The Recovery of Factor VIII from Fresh-Frozen, Indated and Outdated Human Plasma

1976 ◽  
Vol 36 (01) ◽  
pp. 071-077 ◽  
Author(s):  
Daniel E. Whitman ◽  
Mary Ellen Switzer ◽  
Patrick A. McKee
Keyword(s):  
Factor Viii ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
The United States ◽  
Fresh Frozen Plasma ◽  
Red Cross ◽  
Random Samples ◽  
Fresh Frozen ◽  
Factor Viii Concentrates ◽  
Frozen Plasma

SummaryThe availability of factor VIII concentrates is frequently a limitation in the management of classical hemophilia. Such concentrates are prepared from fresh or fresh-frozen plasma. A significant volume of plasma in the United States becomes “indated”, i. e., in contact with red blood cells for 24 hours at 4°, and is therefore not used to prepare factor VIII concentrates. To evaluate this possible resource, partially purified factor VIII was prepared from random samples of fresh-frozen, indated and outdated plasma. The yield of factor VIII protein and procoagulant activity from indated plasma was about the same as that from fresh-frozen plasma. The yield from outdated plasma was substantially less. After further purification, factor VIII from the three sources gave a single subunit band when reduced and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These results indicate that the approximately 287,000 liters of indated plasma processed annually by the American National Red Cross (ANRC) could be used to prepare factor VIII concentrates of good quality. This resource alone could quadruple the supply of factor VIII available for therapy.

scholarly journals Successful Pregnancy in a Patient with Combined Deficiency of Factor V and Factor VIII

2014 ◽  
Vol 2014 ◽  
pp. 1-3
Author(s):  
Ahmed Ghassan El Adib ◽  
Farah Majdi ◽  
Mohamed Othmane Dilai ◽  
Hamid Asmouki ◽  
Ahlam Bassir ◽  
...  
Keyword(s):  
Factor Viii ◽  
Fetal Monitoring ◽  
Fresh Frozen Plasma ◽  
Factor V ◽  
Successful Pregnancy ◽  
Fresh Frozen ◽  
Factor Viii Concentrates ◽  
Low Levels ◽  
Frozen Plasma ◽  
Combined Deficiency

Inherited combined factor V and factor VIII deficiency (F5F8D) is autosomal recessive transmission disorder. Epistaxis, postsurgical bleeding, and menorrhagia are the most common symptoms. The risk of miscarriage and placental abruption is consequent. We report a case of successful pregnancy in a patient with F5F8D. 20-year-old woman, born of consanguineous parents, third gestate, first parity, two miscarriages, admitted for child birth of a spontaneous pregnancy estimated at 38 weeks and was diagnosed with F5F8D. At admission, patient was hemodynamically stable, with good obstetric conditions. The biologic results showed low levels of PT (52%), factor V (7%), and factor VIII (5%), and the activated partial thromboplastin time was prolonged (68,6%). Parturient was admitted in intensive care unit, maternal and fetal monitoring was performed. Fresh frozen plasma (FFP) and factor VIII concentrates were perfused at the induction of labor. Analgesia used fentanyl titration. The delivery gave birth to a newborn male, with Apgar 10/10 and 3000 g. The puerperium was simple without any important bleeding. Laboratory tests for the newborn were acceptable. Little literature is available on this subject and there are no guidelines available concerning pregnancy; we chose to prescribe a combination of factor VIII concentrate and FFP in pre-, per- and postpartum. The same protocol was successfully used in a patient before dental extraction and prostatectomy. Vaginal delivery is possible, as our case. Management by multidisciplinary team is recommended.

Leukemia and Hemophilia

PEDIATRICS ◽  
1986 ◽  
Vol 78 (3) ◽  
pp. 544-544
Author(s):  
SINASI OZSOYLU
Keyword(s):  
Factor Viii ◽  
Hemophilia A ◽  
Fresh Frozen Plasma ◽  
Fresh Frozen ◽  
Viii And Ix ◽  
Immune Changes ◽  
Frozen Plasma

To the Editor.— I enjoyed reading the paper by Aronis et al,1 and would like to bring to your attention that we have also recently observed leukemia in two patients with hemophilia A and B, 10 and 1½ years of age, respectively.2 Because commercial factor VIII and IX were not used and only blood, fresh frozen plasma, and plasma were given on a few occasions, it was less likely that AIDS-like immune changes were responsible for the leukemia in our patients.

scholarly journals 52 year Male with Haemophila-A; Presented with Scrotal Haematoma: Managed in Resource Constraint Settings - A Case Report

