Molecular taxonomy of the Anadenobolus excisus (Diplopoda : Spirobolida : Rhinocricidae) species-group on the Caribbean island of Jamaica

2003 ◽  
Vol 17 (4) ◽  
pp. 515 ◽  
Author(s):  
Jason E. Bond ◽  
Petra Sierwald
Keyword(s):  
Molecular Taxonomy ◽  
Molecular Data ◽  
Species Group ◽  
Morphological Data ◽  
Rrna Gene ◽  
Nominal Species ◽  
Caribbean Island ◽  
The Caribbean ◽  
The 16S Rrna Gene ◽  
Mtdna Genealogy

This paper documents the mtDNA genealogy and molecular taxonomy of the Anadenobolus excisus millipede species-group on the island of Jamaica. This endemic species-group originally comprised two nominal species, A. excisus (Karsch) and A. holomelanus Pocock. However, the latter species was considered by Hoffman likely to be a subspecies of the former, owing to their overall morphological and gonopodal similarity (the secondary sexual features most commonly used to delineate millipede species). We summarise molecular and morphological data that paints a rather different picture of the diversity in this group. Based on the 16S rRNA gene of the mitochondrion and a comparative analysis of millipede size (reported here and elsewhere), we find that this species-group comprises at least three sibling species, one of which, A. dissimulans, sp. nov., is newly described. The study documents the first myriapod species diagnosed on the basis of molecular data.

Phylogenetic systematics of day geckos, genus Phelsuma, based on molecular and morphological data (Squamata: Gekkonidae)

Zootaxa ◽  
2010 ◽  
Vol 2429 (1) ◽  
pp. 1 ◽  
Author(s):  
SARAH ROCHA ◽  
HERBERT RÖSLER ◽  
PHILIP-SEBASTIAN GEHRING ◽  
FRANK GLAW ◽  
DAVID POSADA ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Species Assemblages ◽  
Rrna Gene ◽  
Phylogenetic Systematics ◽  
Multigene Phylogeny ◽  
Species Groups ◽  
The 16S Rrna Gene

We review the intrageneric systematics of day geckos, genus Phelsuma (Squamata: Sauria: Gekkonidae) in the light of a recently published multigene phylogeny, and provide an overview of 19 selected chromatic, morphological and behavioural characters for all currently recognized species and subspecies in the genus. To replace previous attempts of dividing the genus into phenetic species assemblages, we propose the following monophyletic species groups and summarize the morphological characters that partly support this grouping: Phelsuma barbouri group (P. barbouri, P. pronki); P. dubia group (P. berghofi, P. dubia, P. flavigularis, P. hielscheri, P. malamakibo, P. modesta, P. nigristriata, P. ravenala); P. mutabilis group (P. borai, P. breviceps, P. mutabilis); P. cepediana group (P. borbonica, P. cepediana, P. guentheri, P. guimbeaui, P. inexpectata, P. ornata, P. rosagularis as well as the extinct species P. edwardnewtonii and P. gigas); P. astriata group (P. astriata, P. sundbergi); P. madagascariensis group (P. abbotti, P. grandis, P. guttata, P. kochi, P. madagascariensis, P. parkeri, P. seippi and probably P. masohoala); P. lineata group (P. antanosy, P. comorensis, P. kely, P. lineata, P. pusilla, P. quadriocellata and probably P. hoeschi); P. laticauda group (P. laticauda, P. pasteuri, P. robertmertensi, P. v-nigra); P. klemmeri group (P. klemmeri); P. andamanense group (P. andamanense) and P. standingi group (P. standingi). Due to their equivocal placements based on different molecular markers, we refrain from assigning P. serraticauda and P. vanheygeni to any of these species groups at this time. We complement the molecular data with new trees based on partial sequences of the 16S rRNA gene for the P. madagascariensis- and P. lineata groups and propose to elevate P. lineata dorsivittata and P. quadriocellata parva to full species rank as P. dorsivittata and P. parva.

