scholarly journals What about Platelet Function in Platelet Concentrates?

2020 ◽  
Vol 40 (04) ◽  
pp. 500-508
Author(s):  
Michel Prudent
Keyword(s):  
Cell Biology ◽  
Ex Vivo ◽  
Control Process ◽  
Blood Component ◽  
Functional Markers ◽  
Transfusion Medicine ◽  
Platelet Concentrates ◽  
Quality Control Process ◽  
Basic Parameters ◽  
The Impact

AbstractThe characterization of platelet concentrates (PCs) in transfusion medicine has been performed with different analytical methods and platelet lesions (from biochemistry to cell biology) have been documented. In routine quality assessment and validation of manufacturing processes of PCs for transfusion purposes, only basic parameters are monitored and the platelet functions are not included. However, PCs undergo several manipulations during the processing and the basic parameters do not provide sensitive analyses to properly picture out the impact of the blood component preparation and storage on platelets. To improve the transfusion supply chain and the platelet functionalities, additional parameters should be used. The present short review will focus on the different techniques to monitor ex vivo platelet lesions from phenotype characterization to advanced omic analyses. Then, the opportunities to use these methods in quality control, process validation, development, and research will be discussed. Functional markers should be considered because they would be an advantage for the future developments in transfusion medicine.

Calculation of helical gear’s basic parameters using COP-data acquired by optical 3D digitizer

2011 ◽  
Vol 225 (12) ◽  
pp. 2953-2962 ◽  
Author(s):  
P Asgharifard-Sharabiani ◽  
A Hannaneh ◽  
M Nikkhah-Bahrami
Keyword(s):  
Gear Tooth ◽  
Control Process ◽  
Surface Fitting ◽  
Optical Data ◽  
Tooth Surface ◽  
Cylinder Surface ◽  
Quality Control Process ◽  
Basic Parameters ◽  
Cloud Of Points

Gears are industrial components with a precise geometry. Identification of their basic parameters plays an important role in their reverse design and quality control process. This article describes a new approach for the calculation of helical gear’s basic parameters using optical data acquired by 3D digitizer. This approach is implemented by acquiring cloud-of-points data (COP-data) from the bearing seats and gear tooth surface. Cylinder surface fitting through COP-data acquired from bearing seats is performed for the determination of gear axis of rotation. In a final step, involute helicoid surface fitting through COP-data acquired from gear tooth surface determines the helical gear’s primary features. Particle swarm optimization algorithm as an efficient method is applied to perform the surface fitting process in this article.

scholarly journals A divergent nonsense-mediated decay machinery in Plasmodium falciparum is inefficient and non-essential

2021 ◽  
Author(s):  
Emma McHugh ◽  
Michaela S. Bulloch ◽  
Steven Batinovic ◽  
Drishti K. Sarna ◽  
Stuart A. Ralph
Keyword(s):  
Plasmodium Falciparum ◽  
Mrna Decay ◽  
Ex Vivo ◽  
Intron Retention ◽  
Regulation Of Gene Expression ◽  
Control Process ◽  
Premature Termination Codon ◽  
Nonsense Mediated Decay ◽  
Quality Control Process ◽  
Mrna Binding

ABSTRACTNonsense-mediated decay (NMD) is a conserved mRNA quality control process that eliminates transcripts bearing a premature termination codon. In addition to its role in removing erroneous transcripts, NMD is involved in post-transcriptional regulation of gene expression via programmed intron retention in metazoans. The apicomplexan parasite Plasmodium falciparum shows relatively high levels of intron retention, but it is unclear whether these variant transcripts are functional targets of NMD. In this study, we use CRISPR-Cas9 to disrupt and epitope-tag two core NMD components: PfUPF1 (PF3D7_1005500) and PfUPF2 (PF3D7_0925800). Using RNA-seq, we find that NMD in P. falciparum is highly derived and requires UPF2, but not UPF1 for transcript degradation. Furthermore, our work suggests that the majority of intron retention in P. falciparum has no functional role and that NMD is not required for parasite growth ex vivo. We localise both PfUPF1 and PfUPF2 to puncta within the parasite cytoplasm, which may represent processing bodies - ribonucleoparticles that are sites of cytoplasmic mRNA decay. Finally, we identify a number of mRNA-binding proteins that co-immunoprecipitate with the NMD core complex and propose a model for a divergent NMD that does not require PfUPF1 and incorporates novel accessory proteins to elicit mRNA decay.

