scholarly journals A novel method to determine trimethylantimony concentrations in plant tissue

2016 ◽  
Vol 13 (6) ◽  
pp. 919 ◽  
Author(s):  
Adrien Mestrot ◽  
Ying Ji ◽  
Susan Tandy ◽  
Wolfgang Wilcke

Environmental contextAntimony enters the soil mostly through mining and shooting activities and can thereafter be taken up by plants. In the soil, antimony may undergo several transformations such as biomethylation, leading to the formation of trimethylantimony. Here, we measured for the first time the uptake and translocation of trimethylantimony in a plant using a new extraction and analysis method. AbstractAntimony (Sb) is a relevant pollutant that can be found in elevated concentrations in soils near Sb mines and at shooting ranges. In soils, Sb occurs as trivalent Sb, SbIII, pentavalent Sb, SbV, or trimethylantimony, TMSb ((CH3)3SbO), the latter being the result of microbial biomethylation. It is important to understand the transfer of Sb species from soil to plants to assess the role of Sb in the food chain. However, this research has historically been hampered by the lack of suitable extraction and analytical methods. In this study, we validated an efficient and reliable extraction technique using oxalic acid and ascorbic acid (72.6±1.3% of Sb extracted) as well as a high-pressure liquid chromatography–inductively coupled plasma mass spectrometry (HPLC-ICP-MS) speciation analysis method to assess the uptake of TMSb in ryegrass (Lolium perenne L.), a common pasture plant, in a hydroponics experiment. Our results show that TMSb and SbIII are not converted to other species during extraction and that TMSb is taken up by ryegrass roots and translocated to the shoots. Our study also points at specific methylation–demethylation mechanisms occurring in the plant. Moreover, an unknown Sb species was found in the shoots of TMSb-treated plants, highlighting the need for further research. These new extraction and speciation methods will enable researchers to study the soil–plant transfer of organo-Sb compounds in a reliable and consistent manner.

2016 ◽  
Vol 31 (3) ◽  
pp. 679-685 ◽  
Author(s):  
WeiJuan Yang ◽  
Zhiming Xi ◽  
Xiaoxue Zeng ◽  
Ling Fang ◽  
Wenjing Jiang ◽  
...  

A novel method for sensitively and specifically counting cancer cells using a MB-based AuNP–aptamer labelling technique and ICP-MS detection is presented in this study.


2011 ◽  
Vol 383-390 ◽  
pp. 790-795
Author(s):  
Bao Hui Li ◽  
Bao Juan Tian

A method for mercury high throughput rapid speciation analysis was built by short column capillary electrophoresis (SC-CE) coupled with inductively coupled plasma mass spectrometry (ICP-MS). A micromist nebulizer was employed to increase the nebulization efficiency and a laboratory-made removable SC-CE-ICP-MS interface on the basis of cross design was applied to alleviate buffer contamination of ICP-MS. In less than 60 s methylmercury (MeHg(I)) and inorganic mercury (Hg(II)) were separated in a 16 × 75 μm i.d. short column fused-silica capillary at 21 kV, while a mixture of 30 mmol/L boric aicd + 5% (v/v) CH3OH (pH=8.60) acted as running electrolyte. The precisions (RSD, n=5) of migration time and peak area for MeHg(I) and Hg(II) were in the range of 1.4-2.6% and 3.3-3.4%, respectively. The limits of detection (3σ) mercury species were 9.7 and 12.0 μg/L, respectively. The recoveries for Hg(II) MeHg(I) were in the range of 96-107% and 99-105%.


2014 ◽  
Vol 6 (20) ◽  
pp. 8380-8387 ◽  
Author(s):  
Zhe Nie ◽  
Lingna Zheng ◽  
Weiyue Feng ◽  
Chunli Liu

An HPLC-ICP-MS method for simultaneous speciation of selenium and iodine has been developed. Species in groundwater samples from a potential Chinese HLW repository site were determined at ultra-trace level.


Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 3017 ◽  
Author(s):  
Katarzyna Karaś ◽  
Marcin Frankowski

This paper presents the results of determination of hazardous metal (Cd, Cu, Cr, Hg, Mn, Ni, Pb, Zn) and metalloid (As, Sb) levels in toys available in the Polish market. Two independent sample preparation methods were used to determine the concentration and content of the metals and metalloids. The first one is defined by the guidelines of the EN-71 standard and undertook extraction in 0.07 mol/L HCl. This method was used to conduct speciation analysis of Cr(III) and Cr(VI), as well as for the determination of selected metals and metalloids. The second method conducted mineralization in a HNO3 and H2O2 mixture using microwave energy to determine the content of metals and metalloids. Determination of chromium forms was made using the high-performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP-MS) method, while those of metals and metalloids were made using the ICP-MS technique. Additionally, in order to determine total content of chromium in toys, an energy dispersive X-ray fluorescence spectrometer (EDX) was used. The results of the analyses showed that Cr(VI) was not detected in the toys. In general, the content of heavy metals and metalloids in the studied samples was below the migration limit set by the norm EN-71.


2014 ◽  
Vol 6 (14) ◽  
pp. 5369-5375 ◽  
Author(s):  
Chao Han ◽  
Jiannan Sun ◽  
Heyong Cheng ◽  
Jinhua Liu ◽  
Zigang Xu

This work proposed a green method for fast separation of seven iodinated forms within 7 min under the gradient elution using three aqueous mobile phases, which was highly efficient, environment-friendly and ICP-MS-compatible.


2020 ◽  
Vol 17 (3) ◽  
pp. 519-525
Author(s):  
Nguyen Thi Trang ◽  
Nguyen Thi Minh Khanh ◽  
Le Duc Manh ◽  
Vu Kim Thoa ◽  
Chu Dinh Binh ◽  
...  

Zinc is an essential trace element for many physiological function in human and animal. When bound to organic substrate, zinc is more efficiently absorbed by organisms, has a high biological activity and a low toxicity. The ability of S. cerevisiae to accumulate zinc can be used for production of a zinc-rich ingredient for functional food products. However, only a few investigations on the form of Zn in S. cerevisiae have been reported. In this study, organic and inorganic compounds of zinc in yeast extract samples was separated on D101 macro-resin and quantified by inductively coupled plasma mass spectrometry (ICP-MS). Inorganic and organic compounds of zinc in yeast samples were extracted with photphate buffer by bead mills method and fractionated on the D101 macro-resin. All critical parameters of extraction conditions as well as separation conditions of zinc compounds on D101 macro-resin were investigated and optimized. In addition, speciation analysis of zinc compounds also was performed with online high-performance ion chromatography in combination with inductively coupled plasma mass spectrometry (HPIC-ICP-MS). Analyzed results indicated that the content of the organic state of Zn was more than that of the inorganic state. Organic zinc was the most abundance in yeast samples in the 51.56 – 88.17%. The highest organic zinc was found in S. cerevisiae A112 at 88.17%. In all of the samples, the organic zinc was found in the polysaccharide fraction was more than that of protein fraction. Our research results are significant for medical and food applications. Speciation analysis of trace element Zn is helpful to elucidate its pharmacological mechanism.


2010 ◽  
Vol 82 (2) ◽  
pp. 447-460 ◽  
Author(s):  
Alfredo Sanz-Medel ◽  
Maria Luisa Fernández-Sánchez ◽  
Héctor González Iglesias ◽  
José Blas López-Sastre

Human breast milk can be considered as “ideal” food for the correct development of newborn babies and, for those that are not breast-fed, formula milk has to be used instead. Ideally, the composition of such formula milk preparations should closely resemble that of maternal human milk. Considerable differences between both in the total content of trace elements such as Fe, Cu, Se, Zn, and I and in their chemical form in both milk types have been demonstrated. Speciation analysis in milk whey was carried out first by high-performance liquid chromatography (HPLC) with inductively coupled plasma-mass spectrometry (ICP-MS) elemental detection and showed that the observed element distribution patterns were very different in the investigated human and formula milks. Using complementary molecular mass techniques (i.e., MALDI-TOF), the identity and chemical characterization of some biomolecules (e.g., protein) with which metals are associated in each fraction was also established (by a typical heteroatom-tagged proteomics protocol). Attempts to assess the nutritional value of elemental supplements in formula milk with the aid of quantitative chemical speciation, using stable isotopes in combination with ICP-MS and isotope pattern deconvolution (IPD), proved to be successful to differentiate and quantify endogenous (natural) and exogenous (supplemented) Se or Fe trace levels. In particular, the application of such ICP-MS based techniques to study Se bioavailability from formula milk and metabolism in Se-supplemented lactating rats is discussed in detail. Quantification of selenospecies of endogenous (natural) and exogenous (supplement) Se in rat’s urine is demonstrated and relevant information on possible Se biotransformations and its final catabolism from such results is discussed.


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