First Evidence of a Trisegmented Double-Stranded RNA Virus in Canine Faeces

2001 ◽  
Vol 161 (2) ◽  
pp. 205-207 ◽  
Author(s):  
E.M VOLOTÄO ◽  
C.C SOARES ◽  
M.C.M ALBUQUERQUE ◽  
F.M.D.A SILVA ◽  
T.R.B CARVALHO ◽  
...  
Keyword(s):  
Rna Virus ◽  
Double Stranded Rna

scholarly journals N6-methyladenosine RNA modification suppresses antiviral innate sensing pathways via reshaping double-stranded RNA

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weinan Qiu ◽  
Qingyang Zhang ◽  
Rui Zhang ◽  
Yangxu Lu ◽  
Xin Wang ◽  
...  
Keyword(s):  
Rna Virus ◽  
Negative Regulator ◽  
Rna Modification ◽  
Type I ◽  
Viral Immunity ◽  
Potential Therapeutic Target ◽  
Viral Dsrna ◽  
Double Stranded Rna ◽  
Genetic Ablation ◽  
Vesicular Stomatitis

AbstractDouble-stranded RNA (dsRNA) is a virus-encoded signature capable of triggering intracellular Rig-like receptors (RLR) to activate antiviral signaling, but whether intercellular dsRNA structural reshaping mediated by the N6-methyladenosine (m6A) modification modulates this process remains largely unknown. Here, we show that, in response to infection by the RNA virus Vesicular Stomatitis Virus (VSV), the m6A methyltransferase METTL3 translocates into the cytoplasm to increase m6A modification on virus-derived transcripts and decrease viral dsRNA formation, thereby reducing virus-sensing efficacy by RLRs such as RIG-I and MDA5 and dampening antiviral immune signaling. Meanwhile, the genetic ablation of METTL3 in monocyte or hepatocyte causes enhanced type I IFN expression and accelerates VSV clearance. Our findings thus implicate METTL3-mediated m6A RNA modification on viral RNAs as a negative regulator for innate sensing pathways of dsRNA, and also hint METTL3 as a potential therapeutic target for the modulation of anti-viral immunity.

scholarly journals Cardiomyopathy Syndrome of Atlantic Salmon (Salmo salar L.) Is Caused by a Double-Stranded RNA Virus of the Totiviridae Family

2011 ◽  
Vol 85 (11) ◽  
pp. 5275-5286 ◽  
Author(s):  
O. Haugland ◽  
A. B. Mikalsen ◽  
P. Nilsen ◽  
K. Lindmo ◽  
B. J. Thu ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Rna Virus ◽  
Double Stranded Rna ◽  
Atlantic Salmon Salmo Salar

The coat protein of the yeast double-stranded RNA virus L-A attaches covalently to the cap structure of eukaryotic mRNA

1992 ◽  
Vol 12 (8) ◽  
pp. 3390-3398
Author(s):  
A Blanc ◽  
C Goyer ◽  
N Sonenberg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Coat Protein ◽  
Translation Initiation ◽  
Rna Virus ◽  
Binding Protein ◽  
Covalent Bond ◽  
Cellular Proteins ◽  
Double Stranded Rna ◽  
Virus Coat ◽  
Drosophila Cells

The eukaryotic mRNA 5' cap structure m7GpppX (where X is any nucleotide) interacts with a number of cellular proteins. Several of these proteins were studied in mammalian, yeast, and drosophila cells and found to be involved in translation initiation. Here we describe a novel cap-binding protein, the coat protein of L-A, a double-stranded RNA virus that is persistently maintained in many Saccharomyces cerevisiae strains. The results also suggest that the coat protein of a related double-stranded RNA virus (L-BC) is likewise a cap-binding protein. Strikingly, in contrast to the cellular cap-binding proteins, the interaction between the L-A virus coat protein and the cap structure is through a covalent bond.

scholarly journals Evidence of Intragenic Recombination in African Horse Sickness Virus

Viruses ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 654 ◽  
Author(s):  
Harry G. Ngoveni ◽  
Antoinette van Schalkwyk ◽  
J.J. Otto Koekemoer
Keyword(s):  
Bluetongue Virus ◽  
Type Species ◽  
Genetic Recombination ◽  
Rna Virus ◽  
Intragenic Recombination ◽  
African Horse Sickness ◽  
African Horse Sickness Virus ◽  
Double Stranded Rna ◽  
The Family ◽  
Attenuated Viruses

Intragenic recombination has been described in various RNA viruses as a mechanism to increase genetic diversity, resulting in increased virulence, expanded host range, or adaptability to a changing environment. Orbiviruses are no exception to this, with intragenic recombination previously detected in the type species, bluetongue virus (BTV). African horse sickness virus (AHSV) is a double-stranded RNA virus belonging to the Oribivirus genus in the family Reoviridae. Genetic recombination through reassortment has been described in AHSV, but not through homologous intragenic recombination. The influence of the latter on the evolution of AHSV was investigated by analyzing the complete genomes of more than 100 viruses to identify evidence of recombination. Segment-1, segment-6, segment-7, and segment-10 showed evidence of intragenic recombination, yet only one (Segment-10) of these events was manifested in subsequent lineages. The other three hybrid segments were as a result of recombination between field isolates and the vaccine derived live attenuated viruses (ALVs).

scholarly journals Double-stranded RNA virus outer shell assembly by bona fide domain-swapping

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Zhaoyang Sun ◽  
Kamel El Omari ◽  
Xiaoyu Sun ◽  
Serban L. Ilca ◽  
Abhay Kotecha ◽  
...  
Keyword(s):  
Rna Virus ◽  
Outer Shell ◽  
Domain Swapping ◽  
Double Stranded Rna ◽  
Bona Fide

scholarly journals Structural Polymorphism of the Major Capsid Protein of a Double-Stranded RNA Virus: An Amphipathic α Helix as a Molecular Switch

Structure ◽  
2005 ◽  
Vol 13 (7) ◽  
pp. 1007-1017 ◽  
Author(s):  
Irene Saugar ◽  
Daniel Luque ◽  
Ana Oña ◽  
José F. Rodríguez ◽  
José L. Carrascosa ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Major Capsid Protein ◽  
Rna Virus ◽  
Molecular Switch ◽  
Structural Polymorphism ◽  
Double Stranded Rna ◽  
Α Helix

scholarly journals Functions of Conserved Motifs in the RNA-Dependent RNA Polymerase of a Yeast Double-Stranded RNA Virus

1998 ◽  
Vol 72 (5) ◽  
pp. 4427-4429 ◽  
Author(s):  
Eric Routhier ◽  
Jeremy A. Bruenn
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Rna Virus ◽  
Rna Dependent Rna Polymerase ◽  
Conserved Motifs ◽  
Double Stranded Rna ◽  
Conserved Regions

ABSTRACT At least eight conserved motifs are visible in the totivirus RNA-dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiaedouble-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons.

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