Genetic diversity within a population of Microlaena stipoides, as revealed by AFLP markers

2014 ◽  
Vol 62 (7) ◽  
pp. 580 ◽  
Author(s):  
M. L. Mitchell ◽  
B. J. Stodart ◽  
J. M. Virgona
Keyword(s):  
Genetic Diversity ◽  
Perennial Grass ◽  
Aflp Markers ◽  
Annual Rainfall ◽  
Sexual And Asexual Reproduction ◽  
Eastern Australia ◽  
Microlaena Stipoides ◽  
Native Pastures ◽  
Clonal Spread ◽  
Vegetative Spread

Microlaena stipoides (Labill.) R.Br. (microlaena), a C3 perennial grass, is common within grazed native pastures in the high-rainfall zone (>550 mm average annual rainfall) of south-eastern Australia. It has the ability to spread via seed production or vegetatively, using both rhizomes and stolons. This experiment aimed to determine how variable a microlaena population was within a single area, with the aim of determining whether microlaena relied on seed or vegetative spread to sustain populations. Leaf samples of microlaena were collected from 85 locations, sampling two transects, within a pasture at Chiltern, in north-eastern Victoria (36°12ʹS, 146°35ʹE). The genetic diversity among samples was analysed using amplified fragment length polymorphism (AFLP) markers. We obtained 1612 fragments, using 10 primers combinations. Polymorphism for the markers ranged from 47% to 65%. These results indicated that the populations of microlaena that exist within the pasture at Chiltern are likely to have undergone some degree of outcrossing (Fst = 0.0219). It is likely that recruitment is occurring from sexual reproduction as well as via clonal spread within the microlaena population examined. This ability to use vegetative spread as well as both sexual and asexual reproduction may make populations of microlaena more resilient in the longer term.

Population biology of Microlaena stipoides in a south-eastern Australian pasture

2014 ◽  
Vol 65 (8) ◽  
pp. 767 ◽  
Author(s):  
M. L. Mitchell ◽  
J. M. Virgona ◽  
J. L. Jacobs ◽  
D. R. Kemp
Keyword(s):  
Seed Predation ◽  
Population Biology ◽  
Seedling Recruitment ◽  
Perennial Grass ◽  
Early Summer ◽  
Seed Rain ◽  
Perennial Grasses ◽  
South Eastern ◽  
Eastern Australia ◽  
Microlaena Stipoides

Microlaena (Microlaena stipoides var. stipoides (Labill.) R.Br.) is a C3 perennial grass that is native to areas of south-eastern Australia. In this region, perennial grasses are important for the grazing industries because of their extended growing season and persistence over several years. This series of experiments focused on the population biology of Microlaena by studying the phenology (when seed was set), seed rain (how much seed was produced and where it fell), seed germination, germinable seedbank, seed predation and seedling recruitment in a pasture. Experiments were conducted at Chiltern, in north-eastern Victoria, on an existing native grass pasture dominated by Microlaena. Seed yields were substantial (mean 800 seeds m–2), with seed rain occurring over December–May. Microlaena has two distinct periods of high seed rain, in early summer and in early autumn. Seed predation is high. Within a 24-h period during peak seed production, up to 30% of Microlaena seed was removed from a pasture, primarily by ants. Microlaena seedlings recruited throughout an open paddock; however, seedling density was low (5 seedlings m–2). Microlaena represented only low numbers in the seedbank (0.01–0.05% of total); hence, any seedlings of Microlaena that germinate from the seedbank would face immense competition from other species. Management strategies for Microlaena-dominant pastures need to focus on the maintenance of existing plants.

Climate variability effects on simulated pasture and animal production in the perennial pasture zone of south-eastern Australia.1. Between year variability in pasture and animal production

2003 ◽  
Vol 43 (10) ◽  
pp. 1211 ◽  
Author(s):  
S. G. Clark ◽  
E. A. Austen ◽  
T. Prance ◽  
P. D. Ball
Keyword(s):  
Climate Variability ◽  
Perennial Grass ◽  
Subterranean Clover ◽  
Animal Production ◽  
Annual Rainfall ◽  
Support Tool ◽  
South Eastern ◽  
Eastern Australia ◽  
South Eastern Australia

Climate variability is a major constraint to farming in south-eastern Australia and one that is out of the farmers' control. However, a better understanding of long-term climate variability would be beneficial for on-farm management decisions. A series of long-term simulations were undertaken with the GrassGro decision support tool to determine the effect of climate variability on pasture and animal production at 6 locations in south-eastern Australia. The simulations ran from 89 to 119 years using daily weather records from each location. All simulations were for spring-lambing flocks of medium sized Merino ewes stocked at above-average district stocking rates, grazing well-fertilised, perennial grass–subterranean clover pastures. Annual rainfall total and, in particular, the distribution of rainfall during the year, were found to be more important than other weather variables in determining the amount of pasture grown in a year. The timing of the season opening rains (autumn break) was most important. The localities varied in their responses to climate variability, particularly in the timing of the autumn break; the pasture growth response to winter rainfall; and the relationship between rainfall and animal production.

