In vitroBehaviour of Sesquiterpene Lactones and Sesquiterpene Lactone-Containing Plant Preparations in Human Blood, Plasma and Human Serum Albumin Solutions

Neuroprotection from oxidative stress is critical during neuronal development and maintenance but also plays a major role in the pathogenesis and potential treatment of various neurological disorders and neurodegenerative diseases. Emerging evidence in the murine system suggests neuroprotective effects of blood plasma on the aged or diseased brain. However, little is known about plasma-mediated effects on human neurons. In the present study, we demonstrate the neuroprotective effect mediated by human plasma and the most abundant plasma–protein human serum albumin against oxidative stress in glutamatergic neurons differentiated from human neural crest-derived inferior turbinate stem cells. We observed a strong neuroprotective effect of human plasma and human serum albumin against oxidative stress-induced neuronal death on the single cell level, similar to the one mediated by tumor necrosis factor alpha. Moreover, we detected neuroprotection of plasma and human serum albumin against kainic acid-induced excitatory stress in ex vivo cultured mouse hippocampal tissue slices. The present study provides deeper insights into plasma-mediated neuroprotection ultimately resulting in the development of novel therapies for a variety of neurological and, in particular, neurodegenerative diseases.

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Glutathione-Linked Thiol Peroxidase Activity of Human Serum Albumin: A Possible Antioxidant Role of Serum Albumin in Blood Plasma

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1996.1234 ◽

1996 ◽

Vol 225 (2) ◽

pp. 695

Author(s):

Mee-Kyung Cha ◽

Il-Han Kim

Keyword(s):

Human Serum Albumin ◽

Serum Albumin ◽

Blood Plasma ◽

Human Serum ◽

Peroxidase Activity ◽

Thiol Peroxidase ◽

In Blood Plasma

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Transfer of Radioiodinated Human Serum Albumin (RISA(R)) from Cerebrospinal Fluid to Blood Plasma.

Experimental Biology and Medicine ◽

10.3181/00379727-103-25643 ◽

1960 ◽

Vol 103 (4) ◽

pp. 708-710 ◽

Cited By ~ 18

Author(s):

C. A. Van Wart ◽

J. R. Dupont ◽

L. Kraintz

Keyword(s):

Cerebrospinal Fluid ◽

Human Serum Albumin ◽

Serum Albumin ◽

Blood Plasma ◽

Human Serum

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Investigation of relaxation times in 5-fluorouracil and human serum albumin mixtures

Turkish Journal of Biochemistry ◽

10.1515/tjb-2017-0363 ◽

2019 ◽

Vol 44 (4) ◽

pp. 524-529 ◽

Cited By ~ 1

Author(s):

Sibel Korunur ◽

Bilgin Zengin ◽

Ali Yilmaz

Keyword(s):

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Relaxation Times ◽

Solution Temperature ◽

Cancer Drug ◽

Human Blood Plasma ◽

Albumin Concentration ◽

Protein Drug ◽

Relaxation Rates

Abstract Background Human serum albumin (HSA) is often selected as a subject of any study because albumin is the most abundant protein in human blood plasma. NMR is recognized as a valuable method to determine the structure of proteins-ligand and protein-drug complexes. Objective – Aim of the study In this study, protein drug interactions were investigated using 5-Fluorouracil anti-cancer drug and human serum albumin protein. Materials and methods In this context 400 MHz NMR spectrometry was used and NMR relaxation rates in drug-albumin complex were investigated with respect to increase albumin concentration and increase in 5-Fluorouracil (5-FU)-albumin solution temperature. Results The results of this study indicated that 5-FU had a weak association with albumin, and it easily dissociated from the protein to which it was attached. Conclusion The obtained results also gave us useful information about molecular dynamics of drug-albumin interactions.

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