4-1BBL-Expressing aAPCs Attenuate IL-15-Induced NK Cell Expansion and Cytokine Production In Vitro but Induce NK Cell-Mediated Gvhd In Vivo

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 2536-2536
Author(s):  
Christian M. Capitini ◽  
Joanna L. Meadors ◽  
Monica M. Cho ◽  
Rimas J. Orentas ◽  
Crystal L. Mackall ◽  
...  
Keyword(s):  
Nk Cells ◽  
Cell Biology ◽  
Cytokine Production ◽  
Nk Cell ◽  
Conflicts Of Interest ◽  
Ex Vivo ◽  
Adoptive Cell Therapy ◽  
Receptor Expression ◽  
The Impact ◽  
Cells Cultured

Abstract Abstract 2536 Methods to expand natural killer (NK) cells ex vivo for adoptive cell therapy are being explored to improve outcomes after allogeneic blood and marrow transplant (alloBMT). Artificial antigen presenting cells (aAPCs) can present cytokines and/or co-stimulatory molecules that can potentially improve expansion and activity. 4-1BBL (CD137L) has demonstrated mixed results on murine and human NK cells, but the impact on murine NK cell biology after alloBMT has not been explored. NK cells were harvested from either C57BL/6 (B6) or CB6F1 spleens and cultured ex vivo with a recombinant interleukin (IL)-15/IL-15 receptor alpha (Ra) complex in the presence or absence of a CD137L+ aAPC. Because IL-15 is typically presented in trans by IL-15Ra, the complex was utilized to potently increase agonist bioactivity. NK cells cultured with IL-15/IL-15Ra alone showed a peak of 20-fold expansion, but this expansion was decreased with the addition of CD137L+ aAPCs if the ratio of aAPC to NK cells was greater than 1:1. In the presence of IL-15/IL-15Ra, the impact of CD137L+ aAPCs on expression of the inhibitory receptors, Ly49C+I and activating receptor Ly49H was variable and strain dependent, with increased expression in B6 NK cells, but decreased expression in CB6F1 NK cells. The expression of major histocompatibility complex (MHC) class I was not affected in NK cells from either strain by the presence of CD137L+ aAPCs. The production of gamma interferon and tumor necrosis factor-a was robust in NK cells expanded by IL-15/IL-15Ra alone, but attenuated with the addition of CD137L+ aAPCs. Animal experiments showed that administration of NK cells expanded ex vivo with IL-15/IL-15Ra alone was well tolerated after T cell depleted MHC-mismatched alloBMT (CB6F1–>B6), but surprisingly the addition of CD137L+ aAPCs to cultures caused NK cells to induce GVHD-associated weight loss. In summary, IL-15/IL-15Ra expanded murine NK cells demonstrate increased cytokine production and do not cause toxicity when infused after alloBMT. The presence of CD137L+ aAPCs attenuated cytokine production and increased Ly49 receptor expression in NK cells from B6 mice. Remarkably, NK cells expanded by IL-15/IL-15Ra in the presence of CD137L+ aAPCs demonstrate increased propensity to cause GVHD. Ongoing studies are exploring the anti-tumor efficacy of IL-15/IL-15Ra expanded murine NK cells cultured in the presence and absence of CD137L. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Analysis of the Successful Implementation of Tazi's Important Innovations in Tackling Stunting in the Work Area of the Rusip Antara Public Health Center

2021 ◽  
Vol 2 (4) ◽  
pp. 114-127
Author(s):  
Maulida Maulida ◽  
Thomson P. Nadapdap ◽  
Zuraidah Nasution
Keyword(s):  
Health Center ◽  
Control Process ◽  
Secondary Data ◽  
Primary Data ◽  
Successful Implementation ◽  
Nutrition Interventions ◽  
Work Area ◽  
Quality Control Process ◽  
Nutrition Intake ◽  
The Impact

The research objectives were to analyze the successful implementation of Tazi's Important Innovations in Strategy, Interventions, increasing the scope of interventions for the target of 1000 HPK households, improving nutrition intake and reducing infections and the impact of Tazi's important innovations in preventing stunting in the working area of ​​the Rusip Health Center. The type and design of the research is descriptive qualitative. Data sources consist of Primary Data and Secondary Data. Data collection techniques consist of interviews, observations and documentation, data analysis techniques in the form of data reduction, data presentation, and drawing conclusions. Testing the validity of the data using source triangulation and member check. Quality control process is carried out by applying PDCA. Research ethics such as Informed consent, Anominity and confidentiality. The Regent of Central Aceh has made Regulation Number 14 of 2019 concerning stunting handling in Central Aceh Regency and is very committed to the prevention and reduction of stunting by implementing a stunting reduction strategy through 5 pillars, specific nutrition interventions and sensitive nutrition carried out in an integrated and converged manner with a target of 1000 HPK . The impact of Tazi's Important innovation is able to reduce the prevalence of stunting in Tirmiara Village. Implementation of the Strategy for the Acceleration of Stunting Prevention is based on five main pillars. Interventions to accelerate stunting prevention consist of specific and sensitive interventions. increasing the scope of intervention in the target of 1000 HPK households

Transfusion Medicine's Role in Hospital Performance Improvement: An Administrator's View

1999 ◽  
Vol 123 (6) ◽  
pp. 486-491
Author(s):  
George Krempel ◽  
Colleen Jarosz
Keyword(s):  
Performance Improvement ◽  
Inventory Management ◽  
Program Implementation ◽  
Hospital Performance ◽  
Blood Component ◽  
Transfusion Medicine ◽  
Blood Products ◽  
Hospital Administrators ◽  
The Impact

Abstract Historically, hospital administrators have viewed all laboratory sections as cost centers. The major expenses in the transfusion service are those associated with labor and blood products. However, few administrators take the time to look past this cost to see the impact of an active transfusion medicine section in other areas of the hospital. This article examines the impact of inventory management, blood component utilization and waste, group purchasing, and new program implementation on transfusion service expense and revenue.

