Secondary metabolites and cytotoxic activity of the stem bark of Litsea akoensis

Objective: The present study deals with the cytotoxic activity of n-hexane and ethyl acetate extracts of Calotropis gigantea L. stem bark and its fractions such as A, B, C, D and E fractions on murine leukemia cell line P388.Methods: The crude extracts of C. gigantea stem bark were prepared using n-hexane and ethyl acetate solvents. The plant extracts were subjected to vacuum liquid chromatography followed by TLC. According to the similarity of stain patterns, the fractions were combined. The extracts and its combined fractions were then subjected for the phytochemical test. Cytotoxic activity of those extracts and its combined fractions were tested using MTT assay. Fraction D was subjected to gravity column chromatography followed by TLC. Then, fractions A, B, and D2 were crystallized and subjected to GC-MS.Results: The qualitative screening of n-hexane extract of Calotropis gigantea L. stem bark for secondary metabolites showed the presence of terpenoid, flavonoids, phenolics and coumarins. While the ethyl acetate extract contained phenolics, steroids, flavonoids, saponins and coumarins compounds. IC50 values for n-hexane extract and E fraction are 76.29 µg/ml and 18.48 µg/ml, respectively. In the ethyl acetate extract and C fraction obtained IC50 values 57.05 µg/ml and 52.58 µg/ml.Conclusion: Cytotoxic activity from E fraction of n-hexane extract of C. gigantea stem bark is the most potent and containing flavonoids, phenolics and coumarins. The main components from several compounds of n-hexane extract of C. gigantea are germacrane-A, (-)-globulol, urs-12-ene and veridiflorol.

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Secondary metabolites from Tetracera potatoria stem bark with anti-mycobacterial activity

Journal of Ethnopharmacology ◽

10.1016/j.jep.2016.11.027 ◽

2017 ◽

Vol 195 ◽

pp. 238-245 ◽

Cited By ~ 10

Author(s):

M.C.Y. Fomogne-Fodjo ◽

D.T. Ndinteh ◽

D.K. Olivier ◽

P. Kempgens ◽

S. van Vuuren ◽

...

Keyword(s):

Secondary Metabolites ◽

Stem Bark

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Phytochemical Screening and Biocidal Studies Of Stem Bark Chrysophyllum Albidum (Linn) And Straw Aristolochia Ringens from Nigeria.

JOURNAL OF RESEARCH AND REVIEW IN SCIENCE ◽

10.36108/jrrslasu/7102/40(0142) ◽

2017 ◽

Vol 4 (1) ◽

Author(s):

Moses Owolabi

Keyword(s):

Medicinal Plants ◽

Cytotoxic Activity ◽

Biological Activities ◽

Stem Bark ◽

Breast Tumour ◽

Tumour Cells ◽

Bacterial Activity ◽

Liver Tumour ◽

Phytochemical Screening ◽

Health Maintenance

Introduction: Medicinal plants play a significant role in the health maintenance in underdeveloped countries, herbs and spices continue serve as a new sources for herbal medicines. Aim: The objective was to investigate phytochemical screening and biocidal activity on stem bark Chrysophyllum albidum (Linn) and straw Aristolochia ringens using chloroform. Methods: Chloroform extracts of C. albidum and A. ringens were obtained and screened for phytochemical constituents. The colour intensity or the precipitate formation was used as analytical responses to these tests. The Antimicrobial activities crude extracts were evaluated against some microorganisms with broth microdilution test. The extracts were screened for in-vitro cytotoxic activity against breast tumour and liver tumour cells. Results: The results of the phytochemical screening revealed the presence of alkaloids, saponins, steroids, anthraquinone and glycosides. C. albidum and A. ringens chloroform extracts revealed high quantities of alkaloid and flavonoid respectively. While cardiac glycoside and anthraquinone were found with low quantity in C. albidum and A. ringens extracts respectively. C. albidum showed promising bacterial activity against Escherichia coli and cytotoxic activity to liver tumour cells. While A. ringens, showed excellent bacterial activity against Bacillus cereus and good fungal activity against Candida albicans. A. ringens also showed cytotoxic to liver tumour and breast tumour cells. The observed antimicrobial and cytotoxic effects may be due to the antagonist or synergistic effect of the secondary metabolites identified in the extracts. Conclusion: Medicinal plants studied showed good potential biological activities that support the idea that traditional medicines remain useful healthcare in developing countries

