Cultivation of an Ovine Strain of Ehrlichia phagocytophila in Tick Cell Cultures

2002 ◽  
Vol 127 (2-3) ◽  
pp. 142-149 ◽  
Author(s):  
Z. Woldehiwet ◽  
B.K. Horrocks ◽  
H. Scaife ◽  
G. Ross ◽  
U.G. Munderloh ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Tick Cell ◽  
Ehrlichia Phagocytophila

scholarly journals Analysis on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two specific localities in Brazil

2021 ◽  
Vol 30 (3) ◽  
Author(s):  
Mayara de Cassia Luzzi ◽  
Lucas Amoroso Lopes de Carvalho ◽  
Daniel Guariz Pinheiro ◽  
Leidiane Lima-Duarte ◽  
Jaqueline Valéria Camargo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Rhipicephalus Sanguineus ◽  
Distribution Patterns ◽  
Embryonic Cell ◽  
Tick Cell ◽  
Illumina Platform ◽  
Region Gene ◽  
Host Environment ◽  
Canine Monocytic Ehrlichiosis ◽  
Vertebrate Hosts

Abstract Two lineages of Rhipicephalus sanguineus are known in Brazil: the temperate or southern and the tropical or northern populations. The distribution patterns of both lineages of R. sanguineus have epidemiological implications that can affect vectorial competence concerning Ehrlichia canis, the agent of canine monocytic ehrlichiosis. Intending to identify the microbiomes of both lineages and compare microorganisms in R. sanguineus, we used the 16S rRNA (V4-V5 region) gene-based metataxonomic approach, through NGS sequencing on the MiSeq Illumina platform. We selected specimens of females from the environment and samples of primary embryonic cell cultures, from both lineages, and this was the first study to investigate the prokaryotic microbiome in tick cell cultures. The results showed that many bacterial taxa detected in the samples were typical members of the host environment. A significant diversity of microorganisms in R. sanguineus females and in embryonic cell cultures from both lineages was found, with emphasis on the presence of Coxiella in all samples, albeit in different proportions. The Coxiella species present in the two lineages of ticks may be different and may have co-evolved with them, thus driving different patterns of interactions between ticks and the pathogens that they can harbor or transmit to vertebrate hosts.

scholarly journals Initiation of primary cell cultures from embryonic Haemaphysalis bispinosa ticks 

2017 ◽  
Vol 22 (3) ◽  
pp. 323 ◽  
Author(s):  
Fang-shiang Lim ◽  
Jing-jing Khoo ◽  
Fezshin Chen ◽  
Lesley Bell-sakyi ◽  
Chee-sieng Khor ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Embryonic Cell ◽  
Rrna Gene ◽  
Hard Tick ◽  
Tick Cell ◽  
Haemaphysalis Bispinosa ◽  
Ornithodoros Moubata ◽  
Floating Cell ◽  
Axenic Conditions

Tick cell cultures have been widely used as an important tool for the study of tick-associated microorganisms, specifically for medically important bacteria or viruses that may be difficult to isolate or culture in axenic conditions. In this study, primary embryonic tick cell cultures were initiated separately from each of the egg batches laid by 10 female ticks belonging to the hard tick genus Haemaphysalis. All cultures were maintained at 28°C. After 10 months, 4 healthy cultures were identified with the potential for developing into continuous tick cell lines. These cultures comprise large cells predominantly forming floating cell clumps with multicellular vesicles, which are morphologically similar to cell lines derived from the soft tick Ornithodoros moubata. Subculture has not yet been performed due to the low cell density at the time of writing. Amplification and sequencing of a fragment of the 16S rRNA gene from DNA extracted from the parent ticks showed 99%-100% similarity to published sequences of Haemaphysalis bispinosa. This is the first report of the initiation of embryonic cell cultures from Haemaphysalis ticks found in Malaysia. Such tick cell cultures will be useful for studies of tick-borne pathogens in this region, where recent studies have shown that Haemaphysalis ticks are highly represented and harbor medically important bacteria. 

