Effects of Vancomycin on Platelets, Plasma Proteins and Hepatitis B Surface Antigen

1975 ◽  
Vol 34 (01) ◽  
pp. 083-093 ◽  
Author(s):  
Barry S Coller ◽  
W. B Lundberg ◽  
Harvey R Gralnick
Keyword(s):  
Hepatitis B ◽  
Factor Viii ◽  
Surface Antigen ◽  
Plasma Proteins ◽  
Hepatitis B Surface Antigen ◽  
Infusion Site ◽  
Vancomycin Therapy ◽  
Intravenous Injections ◽  
Low Concentrations ◽  
High Doses

SummaryThe antibiotic vancomycin shares many similarities with ristocetin, an agent noted for its effects on platelets and plasma fibrinogen. Vancomycin did not aggregate platelets as ristocetin, but platelets were incorporated into precipitates induced by vancomycin. Fibrinogen and factor VIII were precipitated from plasma at low concentrations of vancomycin. The precipitated fibrinogen remained clottable. Hepatitis B surface antigen was selectively precipitated from serum and could be recovered from the precipitate. Rabbits receiving bolus intravenous injections of high doses of vancomycin developed hypofibrinogenemia and thrombocytopenia within minutes and often went on to die. Studies with 125I-vancomycin revealed little stable binding of the antibiotic to platelets or fibrinogen. A relationship is suggested between the potent protein precipitating effects and phlebitis at the infusion site commonly associated with vancomycin therapy.

scholarly journals Relationship of large hepatitis B surface antigen polypeptide to human serum albumin

1980 ◽  
Vol 28 (2) ◽  
pp. 459-463
Author(s):  
J W Shih ◽  
P L Tan ◽  
J L Gerin
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Hepatitis B ◽  
Serum Albumin ◽  
Human Serum ◽  
Surface Antigen ◽  
Plasma Proteins ◽  
Hepatitis B Surface Antigen ◽  
Significant Proportion ◽  
Relationship Of

A significant proportion (20 to 40%) of highly purified 22-nm hepatitis B surface antigen (HBsAg) particles contain human serum albumin (HSA) as demonstrated by specific precipitation of radioiodinated particles by anti-HSA. Preparations of the isolated major HBsAg polypeptides (P-1, P-2, and P-6) were iodinated and analyzed by radiommunoprecipitation for reactivity with rabbit antisera to human plasma proteins. Only the P-6 fraction (molecular weight, 68,000) was precipitated and only by anti-HSA; specific precipitation was observed with guinea pig antisera to P-6 and native HBsAg and goat or rabbit antisera to HSA. Coprecipitation of P-6 with antiserum to HBsAg and with anti-HSA, compared to precipitation with each antiserum alone, indicated that the HBsAg and HSA determinants were on separate molecules. The P-6 polypeptide may represent a precursor protein of the hepatitis B virion.

Plasma Fibronectin Levels Identified via Quantitative Proteomics Profiling Predicts Hepatitis B Surface Antigen Seroclearance in Chronic Hepatitis B

2019 ◽  
Vol 220 (6) ◽  
pp. 940-950
Author(s):  
Fen Liu ◽  
Wai-Kay Seto ◽  
Danny Ka-Ho Wong ◽  
Fung-Yu Huang ◽  
Ka-Shing Cheung ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Surface Antigen ◽  
Plasma Proteins ◽  
Hepatitis B Surface Antigen ◽  
Protein Profiling ◽  
Plasma Fibronectin ◽  
Altered Expression ◽  
Hbsag Seroclearance

Abstract Background Seroclearance of hepatitis B surface antigen (HBsAg) is a potentially achievable target of chronic hepatitis B (CHB). Plasma proteins relevant to HBsAg seroclearance remain undetermined. Methods We prospectively recruited treatment-naive CHB patients with spontaneous HBsAg seroclearance and matched HBsAg-positive controls. Plasma protein profiling was performed using isobaric tags for relative and absolute quantitation-based proteomics, with the expression of candidate proteins validated in a separate cohort. The predictive value of fibronectin was assessed at 3 years, 1 year (Year -1) before, and at the time (Year 0) of HBsAg seroclearance. Results Four hundred eighty-seven plasma proteins were identified via proteomics, with 97 proteins showing altered expression. In the verification cohort (n = 90), median plasma fibronectin levels in patients with HBsAg seroclearance was higher than in controls (P = .009). In the longitudinal cohort (n = 164), patients with HBsAg seroclearance, compared with controls, had a higher median fibronectin levels at Year -1 (413.26 vs 227.95 µg/mL) and Year 0 (349.45 vs 208.72 µg/mL) (both P < .001). In patients with an annual HBsAg log reduction >0.5, Year -1 fibronectin level achieved an area under the receiving operator characteristic of 0.884 in predicting HBsAg seroclearance. Conclusions Using proteomics-based technology, plasma fibronectin may be associated with HBsAg seroclearance and a potential predictor of “functional cure”.

