The Effects of Prostacyclin on the Coagulation of Whole Blood

1983 ◽  
Vol 50 (03) ◽  
pp. 671-675 ◽  
Author(s):  
D S Holloway ◽  
L Zuckerman ◽  
J P Vagher ◽  
L F Mockros
Keyword(s):  
Mechanical Properties ◽  
Shear Modulus ◽  
Human Blood ◽  
Whole Blood ◽  
Clot Formation ◽  
Second Phase ◽  
Inhibitory Effects ◽  
Platelet Activity ◽  
Blood Samples ◽  
Human Blood Samples

SummaryThe effects of prostacyclin (PGI2) on mechanical properties of forming clots were investigated by testing human blood samples on a Thrombelastograph. Concentrations greater than 50 ng/ml (blood) caused a biphasic development of clot stiffness. During the first phase, PGI2 partially inhibited the platelet involvement in coagulation causing initial clot formation at a normal time but with reduced clot stiffness. The second phase occurred after neutralization of PGI2 activity and was characterized by recovery of platelet activity to produce a final clot with normal shear modulus. The duration of the inhibitory effects depended on PGI2 concentration and hematocrit. With a normal hematocrit, a PGI2 concentration of 60 ng/ml caused an inhibition for about 40 min whereas a concentration of 100 ng/ml caused inhibition for about 75 min.

A Modified Indirect Method for Determining Erythrocyte Sodium and Potassium Concentrations

1973 ◽  
Vol 19 (5) ◽  
pp. 506-510 ◽  
Author(s):  
Larry L Small ◽  
Dwight B Coulter
Keyword(s):  
Cell Volume ◽  
Human Blood ◽  
Whole Blood ◽  
Indirect Method ◽  
Indirect Methods ◽  
Blood Samples ◽  
Automated Method ◽  
Human Blood Samples ◽  
Erythrocyte Sodium ◽  
Sodium And Potassium

Abstract An automated method was adapted to measure the concentration of Na and K in plasma, nonhemolyzed whole blood, and hemolyzed whole blood, and thus allow the calculation of erythrocyte electrolyte concentrations by a modified indirect method. The Na concentrations of nonhemolyzed whole blood and plasma were used to calculate the percent cell volume (hematocrit) of a blood sample. The percent cell volume and concentrations of Na and K in nonhemolyzed whole blood were used to modify the indirect method of calculating erythrocyte Na and K concentrations in canine, porcine, and human blood samples. Significant differences were found between the two indirect methods (indirect and modified indirect) used to calculate erythrocyte Na and K concentrations of human blood samples.

scholarly journals Stabilization of gene expression in whole blood: high quality RNA with room temperature handling of human blood samples.

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Vasco Liberal ◽  
Angela Stassinopoulos ◽  
Scott Whitney ◽  
Steven Wilkinson ◽  
Winnie Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Blood ◽  
Whole Blood ◽  
Room Temperature ◽  
High Quality ◽  
Blood Samples ◽  
Human Blood Samples

scholarly journals Evaluation of a Technic for Counting Dog and Human Platelets

Blood ◽  
1965 ◽  
Vol 25 (2) ◽  
pp. 261-266 ◽  
Author(s):  
ROBERT I. WEED ◽  
S. LEE CRUMP ◽  
SCOTT N. SWISHER ◽  
Norma C. Trabold
Keyword(s):  
Human Blood ◽  
Whole Blood ◽  
Blood Platelets ◽  
Ammonium Oxalate ◽  
Human Platelets ◽  
Blood Samples ◽  
Platelet Counts ◽  
Statistical Errors ◽  
Human Blood Samples

Abstract 1. The addition of Na2EDTA (2.5 to 5 mg./2 ml. of whole blood) to dog or human blood samples has been shown to eliminate the problem of platelet clumping and to provide satisfactory samples for use in enumeration of blood platelets. The statistical errors of platelet counts in blood samples prepared with Na2EDTA agree well with previously published results obtained with unmodified technic. In blood samples prepared without Na2EDTA, where clumping is appreciable, the field error is inflated. 2. Variation in the time during which the platelets are permitted to settle in the chamber before counting, between limits of 30 and 90 minutes, has no effect on the count. 3. The period of standing of the diluted blood in 1 per cent ammonium oxalate, from 30 minutes to four hours, appears to have no effect on the out-come of the platelet counting results when the platelets are not clumped.

scholarly journals A frogspawn-like Ag@C core–shell structure for an ultrasensitive label-free electrochemical immunosensing of carcinoembryonic antigen in blood plasma

RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16339-16350
Author(s):  
Mengkui Ding ◽  
Ling Zha ◽  
Hui Wang ◽  
Jinyao Liu ◽  
Peiwu Chen ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Carcinoembryonic Antigen ◽  
Human Blood ◽  
Shell Structure ◽  
Core Shell ◽  
Label Free ◽  
Blood Samples ◽  
Human Blood Samples ◽  
Core Shell Structure ◽  
Electrochemical Immunosensing

Novel frogspawn-like Ag@C nanoparticles were successfully used to fabricate an ultrasensitive electrochemical immunosensing platform toward CEA in human blood samples.

Borrelia burgdorferi Survival in Human Blood Samples

1988 ◽  
Vol 539 (1 Lyme Disease) ◽  
pp. 444-445 ◽  
Author(s):  
GUY BARANTON ◽  
ISABELLE SAINT-GIRONS
Keyword(s):  
Borrelia Burgdorferi ◽  
Human Blood ◽  
Blood Samples ◽  
Human Blood Samples

scholarly journals Evaluation of pesticide residues in human blood samples from Punjab (India)

2015 ◽  
Vol 8 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Jasbir Singh Bedi ◽  
J. P. S. Gill ◽  
P. Kaur ◽  
A. Sharma ◽  
R. S. Aulakh
Keyword(s):  
Human Blood ◽  
Pesticide Residues ◽  
Blood Samples ◽  
Human Blood Samples
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