scholarly journals Bacillus subtilis for biological protection of Taxus baccata L. in landscape gardens

2021 ◽  
Vol 285 ◽  
pp. 02002
Author(s):  
Sergey Kopyltsov ◽  
Anna Gneush
Keyword(s):  
Bacillus Subtilis ◽  
Phytopathogenic Fungus ◽  
Taxus Baccata ◽  
Coniferous Tree ◽  
Rapd Pcr ◽  
Biological Protection ◽  
Fungistatic Effect

The fungistatic effect of the endophytic strain of Bacillus subtilis in park stands of Taxus baccata L. var. “Fastigiata”. The study used trees in park stands with symptoms of damage by the phytopathogenic fungus Cryptocline taxicola (All.) Petr. It was found that treatment of shoots with a suspension of microorganisms Bacillus subtillis strain krd-20 in the autumn and spring prevented the development of the disease and the fall of needles. RAPD-PCR was used to determine that the strain was capable of endophytic development and was preserved in the coniferous tree for at least six months.

scholarly journals Biological protection of plum from shot hole disease in the humid subtropics of the Krasnodar region (Russia)

2020 ◽  
Vol 21 ◽  
pp. 00035
Author(s):  
Nikolai Leonov ◽  
Timur Bulgakov
Keyword(s):  
Bacillus Subtilis ◽  
Research Centre ◽  
Phytopathogenic Fungus ◽  
Federal Research ◽  
Experimental Base ◽  
Krasnodar Region ◽  
Biological Protection ◽  
Different Strains ◽  
Academy Of Sciences ◽  
Humid Subtropics

The article considers the biologization of plum trees protection system from shot hole disease caused by the phytopathogenic fungus Wilsonomyces carpophilus in the humid subtropics of the Krasnodar region. Several biological fungicides based on different strains of the bacterium Bacillus subtilis (Alirin-B®, Bactophyt®, Gamair®, Phytosporin-M®, Vitaplan®), the bacterium Pseudomonas fluorescens (Rhizoplan®) and the fungus Trichoderma harzianum (Glyocladin® and Trichocin®) were tested on Stanley plum trees at the experimental base of the Federal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences (Sochi) in 2015–2017. According to seasonal features of shot hole disease and the results of experiments, the optimal timetable schedule for plum treatment with the fungicides was found (3 treatments in May–June).

scholarly journals Genotyping and Toxigenic Potential of Bacillus subtilis and Bacillus pumilus Strains Occurring in Industrial and Artisanal Cured Sausages

2004 ◽  
Vol 70 (9) ◽  
pp. 5168-5176 ◽  
Author(s):  
Alessandra Matarante ◽  
Federico Baruzzi ◽  
Pier Sandro Cocconcelli ◽  
Maria Morea
Keyword(s):  
Bacillus Subtilis ◽  
Dna Sequences ◽  
Bacillus Pumilus ◽  
Meat Products ◽  
Rrna Gene ◽  
Emerging Pathogens ◽  
Specific Pcr ◽  
Gene Sequence Analysis ◽  
Rapd Pcr ◽  
Cured Meat

ABSTRACT Artisanal and industrial sausages were analyzed for their aerobic, heat-resistant microflora to assess whether new emerging pathogens could be present among Bacillus strains naturally contaminating cured meat products. Sixty-four isolates were characterized by randomly amplified polymorphic DNA (RAPD)-PCR and fluorescent amplified fragment length polymorphism (fAFLP). The biotypes, identified by partial 16S rRNA gene sequence analysis, belonged to Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens species. Both RAPD-PCR and fAFLP analyses demonstrated that a high genetic heterogeneity is present in the B. subtilis group even in strains harvested from the same source, making it possible to isolate 56 different biotypes. Moreover, fAFLP analysis made it possible to distinguish B. subtilis from B. pumilus strains. The strains were characterized for their toxigenic potential by molecular, physiological, and immunological techniques. Specific PCR analyses revealed the absence of DNA sequences related to HBL, BcET, NHE, and entFM Bacillus cereus enterotoxins and the enzymes sphingomyelinase Sph and phospholipase PI-PLC in all strains; also, the immunological analyses showed that Bacillus strains did not react with NHE- and HBL-specific antibodies. However, some isolates were found to be positive for hemolytic and lecithinase activity. The absence of toxigenic potential in Bacillus strains from the sausages analyzed indicates that these products can be considered safe under the processing conditions they were produced; however, great care should be taken when the ripening time is shortened, particularly in the case of traditional sausages, which could contain high amounts of Bacillus strains and possibly some B. cereus cells.

scholarly journals SURFACTIN BACILLUS SUBTILIS 26D IN WHEAT PROTECTION FROM THE PHYTOPATHOGENIC FUNGUS STAGONOSPORA NODORUM (BERK.)