Revision of Autolytinae (Syllidae: Polychaeta)

Zootaxa ◽  
2004 ◽  
Vol 680 (1) ◽  
pp. 1 ◽  
Author(s):  
ARNE NYGREN
Keyword(s):  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Sets ◽  
Nominal Species ◽  
Strict Consensus Tree ◽  
Incertae Sedis ◽  
Nomina Dubia ◽  
Morphological And Molecular Data ◽  
Combined Data

Autolytinae is revised based on available types, and newly collected specimens. Out of 170 nominal species, 18 are considered as incertae sedis, 43 are regarded as junior synonyms, and 25 are referred to as nomina dubia. The relationships of Autolytinae is assessed from 51 morphological characters and 211 states for 76 ingroup-taxa, and 460 molecular characters from mitochondrial 16S rDNA and nuclear 18S rDNA for 31 ingroup-taxa; outgroups include 12 non-autolytine syllid polychaetes. Two analyses are provided, one including morphological data only, and one with combined morphological and molecular data sets. The resulting strict consensus tree from the combined data is chosen for a reclassification. Three main clades are identified: Procerini trib. n., Autolytini Grube, 1850, and Epigamia gen. n. Proceraea Ehlers, 1864 and Myrianida Milne Edwards, 1845 are referred to as nomen protectum, while Scolopendra Slabber, 1781, Podonereis Blainville, 1818, Amytis Savigny, 1822, Polynice Savigny, 1822, and Nereisyllis Blainville, 1828 are considered

The genus Cystoseira s.l. (Ochrophyta, Fucales, Sargassaceae) in the Black Sea: morphological variability and molecular taxonomy of Gongolaria barbata and endemic Ericaria crinita f. bosphorica comb. nov.

Phytotaxa ◽  
2021 ◽  
Vol 480 (1) ◽  
pp. 1-21
Author(s):  
SOFIA S. SADOGURSKA ◽  
JOÃO NEIVA ◽  
ANNALISA FALACE ◽  
ESTER A. SERRÃO ◽  
ÁLVARO ISRAEL
Keyword(s):  
Mediterranean Sea ◽  
Black Sea ◽  
Molecular Taxonomy ◽  
Morphological Variability ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Morphological Data ◽  
The Black Sea ◽  
Morphological Identification ◽  
Additional Tool

Brown algae of the genus Cystoseira sensu lato form the most diverse and productive marine ecosystems throughout the Mediterranean Sea and have equal roles also in the Black Sea where they have been decreasing in the recent years. The taxonomy of Cystoseira s.l. taxa from the Black Sea is still not well understood, and questions arise when related taxa have to be delimited. In addition to morphological descriptions, this study provides for the first time molecular data of the Black Sea Cystoseira s.l. distinct morphologies as an additional tool to clarify their identities and phylogenetic affinities. The analysis of two mitochondrial markers (cytochrome oxidase subunit 1—COI, and 23S-tRNAVal intergenic spacer—mt-spacer) showed that Cystoseira s.l. specimens from the Black Sea belong to two recently resurrected genera, namely Gongolaria and Ericaria. Molecular data confirm the morphological identification of G. barbata, which is characterized by high morphological plasticity in the Black Sea. The morphological data presented in this study support the transition of G. barbata to the genus Gongolaria, which was previously proposed based solely on genetic data. For the Black Sea endemic taxon C. bosphorica, sequence divergence suggests conspecificity with Mediterranean Sea species E. crinita and E. barbatula. However, considering original morphological characteristics of the taxon, its geographical isolation, and endemism, the new combination Ericaria crinita f. bosphorica comb. nov. is proposed.

Two additional treefrogs of the Boophis ulftunni species group (Anura: Mantellidae) discovered in rainforests of northern and south-eastern Madagascar

Zootaxa ◽  
2008 ◽  
Vol 1814 (1) ◽  
pp. 37 ◽  
Author(s):  
JÖRN KÖHLER ◽  
FRANK GLAW ◽  
MIGUEL VENCES
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Ovigerous Female ◽  
Species Group ◽  
Rrna Gene ◽  
Threat Status ◽  
Frog Species ◽  
Southern Central ◽  
Phylogenetic Affinities ◽  
The 16S Rrna Gene

We describe two new frog species of the endemic Malagasy-Comoroan genus Boophis. One species, described as Boophis baetkei sp. n., originates from Forêt d'Ambre Special Reserve in northernmost Madagascar, whereas Boophis lilianae sp. n. was discovered near Ifanadiana and Ranomafana in the Southern Central East of the island. Both new species have very deep genetic divergences in the 16S rRNA gene that complicate the assessment of their phylogenetic affinities but are here tentatively assigned to the recently defined Boophis ulftunni species group based on phenetic similarity and preliminary results of analyses of other genes. All three species known in this group share a green dorsum with translucent shade in life, a pigmented venter and, most characteristic, pink markings in life and in preservative. Boophis lilianae sp. n. is the smallest species of Boophis known so far (SVL of adult male 18.3 mm, ovigerous female 20.0 mm). Phylogenetic relationships, distribution and threat status of the new species are discussed.

