P-L05 Enterohepatic migration of ingested fishbone resulting in hepatic abscess- Case report and review of literature

Background Ingestion of foreign bodies is common, particularly in children. In adults, most foreign body ingestions are related to food bolus or bone. The majority present with pharyngeal symptoms. Most foreign bodies exit the gastrointestinal tract without complication. However, around 1% result in perforation. Patients may have a delayed presentation with nonspecific symptoms and pose a diagnostic dilemma. We report a rare case of silent migration of a fishbone into the liver and a review of the literature. Methods We present the case of a 56 year old man who presented with a liver abscess second to an ingested fishbone. We conducted a PUBMED search and reviewed the published data over a period of thirty years. We identified 52 similar cases and compared the presentation, site of perforation and location of the fish bone. We observed the different approaches in presentation and management of such patients. Results A 56-year-old man presented to his local hospital with dull epigastric pain and raised inflammatory markers. CT scan revealed a 4 cm abscess in the left lobe of the liver, with a linear radio-dense body within. The patient was given antibiotics and the abscess was aspirated. The patient was transferred to our Hepatopancreaticobiliary unit for further management. Laparoscopy was performed. The left lateral segment of the liver was adherent to the gastric antrum. The hepato-gastric fistula was disconnected. The fishbone was retrieved from the liver. The abscess was drained and wash out performed. The patient was discharged the following day. Conclusions Left lobe liver abscess should raise suspicion of foreign body. Although antibiotic treatment may be effective in the short term, there is no long-term data regarding recurrence. We believe that laparoscopic drainage of the abscess and extraction of the foreign body offer control of the source of sepsis and reduces the risk of recurrence.

Prevalence of Some Common Human Traits: An Updated Survey Report From Rajshahi University Campus, Bangladesh

Prevalence of some common human traits viz., body weight, height and body mass index (BMI), pattern baldness, body hair, chin, colour blindness, cheek dimples, earlobes, length of index finger, lips, nose, polydactyly, tongue and widow’s peak, and ABO blood groups along with their Rh-D antigen, were recorded and analyzed from 500 male and 1000 female students of the Rajshahi University (RU) Campus. Results showed that body weights of the male students (66.42±8.92 kg) were significantly higher (t= 35.14; P<0.001) than those of the female students (51.64±6.97 kg). The differences in BMI between males (23.32±3.26) and females (20.85±2.63), as expected, were also highly significant (t= 15.79; P<0.001) and notably, some of the female students were underweight (BMI< 18.5). No female had baldness compared to 15.8% bald males. Sparse body hair was prevalent over the dense body hair in both genders. The females had much higher smooth chin (89.6%) than the males (58.0%) but the males had much higher cleft chin (42.0%) compared to the females (10.4%). No female was colourblind whereas 7.4% males were colourblind. Absence of dimples was recorded in 92.4% males and 85.7% females. Attached earlobes were higher than the free earlobes in both sexes. Compared to the length of the ring finger, shorter index finger was prevalent both in males (85.4%) and females (68.6%). Frequencies of the broad lips in males and females were 53.4% and 58.6%, respectively. Females dominated large and broad noses (76.1%) in comparison with their male counterparts (61.4%). Polydactyly was a rare trait in the Campus, where only 2.2% males and 0.2% females had extra digits in their hands or feet. Tongue rollers (53.8%) outnumbered the tongue folders in males, but tongue folders (54.4%) outnumbered the tongue rollers in females. Males with widow’s peak were higher (25.8%) than that in the females (19.0). Blood groups in the males and females were in the order: B (32.0) > O (29.8) > A (22.4) > AB (15.8) and O (34.8) > B (34.4) > A (21.9) > AB (8.9), respectively. As a whole, 85.4% males and 98.0% females were Rh-positive where the blood group phenotypes did not affect body weight, height and BMI of the subjects (r=0.012; P=0.63). The relevance of these findings to the physical, physiological, social and clinical well-being of the humans has been discussed. Bangladesh J. Zool. 49 (2): 215-228, 2021