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Pentacyclic Triterpenes and Other Constituents from Ficus cordata (Moraceae)

Zeitschrift für Naturforschung B ◽

10.1515/znb-2008-1113 ◽

2008 ◽

Vol 63 (11) ◽

pp. 1335-1338 ◽

Cited By ~ 9

Author(s):

Herve M. P. Poumale ◽

Rodrigue T. Kengap ◽

Jean Claude Tchouankeu ◽

Felix Keumedjio ◽

Hartmut Laatsch ◽

...

Keyword(s):

Antifungal Activity ◽

Secondary Metabolites ◽

Betulinic Acid ◽

Nmr Spectra ◽

2D Nmr ◽

Stem Bark ◽

Published Data ◽

Pentacyclic Triterpenes ◽

2D Nmr Spectra ◽

Lupeol Acetate

Abstract Two new pentacyclic triterpenes 8,26-cyclo-urs-21-en-3β, 20β-diol (1) and 3β-acetoxy-8.26- cyclo-ursan-20β-ol (2) together with 3-friedelanone, oleanolic acid, betulinic acid, lupeol acetate, α- and β-amyrine, S.SJ^'-tetrahydroxyflavane, and 3,5,7,3',4'-pentahydroxyflavane were isolated from the stem bark of Ficus cordata (Moraceae). The structures of these secondary metabolites were established using ID and 2D NMR spectra and by comparison with published data or with authentic samples. Compounds 1 and 2 exhibited weak antibacterial and no antifungal activity.

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Senegalin, a new phenylpropanoid and other secondary metabolites from the stem bark of Ekebergia senegalensis A. Juss. (Meliaceae)

Natural Product Research ◽

10.1080/14786419.2020.1729151 ◽

2020 ◽

pp. 1-7

Author(s):

Guy Paulin M. Kemayou ◽

Gervais M. Happi ◽

Yvan Anderson T. Ngandjui ◽

Jean Claude Tchouankeu ◽

Norbert Sewald ◽

...

Keyword(s):

Secondary Metabolites ◽

Stem Bark

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Cytotoxic Activity of Compounds from Styrax obassia

Natural Product Communications ◽

10.1177/1934578x1701200230 ◽

2017 ◽

Vol 12 (2) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Thao Quyen Cao ◽

Bo Mi Lee ◽

Yeon Woo Jung ◽

Van Thu Nguyen ◽

Jeong Ah Kim ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Line ◽

Cancer Cell ◽

Leukemia Cell ◽

Cancer Cell Line ◽

Stem Bark ◽

Leukemia Cell Line ◽

Cervical Cancer Cell Line ◽

Cell Line Hela ◽

Mcf 7

Cancer is a major public health burden in both developed and developing countries. Plant-derived compounds have played an important role in the development of useful anti-cancer agents. The current study was designed to evaluate the cytotoxic activity of chemical compounds from the stem bark of Styrax obassia. Seven known compounds (1–7) were isolated and identified. Compound 2 exhibited cytotoxic activity against the breast cancer cell line MCF-7 with an IC50 of 27.9 μM, followed by the human cervical cancer cell line Hela with an IC50 of 23.3 μM, and the human promyelocytic leukemia cell line HL-60 with an IC50 of 47.8 μM. Compound 7 exhibited cytotoxicity against Hela cells with an IC50 of 16.8 μM, followed by MCF-7 cells with an IC50 of 53.5 μM. This is the first study to investigate the significant anti-tumor properties of isolated compounds from the stem bark of S. obassia.

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