Yield limiting potential of necrotic and non-necrotic strains of Bean yellow mosaic virus in narrow-leafed lupin (Lupinus angustifolius)

2003 ◽  
Vol 54 (9) ◽  
pp. 849 ◽  
Author(s):  
R. A. C. Jones ◽  
B. A. Coutts ◽  
Y. Cheng
Keyword(s):  
Mosaic Virus ◽  
Seed Yield ◽  
Field Experiments ◽  
Seed Transmission ◽  
Lupinus Angustifolius ◽  
Bean Yellow Mosaic Virus ◽  
Early Infection ◽  
Lupinus Luteus ◽  
Yellow Mosaic Virus ◽  
Seed Number

The yield losses caused by necrotic and non-necrotic strains of Bean yellow mosaic virus (BYMV) in narrow-leafed lupin (Lupinus angustifolius) were quantified in field experiments. Clover plants infected with either were introduced into plots to provide infection sources, and aphids spread infection to the lupin plants. When the effects of virus infection were examined in individual lupin plants infected with necrotic BYMV, they were killed by early infection so there was no seed production. With late infection, shoot dry wt, seed yield, and seed number were decreased by at least 55%, 80%, and 74%, respectively. With non-necrotic BYMV, shoot dry wt, seed yield, and seed number diminished with increasing duration of plant infection, these decreases ranging over 27–88%, 48–99%, and 35–98% for late to early infection, respectively. In partially infected stands in which both necrotic and non-necrotic BYMV were spreading, an additional incidence of 28% in plots with introduced non-necrotic strain foci over that in plots without introduced foci was sufficient to decrease overall seed yield significantly. However, an additional incidence of 10% was insufficient to do so in plots with introduced necrotic strain foci. In plots into which different numbers of clover plants infected with non-necrotic BYMV were introduced, subsequent incidence of infection depended on the magnitude of the initial virus source, and yield was decreased by 21–24%, 31–43%, and 64–66% with 4, 8, or 16 foci/plot, respectively. With both types of strain, yield loss in infected plants was mainly due to failure to produce any seed or to fewer seeds being produced, but smaller seed size also contributed. These results show that non-necrotic strains of BYMV have considerable yield-limiting potential in narrow-leafed lupin crops despite causing milder symptoms than necrotic strains. No evidence was obtained of seed-transmission of non-necrotic BYMV in narrow-leafed lupin, but a 0.2% seed transmission rate was detected in yellow lupin (Lupinus luteus).

scholarly journals First Report of Bean yellow mosaic virus from Diseased Lupinus luteus in Eastern Washington

Plant Disease ◽  
2009 ◽  
Vol 93 (3) ◽  
pp. 319-319 ◽  
Author(s):  
N. L. Robertson ◽  
C. J. Coyne
Keyword(s):  
Mosaic Virus ◽  
Agricultural Research ◽  
Bean Yellow Mosaic Virus ◽  
Lupinus Luteus ◽  
Yellow Mosaic Virus ◽  
Universal Primers ◽  
Chenopodium Amaranticolor ◽  
Western Blots ◽  
First Report ◽  
Seed Collection

Lupine accessions from the Cool Season Food Legume Seed Collection are grown for seed regenerations in Pullman, WA by the Agricultural Research Service, Western Regional Plant Introduction Station. Selected seed was germinated in the greenhouse and assayed by indirect ELISA using antiserum for potyvirus group detection (Agdia, Inc., Elkhart, IN). Healthy transplants were grown for seed collection on outside plots. In July of 2005, more than 90% of 307 Lupinus luteus L. transplants developed severe yellowing, necrosis, and stunting with an estimated 5% plant death. Plants were heavily infested with aphids and leaf sap was serologically positive for potyvirus. Partially purified virus preparations from infected plants contained filamentous particles and a 35-kDa protein that reacted with universal potyvirus antiserum on western blots. Reverse transcription (RT)-PCR using potyvirus universal primers (2) and cDNA derived from virion RNA generated a ~1.7-kbp product that was cloned and sequenced. The sequenced portion of the genomic RNA contained 1,610 nucleotides (nt) on its 3′-terminus (GenBank Accession No. EU144223) that included a partial nuclear inclusion protein, NIb, (1 to 637 nt) with the conserved amino acid (aa) replicase motif GDD (131 to 139 nt), the coat protein (CP) gene of 821 nt (638 to 1,459 nt), and a 171-nt untranslated region (1,460 to 1,630 nt) attached to a poly(A)tail. The CP sequence contained a NAG motif instead of the DAG motif commonly associated with aphid transmission. Searches in the NCBI GenBank database revealed that the CP aa and nt sequences contained conserved domains with isolates of Bean yellow mosaic virus (BYMV). A pairwise alignment (ClustalX) (4) of the CP aa from 20 BYMV isolates with the BYMV-Pullman isolate revealed identities from 96% (BYMV-S, U47033) to 88% (BYMV-MI [X81124)] -MI-NAT [AF434661]). This meets the species demarcation criteria of more than ~80% identity for inclusion with BYMV (1). Virion mechanical inoculations resulted in local lesions on Chenopodium amaranticolor Coste et Reyn and C. quinoa Willd., necrotic blotches on Phaseolus vulgaris L., and yellow spots and systemic movement in L. succulentus Douglas ex. K. Koch, L. texensis ‘Bluebonnet’, and L. texensis ‘Maroon’; BYMV was confirmed by western blots and ELISA. The experimental inoculations represent the first documented report of BYMV in the annual L. succulentus and biennial L. texensis species. Since BYMV is seedborne and transmitted by many aphid species (3), it is possible that several lupine transplants escaped potyvirus detection, and secondary transmission of BYMV to plants occurred by aphids. During the 1950s, BYMV was confirmed in several annual lupines grown as crops in the southeastern United States (3). To our knowledge, this is the first report of BYMV occurring naturally in a lupine species in Washington. BYMV is a destructive virus to lupine species worldwide and has a wide host range in Fabaceae. This research directly contributes toward the maintenance of virus-free lupine seed for distribution to scientists focusing on lupine research. References: (1) P. H. Berger et al. Family Potyviridae. Page 819 in: Virus Taxonomy: Eighth Report of the ICTV. C. M. Fauquet et al. eds., 2005. (2) J. Chen et al. Arch. Virol. 146:757, 2001. (3) R. A. C. Jones and G. D. Mclean, Ann. Appl. Biol. 114:609, 1989. (4) J. D. Thompson et al. Nucleic Acids Res. 24:4878, 1997.

