technology to ensure that the pharmaceutical composition remains intact and the product appeals to consumers. The combination of encapsulation and pellet technology is highly beneficial to the manufacturing process as the delivery of the ingredients, which are very different in absorption and penetration rates, is improved, controlled and becomes very site-specific, while at the same time offering even distribution. The quality of the nutritional supplement has been proven and the product complies with the compulsory sanitary-hygienic and sanitarytoxicological regulations for dietary supplements. Clinical trials for the new nutritional supplement were performed to ensure the safety and the effectiveness of the product. Sixty volunteers aged from 33 to 72 were involved in the clinical trials. All of the volunteers were diagnosed with different intestinal microbiota disorders, among them a decreased number of microbial symbionts (bifidobacteria, lactobacilli, Escherichia coli (Lac +), the presence of Klebsiella pnenmoniae and opportunistic pathogens like Candida alticans. Patients with intestinal microbiota disorders were prescribed to take 1 capsule of the nutritional supplement twice a day for a period of 30 days together with other medicine prescribed to improve the biocenosis. Having taken part in the clinical trials, nearly 90% of patients, who had previously experienced such symptoms as bloating, gas, different abdominal pains, cramping, diarrhoea and constipation, demonstrated microbiota restoration, along with the overall health improvement. Considering the findings of the clinical trials, it should be noted that the use of ‘Panbiom’ nutritional supplement can play an important role in keeping the immune system healthy, with short chain fatty acids becoming the main focus of attention. Short chain fatty acids are the key to stimulating the immune response, as they support the regulatory T cells, prevent pathological changes and lessen the symptoms. The nutritional supplement composition and the manufacturing process were developed and tested at the scientific research-to-production facilities of Art-Life Scientific Production Association in Tomsk. All the mandatory documentation was obtained and fully complies with the requirements for nutritional supplements.

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Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Zaporozhye Medical Journal ◽

10.14739/2310-1210.2021.5.231265 ◽

2021 ◽

Vol 23 (5) ◽

pp. 683-690

Author(s):

N. V. Vorobiova ◽

O. V. Usachova ◽

A. H. Kaplaushenko

Keyword(s):

Fatty Acids ◽

Intestinal Microbiota ◽

Metabolic Activity ◽

Intestinal Microflora ◽

Short Chain Fatty Acids ◽

Short Chain ◽

Malabsorption Syndrome ◽

Rotavirus Infection ◽

Carbohydrate Malabsorption ◽

Chain Fatty Acids

The aim is to evaluate the effect of metabolic activity of intestinal microflora on laboratory manifestations of carbohydrate malabsorption syndrome in early-aged children with rotavirus infection by studying the main intestinal metabolites – short-chain fatty acids in faeces. Materials and methods. 60 breastfed children with rotavirus infection aged 1–24 months were examined. The total amount of carbohydrates in faecal samples was determined using Benedict’s test, and the detection of short-chain fatty acids in faeces was carried out using liquid chromatography in the dynamics of the disease – on the 3rd, 5th and 10th day. Results. It was established, that the amount of reducing sugars in faeces decreased with the increasing concentration of intestinal microflora metabolites during the entire period of rotavirus infection. In patients with ≤0.5 % level of carbohydrates in faeces, the total content of short-chain fatty acids was the highest and exceeded by 2.0, 1.8 and 1.7 times the indicators of children with Benedict’s test values >0.5 % on the 3rd, 5th and 10th days of the disease, respectively (p < 0.05). A decrease in the metabolic activity of the intestinal microbiota in the above category of children occurred mainly due to a deficiency in the production of acetic and propionic acids (P < 0.05). In the acute period of the disease, an imbalance in the intestinal microbiota infrastructure was noted towards a deficiency of obligate anaerobes that was expressed in a decrease in the anaerobic index (P < 0.05). The severity of these disorders influenced the severity of carbohydrate malabsorption: with an increase in the level of undigested sugars in faeces >0.5 %, the anaerobic index values were 3 and 5 times lower than at a carbohydrate level ≤0.5 % on the 3rd and 5th days of the disease, respectively (P < 0.05). Conclusions. It has been found that early-aged children with rotavirus infection have structural and functional disorders of the intestinal microflora, which influence the severity of carbohydrate malabsorption syndrome. The lower the saccharolytic activity of intestinal bacteria, the more pronounced the manifestations of this syndrome during the entire period of the disease.

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