scholarly journals Further Experience on the Effect of High Pre-Donation Factor VIII Levels in Blood Donors on the Factor VIII Content of Small-Pool Fractions

1977 ◽  
Author(s):  
H. Beeser ◽  
H. Eqli
Keyword(s):  
Factor Viii ◽  
High Plasma ◽  
Activity Levels ◽  
Factor Viii Activity ◽  
Factor Viii Level ◽  
Plasma Factor ◽  
Viii Level ◽  
Factor Viii Concentrates ◽  
Small Pool ◽  
Concentrate Preparation

Because of the well known wide normal range of the factor VIII activity between 60 to 170% I man, selecting of donors with high activity levels would be of advantaae for the preparation of factor VIII concentrates. This is especially true for preparing small-pool fractions, as for technical reasons the final product cannot be controlled for its factor VIII content. In preliminary investigations, we reported on elsewhere, high factor VIII activity in donors estimated before a donation had been rarely reproducible before a second donation after 8-12 weeks. So as a preliminary result of finding a donor’s factor VIII level varying from donation to donation selecting of plasmas with high factor VIII content for concentrate preparation could only be establishedby re-estimating the activity before each donation. Proceeding in this way would be much too troublesome. To get more reliable information whether a healthy subject’s high factor VIII plasma level is distinctly varying or rather constant we assayed the plasma of 200 donors with factor VIII activity > 120% two times more before donation. The results confirmed our preliminary findings, especially the fact that a high plasma factor VIII activity in experienced donors was rarely reproducible when re-estimated before a second and third donation. As a consequence selecting of donors with high factor VIII procoaqulant activity for preparing small-pool factor VIII concentrates is impracticable.

An Improved Method of Making Factor VIII Concentrates

1979 ◽  
Author(s):  
G. Rock ◽  
D.S. Palmer ◽  
E.S. Tackaberry ◽  
M. Wickerhauser
Keyword(s):  
Factor Viii ◽  
Present Method ◽  
Batch Preparation ◽  
Improved Method ◽  
Factor Viii Activity ◽  
Peg 4000 ◽  
Plasma Factor ◽  
Factor Viii Concentrates ◽  
The Cost ◽  
Plasma Activity

The yields from batch preparation of Factor VIII concentrates can be substantially improved by collecting the blood into heparin rather than into CPD as anticoagulant. The resultant cryoprecipitate contains 78 ± 9% of the original plasma activity if 20 mls of supernatant per litre of starting plasma are left with the cryoprecipitate to maintain heparin levels. This cryoprecipitate was further purified by solubilization at 37°C for 5 minutes using 40 cc of saline per litre of starting plasma. This preparation was adjusted to pH 6.3 and 4.5% PEG 4000. Then, after removal of the precipitate by centrifugaron, the 4.5% PEG supernatant is adjusted to pH 6.0 and 11% PEG. The 11% PEG precipitate obtained after centrifugation is resolubilized in 1/100th the original plasma volume with buffer (0.1 M glycine, 20 mM citrate, 0.15 H saline) containing 1 unit of heparin per ml. Experiments using plasma pools containing 1-15 donor units gave yields ranging from 390-490 plasma Factor VIII equivalents per litre of the starting plasma. The final product retains an average of 90% of the initial Factor VIII activity after 24 hours at 22°C. It is believed that the present method could substantially reduce the cost of producing Factor VIII concentrates.

scholarly journals Extensive catastrophic thromboses from elevation of factor VIII

2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jacqueline Kropf ◽  
Sarah Cheyney ◽  
Josselin Vachon ◽  
Philip Flaherty ◽  
Mai Vo ◽  
...  
Keyword(s):  
Factor Viii ◽  
Cause Of Death ◽  
Rapid Onset ◽  
High Plasma ◽  
Clinical Syndrome ◽  
Renal Infarction ◽  
Factor Viii Level ◽  
Progressive Dyspnea ◽  
Viii Level ◽  
St Elevation

Catastrophic thrombotic syndrome, otherwise known as thrombotic storm (TS) is an extreme prothrombotic clinical syndrome that presents as rapid onset of multiple thromboembolic events affecting a large variety of vasculature. In recent studies, there has been a correlation of high plasma levels of factor VIII with thrombotic events. We present the case of a young man who exhibited multi-organ failure due to thrombotic storm. A 38-year-old male presented to the emergency department for progressive dyspnea and was diagnosed to have pulmonary embolism. The patient developed respiratory distress requiring intubation and was diagnosed with both an ST-elevation myocardial infarction and right cerebral infarction during the hospital course. The patient expired and autopsy revealed the cause of death to be myocardial, cerebral and renal infarction from widespread vascular thrombosis. Autopsy revealed cause of death to be elevated factor VIII associated thrombotic coagulopathy. Factor VIII level upon autopsy was 375% (55-200%). Although TS is rare, it can be lifethreatening if not recognized early. Survival depends on the prompt initiation and duration of anticoagulation.