scholarly journals Seed Transmission of Bean Yellow Mosaic Virus in Broad Bean (Vicia faba).

1993 ◽  
Vol 59 (5) ◽  
pp. 559-562 ◽  
Author(s):  
Takahide SASAYA ◽  
Mabito IWASAKI ◽  
Takashi YAMAMOTO
Keyword(s):  
Mosaic Virus ◽  
Vicia Faba ◽  
Broad Bean ◽  
Seed Transmission ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

scholarly journals Black Pod Syndrome of Lupinus angustifolius Is Caused by Late Infection with Bean yellow mosaic virus

Plant Disease ◽  
2014 ◽  
Vol 98 (6) ◽  
pp. 739-745 ◽  
Author(s):  
M. A. Kehoe ◽  
B. J. Buirchell ◽  
B. A. Coutts ◽  
R. A. C. Jones
Keyword(s):  
Mosaic Virus ◽  
Lupinus Angustifolius ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Growth Stages ◽  
Late Infection ◽  
Rt Pcr ◽  
Tissue Samples ◽  
Black Pod

Black pod syndrome (BPS) causes devastating losses in Lupinus angustifolius (narrow-leafed lupin) crops in Australia, and infection with Bean yellow mosaic virus (BYMV) was suggested as a possible cause. In 2011, an end-of-growing-season survey in which L. angustifolius plants with BPS were collected from six locations in southwestern Australia was done. Tissue samples from different positions on each of these symptomatic plants were tested for BYMV and generic potyvirus by enzyme-linked immunosorbent assay and reverse-transcription polymerase chain reaction (RT-PCR). Detection was most reliable when RT-PCR with generic potyvirus primers was used on tissue taken from the main stem of the plant just below the black pods. Partial coat protein nucleotide sequences from eight isolates from BPS-symptomatic L. angustifolius plants all belonged to the BYMV general phylogenetic group. An initial glasshouse experiment revealed that mechanical inoculation of L. angustifolius plants with BYMV after pods had formed caused pods to turn black. This did not occur when the plants were inoculated before this growth stage (at first flowering) because BYMV infection caused plant death. A subsequent experiment in which plants were inoculated at eight different growth stages confirmed that BPS was only induced when L. angustifolius plants were inoculated after first flowering, when pods had formed. Thus, BYMV was isolated from symptomatic L. angustifolius survey samples, inoculated to and maintained in culture hosts, inoculated to healthy L. angustifolius test plants inducing BPS, and then successfully reisolated from them. As such, Koch's postulates were fulfilled for the hypothesis that late infection with BYMV causes BPS in L. angustifolius plants.

scholarly journals The Complete Nucleotide Sequence of Bean Yellow Mosaic Virus Genomic RNA.

1996 ◽  
Vol 62 (5) ◽  
pp. 472-477 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Ryoso HONKURA ◽  
Takayoshi IWAI ◽  
Masashi UGAKI ◽  
Yuko OHASHI
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Genomic Rna

scholarly journals PCNA Interacts with Indian Mung Bean Yellow Mosaic Virus Rep and Downregulates Rep Activity

2004 ◽  
Vol 78 (21) ◽  
pp. 11890-11903 ◽  
Author(s):  
Basavaraj Bagewadi ◽  
Shoajiang Chen ◽  
Sunil K. Lal ◽  
Nirupam Roy Choudhury ◽  
Sunil K. Mukherjee
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nuclear Antigen ◽  
Resting Cells ◽  
Rolling Circle Replication ◽  
Proliferative Cell Nuclear Antigen ◽  
Bipartite Genome

ABSTRACT Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detail. The initiation of RCR of the bipartite genome of a geminivirus, Indian mung bean yellow mosaic virus (IMYMV), is mainly controlled by viral protein Rep (or AL1 or AC1). The role of host PCNA in RCR of IMYMV was revealed by studying the physical and functional interactions between recombinant PCNA and recombinant IMYMV Rep. Pea nuclear PCNA as well as recombinant pea PCNA showed binding to recombinant Rep in experiments involving both affinity chromatography and yeast two-hybrid approaches. The contacting amino acid residues of PCNA seemed to be present throughout a wide region of the trimeric protein, while those of Rep appeared to be localized only in the middle part of the protein. The site-specific nicking-closing activity and the ATPase function of IMYMV Rep were impaired by PCNA. These observations lead to interesting speculations about the control of viral RCR and dynamic profiles of protein-protein interactions at the RCR origin of the geminiviruses.

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