ÈKOBIOTEH ◽  
2019 ◽  
Vol 2 (3) ◽  
pp. 339-346
Author(s):  
E.A. Cherepanovа ◽  
◽  
D.K. Blagova ◽  
G.F. Burkhanova ◽  
E.S. Sarvarova ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stagonospora Nodorum ◽  
Phytopathogenic Fungus

Application of Bacillus subtilis in the biological control of the phytopathogenic fungus Sporisorium reilianum

2014 ◽  
Vol 76 ◽  
pp. 36-40 ◽  
Author(s):  
Y. Mercado-Flores ◽  
I.O. Cárdenas-Álvarez ◽  
A.V. Rojas-Olvera ◽  
J.P. Pérez-Camarillo ◽  
S.G. Leyva-Mir ◽  
...  
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Phytopathogenic Fungus ◽  
Sporisorium Reilianum

Antifungal efficiency of a lipopeptide biosurfactant derived from Bacillus subtilis SPB1 versus the phytopathogenic fungus, Fusarium solani

2015 ◽  
Vol 22 (22) ◽  
pp. 18137-18147 ◽  
Author(s):  
Ines Mnif ◽  
Ines Hammami ◽  
Mohamed Ali Triki ◽  
Manel Cheffi Azabou ◽  
Semia Ellouze-Chaabouni ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Solani ◽  
Phytopathogenic Fungus ◽  
Antifungal Efficiency ◽  
Lipopeptide Biosurfactant

A report on extensive lateral genetic reciprocation between arsenic resistant Bacillus subtilis and Bacillus pumilus strains analyzed using RAPD-PCR

2017 ◽  
Vol 107 ◽  
pp. 443-454 ◽  
Author(s):  
Sapna Khowal ◽  
Md. Zulquarnain Siddiqui ◽  
Shadab Ali ◽  
Mohd. Taha Khan ◽  
Mather Ali Khan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Pumilus ◽  
Rapd Pcr

scholarly journals Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Rury Eryna Putri ◽  
Nisa Rachmania Mubarik ◽  
Laksmi Ambarsari ◽  
Aris Tri Wahyudi
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Ammonium Sulfate ◽  
Enzyme Characterization ◽  
Antagonistic Activity ◽  
Phytopathogenic Fungus ◽  
Bacterial Isolates ◽  
Cell Wall Degrading Enzymes ◽  
Selection Medium

Abstract. Putri RE, Mubarik NR, Ambarsari L, Wahyudi AT. 2021. Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization. Biodiversitas 22: 4067-4077. Biocontrol of Fusarium oxysporum, a phytopathogenic fungus that causes plant wilt can be approached with cell-wall degrading enzymes such as ?-glucanase. The aim of this study was to evaluate the prospective ability in glucanase production from several soil bacterial isolates and to characterize its ?-glucanase activity of ammonium sulfate precipitation, and to determine its antifungal activity against F. oxysporum in vitro. Twenty bacterial isolates were screened qualitatively and quantitatively as ?-glucanase producers. The results showed that the prospective isolate W3.15 can produce ?-glucanase on glucan agar as the selection medium. From 16S rRNA sequences identification, the isolate belongs to the genus Bacillus, closely related to Bacillus subtilis. The enzyme activity of the ammonium sulfate fraction of isolate W3.15 is optimum at a pH of 7 and temperature range of 60-80oC. B. subtilis W3.15 exhibits high inhibition against the mycelial growth of F. oxysporum and significantly reduced fungal biomass.

scholarly journals Endophytic Bacteria Bacillus subtilis, Isolated from Zea mays, as Potential Biocontrol Agent against Botrytis cinerea

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 492
Author(s):  
Hernando José Bolivar-Anillo ◽  
Victoria E. González-Rodríguez ◽  
Jesús M. Cantoral ◽  
Darío García-Sánchez ◽  
Isidro G. Collado ◽  
...  
Keyword(s):  
Zea Mays ◽  
Bacillus Subtilis ◽  
Botrytis Cinerea ◽  
Endophytic Bacteria ◽  
Biochemical Properties ◽  
Plant Diseases ◽  
Phytopathogenic Fungus ◽  
Economic Losses

Plant diseases are one of the main factors responsible for food loss in the world, and 20–40% of such loss is caused by pathogenic infections. Botrytis cinerea is the most widely studied necrotrophic phytopathogenic fungus. It is responsible for incalculable economic losses due to the large number of host plants affected. Today, B. cinerea is controlled mainly by synthetic fungicides whose frequent application increases risk of resistance, thus making them unsustainable in terms of the environment and human health. In the search for new alternatives for the biocontrol of this pathogen, the use of endophytic microorganisms and their metabolites has gained momentum in recent years. In this work, we isolated endophytic bacteria from Zea mays cultivated in Colombia. Several strains of Bacillus subtilis, isolated and characterized in this work, exhibited growth inhibition against B. cinerea of more than 40% in in vitro cultures. These strains were characterized by studying several of their biochemical properties, such as production of lipopeptides, potassium solubilization, proteolytic and amylolytic capacity, production of siderophores, biofilm assays, and so on. We also analyzed: (i) its capacity to promote maize growth (Zea mays) in vivo, and (ii) its capacity to biocontrol B. cinerea during in vivo infection in plants (Phaseolus vulgaris).

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

Antibacterial activity of Celastrol against Bacillus subtilis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
N Padilla-Montaño ◽  
IL Bazzocchi ◽  
L Moujir
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis
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