Laboratory Detection of Female Carriers of Canine Hemophilia

1964 ◽  
Vol 12 (02) ◽  
pp. 368-376 ◽  
Author(s):  
B. J Parks ◽  
K. M Brinkhous ◽  
P. F Harris ◽  
G. D Penick
Keyword(s):  
Factor Viii ◽  
Partial Thromboplastin Time ◽  
Screening Test ◽  
Factor Viii Level ◽  
Viii Level ◽  
Antihemophilic Factor ◽  
Female Carriers

SummaryFemales known to be heterozygous for canine hemophilia had a plasma antihemophilic factor (AHF, factor VIII) level of about 50%, as determined by bioassay and by the effectiveness of their transfused plasma in raising the AHF levels of hemophilic dogs. Determination of the plasma AHF should serve to identify transmitter females prior to appearance of affected progeny in litters. Lyon’s hypothesis appears to apply to our findings.The simple partial thromboplastin time (PTT) test was prolonged in heterozygous females. Modifications of the test, by the addition of thrombin, a serum accelerator preparation, or kaolin, gave consistently longer PTT values for heterozygotes than for normal dogs. The PTT appears useful as a screening test for carriers of canine hemophilia.

Factor-VIII Inhibitor in Less Severely Affected Haemophilic Patients

1975 ◽  
Author(s):  
E. G. D. Tuddenham ◽  
A. L. Bloom ◽  
J. C. Giddings ◽  
C. A. Barrett
Keyword(s):  
Factor Viii ◽  
Factor Viii Inhibitor ◽  
Factor Viii Level ◽  
Replacement Treatment ◽  
Viii Level ◽  
Viii Inhibitor ◽  
In Vivo Recovery ◽  
Better Than

The occurrence of factor VIII inhibitor in five mild or moderately affected liaemophilic patients is described. In four patients the inhibitor inactivated endogenous factor VIII an dtemporarily converted them to severely affected haemophiliacs with factor VIII level of 0%. In the fifth patient, a brother of one of the others, the inhibitor although more potent did not inactivate the patient’s own factor VIII and did not completely inactivate normal factor VIII in vitro. This patient responded to treatment with factor-VIII concentrate but the in-vivo recovery was reduced. The patient’s plasma was tested against a panel of normal donors but it inactivated factor VIII in each to a similar extent and no evidence for normal factor-VIII groups was obtained. In the other patients the response to replacement treatment was also better than that usually seen in severely affected haemophilic patients with inhibitor. In the two related patients the inhibitors have so far persisted but in the unrelated patients the inhibitors eventually disappeared and did not always recur with subsequent therapy. The incidence of factor- VIII inhibitor in less severe haemophiliacs (factor VIII > 3% ) in this centre is 6% suggesting that the complication is more frequent in this type of patient than hitherto recognised.

Hematogenous thrombophilia as an additional risk factor for cerebral circulation disorders

2021 ◽  
Author(s):  
И.В. Куртов ◽  
С.П. Кривова ◽  
Р.К. Хайретдинов ◽  
И.Л. Давыдкин
Keyword(s):  
Risk Factor ◽  
Factor Viii ◽  
Blood Coagulation ◽  
Genetic Polymorphisms ◽  
Additional Risk Factor ◽  
Factor Viii Level ◽  
Aged Patients ◽  
Additional Risk ◽  
Viii Level ◽  
The Impact

Введение. Гематогенная тромбофилия служит дополнительным фактором риска нарушений мозгового кровообращения (НМК). Цель исследования: изучить влияние повышенного уровня фактора VIII и нарушений в системе фибринолиза на НМК у пациентов с сочетанной патологией системы свертывания крови. Материалы и методы. Обследовано 20 пациентов с перенесенными ишемическими инсультами в возрасте от 36 до 56 лет. Определяли показатели плазменного, тромбоцитарного звеньев гемостаза, системы фибринолиза, а также генетические полиморфизмы системы гемостаза. Результаты. У всех пациентов выявлены различные сочетания генетических полиморфизмов, связанных с изменениями антикоагулянтного звена гемостаза, а также дефекты системы фибринолиза и коагуляционного звена гемостаза, что является дополнительным фактором риска НМК. Заключение. У пациентов молодого и среднего возраста, перенесших острое НМК по ишемическому типу, необходимо исследование системы свертывания крови, включающее определение содержания фактора VIII в крови, показателей системы фибринолиза и уровня гомоцистеина. Background. Hematogenous thrombophilia is an additional risk factor for cerebral circulatory disorders (ССD). Objectives: to study the impact of increased factor VIII level and disturbances of fibrinolysis system on ССD in patients with combined pathology of blood coagulation. Patients/Methods. We examined 20 patients with ischemic strokes aged from 36 to 56 years. The parameters of plasma hemostasis, platelet function, fibrinolysis system, as well as the genetic polymorphisms of hemostasis system were determined. Results. All patients had different combinations of genetic polymorphisms associated with anticoagulant hemostasis changes, as well as defects in fibrinolysis system and coagulation hemostasis that is an additional risk factor for ischemic stroke. Conclusions. In young and middle-aged patients with history of acute ischemic ССD, it is necessary to study blood coagulation, including the determination of blood factor VIII level, fibrinolysis system parameters and homocysteine content.