Characteristics of economically important traits of cows depending on their body built

A comparative evaluation of Ukrainian Black-and-White Dairy cows of friable and dense, delicate and rough body built have been carried out. The dynamics of their live weight during the growing period, exterior, milk productivity and reproductive ability were investigated. Heifers with dense body built compared to the animals with friable body built were characterized by higher live weight at the age of 3; 6; 9; 12; 15 and 18 months and at the first insemination by 5,4-47,9 kg. Heifers with delicate body bult compared to the cows of the same age with rough body built were dominated by a live weight during growing period by 2,0-24,4 kg. The average daily gain of heifers with dense body built was 665 g (+93 g compared to friable body bullt, P <0,01), average daily gain of heifers with delicate body bult was 624 g (+24 g compared to rough body built). The primiparous cows with friable body built had larger sizes than cows of the same age with dense body built. At the withers they were higher by 1,2 cm, they had an advantage in chest girth – 8,9 cm (P < 0,001), chest width and chest depth behind the shoulder – 3,2 cm (P < 0,001) and 2,0 cm (P < 0,05), respectively, chest width and chest depth behind the last rib – 3,1 cm (P < 0,01) and 2,6 cm (P < 0,001), chest length – 2,1 cm (P < 0,05 ). The primiparous cows with delicate and rough body built were characterized by less marked differences in body measurements. Cows with delicate body bult compared to the cows with rough body built were taller by 4,9 cm (P < 0,001), with deeper chest behind the shoulder –1,7 cm (P < 0,05) and with longer body (oblique body length was more on 1,3 cm). Cows with rough body built had significantly larger chest width behind the last rib – by 2,1 cm (P < 0,05) and metacarpus girth – by 0,7 cm (P < 0,01). Higher milk productivity has been observed in cows with dense and delicate body built. The primiparous cows with dense body built had higher 305-d milk yield on 117 kg, milk fat yield –on 6,2 kg, milk protein yield – on 4,2 kg, fat content and protein content in milk – on 0,02% and 0,01%, respectively. Between cows with delicate and rough body built difference in milk yield was 340 kg, milk fat yield and milk protein – 12,1 kg and 10,5 kg, respectively,fat content and protein content in milk had no differences. Better reproductive ability has been observed in cows with friable body built (open days – 157, calf crop percent – 85,5%) and rough body built (145 days and 85,9%, respectively), it meant in cows with body built, that had lower milk productivity. Taking into account the characteristics of cows with different body built makes it possible to optimize the selection process in a particular herd and directing the selection in the desired direction. Key words: cows, Ukrainian Black-and-White Dairy breed, body built, live weight, body measurements, milk productivity, reproduction ability.

RNA polymerase II depletion from the inactive X chromosome territory is not mediated by physical compartmentalization.

Sub-nuclear compartmentalization has been proposed to play an important role in gene regulation by segregating active and inactive parts of the genome in distinct physical and biochemical environments, where transcription and epigenetic factors are either concentrated or depleted. The inactive X chromosome offers a paradigm for studying sub-nuclear compartmentalization. When the non-coding Xist RNA coats the X chromosome, it recruits repressors and chromatin factors that trigger gene silencing, and forms a dense body of heterochromatin from which the transcription machinery appears to be excluded. Phase separation has been proposed to be involved in X-chromosome inactivation (XCI) and might explain exclusion of the transcription machinery by preventing its diffusion into the Xist-coated territory. Here, using quantitative fluorescence microscopy and single particle tracking, we show that RNA polymerase II (RNAPII) freely accesses the Xist territory during initiation of XCI, and that its diffusion is not prevented by biophysical constraints. Instead, the apparent depletion of RNAPII is due to the loss of its chromatin bound fraction. These findings demonstrate that initial exclusion of RNA Pol2 from the inactive X is a consequence of its reduced binding rate at the chromatin and gene level, rather than the biophysical compartmentalization of the inactive X heterochromatin domain. The Xist silent compartment is thus a biochemical rather than a biophysical compartment, at least during initiation of XCI.