Process management, innovation and efficiency performance

2014 ◽  
Vol 20 (2) ◽  
pp. 335-358 ◽  
Author(s):  
Janine L. Sanders Jones ◽  
Kevin Linderman
Keyword(s):  
Process Design ◽  
Process Management ◽  
Control Process ◽  
Innovation Performance ◽  
Statistical Quality Control ◽  
Competitive Intensity ◽  
Content Type ◽  
Quality Control Process ◽  
Functional Teams ◽  
The Impact

Purpose – Much of the practitioner literature touts the universal benefits of process management and its impact on operational performance. However, in academic literature, empirical evidence is mixed. The purpose of this study is to investigate the role of the competitive intensity on the effectiveness of process management. Design/methodology/approach – Survey data from manufacturing plants were collected from through a global research project. Regression analysis was used to test hypotheses. Findings – The influence of process design on efficiency and innovation performance is not dependent on competitive intensity; however, the impact of process improvement and process control on efficiency and innovation performance is in some instances moderated by competitive intensity. Research limitations/implications – The inclusion of competitive intensity as a contingency variable helps to explain the contextual impact of process management on efficiency and innovation. Practical implications – Process management can be an effective tool if the levels of process design, control, and improvement are customized to fit with the competitive environment. Originality/value – This is one of the few studies to empirically examine process management as three core elements. Previous studies utilized a single construct of process management or multiple manufacturing practices such as customer/supplier involvement, statistical quality control, process focus, and cross-functional teams to measure process management. Using this measurement approach demonstrates how process management can influence both efficiency and innovation.

scholarly journals Mitophagy: A New Player in Stem Cell Biology

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 481
Author(s):  
George Cairns ◽  
Madhavee Thumiah-Mootoo ◽  
Yan Burelle ◽  
Mireille Khacho
Keyword(s):  
Quality Control ◽  
Stem Cell ◽  
Cell Biology ◽  
Control Process ◽  
Ros Generation ◽  
Stem Cell Biology ◽  
Regulatory Pathways ◽  
Cell Functions ◽  
Quality Control Process ◽  
Quality Control Mechanism

The fundamental importance of functional mitochondria in the survival of most eukaryotic cells, through regulation of bioenergetics, cell death, calcium dynamics and reactive oxygen species (ROS) generation, is undisputed. However, with new avenues of research in stem cell biology these organelles have now emerged as signaling entities, actively involved in many aspects of stem cell functions, including self-renewal, commitment and differentiation. With this recent knowledge, it becomes evident that regulatory pathways that would ensure the maintenance of mitochondria with state-specific characteristics and the selective removal of organelles with sub-optimal functions must play a pivotal role in stem cells. As such, mitophagy, as an essential mitochondrial quality control mechanism, is beginning to gain appreciation within the stem cell field. Here we review and discuss recent advances in our knowledge pertaining to the roles of mitophagy in stem cell functions and the potential contributions of this specific quality control process on to the progression of aging and diseases.

Solow economy model

2019 ◽  
pp. 150-157
Author(s):  
Oleksandr Synenko ◽  
Kateryna Yarema ◽  
Yuliia Bezsmertna
Keyword(s):  
Economic Growth ◽  
Regression Analysis ◽  
Solow Model ◽  
Economy Model ◽  
Accelerated Depreciation ◽  
Basic Parameters ◽  
The Subject ◽  
Investment Process ◽  
The Impact ◽  
Model Algorithm

The subject of the research is the approach to the possibility of using the Solow model to perform the regression analysis on the example of the Ukrainian economy model. The purpose of writing this article is to investigate the notion of regres- sion analysis, Solow’s economy model, algorithm for performing regression analy- sis on the example of Ukraine’s economy model. This model can be adapted for the economy of enterprises. Methodology. The research methodology is system-struc- tural and comparative analyzes (to study the structure of GDP); monograph (when studying methods of regression analysis on the example of the Ukrainian economy); economic analysis (when assessing the impact of factors on Ukraine’s GDP). The scientific novelty consists the features of the use of the Solow model on the ex- ample of Ukrainian economy are determined. An algorithm for calculating the basic parameters of a model using the Excel application package is disclosed. The main recommendations on the development of the national economy and economic growth through the use of macroeconomic instruments are given. Conclusions. The use of the Solow model enables forecasting and analysis. The results obtained re- vealed the problem of low resource return of capital as a resource, along with the means of macroeconomic regulation of the investment process, using which can improve the situation. A special place in these funds belongs to the accelerated depreciation and interest rate policies.

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