Comparative transcriptional profiling of two rice genotypes carrying SUB1A-1 but exhibiting differential tolerance to submergence

2012 ◽  
Vol 39 (6) ◽  
pp. 449 ◽  
Author(s):  
Huaiyang Xiong ◽  
Yang Li ◽  
Jing Yang ◽  
Yangsheng Li
Keyword(s):  
Oryza Sativa L ◽  
Indica Rice ◽  
Transcriptional Profiling ◽  
Submergence Tolerance ◽  
Antioxidant Genes ◽  
Carbohydrate Consumption ◽  
Rainfed Lowland ◽  
Important Trait ◽  
Rice Genotypes ◽  
And Control

Submergence tolerance in rainfed lowland rice (Oryza sativa L.) is determined mainly by SUB1A-1, which confers the tolerance by regulating the ethylene- and gibberellin-mediated gene expression responsible for carbohydrate consumption, cell elongation and ethanolic fermentation. However, two indica rice genotypes, FR13A and Goda Heenati, both carrying this gene, exhibited differential tolerance to submergence. Comparative analysis of transcriptional profiling of the two genotypes revealed that many of antioxidant genes were more highly expressed in FR13A than in Goda Heenati under both submergence and control conditions, or only under submergence, whereas most of genes involved in biosynthesis and signalling of ethylene and GA and in anaerobic carbohydrate metabolism had comparable levels of expression between genotypes under the same conditions. H2O2 and malondialdehyde (MDA) assays demonstrated that Goda Heenati accumulated more H2O2 and had more MDA, a product of lipid peroxidation, than FR13A under submergence. These findings suggest that apart from SUB1A-mediated ‘quiescence strategy’, the detoxification of reactive oxygen species (ROS) is another important trait associated with submergence tolerance. The information obtained from this study helps in further understanding of the mechanism underlying submergence tolerance.

scholarly journals Pattern of genetic variation in rice (Oryza sativa L.) population for sheath blight resistance over the seasons

2021 ◽  
Vol 81 (01) ◽  
pp. 132-134
Author(s):  
R.* Naveenkumar ◽  
Vineeta Singh ◽  
P. K. Singh ◽  
A. Anandan
Keyword(s):  
Coefficient Of Variation ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Sheath Blight ◽  
Tiller Number ◽  
Genetic Advance ◽  
Panicle Length ◽  
Progress Curve ◽  
Rice Genotypes ◽  
Cent Disease

A set of 192 indica rice accessions were evaluated in two seasons to generate information on the extent of genetic variability, broad-sense heritability and genetic advance for yield and sheath blight related traits. The estimated genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) varied over the season. Largely, PCV values were relatively higher than GCV with reference to the studied parameters. However, GCV was found to be medium for per cent disease index (PDI) of 14th day, 28th day, area under the disease progress curve (AUDPC), and tiller number over the seasons. Interestingly, grain yield was found to be high. The trait plant height and panicle length had moderate heritability coupled with moderate genetic advance, whereas AUDPC was moderately heritable coupled with high genetic advance. The findings may be useful in breeding improved rice genotypes.

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

scholarly journals Improved Transformation and Regeneration of Indica Rice: Disruption of SUB1A as a Test Case via CRISPR-Cas9

2021 ◽  
Vol 22 (13) ◽  
pp. 6989
Author(s):  
Yuya Liang ◽  
Sudip Biswas ◽  
Backki Kim ◽  
Julia Bailey-Serres ◽  
Endang M. Septiningsih
Keyword(s):  
Gene Editing ◽  
Indica Rice ◽  
Crop Improvement ◽  
Immature Embryo ◽  
Test Case ◽  
Submergence Tolerance ◽  
Embryogenic Calli ◽  
Submergence Stress ◽  
Efficient Vector ◽  
Vector Delivery

Gene editing by use of clustered regularly interspaced short palindromic repeats (CRISPR) has become a powerful tool for crop improvement. However, a common bottleneck in the application of this approach to grain crops, including rice (Oryza sativa), is efficient vector delivery and calli regeneration, which can be hampered by genotype-dependent requirements for plant regeneration. Here, methods for Agrobacterium-mediated and biolistic transformation and regeneration of indica rice were optimized using CRISPR-Cas9 gene-editing of the submergence tolerance regulator SUBMERGENCE 1A-1 gene of the cultivar Ciherang-Sub1. Callus induction and plantlet regeneration methods were optimized for embryogenic calli derived from immature embryos and mature seed-derived calli. Optimized regeneration (95%) and maximal editing efficiency (100%) were obtained from the immature embryo-derived calli. Phenotyping of T1 seeds derived from the edited T0 plants under submergence stress demonstrated inferior phenotype compared to their controls, which phenotypically validates the disruption of SUB1A-1 function. The methods pave the way for rapid CRISPR-Cas9 gene editing of recalcitrant indica rice cultivars.

scholarly journals Comparative Transcriptional Profiling of Contrasting Rice Genotypes Shows Expression Differences during Arsenic Stress

2015 ◽  
Vol 8 (2) ◽  
Author(s):  
Arti Rai ◽  
Archana Bhardwaj ◽  
Prashant Misra ◽  
Sumit K. Bag ◽  
Bijan Adhikari ◽  
...  
Keyword(s):  
Transcriptional Profiling ◽  
Arsenic Stress ◽  
Rice Genotypes
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