Successful Infection of Tick Cell Cultures ofRhipicephalus sanguineus(Tropical Lineage) withEhrlichia canis

2018 ◽  
Vol 18 (12) ◽  
pp. 653-662
Author(s):  
Darci Moraes Barros-Battesti ◽  
Rosangela Zacarias Machado ◽  
Marcos Rogério André ◽  
Keyla Carstens Marques de Sousa ◽  
Daniella Aparecida Franze ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Tick Cell ◽  
Successful Infection

Ultrastructural analysis of the invasion of tick cells by Lyme disease spirochetes (Borrelia burgdorferi) in vitro

1994 ◽  
Vol 72 (6) ◽  
pp. 977-994 ◽  
Author(s):  
T. J. Kurtti ◽  
U. G. Munderloh ◽  
S. F. Hayes ◽  
D. E. Krueger ◽  
G. G. Ahlstrand
Keyword(s):  
Electron Microscopy ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Cell Lines ◽  
Cell Cultures ◽  
Dense Body ◽  
Tick Cell ◽  
Coated Pits ◽  
Intracellular Digestion

The association of Lyme disease spirochetes, Borrelia burgdorferi, with tick cell cultures was characterized by electron microscopy. These cells were active in endocytosis and intracellular digestion, containing coated vesicles, pits, and phagosomes. Borrelia burgdorferi in tick cell cultures resembled those described in tick tissues. In RAE25 cultures, isolated from Rhipicephalus appendiculatus embryos, invasion of cells was mediated by coated pits, indicating active host cell participation. The invading, tapered end of B. burgdorferi contained an electron-dense body that persisted throughout invasion. A host-derived membrane surrounded invading and intracellular borreliae as observed by transmission electron microscopy of cross and longitudinal sections, whereas degenerating ones lay inside secondary lysosomes. Borrelia burgdorferi cocultivated with cell lines from Ixodes scapularis were mainly found at the cell surface or within lysosomes. The differences seen in invasion of these cell lines are interpreted to reflect differences in cell types rather than species. Gemmae, indicative of degenerative changes in the spirochetes, were observed extra- and intra-cellularly. Membrane blebs were liberated by the spirochetes into the medium and onto the cells, and were avidly endocytosed. Tick cell cultures are a useful tool to elucidate spirochete – vector cell interactions that may be obscured in vivo.

scholarly journals Experimental Ixodes ricinus-Sheep Cycle of Anaplasma phagocytophilum NV2Os Propagated in Tick Cell Cultures

2020 ◽  
Vol 7 ◽  
Author(s):  
Consuelo Almazán ◽  
Lisa Fourniol ◽  
Clotilde Rouxel ◽  
Pilar Alberdi ◽  
Christelle Gandoin ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Tick Cell

Use of Percoll gradients to purify Anaplasma marginale (Rickettsiales: Anaplasmataceae) from tick cell cultures

2014 ◽  
Vol 5 (5) ◽  
pp. 511-515 ◽  
Author(s):  
Katarzyna Lis ◽  
Nour Najm ◽  
José de la Fuente ◽  
Isabel Fernández de Mera ◽  
Erich Zweygarth ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Anaplasma Marginale ◽  
Tick Cell ◽  
Percoll Gradients

scholarly journals Replication Kinetics of Rickettsia raoultii in Tick Cell Lines

2021 ◽  
Vol 9 (7) ◽  
pp. 1370
Author(s):  
Nurul Aini Husin ◽  
Jing Jing Khoo ◽  
Mulya Mustika Sari Zulkifli ◽  
Lesley Bell-Sakyi ◽  
Sazaly AbuBakar
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Growth Kinetics ◽  
Growth Curves ◽  
Growth Characteristics ◽  
Tick Cell ◽  
Rickettsia Raoultii ◽  
Generation Times ◽  
Causative Agents ◽  
Kinetics Of

Rickettsia raoultii is one of the causative agents of tick-borne lymphadenopathy in humans. This bacterium was previously isolated and propagated in tick cell lines; however, the growth characteristics have not been investigated. Here, we present the replication kinetics of R. raoultii in cell lines derived from different tick genera (BME/CTVM23, RSE/PILS35, and IDE8). Tick cell cultures were infected in duplicate with cryopreserved R. raoultii prepared from homologous cell lines. By 12–14 days post infection, 100% of the cells were infected, as visualized in Giemsa-stained cytocentrifuge smears. R. raoultii growth curves, determined by rickettsiae-specific gltA qPCR, exhibited lag, exponential, stationary and death phases. Exponential phases of 4–12 days and generation times of 0.9–2.6 days were observed. R. raoultii in BME/CTVM23 and RSE/PILS35 cultures showed, respectively, 39.5- and 37.1-fold increases compared to the inoculum. In contrast, multiplication of R. raoultii in the IDE8 cultures was 110.1-fold greater than the inoculum with a 7-day stationary phase. These findings suggest variation in the growth kinetics of R. raoultii in the different tick cell lines tested, amongst which IDE8 cells could tolerate the highest levels of R. raoultii replication. Further studies of R. raoultii are needed for a better understanding of its persistence within tick populations.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

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