Phylogenetic analysis of the Neotropical Pristimantis leptolophus species group (Anura: Craugastoridae): molecular approach and description of a new polymorphic species 

Zootaxa ◽  
2017 ◽  
Vol 4242 (2) ◽  
pp. 313
Author(s):  
MAURICIO RIVERA-CORREA ◽  
CARLOS JIMÉNEZ-RIVILLAS ◽  
JUAN M. DAZA
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Reconstruction ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Species Group ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
Molecular Evidence ◽  
Polymorphic Species ◽  
Species Groups

Pristimantis, distributed throughout the New World tropics, is the most speciose vertebrate genus. Pristimantis presents an enormous morphological diversity and is currently divided into several demonstrably non-monophyletic phenetic species groups. With the purpose of increasing our understanding of Pristimantis systematics, we present the first phylogenetic analysis using molecular evidence to test the monophyly and infer evolutionary relationships within the Pristimantis leptolophus group, an endemic group of frogs from the highlands of the Colombian Andes. Our phylogenetic reconstruction recovers the group as monophyletic with high support, indicating general concordance between molecular data and morphological data. In addition, we describe a new polymorphic species lacking conspicuous tubercles, a regular attribute among species of the P. leptolophus species group and endemic from the Páramo de Sonsón complex (Antioquia, Colombia). The phylogenetic position of the new species is inferred and other systematic implications in the light of our results are discussed. 

Phylogenetic relationships within Ovulidae (Gastropoda: Cypraeoidea) based on molecular data from the 16S rRNA gene

2005 ◽  
Vol 147 (2) ◽  
pp. 411-420 ◽  
Author(s):  
S. Schiaparelli ◽  
M. Barucca ◽  
E. Olmo ◽  
M. Boyer ◽  
A. Canapa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Relationships ◽  
Molecular Data ◽  
Rrna Gene ◽  
The 16S Rrna Gene

scholarly journals Species boundaries among extremely diverse and sexually dimorphic Arrenurus water mites (Acariformes: Hydrachnidiae: Arrenuridae)

2021 ◽  
Author(s):  
Mariusz Wiecek ◽  
Lukasz Broda ◽  
Heather Proctor ◽  
Miroslawa Dabert ◽  
Bruce P. Smith ◽  
...  
Keyword(s):  
Molecular Taxonomy ◽  
Molecular Data ◽  
Species Boundaries ◽  
Rrna Gene ◽  
Species Variation ◽  
28S Rrna ◽  
Water Mites ◽  
Running Water ◽  
Reproductive Morphology ◽  
Molecular Approaches

Arrenurus (Arrenuridae) is the most species-rich genus of mites with about 950 named species that inhabit standing, and to a lesser extent, running water habitats around the world. To date, distinguishing species of Arrenurus has been based on male reproductive morphology. Here, we use morphological and molecular approaches to examine species boundaries among 42 named species of Arrenurus, including four named species that have colour variants (red and green A. americanus, and red and blue A. intermedius, A. manubriator and A. apetiolatus), and two unnamed morphospecies. In this study, we examine male genital structures with the use of SEM techniques, and apply mitochondrial (COI barcode region) and nuclear (28S rRNA) gene fragments to test whether male morphology reflects species boundaries in Arrenurus assessed by molecular analyses. Our results reveal that male reproductive morphology parallels species boundaries as judged by molecular data. We discuss the cases of genetically poorly diversified, yet morphologically clearly defined named species. Moreover, we show that based on the species we examined, colour morphs within otherwise morphologically similar specimens represent within-species variation and, in the absence of other diagnostic traits, colour itself can be misleading in distinguishing species. Our outcomes on molecular taxonomy of Arrenurus provide a background for testing hypotheses about speciation rate in water mites.

scholarly journals DNA-barcoding of forensically important blow flies (Diptera: Calliphoridae) in the Caribbean Region

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3516 ◽  
Author(s):  
Sohath Z. Yusseff-Vanegas ◽  
Ingi Agnarsson
Keyword(s):  
Dna Barcoding ◽  
Species Delimitation ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Morphological Data ◽  
Caribbean Region ◽  
Reference Database ◽  
Correct Identification ◽  
Accurate Identification ◽  
The Caribbean

