scholarly journals Associations between Lactoferrin (LTF) gene polymorphism exon 4 and milk compositions in Senduro goat

2022 ◽  
Vol 335 ◽  
pp. 00043
Author(s):  
Tri Eko Susilorini ◽  
Aswah Ridhowi ◽  
Wike Andre Septian ◽  
Ahmad Furqon
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Exon 4 ◽  
Gene Exon

This research aimed to analyze the polymorphism of Lactoferrin (LTF) gene exon 4 associated with milk compositions in Senduro goats. A total of 42 DNA samples and milk compositions from Senduro goats were used in this study. The DNA sequence was amplified using Polymerase Chain Reaction (PCR) with a pair of primers. Genotyping was carried out using DNA sequencing and analyzed using FinchTV 1.4.0 and MEGA 6.0. In this research, the results showed that there were three genotypes (CC, CT, and TT) and two alleles (C and T). The frequencies of CC, CT, and TT genotypes were 0.381; 0.452; and 0.167, respectively. Furthermore, the frequencies of C and T alleles were 0.607 and 0.393, respectively. The genotype polymorphism did not affect on milk compositions. In conclusion, there was no association between polymorphism of LTF gene exon 4 and milk compositions in Senduro goats.

Automated DNA sequencing methods involving polymerase chain reaction.

1989 ◽  
Vol 35 (11) ◽  
pp. 2196-2201 ◽  
Author(s):  
L J McBride ◽  
S M Koepf ◽  
R A Gibbs ◽  
W Salser ◽  
P E Mayrand ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Sequence Data ◽  
Identification Accuracy ◽  
Universal Primers ◽  
Chain Reaction ◽  
Universal Primer ◽  
Polymerase Chain ◽  
Primer Sequence

Abstract Polymerase chain reaction (PCR) as a method for preparing DNA templates has been used for several DNA sequencing applications. An in situ procedure for directly sequencing PCR products by the dideoxy-termination method has been developed by using fluorophore-labeled sequencing primers. Completed sequence reactions were combined and loaded into a single electrophoretic lane of a fluorescence-based DNA sequence analyzer. DNA targets devoid of a universal primer sequence could be sequenced with labeled universal primers by incorporating a universal primer sequence into the PCR product. With this method, the sequence of a 351-bp region in the bacteriophage lambda genome was fully analyzed in a single lane with automatic base identification accuracy of greater than 99%. An unknown sequence, 1.7 kb long, also was sequenced by this procedure, in combination with a "PCR gene walking" strategy. Comparison of the 1110 bases in overlapping sequence data from both strands yielded only two single-base ambiguities. Automated DNA sequence analysis of the highly polymorphic HLA-DQA-1 (alpha) region in the human genome can be performed with this simple methodology. Use of this PCR-sequencing method to analyze DNA extracted from a one-month-old blood sample from an individual who is heterozygous at this locus allowed unambiguous assignment of genotype.

DNA sequence of the androgen receptor in prostatic tumor cell lines and tissue specimens assessed by means of the polymerase chain reaction

The Prostate ◽  
1993 ◽  
Vol 22 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Zoran Culig ◽  
Helmut Klocker ◽  
Johannes Eberle ◽  
Felizia Kaspar ◽  
Alfred Hobisch ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Androgen Receptor ◽  
Tumor Cell ◽  
Dna Sequence ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Prostatic Tumor ◽  
Tissue Specimens

Low frequency of CYP2A6 gene polymorphism as revealed by a one-step polymerase chain reaction method

1999 ◽  
Vol 9 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Gen-Fu Chen ◽  
Yong-Ming Tang ◽  
Bridgett Green ◽  
Dong-Xin Lin ◽  
F. Peter Guengerich ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Polymorphism ◽  
Low Frequency ◽  
Polymerase Chain Reaction Method ◽  
Chain Reaction ◽  
Reaction Method ◽  
One Step ◽  
Polymerase Chain ◽  
Cyp2a6 Gene ◽  
Step Polymerase Chain Reaction

Polymerase Chain Reaction Assay for Detection of Escherichia coli O157:H7 and Escherichia coli O157:H−

2002 ◽  
Vol 65 (1) ◽  
pp. 5-11 ◽  
Author(s):  
TAKAHISA MIYAMOTO ◽  
NATSUKO ICHIOKA ◽  
CHIE SASAKI ◽  
HIROSHI KOBAYASHI ◽  
KEN-ICHI HONJOH ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Dna Sequence ◽  
Escherichia Coli O157 ◽  
Chain Reaction ◽  
Pcr Assay ◽  
E Coli ◽  
Pcr Product ◽  
Genomic Dnas ◽  
Polymerase Chain

The DNA band patterns generated by polymerase chain reaction (PCR) using the du2 primer and template DNAs from various strains of Escherichia coli and non–E. coli bacteria were compared. Among three to five prominent bands produced, the three bands at about 1.8, 2.7, and 5.0 kb were detected in all of the E. coli O157 strains tested. Some nonpathogenic E. coli and all pathogenic E. coli except E. coli O157 showed bands at 1.8 and 5.0 kb. It seems that the band at 2.7 kb is specific to E. coli O157. Sequence analysis of the 2.7-kb PCR product revealed the presence of a DNA sequence specific to E. coli O157:H− and E. coli O157:H7. Since the DNA sequence from base 15 to base 1008 of the PCR product seems to be specific to E. coli O157, a PCR assay was carried out with various bacterial genomic DNAs and O157-FHC1 and O157-FHC2 primers that amplified the region between base 23 and base 994 of the 2.7-kb PCR product. A single band at 970 bp was clearly detected in all of the strains of E. coli O157:H− and E. coli O157:H7 tested. However, no band was amplified from template DNAs from other bacteria, including both nonpathogenic and pathogenic E. coli except E. coli O157. All raw meats inoculated with E. coli O157:H7 at 3 × 100 to 3.5 × 102 CFU/25 g were positive both for our PCR assay after cultivation in mEC-N broth at 42°C for 18 h and for the conventional cultural method.

Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats

2014 ◽  
Vol 54 (8) ◽  
pp. 987 ◽  
Author(s):  
M. Z. Fu ◽  
G. Li ◽  
Z. Q. Zhou
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Nucleotide Polymorphisms ◽  
Gene Polymorphism ◽  
Corpus Luteum ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Polymerase Chain

The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus, the minor allele frequency was 0.23. Associations of both SNPs with the number of embryos recovered and the corpus luteum number were evaluated in Boer and Shaanbei goat breeds. Association analysis showed that both SNPs had significant (P < 0.05) effects on the number of embryos recovered and corpus luteum number. These results indicate that LHβ and GnRHR genes are potential markers for the number of embryos recovered.

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