scholarly journals Experimental Ixodes ricinus-Sheep Cycle of Anaplasma phagocytophilum NV2Os Propagated in Tick Cell Cultures

2020 ◽  
Vol 7 ◽  
Author(s):  
Consuelo Almazán ◽  
Lisa Fourniol ◽  
Clotilde Rouxel ◽  
Pilar Alberdi ◽  
Christelle Gandoin ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Tick Cell

scholarly journals Detection and prevalence of Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus ticks in seven study areas in Sweden

2010 ◽  
Vol 3 (1) ◽  
pp. 66 ◽  
Author(s):  
Kristofer Severinsson ◽  
Thomas G Jaenson ◽  
John Pettersson ◽  
Kerstin Falk ◽  
Kenneth Nilsson
Keyword(s):  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Rickettsia Helvetica ◽  
Ixodes Ricinus Ticks

scholarly journals Analysis on the prokaryotic microbiome in females and embryonic cell cultures of Rhipicephalus sanguineus tropical and temperate lineages from two specific localities in Brazil

2021 ◽  
Vol 30 (3) ◽  
Author(s):  
Mayara de Cassia Luzzi ◽  
Lucas Amoroso Lopes de Carvalho ◽  
Daniel Guariz Pinheiro ◽  
Leidiane Lima-Duarte ◽  
Jaqueline Valéria Camargo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Rhipicephalus Sanguineus ◽  
Distribution Patterns ◽  
Embryonic Cell ◽  
Tick Cell ◽  
Illumina Platform ◽  
Region Gene ◽  
Host Environment ◽  
Canine Monocytic Ehrlichiosis ◽  
Vertebrate Hosts

Abstract Two lineages of Rhipicephalus sanguineus are known in Brazil: the temperate or southern and the tropical or northern populations. The distribution patterns of both lineages of R. sanguineus have epidemiological implications that can affect vectorial competence concerning Ehrlichia canis, the agent of canine monocytic ehrlichiosis. Intending to identify the microbiomes of both lineages and compare microorganisms in R. sanguineus, we used the 16S rRNA (V4-V5 region) gene-based metataxonomic approach, through NGS sequencing on the MiSeq Illumina platform. We selected specimens of females from the environment and samples of primary embryonic cell cultures, from both lineages, and this was the first study to investigate the prokaryotic microbiome in tick cell cultures. The results showed that many bacterial taxa detected in the samples were typical members of the host environment. A significant diversity of microorganisms in R. sanguineus females and in embryonic cell cultures from both lineages was found, with emphasis on the presence of Coxiella in all samples, albeit in different proportions. The Coxiella species present in the two lineages of ticks may be different and may have co-evolved with them, thus driving different patterns of interactions between ticks and the pathogens that they can harbor or transmit to vertebrate hosts.

scholarly journals Akutni perikarditis u koinfekciji uzročnicima Anaplasma phagocytophilum i Borrelia burgdorferi

2021 ◽  
Vol 41 (1) ◽  
pp. 27-29
Author(s):  
Samira Knežević ◽  
Irena Slavuljica ◽  
Anamarija Flego Bojić ◽  
Đurđica Cekinović-Grbeša ◽  
Lari Gorup
Keyword(s):  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum

Lajmska borelioza i krpeljni meningoencefalitis (KME) najčešće su bolesti prenosive krpeljima u Republici Hrvatskoj. Isti vektor, krpelj roda Ixodes ricinus, odgovoran je i za prijenos humane granulocitne anaplazmoze (HGA) koja se u našoj zemlji rijetko dokazuje, a prvi slučajevi su potvrđeni 1998. godine u Koprivničko-križevačkoj županiji. HGA se najčešće klinički prezentira vrućicom s leukopenijom, trombocitopenijom, povišenim aminotransferazama i CRP-om, a potvrđuje se serološki, pri čemu se povremeno dokaže koinfekcija s virusom KME i/ili bakterijom Borrelia burgdorferi. Prikazujemo slučaj 44-godišnje bolesnice koja je ambulantno liječena u Klinici za infektivne bolesti Kliničkog bolničkog centra Rijeka zbog vrućice s bicitopenijom (leukopenija, trombocitopenija) i akutnog perikarditisa. Serološkom je obradom dokazana akutna koinfekcija uzročnicima Anaplasma phagocytophilum i Borrelia burgdorferi. Liječenje je provedeno doksiciklinom i nesteroidnim antireumaticima, čime je postignut povoljan klinički odgovor.