Correct identification of forensically important insects, such as flies in the family Calliphoridae, is a crucial step for them to be used as evidence in legal investigations. Traditional identification based on morphology has been effective, but has some limitations when it comes to identifying immature stages of certain species. DNA-barcoding, using COI, has demonstrated potential for rapid and accurate identification of Calliphoridae, however, this gene does not reliably distinguish among some recently diverged species, raising questions about its use for delimitation of species of forensic importance. To facilitate DNA based identification of Calliphoridae in the Caribbean we developed a vouchered reference collection from across the region, and a DNA sequence database, and further added the nuclear ITS2 as a second marker to increase accuracy of identification through barcoding. We morphologically identified freshly collected specimens, did phylogenetic analyses and employed several species delimitation methods for a total of 468 individuals representing 19 described species. Our results show that combination of COI + ITS2 genes yields more accurate identification and diagnoses, and better agreement with morphological data, than the mitochondrial barcodes alone. All of our results from independent and concatenated trees and most of the species delimitation methods yield considerably higher diversity estimates than the distance based approach and morphology. Molecular data support at least 24 distinct clades within Calliphoridae in this study, recovering substantial geographic variation forLucilia eximia, Lucilia retroversa, Lucilia ricaandChloroprocta idioidea, probably indicating several cryptic species. In sum, our study demonstrates the importance of employing a second nuclear marker for barcoding analyses and species delimitation of calliphorids, and the power of molecular data in combination with a complete reference database to enable identification of taxonomically and geographically diverse insects of forensic importance.

Conflict and congruence between morphological and molecular data: revision of the Merodon constans group (Diptera : Syrphidae)

2020 ◽  
Author(s):  
Ante Vujić ◽  
Snežana Radenković ◽  
Laura Likov ◽  
Andrijana Andrić ◽  
Marina Janković ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Distinct Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Species Group ◽  
Rrna Genes ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Status ◽  
Data Revision

We revise the Merodon constans species group of the genus Merodon Meigen, 1803 (Diptera: Syrphidae), provide morphological diagnosesand descriptions, as well as an illustrated key and a discussion on the different taxonomic characters used. In total, 15 species were studied, their geographic distributions are presented on maps, and nine new species are described. Two species are redefined and neotypes are designated, lectotypes are designated for five species, and onespeciesis reinstated as valid. Following a detailed study of type material in different entomological collections, the status of several species is revised and three new synonymies are proposed. The M. constans species group was resolved as being monophyletic within the M. albifrons lineage based on molecular analyses using COI and 28S rRNA gene sequences. Three species morphologically similar to M. constans (Rossi, 1794) but occurring outside its distributional rangewere supported as being valid and distinct species on the basis of molecular data, but they were not distinguishable based on morphological characters. By contrast, continental populations of M. analis Meigen, 1822 could not be separated from Mediterranean M. constans based on differences in COI or 28S rRNA genes. The same molecular markers could not discriminate between two other species pairs. We conclude that these molecular markers only partially resolve species within the M. constans group. Geometric morphometry of wing shape successfully separated M. analis and M. constans, as well as M. spineus Vujić, Šašić Zorić & Likov, sp. nov. in both species and population analyses.

A new leaf tailed gecko species from northern Madagascar with a preliminary assessment of molecular and morphological variability in the Uroplatus ebenaui group

Zootaxa ◽  
2011 ◽  
Vol 3022 (1) ◽  
pp. 39 ◽  
Author(s):  
FANOMEZANA MIHAJA RATSOAVINA ◽  
EDWARD E. LOUIS JR. ◽  
ANGELICA CROTTINI ◽  
ROGER-DANIEL RANDRIANIAINA ◽  
FRANK GLAW ◽  
...  
Keyword(s):  
New Species ◽  
Morphological Variability ◽  
Molecular Data ◽  
Species Group ◽  
Genetic Admixture ◽  
Character State ◽  
Nominal Species ◽  
Data Set ◽  
Candidate Species ◽  
High Level

Endemic to Madagascar, the genus Uroplatus of the family Gekkonidae consists of 13 nominal species of leaf-tailed geckos. These forest dwelling lizards are famous for their cryptic and odd appearance. We describe a new species of the Uroplatus ebenaui group from the Montagne d’Ambre massif in northern Madagascar. Uroplatus finiavana sp. nov., is morphologically similar to the sympatric U. ebenaui but differs in multiple character state expressions, among which are a longer tail and an unpigmentated oral mucosa. It also can be differentiated from U. ebenaui and all other Uroplatus species based on a high level of divergence in the mitochondrial ND4 gene and the nuclear C-mos gene, and no instances of haplotype sharing exist in these genes among the analysed species. The new species is relatively abundant at Montagne d'Ambre National Park where at lower elevations (ca. 700 m) it occurs together with U. ebenaui, without any signal of genetic admixture. Records of U. ebenaui in the mountains and forest blocks of northern Madagascar (especially in the Tsaratanana massif) actually represent other candidate species with distinct morphology and characterized by a high genetic divergence from the described species. Beside the description of the new species we discuss the geographic provenance of the holotypes of the nominal species and synonyms in the U. ebenaui group and provide further information on the phylogeny of the U. ebenaui species group including the first incorporation of Uroplatus malama in a molecular data set.

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