Gene expression of enzymes related to biosynthesis of antioxidative flavonoids in Siamese neem tree leaves

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
P Sithisarn ◽  
M Suksangpanomrung ◽  
W Gritsanapan
Keyword(s):  
Gene Expression ◽  
Tree Leaves ◽  
Neem Tree

scholarly journals Repellency Effect of Prepared Neem Tree Leaves Smoke against Mosquito

2020 ◽  
pp. 1-6
Author(s):  
M. M. Jibrin ◽  
A. D. Mohammed
Keyword(s):  
Adverse Effect ◽  
Rating Scale ◽  
Distilled Water ◽  
Tree Leaves ◽  
Neem Tree ◽  
Constant Weight ◽  
Prepared Sample ◽  
Time Of Application ◽  
Neem Leaves ◽  
Before And After

The study was design to evaluate the smoke repellency effect of prepared neem leaves, against mosquito. The dried neem powder was used for making the insecticide, 10 g of powdered leaves sample was weight into a 200 ml beaker, and 5.0 g of starch was weight and added to the leave sample in the beaker. The mixture was stirred and 5.0 ml of distilled water was added gradually to the mixture in little quantity while stirring. The beaker with wet insecticide mixture was dried in an oven until a constant weight was obtained. The prepared neem insecticide was distributed among the households to use at night, to substitute synthetic insecticides. The users were randomly selected within the study area. The data was collected based on the time of application, number of bites receive before and after the application this was done based on 0-5 rating scale. The result revealed that the prepared insecticide is 100% effective though the effectiveness is dependent to time taken after application. The questionnaire was also used to assess the view of the users on the adverse effect they experienced upon the exposure to neem tree leaves prepared sample. The study has been concluded that neem leaves smoke is effective in repelling mosquito, and it can be an alternative to the synthetic insecticides hence it is safe for the users and the environment.

Molecular and Microscopic Analysis of Altered Gene Expression in Transgenic and Gene Targeted Mice

1996 ◽  
Vol 54 ◽  
pp. 12-13
Author(s):  
W. K. Jones ◽  
J. Robbins
Keyword(s):  
Gene Expression ◽  
Pulmonary Veins ◽  
Microscopic Analysis ◽  
Mouse Tissue ◽  
Adult Heart ◽  
Heavy Chains ◽  
Altered Gene Expression ◽  
Altered Gene ◽  
Pulmonary Myocardium

Two myosin heavy chains (MyHC) are expressed in the mammalian heart and are differentially regulated during development. In the mouse, the α-MyHC is expressed constitutively in the atrium. At birth, the β-MyHC is downregulated and replaced by the α-MyHC, which is the sole cardiac MyHC isoform in the adult heart. We have employed transgenic and gene-targeting methodologies to study the regulation of cardiac MyHC gene expression and the functional and developmental consequences of altered α-MyHC expression in the mouse.We previously characterized an α-MyHC promoter capable of driving tissue-specific and developmentally correct expression of a CAT (chloramphenicol acetyltransferase) marker in the mouse. Tissue surveys detected a small amount of CAT activity in the lung (Fig. 1a). The results of in situ hybridization analyses indicated that the pattern of CAT transcript in the adult heart (Fig. 1b, top panel) is the same as that of α-MyHC (Fig. 1b, lower panel). The α-MyHC gene is expressed in a layer of cardiac muscle (pulmonary myocardium) associated with the pulmonary veins (Fig. 1c). These studies extend our understanding of α-MyHC expression and delimit a third cardiac compartment.

Linking transcription, RNA polymerase II elongation and alternative splicing

2020 ◽  
Vol 477 (16) ◽  
pp. 3091-3104 ◽  
Author(s):  
Luciana E. Giono ◽  
Alberto R. Kornblihtt
Keyword(s):  
Gene Expression ◽  
Alternative Splicing ◽  
Rna Polymerase Ii ◽  
Environmental Changes ◽  
Transcription Elongation ◽  
Single Gene ◽  
Regulation Of Gene Expression ◽  
Regulation Of Transcription ◽  
Stepping Stones ◽  
Eukaryotic Transcription

Gene expression is an intricately regulated process that is at the basis of cell differentiation, the maintenance of cell identity and the cellular responses to environmental changes. Alternative splicing, the process by which multiple functionally distinct transcripts are generated from a single gene, is one of the main mechanisms that contribute to expand the coding capacity of genomes and help explain the level of complexity achieved by higher organisms. Eukaryotic transcription is subject to multiple layers of regulation both intrinsic — such as promoter structure — and dynamic, allowing the cell to respond to internal and external signals. Similarly, alternative splicing choices are affected by all of these aspects, mainly through the regulation of transcription elongation, making it a regulatory knob on a par with the regulation of gene expression levels. This review aims to recapitulate some of the history and stepping-stones that led to the paradigms held today about transcription and splicing regulation, with major focus on transcription elongation and its effect on alternative splicing.

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