2019 ◽  
Vol 10 (2) ◽  
pp. 118-121
Author(s):  
Shoaeb Imtiaz Alam ◽  
Md Mustafizur Rahman ◽  
Ferdoush Rayhan ◽  
ASM Farhad Ul Hasan
Keyword(s):  
Factor Viii ◽  
Good Alternative ◽  
Fresh Frozen Plasma ◽  
Haemophilia A ◽  
Soft Tissue Trauma ◽  
Fresh Frozen ◽  
Secondary Closure ◽  
Tissue Trauma ◽  
The Cost ◽  
Frozen Plasma

Haemophilia A is an X linked disorder characterized by bleeding manifestations due to deficiency of factor VIII. Administration of factor VIII is the mainstay of treatment in case of bleeding which is very costly. That’s why fresh frozen plasma is a very good alternative in the management of mild to moderate bleeding. Here we present a case of 50 years old male presented to us with traumatic scrotal haematoma who was newly diagnosed with Haemophilia A. As the patient was unable to bear the cost of factor VIII, we managed the patient by transfusing fresh frozen plasma. After raising his activity of factor VIII up to 30% which was adequate for soft tissue trauma, surgical exploration of scrotum was done. Both the testes were found viable. Evacuation of clot was done followed by secondary closure of the wound J Shaheed Suhrawardy Med Coll, December 2018, Vol.10(2); 118-121

Effect of Whole Blood Storage on Factor VIII Recovery in Fresh Frozen Plasma and Cryoprecipitate

Vox Sanguinis ◽  
1996 ◽  
Vol 71 (3) ◽  
pp. 150-154 ◽  
Author(s):  
D. P. Allersma ◽  
R. M. R. Imambaks ◽  
L. J. Meerhof
Keyword(s):  
Factor Viii ◽  
Whole Blood ◽  
Fresh Frozen Plasma ◽  
Blood Storage ◽  
Fresh Frozen ◽  
Frozen Plasma

Use of Plasma Segments for Estimating Factor VIII Activity in Pools of Fresh Frozen Plasma

Vox Sanguinis ◽  
1987 ◽  
Vol 52 (3) ◽  
pp. 254-256
Author(s):  
F.A. Ofosu ◽  
L.M. Smith ◽  
M.A. Blajchman ◽  
J. (b) Campbell ◽  
C. De Vries ◽  
...  
Keyword(s):  
Factor Viii ◽  
Fresh Frozen Plasma ◽  
Factor Viii Activity ◽  
Fresh Frozen ◽  
Frozen Plasma

Is fresh frozen plasma overtransfused in the United States?

Transfusion ◽  
2004 ◽  
Vol 44 (11) ◽  
pp. 1674-1675 ◽  
Author(s):  
Jonathan P. Wallis ◽  
Sunny Dzik
Keyword(s):  
United States ◽  
The United States ◽  
Fresh Frozen Plasma ◽  
Fresh Frozen ◽  
Frozen Plasma

Hereditary Angioedema: Management of Laryngeal Attacks

2011 ◽  
Vol 25 (6) ◽  
pp. 379-382 ◽  
Author(s):  
Sandra C. Christiansen ◽  
Bruce L. Zuraw
Keyword(s):  
Hereditary Angioedema ◽  
Treatment Options ◽  
The United States ◽  
Fresh Frozen Plasma ◽  
C1 Inhibitor ◽  
Acute Therapy ◽  
Fresh Frozen ◽  
Life Threatening ◽  
Frozen Plasma ◽  
Future Management

Background Hereditary angioedema (HAE) patients suffering from laryngeal attacks in the United States faced severely limited treatment options until 2008. These potentially life-threatening episodes occur in over one-half of the patients affected by HAE during their lifetimes. Acute therapy had been relegated to supportive care, intubation, and consideration of fresh frozen plasma (FFP)–-the latter with the potential for actually accelerating the speed and severity of the swelling. Methods In this article we will review the recently approved and emerging HAE treatments that have evolved from the recognition that bradykinin generation is the fundamental abnormality leading to attacks of angioedema. Results Acute therapy for laryngeal attacks will be discussed including purified plasma–derived C1 inhibitor (C1INH), recombinant C1INH, an inhibitor of plasma kallikrein (ecallantide), and a B2 receptor antagonist (icatibant). Prophylactic care has also been transformed from a reliance on attenuated androgens with their attendant side effects to C1INH replacement. Conclusion The arrival of these novel therapies promises to transform the future management of HAE.

Use of Segments for the Quality Control of the Factor VIII: Coagulant Activity of Fresh Frozen Plasma

Vox Sanguinis ◽  
1985 ◽  
Vol 48 (4) ◽  
pp. 213-216
Author(s):  
F.A. Ofosu ◽  
M.A. Blajchman ◽  
A. Kaegi ◽  
J.M. Turc
Keyword(s):  
Quality Control ◽  
Factor Viii ◽  
Fresh Frozen Plasma ◽  
Coagulant Activity ◽  
Fresh Frozen ◽  
Frozen Plasma
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