New insights into the polyploid complex Cenchrus ciliaris L. (Poaceae) show its capacity for gene flow and recombination processes despite its apomictic nature

2011 ◽  
Vol 59 (6) ◽  
pp. 543 ◽  
Author(s):  
Amina Kharrat-Souissi ◽  
Alex Baumel ◽  
Franck Torre ◽  
Marianick Juin ◽  
Sonja Siljak-Yakovlev ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Perennial Grass ◽  
Common Garden ◽  
Aflp Markers ◽  
Phenotypic Traits ◽  
Cenchrus Ciliaris ◽  
Ploidy Levels ◽  
Recombination Processes

Cenchrus ciliaris L. is a C4 perennial grass of arid lands which is under the focus of different ecological issues such as response to desertification, quality of forage grass and impacts of invasions. Here, molecular and morphological analyses of the genetic diversity of several Tunisian provenances of C. ciliaris were performed to better understand the phenotypic polymorphism of this agamospermous and polyploid grass. Ten phenotypic traits associated with productivity were measured in a common garden environment. Amplified Fragment Length Polymorphism (AFLP) markers were developed to investigate the structure of genetic diversity among and within provenances and between the three ploidy levels. Heritable phenotypic traits showed considerable differences within provenances. Surprisingly, AFLP markers revealed the existence of genotypic variations between individuals of the same sibship and a high G/N value (0.55). A neighbour-joining tree based on AFLP markers revealed three major groups; tetraploid, pentaploid and a mix of pentaploid and hexaploids. These groups do not correspond completely to the geographical origin of samples. The results underline the possibility of sexual reproduction, recombination and gene flow within and between populations of C. ciliaris. In respect with the well known dynamic nature of polyploid genomes, these results should have strong consequences for the future management of this grass for both conservation and invasion issues.

Studies of competition between Nassella trichotoma (Nees) Hack. ex Arechav. (serrated tussock) and native pastures. 2. Seedling responses

2008 ◽  
Vol 59 (3) ◽  
pp. 237 ◽  
Author(s):  
W. B. Badgery ◽  
D. R. Kemp ◽  
D. L. Michalk ◽  
W. McG. King
Keyword(s):  
Dry Matter ◽  
Seedling Recruitment ◽  
Perennial Grass ◽  
Ground Cover ◽  
Perennial Grasses ◽  
Native Grass ◽  
Eastern Australia ◽  
Native Pastures ◽  
Grass Weed ◽  
Native Perennial Grass

Native perennial grass competition can substantially reduce the invasion of Nassella trichotoma (serrated tussock), a major perennial grass weed problem in south-eastern Australia. This paper reports on a field experiment that investigated the recruitment of N. trichotoma seedlings, and determined what level of native grass competition was needed to prevent establishment in the central-west of NSW. Grasslands that maintained >2 t dry matter (DM)/ha and 100% ground cover (measured in spring) prevented N. trichotoma seedling recruitment. Relatively small amounts of perennial grass (>0.5 t DM/ha measured in spring) resulted in mortality of N. trichotoma seedlings that had recruited earlier in the year, through the next summer. Flupropanate also markedly reduced native perennial grasses and substantially increased N. trichotoma seedling establishment 12 months after application. Rotational grazing to maintain adequate levels of DM was an important management tactic that prevented N. trichotoma establishment and survival.

Genetic Diversity of 118 Sugarcane Germplasm Using AFLP Markers

2014 ◽  
Vol 40 (10) ◽  
pp. 1877
Author(s):  
Feng-Gang ZAN ◽  
Cai-Wen WU ◽  
Xue-Kuan CHEN ◽  
Pei-Fang ZHAO ◽  
Jun ZHAO ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Aflp Markers ◽  
Sugarcane Germplasm

Genetic diversity in ecotypes of Tunisian date-palm (Phoenix dactyliferaL.) assessed by AFLP markers

2007 ◽  
Vol 82 (6) ◽  
pp. 929-933 ◽  
Author(s):  
S. Rhouma ◽  
S. Zehdi-Azouzi ◽  
A. Ould Mohamed Salem ◽  
A. Rhouma ◽  
M. Marrakchi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Date Palm ◽  
Aflp Markers

Satellite derived maps of pasture growth status: association of classification with botanical composition

1997 ◽  
Vol 37 (5) ◽  
pp. 547 ◽  
Author(s):  
P. J. Vickery ◽  
M. J. Hill ◽  
G. E. Donald
Keyword(s):  
Near Infrared ◽  
Perennial Grass ◽  
Vegetation Indices ◽  
Fast Growth ◽  
Perennial Grasses ◽  
Bayesian Probability ◽  
Cumulative Probability ◽  
Botanical Composition ◽  
South Wales ◽  
Native Pastures

Summary. Spectral data from the green, red and near-infrared bands of Landsat MSS and Landsat TM satellite imagery acquired in mid-spring were classified into 3 and 6 pasture growth classes respectively. The classifications were compared with a site database of botanical composition for the Northern Tablelands of New South Wales to examine the association between spectral growth class and pasture composition. Pastures ranged in composition from unimproved native perennial grasses through semi-improved mixtures of native and naturalised grasses and legumes to highly improved temperate perennial grasses and legumes. For 3 years of MSS data, the fast growth class had a mean botanical composition of about 80% improved perennial grass and 0% native; medium growth class averaged 46% improved perennial grass and 14% native; while the slow growth class had about 60% native and 1% improved perennial grass when averaged over 3 years of MSS data. For the 6 class TM data from a single year, a predictive logistic regression of cumulative probability was developed for percentage of ‘very fast’ growth pixels and ordered 10 percentile categories of improved perennial grass or native grass. Differences in patch characteristics between classes with MSS disappeared with TM reclassified to the same 3 class level. Most probable pasture type was inferred from 3 class MSS and TM data using Bayesian probability analysis. The resulting maps were similar in general appearance but detail was better with the TM data. The pasture growth classification identified highly improved perennial grass pastures and native pastures but sensitivity to intermediate pasture types was poor. Future improvement will come from direct measurement of biophysical characteristics using vegetation indices or inversion of reflectance models.

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