Total Phenolics And Flavonoids Content, And Free Radical Scavenging Potential Of Tissue Culture Raised Stevia Rebaudiana Bertoni

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
H Lata ◽  
S Chandra ◽  
B Avula ◽  
S Khan ◽  
S Sagi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Free Radical ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
Free Radical Scavenging ◽  
Stevia Rebaudiana Bertoni

scholarly journals IN VITRO SCREENING AND CORRELATION OF ETHANOLIC PEEL EXTRACTS OF PUNICA GRANATUM AND CITRUS SINENSIS WITH RESPECT TO ANTIOXIDANT AND ANTIRADICAL ACTIVITY

2020 ◽  
pp. 52-56
Author(s):  
AJIT V SONTAKKE ◽  
ANUP S HENDRE ◽  
SANGITA R PATIL
Keyword(s):  
Free Radical ◽  
Citrus Sinensis ◽  
Antiradical Activity ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Free Radical Scavenging ◽  
Total Antioxidant ◽  
Free Radical Scavenging Assay

Objective: The objective of this study is to evaluate the effect of the ethanolic peel extracts of Punica granatum and Citrus sinensis on total phenolics, total antioxidants, and radical scavenging activities using different in vitro models. Methods: Pomegranate and orange fruits at the maturity stage were manually peeled, washed, and air dried before extraction. Dried peels were grinded, weighed, and extracted with 95% ethanol and were used for in vitro analysis. Different concentrations of extracts for each peel were prepared. Total phenolics content, total antioxidant capacity, and free radical scavenging activities of each peel extract were estimated in the form of Folin–Ciocalteu reagent assay, phosphomolybdenum assay, hydroxyl free radical scavenging assay, and superoxide free radical scavenging assay, respectively. Results: The ethanolic peel extracts of P. granatum and C. sinensis showed significant increased total phenolic contents and total antioxidant activity. Furthermore, radical scavenging activity (hydroxyl and superoxide) was found to be significant in both ethanolic peel extracts. Conclusion: It can be concluded from the study that the ethanolic peel extracts of P. granatum and C. sinensis has potential antioxidant and antiradical activity.

scholarly journals Optimization of Ultrasonic-Assisted Extraction of Total Phenolics from Citrus aurantium L. Blossoms and Evaluation of Free Radical Scavenging, Anti-HMG-CoA Reductase Activities

2019 ◽  
Author(s):  
Kexin Hao ◽  
Wenzhong Hu ◽  
Mengyang Hou ◽  
Duo Cao ◽  
Yu Wang ◽  
...  
Keyword(s):  
Free Radical ◽  
Total Phenolics ◽  
Reductase Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Citrus Aurantium ◽  
Hmg Coa Reductase ◽  
Ultrasonic Assisted ◽  
Coa Reductase

The objective of this study was to develop an ultrasonic-assisted procedure for the extraction of total phenolics from Citrus aurantium L. blossoms (CAB) and evaluate the free radical scavenging activity, anti-HMG-CoA reductase activity of total phenolics. In this work, a Box-Behnken design based on the single-factor experiments was used to explore the optimum extraction process. Under the optimum conditions (extraction solvent 70.31% ethanol, extraction temperature 61.94 °C, extraction time 51.73 min and liquid-to-solid ratio 35.63 mL/g), the extraction yield of total phenolics was 95.84 mg gallic acid equivalents (GAE)/g dry matter (DM), which was highly consistent with the theoretical value (96.12 mg GAE/g DM). The total phenolic extract showed excellent free radical scavenging properties against DPPH·, ABTS+·, ·OH and ·O2-, with the IC50 values of 197.007, 83.878, 218.643 and 158.885 μg/mL, respectively, and the extracts also showed good inhibition of HMG-CoA reductase activity, with the IC50 value of 117.165 μg/mL. Total phenolics from CAB could be a potential source of natural free radical scavenger and HMG-CoA reductase inhibitor.

scholarly journals Antioxidant Potential, Phenolics Content and Antimicrobial Attributes of Selected Medicinal Plant

2021 ◽  
Vol 22 (2) ◽  
pp. 307-319
Author(s):  
Ali Abbas ◽  
Bushra Sultana ◽  
Anwaar Hussain ◽  
Farooq Anwar ◽  
Naveed Ahmad
Keyword(s):  
Linoleic Acid ◽  
Free Radical ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Flavonoids ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Ethanol Extracts

The bioactive extracts recovered from Curcuma longa (stem), Mentha aquatica (stem and leaves), Emblica officinalis (fruits), Nigella sativa (seeds), and Glycyrrhiza glabra (stem) using methanol and ethanol, were appraised for antioxidant (total flavonoid contents, total phenolic contents, DPPH free radical scavenging ability, inhibition of linoleic acid peroxidation and reducing power) and antimicrobial attributes. Total phenolics (16.89 ± 0.18 - 25.06 ± 0.31g GAE/100g) and total flavonoids (1.96 ± 0.07 - 13.54 ± 0.18 CE g/100g) contents of aqueous methanol extracts of tested plant materials were significantly (p < 0.05) lower than that of aqueous ethanol extracts (13.87 ± 0.12 - 28.63 ± 0.34 g GAE/100g) and (2.20 ± 0.10 - 8.71 ± 0.24 CE /100g), respectively. The percent inhibition of linoleic acid per oxidation by crude ethanol extracts of plants was in the range of 48.72 ± 1.24 - 70.79 ± 1.57 and crude methanol extract 35.90 ± 1.28 - 61.54 ± 2.14, while the range of DPPH free radical scavenging activity of ethanol extracts was (58.36 ± 1.98 - 80.55 ± 3.07) and methanol extracts (39.55 ± 1.52 - 79.64± 2.33). The reducing power of the tested extracts obtained by ethanol (at the concentration of 10 mg/mL) ranged 1.11 ± 0.12 – 1.53 ± 0.18 while for methanol extracts 0.98 ± 0.11 – 1.39 ± 0.12. Among the attributes analyzed, total phenolics, total flavonoids, and DPPH free scavenging activity were found to be varied significantly in all the tested medicinal plants. The extracts from all plants showed good antimicrobial potential against a penal of bacteria, including E. coli, P. multocida and S. aureus, and fungi including A. niger, A. flavus, A. alternate, and G. lucidium. Overall, the tested samples were found to be a good source of natural antioxidant and antimicrobial agents and thus can be explored for potential functional food and nutra-pharmaceutical applications.

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