Comparative Transcriptional Profiling of Contrasting Rice Genotypes Shows Expression Differences during Arsenic Stress

Submergence tolerance in rainfed lowland rice (Oryza sativa L.) is determined mainly by SUB1A-1, which confers the tolerance by regulating the ethylene- and gibberellin-mediated gene expression responsible for carbohydrate consumption, cell elongation and ethanolic fermentation. However, two indica rice genotypes, FR13A and Goda Heenati, both carrying this gene, exhibited differential tolerance to submergence. Comparative analysis of transcriptional profiling of the two genotypes revealed that many of antioxidant genes were more highly expressed in FR13A than in Goda Heenati under both submergence and control conditions, or only under submergence, whereas most of genes involved in biosynthesis and signalling of ethylene and GA and in anaerobic carbohydrate metabolism had comparable levels of expression between genotypes under the same conditions. H2O2 and malondialdehyde (MDA) assays demonstrated that Goda Heenati accumulated more H2O2 and had more MDA, a product of lipid peroxidation, than FR13A under submergence. These findings suggest that apart from SUB1A-mediated ‘quiescence strategy’, the detoxification of reactive oxygen species (ROS) is another important trait associated with submergence tolerance. The information obtained from this study helps in further understanding of the mechanism underlying submergence tolerance.

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642: Novel Prediction of Chemosensitivity in Urothelial Carcinoma by Transcriptional Profiling

The Journal of Urology ◽

10.1016/s0022-5347(18)32888-x ◽

2006 ◽

Vol 175 (4S) ◽

pp. 206-207

Author(s):

Dmytro M. Havaleshko ◽

Hyungjun Cho ◽

Mark Conaway ◽

Jae K. Lee ◽

Dan Theodorescu

Keyword(s):

Urothelial Carcinoma ◽

Transcriptional Profiling

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Phosphorus fertilization in inbred and hybrid rice

Dhaka University Journal of Biological Sciences ◽

10.3329/dujbs.v19i2.8962 ◽

1970 ◽

Vol 19 (2) ◽

pp. 181-187

Author(s):

MR Islam ◽

PK Saha ◽

SK Zaman ◽

MJ Uddin

Keyword(s):

Grain Yield ◽

Key Words ◽

Hybrid Rice ◽

Phosphorus Fertilization ◽

Phosphorus Fertilizer ◽

Yield Difference ◽

P Balance ◽

Rice Genotypes ◽

Boro Rice

Five phosphorus rates (0, 5, 10, 20 and 30 kg P/ha) were tested with four rice genotypes in Boro (BRRI dhan36, BRRI dhan45, EH1 and EH2) and T. Aman (BRRI dhan30, BRRI dhan49, EH1 and EH2) season. Phosphorus rates did not influence grain yield irrespective of varieties in T. Aman season while in Boro season P response was observed among the P rates. Application of P @ 10 kg/ha significantly increased the grain yield. But when P was applied @ 20 and 30 kg P/ha, the grain yield difference was not significant. The optimum and economic rate of P for T. Aman was 20 kg P/ha but in Boro rice the optimum and economic doses of P were 22 and 30 kg/ha, respectively. Hybrid entries (EH1 and EH2) used P more efficiently than inbred varieties. A negative P balance was observed up to 10 kg P/ha. Key words: Response; Phosphorus fertilizer; Inbred; Hybrid rice DOI: http://dx.doi.org/10.3329/dujbs.v19i2.8962 DUJBS 2010; 19(2): 181-187

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Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

Progressive Agriculture ◽

10.3329/pa.v20i1-2.16839 ◽

2013 ◽

Vol 20 (1-2) ◽

pp. 1-8

Author(s):

MM Rahman ◽

L Rahman ◽

SN Begum ◽

F Nur

Keyword(s):

Genetic Distance ◽

Gene Diversity ◽

Random Amplified Polymorphic Dna ◽

Molecular Genetic ◽

Molecular Genetic Analysis ◽

Polymorphic Loci ◽

Rice Genotypes ◽

High Level ◽

Genetic Diversity Study ◽

Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Neis genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 8, 2009

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Screening of rice genotypes for resistance to the brown planthopper, Nilaparvata lugens Stål

Cereal Research Communications ◽

10.1556/crc.2012.0001 ◽

2012 ◽

Vol 40 (4) ◽

pp. 502-508 ◽

Cited By ~ 1

Author(s):

M. P. Ali ◽

Salem S. Alghamdi ◽

M. A. Begum ◽

A. B. M. Anwar Uddin ◽

M. Z. Alam ◽

...

Keyword(s):

Brown Planthopper ◽

Nilaparvata Lugens ◽

Rice Genotypes

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Transcriptional profiling of iPSC-derived neurons with reciprocal monogenic CNV in 3p26.3 region

Nauchno-prakticheskii zhurnal «Medicinskaia genetika» ◽

10.25557/2073-7998.2020.03.10-11 ◽

2020 ◽

pp. 10-11

Author(s):

М.Е. Лопаткина ◽

В.С. Фишман ◽

М.М. Гридина ◽

Н.А. Скрябин ◽

Т.В. Никитина ◽

...

Keyword(s):

Gene Expression ◽

Copy Number ◽

System Development ◽

Transcriptional Profiling ◽

Global Gene Expression ◽

Nervous System Development ◽

Number Variation ◽

The Central Nervous System ◽

Cntn6 Gene ◽

Copy Number Changes

Проведен анализ генной экспрессии в нейронах, дифференцированных из индуцированных плюрипотентных стволовых клеток пациентов с идиопатическими интеллектуальными нарушениями и реципрокными хромосомными мутациями в регионе 3p26.3, затрагивающими единственный ген CNTN6. Для нейронов с различным типом хромосомных аберраций была показана глобальная дисрегуляция генной экспрессии. В нейронах с вариациями числа копий гена CNTN6 была снижена экспрессия генов, продукты которых вовлечены в процессы развития центральной нервной системы. The gene expression analysis of iPSC-derived neurons, obtained from patients with idiopathic intellectual disability and reciprocal microdeletion and microduplication in 3p26.3 region affecting the single CNTN6 gene was performed. The global gene expression dysregulation was demonstrated for cells with CNTN6 copy number variation. Gene expression in neurons with CNTN6 copy number changes was downregulated for genes, whose products are involved in the central nervous system development.

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