Numerical Analysis of a Flow over Spheres Embedded on a Flat Wall

This paper presents the results of numerical simulations of flow in a periodic channel with the walls covered in the central part by spherical elements that have the same overall surface areas but different radii. Two distributions of the sphere are considered, with the subsequent rows placed one after another or shifted. The computations are performed using the high-order code, whereas the solid elements are modelled with the help of the immersed boundary method. For selected cases, the results are validated by comparison with the solutions obtained using the ANSYS Fluent code on a very dense body-fitted mesh. It was found that the increase in the sphere diameter slows down the flow, which is attributed to the larger blockage of the channel cross-section caused by larger spheres and the occurrence of intense mixing (recirculation) between the spheres. The velocity profiles in the vicinity of the sphere are largely dependent on sphere diameter and rise when it increases. It was found that the distribution of the spheres plays an important role only when the spheres are large. In the part of the channel far from the sphere, the velocity profiles are significantly influenced by the sphere diameter but seem to be independent of the sphere distributions.

Platelet Electron Microscopy: Utilizing LEAN Methodology to Optimize Laboratory Workflow

Background Platelet electron microscopy (PEM) is the gold standard methodology for diagnosing storage pool disorder, defined as a paucity of delta granules, alpha granules, or both. PEM literature is limited with few published resources and without well-developed interlaboratory standardization for the preparation and examination of platelet samples. Methods Whole mount (WM) dense body (DB) counts for 300 pediatric cases were reviewed to determine whether counting fewer platelets could yield the same results. For 6 cases, DB average was determined on the day of WM preparation and on 2 consecutive days. Both WM and thin section (TS) preparations were examined for all cases. Results Employing LEAN methodology, an algorithm was developed to markedly decrease the number of platelets counted and still ensure accurate results. WMs decay with time; a statistically significant difference in DB counts was noted between day 0 and day 1 ( p < .1). Twelve of 300 cases required both WM and TS preparations for a complete diagnosis. Conclusion It is possible to maintain accuracy and decrease 100 platelet DB counts by >75%. WMs must be counted on the day they are prepared to avoid false paucity of DB secondary to sample decay. An accurate evaluation of platelet morphology requires both the WM and TS techniques.

A Stereolithographic Model-Based Dense Body Plan Generation Method to Construct a Ship Hydrodynamic Coefficients Database

A ship’s body plan is a vital data resource of ship hydrodynamics analysis, especially for time-domain simulations. Motivated by 3D printing technology, a novel dense body plan generation method is developed in this study. The slicing algorithm is adopted to generate dense 2D body plans from ship stereolithographic models. The dense body plan can be produced automatically under arbitrary rotational angles and slices. Moreover, a section redistribution algorithm is integrated to eliminate the non-uniform distribution features in sliced data inherited from the stereolithographic models. The benchmark ship models are selected to validate the accuracy of the method. The hull volumes of three ship models are calculated based on the produced data. The calculated results show satisfactory agreement with the published values. Furthermore, the estimation formulas of wetted surface area (WSA) are reviewed and utilized for validation. The calculated WSAs by slice integration turn out to be adaptive and accurate. The time costs of different slices are provided to illustrate the computational efficiency. A ship hydrodynamic coefficients database is constructed based on a 2D strip method and the produced data. The proposed method aims to improve the generation process of the body plan, which could meet the accuracy requirements of the strip method. As a result, hydrodynamic coefficients utilized in time-domain simulations could be obtained smoothly from the database.

Multiple Pulmonary Calssified Nodules and Cavitation Filled With the Dense Body on Chest X-Ray: A Case Report

Imaging in the clinical study has a significant value in medical diagnosing and the following diseases. The characteristic signs of imaging are a clue in approach to differential diagnosis. A conventional chest x-ray radiography is a more prevalent image used in the initial clinical assessment of pulmonary diseases. Iran is one of the 9 countries with a 95% burden of tuberculosis in the Eastern Mediterranian region. Chest radiography is the initial imaging for the evaluation of pulmonary tuberculosis.