scholarly journals Hepatitis B Surface Antigen Testing in Plasmapheresis Donors

1977 ◽  
Author(s):  
Clyde B. McAuley
Keyword(s):  
Hepatitis B ◽  
Factor Viii ◽  
Surface Antigen ◽  
Second Generation ◽  
Hepatitis B Surface Antigen ◽  
Third Generation ◽  
Biological Products ◽  
Freeze Dried ◽  
Antigen Testing

It is well recognized that the safety of biological products prepared from blood, such as ProfilateR, a freeze-dried factor VIII concentrate, may be increased by testing for hepatitis B surface antigen.Abbott Laboratories has been routinely screening all plasma donations for hepatitis B surface antigen since January, 1971. Originally, the screening was performed using the second generation counter-immunoelectrophoresis technique, AUS-tect. In April, 1973, the counter-immunoelectrophoresis testing was replaced with the more sensitive third generation radioimmunoassay technique, Aus-RIA, for routine hepatitis B surface antigen testing. The most sensitive current radioimmunoassay technique, Aus-RIA II, has been used since February, 1975. This presentation will review the results of six years of testing experience. The data indicate that the incidence of positive donors in the commercial plasmapheresis system is 1.4 per 1,000, similar to that reported for a volunteer blood program.

scholarly journals Sgot Elevations and Hepatitis B Infections in Hemophiliacs

1977 ◽  
Author(s):  
E. Eyster ◽  
J. Haverstick ◽  
S. Plotkin
Keyword(s):  
Hepatitis B ◽  
Factor Viii ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen

SGOT, Hepatitis B surface antigen (Ag) and antibody (Ab) were performed at yearly or twice yearly intervals for 1-2 years on 75 hemophiliacs receiving an average of 40,000 Factor VIII units per patient per year. Of 42 receiving >10,000 VIII units on 5 or more days per year, 38 (90%) had SGOT elevations. In 30 (71%), abnormalities were recurrent. Of 33 receiving less Factor VIII, only 10 (30%) had recurrent SGOT elevations; all 10 had either Ab or persistent Ag.Fifteen patients received only cryoprecipitate. In 3/5 receiving >50 bags on 5 or more days per year, SGOT elevations persisted; one had persistent Ag and one had Ab. None of the 10 receiving less cryo had persistent SGOT elevations; 5 had Ab.Sixty-three (84%) were Ab pos. Recurrent SGOT elevations were seen in 35/63 (55%), including 7 with Ab titers >1000. Seven patients were Ab neg; none had an ↑ SGOT. All but one with agammaglobulinemia had received <50 transfusions per lifetime and <5000 VIII units on <3 days that year. Five of the 7 had received only cryo. Five patients (6%) were persistently Ag pos; all had persistent SGOT elevations.It was concluded that SGOT elevations in hemophiliacs were related to the frequency and intensity of Factor VIII infusions, and were regularly seen in patients receiving >10,000 VIII units or >50 bags of cryo on 5 or more days per year. Patients with Ab frequently had SGOT elevations while those with no Ab usually did not. Ab (or Ag) was found in 58/60 patients who received concentrate compared to 10/15 who received cryo only (p <.005).

IL10 Promotorpolymorphismen beeinflussen die Hepatitis B Surface Antigen und die Hepatitis A spezifische Immunantwort

2015 ◽  
Vol 41 (08) ◽  
Author(s):  
E Reuss ◽  
N Evers ◽  
N Dietrich ◽  
J Vollmar ◽  
PM Schneider ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis A ◽  
Hepatitis B Surface Antigen
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