Scanning Electron Microscopy of Microarterial Anastomoses with a Diode Laser: Comparison With Conventional Manual Suture

This study aimed to determine the effects of various lasers on dental implants’ surface characteristics. Nine explanted dental implants were included. Two implants were randomly allocated to four intervention groups, namely, diode (2 W, 810 nm, 10 s), CO2 (2 W, 10600 nm, 10 s), Er : YAG (200 mJ/20 Hz, 2940 nm, 10 s), and Er, Cr : YSGG (200 mJ/20 Hz, 2780 nm, 10 s) groups and one control group. After laser irradiation, all implants were imaged with scanning electron microscopy. Qualitative changes on the surface of implants were evaluated. Quantitative surface changes at the threads and between the threads were assessed by software using depression and prominence plots. The paired t-test was used for statistical analysis. Diode laser irradiation showed the least surface changes while the Er : YAG group showed the greatest surface changes. Furthermore, CO2 and Er : YAG laser irradiation significantly altered the mean profile area at the threads ( p < 0.05 ), while CO2 and Er, Cr : YSGG laser irradiation significantly altered the mean profile area between the threads ( p < 0.05 ). Diode laser irradiation does not alter the implant surface characteristics. However, the use of CO2, Er : YAG, and Er, Cr : YSGG lasers on titanium implant surfaces is discouraged as they damage the titanium implant surfaces.

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Apical microleakage and SEM analysis of dentin surface after 980 nm diode laser irradiation

Brazilian Dental Journal ◽

10.1590/s0103-64402011000500006 ◽

2011 ◽

Vol 22 (5) ◽

pp. 382-387 ◽

Cited By ~ 11

Author(s):

Maria Isabel Anastácio Faria ◽

Aline Evangelista Souza-Gabriel ◽

Edson Alfredo ◽

Danielle Cristine Furtado Messias ◽

Yara Teresinha Correa Silva-Sousa

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Laser Irradiation ◽

Diode Laser ◽

Laser Power ◽

Sem Analysis ◽

Gutta Percha ◽

Ah Plus ◽

Apical Leakage ◽

Scanning Electron

This study evaluated the effect of 980-nm diode laser on apical microleakage and intraradicular dentin morphology. Roots of 110 mandibular incisors were used in the study: 92 for microleakage test and 18 for scanning electron microscopy (SEM). Roots were randomly assigned to 3 groups according to the irrigating solution (water, NaOCl and NaOCl/EDTA) and were divided into 3 subgroups according to the laser irradiation protocol (without irradiation, irradiated at 1.5 W and irradiated at 3.0 W). Two specimens of each subgroup were prepared for SEM. The remaining roots were filled with AH Plus and gutta-percha. Apical leakage was assessed by ink penetration and data were analyzed statistically by ANOVA and Tukey-Krammer test (α=0.05). SEM analysis showed intensification of changes with increase of laser power as well as variations according to the irrigating solution. Modified smear layer was observed in specimens treated with water and irradiated with laser. Roots irrigated with NaOCl/EDTA had lower levels of infiltration (0.17 ± 0.18 mm) differing significantly (p<0.05) from those of roots irrigated with water (0.34 ± 0.30 mm), but similar (p>0.05) to those irrigated with NaOCl (0.28 ± 0.29 mm). Non-irradiated roots had lower levels of infiltration (0.10 ± 0.14 mm), differing (p<0.05) from those irradiated at 1.5 W (0.32 ± 0.22 mm) and 3.0 W (0.37 ± 0.32 mm). The 980 nm diode laser modified dentin morphology and increased apical microleakage.

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Pathogenesis of Human Hair Defects

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005007x ◽

1974 ◽

Vol 32 ◽

pp. 12-13

Author(s):

P.S. Porter ◽

T. Aoyagi ◽

R. Matta

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Human Hair ◽

Standard Techniques ◽

Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

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Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080614 ◽

1977 ◽

Vol 35 ◽

pp. 638-639

Author(s):

P.J. Dailey

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Salivary Glands ◽

Secretory Vesicles ◽

The Past ◽

Transmission Electron ◽

Means Of Transmission ◽

Gromphadorhina Portentosa ◽

Scanning Electron ◽

Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081279 ◽

1978 ◽

Vol 36 (2) ◽

pp. 82-83 ◽

Cited By ~ 1

Author(s):

Nakazo Watari ◽

Yasuaki Hotta ◽

Yoshio Mabuchi

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Transmission Electron Microscopy ◽

Fine Structure ◽

Light Microscopy ◽

Thin Sections ◽

Transmission Electron ◽

Identical Cell ◽

Electron Microscopes ◽

Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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Spontaneous Cataract in Nude Athymic Mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079991 ◽

1977 ◽

Vol 35 ◽

pp. 512-513

Author(s):

Ronald H. Bradley ◽

R. S. Berk ◽

L. D. Hazlett

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Nude Mouse ◽

Cellular Immunity ◽

Phosphate Buffer ◽

Osmium Tetroxide ◽

Athymic Mice ◽

Transmission Microscopy ◽

Mouse Lens ◽

Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100066097 ◽

1971 ◽

Vol 29 ◽

pp. 410-411 ◽

Cited By ~ 1

Author(s):

Jane A. Westfall ◽

S. Yamataka ◽

Paul D. Enos

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Osmium Tetroxide ◽

External Surface ◽

Three Dimensional ◽

Surface Structures ◽

Transmission Microscopy ◽

Transmission Electron ◽

Freeze Dried ◽

Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

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