scholarly journals Relative Importance of Ixodes ricinus and Ixodes trianguliceps as Vectors for Anaplasma phagocytophilum and Babesia microti in Field Vole (Microtus agrestis) Populations

2008 ◽  
Vol 74 (23) ◽  
pp. 7118-7125 ◽  
Author(s):  
K. J. Bown ◽  
X. Lambin ◽  
G. R. Telford ◽  
N. H. Ogden ◽  
S. Telfer ◽  
...  
Keyword(s):  
United Kingdom ◽  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Babesia Microti ◽  
Infection Prevalence ◽  
Microtus Agrestis ◽  
Relative Importance ◽  
The United Kingdom ◽  
Ixodes Trianguliceps ◽  
Larval Burden

ABSTRACT The importance of Ixodes ricinus in the transmission of tick-borne pathogens is well recognized in the United Kingdom and across Europe. However, the role of coexisting Ixodes species, such as the widely distributed species Ixodes trianguliceps, as alternative vectors for these pathogens has received little attention. This study aimed to assess the relative importance of I. ricinus and I. trianguliceps in the transmission of Anaplasma phagocytophilum and Babesia microti among United Kingdom field voles (Microtus agrestis), which serve as reservoir hosts for both pathogens. While all instars of I. trianguliceps feed exclusively on small mammals, I. ricinus adults feed primarily on larger hosts such as deer. The abundance of both tick species and pathogen infection prevalence in field voles were monitored at sites surrounded with fencing that excluded deer and at sites where deer were free to roam. As expected, fencing significantly reduced the larval burden of I. ricinus on field voles and the abundance of questing nymphs, but the larval burden of I. trianguliceps was not significantly affected. The prevalence of A. phagocytophilum and B. microti infections was not significantly affected by the presence of fencing, suggesting that I. trianguliceps is their principal vector. The prevalence of nymphal and adult ticks on field voles was also unaffected, indicating that relatively few non-larval I. ricinus ticks feed upon field voles. This study provides compelling evidence for the importance of I. trianguliceps in maintaining these enzootic tick-borne infections, while highlighting the potential for such infections to escape into alternative hosts via I. ricinus.

Ixodes inopinatus in northern Germany: occurrence and potential vector role for Borrelia spp., Rickettsia spp., and Anaplasma phagocytophilum in comparison with Ixodes ricinus

2019 ◽  
Vol 118 (12) ◽  
pp. 3205-3216 ◽  
Author(s):  
Daniela Hauck ◽  
Andrea Springer ◽  
Stefan Pachnicke ◽  
Bettina Schunack ◽  
Volker Fingerle ◽  
...  
Keyword(s):  
Ixodes Ricinus ◽  
Anaplasma Phagocytophilum ◽  
Northern Germany ◽  
Potential Vector

scholarly journals Initiation of primary cell cultures from embryonic Haemaphysalis bispinosa ticks 

2017 ◽  
Vol 22 (3) ◽  
pp. 323 ◽  
Author(s):  
Fang-shiang Lim ◽  
Jing-jing Khoo ◽  
Fezshin Chen ◽  
Lesley Bell-sakyi ◽  
Chee-sieng Khor ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Embryonic Cell ◽  
Rrna Gene ◽  
Hard Tick ◽  
Tick Cell ◽  
Haemaphysalis Bispinosa ◽  
Ornithodoros Moubata ◽  
Floating Cell ◽  
Axenic Conditions

Tick cell cultures have been widely used as an important tool for the study of tick-associated microorganisms, specifically for medically important bacteria or viruses that may be difficult to isolate or culture in axenic conditions. In this study, primary embryonic tick cell cultures were initiated separately from each of the egg batches laid by 10 female ticks belonging to the hard tick genus Haemaphysalis. All cultures were maintained at 28°C. After 10 months, 4 healthy cultures were identified with the potential for developing into continuous tick cell lines. These cultures comprise large cells predominantly forming floating cell clumps with multicellular vesicles, which are morphologically similar to cell lines derived from the soft tick Ornithodoros moubata. Subculture has not yet been performed due to the low cell density at the time of writing. Amplification and sequencing of a fragment of the 16S rRNA gene from DNA extracted from the parent ticks showed 99%-100% similarity to published sequences of Haemaphysalis bispinosa. This is the first report of the initiation of embryonic cell cultures from Haemaphysalis ticks found in Malaysia. Such tick cell cultures will be useful for studies of tick-borne pathogens in this region, where recent studies have shown that Haemaphysalis ticks are highly represented and harbor medically important bacteria. 

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