Fibrinolytic and F. VIII Response to Various Stimuli in Occlusive Arterial Diasease

1975 ◽  
Author(s):  
O. Ponari ◽  
E. Civardi ◽  
R. Potì ◽  
A. G. Dettori
Keyword(s):  
Normal Subjects ◽  
Arterial Disease ◽  
Venous Occlusion ◽  
Control Group ◽  
Factor Viii Level ◽  
Average Decrease ◽  
Average Value ◽  
Occlusive Arterial Disease ◽  
Plasma Factor ◽  
Viii Level

The responsiveness of fibrinolytic activity and of plasma Factor VIII level to both venous occlusion (v.o.) and i.v. nicotinic acid (N. A.) was investigated in a group of patients with occlusive arterial disease.A marked hyperfibrinolysis, with average decrease of ELT of -55%, was seen after v.o. in such patients, with a rise of F. VIII (average value +30%). A control group of comparable age but with no signs of atherosclerosis, showed similar changes after v.o. (ELT about —65% and F. VIII +60%). A comparable hyperfibrinolytic response was also found in a group of young ( < 40 y.) normal subjects.I.V. administration of N. A. (100 mg) obtained variations of ELT of similar magnitude (—50%) in both groups (patients and age-matched controls). Changes in F. VIII were absent or only moderate.The responsiveness to v.o. in single cases showed no correlation with an index derived from main risk factors (both clinical and biochemical) for thrombosis.Our results do not agree with the hypotesis that an altered responsiveness of vascular fibrinolytic system is an important factor in the pathogenesis of occlusive arterial disease.

scholarly journals Clinical Severity, Personality Characteristics and Maternal Attitudes in Hemophiliac Children

1977 ◽  
Author(s):  
L.L. Ackerman ◽  
J. Verrecchio ◽  
D.S. Charney ◽  
E.M. Strickler
Keyword(s):  
Factor Viii ◽  
Personality Characteristics ◽  
Clinical Severity ◽  
Personality Questionnaire ◽  
Factor Viii Level ◽  
Future Studies ◽  
Maternal Attitudes ◽  
Child Relationship ◽  
Viii Level ◽  
Mother Child Relationship

The interrelationships of severity of hemophilia, personality characteristics, and maternal attitudes were evaluated in 21 hemophiliac boys and 17 of their mothers. The 8 to 11 year old boys were given the Children’s Personality Questionnaire and those between 12 and 18, the Junior-Senior High School Personality Questionnaire. The mothers were administered the Mother-Child Relationship Evaluation which measured maternal attitudes of four scales; Acceptance (A), Overprotection (OP), Overindulgence (OI), and Rejection (R).Results were analyzed by comparing children with Factor VIII levels >1% (N=10) to those whose levels were <1% (N=ll) as determined on more than one occasion. There were no significant differences between the two groups in terms of personality characteristics or maternal attitudes.Results were also analyzed by comparing those children (N=6) who had accepting mothers (A> 60, OI < 30, OP < 30, R < 30) with those of non-accepting mothers (N=ll). The children of the non-accepting mothers (mean Factor VIII levels 3.0%) were significantly more sober (P<.02), and internally restrained (P <.01) and tended to be less intelligent and less emotionally stable than the children of accepting mothers (mean Factor VIII level 1.5%).These findings suggest that personality characteristics may be more closely related to maternal attitudes than severity of disease. Future studies should identify and focus on determinants of maternal attitudes.

scholarly journals Postpartum Acquired Hemophilia: A Rare Cause of Postpartum Hemorrhage

2013 ◽  
Vol 2013 ◽  
pp. 1-2 ◽  
Author(s):  
Srikanth Seethala ◽  
Sumit Gaur ◽  
Elizabeth Enderton ◽  
Javier Corral
Keyword(s):  
Factor Viii ◽  
Hemophilia A ◽  
Factor Vii ◽  
Normal Vaginal Delivery ◽  
Factor Viii Inhibitor ◽  
Activity Levels ◽  
Factor Viii Activity ◽  
Acquired Hemophilia A ◽  
Acquired Hemophilia ◽  
Viii Inhibitor

A 36-year-old female started having postpartum vaginal bleeding after normal vaginal delivery. She underwent hysterectomy for persistent bleeding and was referred to our institution. An elevation of PTT and normal PT made us suspect postpartum acquired hemophilia (PAH), and it was confirmed by low factor VIII activity levels and an elevated factor VIII inhibitor. Hemostasis was achieved with recombinant factor VII concentrates and desmopressin, and factor eradication was achieved with cytoxan, methylprednisolone, and plasmapheresis.

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