Phylogenetic systematics of day geckos, genus Phelsuma, based on molecular and morphological data (Squamata: Gekkonidae)

Zootaxa ◽  
2010 ◽  
Vol 2429 (1) ◽  
pp. 1 ◽  
Author(s):  
SARAH ROCHA ◽  
HERBERT RÖSLER ◽  
PHILIP-SEBASTIAN GEHRING ◽  
FRANK GLAW ◽  
DAVID POSADA ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Species Assemblages ◽  
Rrna Gene ◽  
Phylogenetic Systematics ◽  
Multigene Phylogeny ◽  
Species Groups ◽  
The 16S Rrna Gene

We review the intrageneric systematics of day geckos, genus Phelsuma (Squamata: Sauria: Gekkonidae) in the light of a recently published multigene phylogeny, and provide an overview of 19 selected chromatic, morphological and behavioural characters for all currently recognized species and subspecies in the genus. To replace previous attempts of dividing the genus into phenetic species assemblages, we propose the following monophyletic species groups and summarize the morphological characters that partly support this grouping: Phelsuma barbouri group (P. barbouri, P. pronki); P. dubia group (P. berghofi, P. dubia, P. flavigularis, P. hielscheri, P. malamakibo, P. modesta, P. nigristriata, P. ravenala); P. mutabilis group (P. borai, P. breviceps, P. mutabilis); P. cepediana group (P. borbonica, P. cepediana, P. guentheri, P. guimbeaui, P. inexpectata, P. ornata, P. rosagularis as well as the extinct species P. edwardnewtonii and P. gigas); P. astriata group (P. astriata, P. sundbergi); P. madagascariensis group (P. abbotti, P. grandis, P. guttata, P. kochi, P. madagascariensis, P. parkeri, P. seippi and probably P. masohoala); P. lineata group (P. antanosy, P. comorensis, P. kely, P. lineata, P. pusilla, P. quadriocellata and probably P. hoeschi); P. laticauda group (P. laticauda, P. pasteuri, P. robertmertensi, P. v-nigra); P. klemmeri group (P. klemmeri); P. andamanense group (P. andamanense) and P. standingi group (P. standingi). Due to their equivocal placements based on different molecular markers, we refrain from assigning P. serraticauda and P. vanheygeni to any of these species groups at this time. We complement the molecular data with new trees based on partial sequences of the 16S rRNA gene for the P. madagascariensis- and P. lineata groups and propose to elevate P. lineata dorsivittata and P. quadriocellata parva to full species rank as P. dorsivittata and P. parva.

Conflict and congruence between morphological and molecular data: revision of the Merodon constans group (Diptera : Syrphidae)

2020 ◽  
Author(s):  
Ante Vujić ◽  
Snežana Radenković ◽  
Laura Likov ◽  
Andrijana Andrić ◽  
Marina Janković ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Distinct Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Species Group ◽  
Rrna Genes ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Status ◽  
Data Revision

We revise the Merodon constans species group of the genus Merodon Meigen, 1803 (Diptera: Syrphidae), provide morphological diagnosesand descriptions, as well as an illustrated key and a discussion on the different taxonomic characters used. In total, 15 species were studied, their geographic distributions are presented on maps, and nine new species are described. Two species are redefined and neotypes are designated, lectotypes are designated for five species, and onespeciesis reinstated as valid. Following a detailed study of type material in different entomological collections, the status of several species is revised and three new synonymies are proposed. The M. constans species group was resolved as being monophyletic within the M. albifrons lineage based on molecular analyses using COI and 28S rRNA gene sequences. Three species morphologically similar to M. constans (Rossi, 1794) but occurring outside its distributional rangewere supported as being valid and distinct species on the basis of molecular data, but they were not distinguishable based on morphological characters. By contrast, continental populations of M. analis Meigen, 1822 could not be separated from Mediterranean M. constans based on differences in COI or 28S rRNA genes. The same molecular markers could not discriminate between two other species pairs. We conclude that these molecular markers only partially resolve species within the M. constans group. Geometric morphometry of wing shape successfully separated M. analis and M. constans, as well as M. spineus Vujić, Šašić Zorić & Likov, sp. nov. in both species and population analyses.

Molecular taxonomy of the Anadenobolus excisus (Diplopoda : Spirobolida : Rhinocricidae) species-group on the Caribbean island of Jamaica

2003 ◽  
Vol 17 (4) ◽  
pp. 515 ◽  
Author(s):  
Jason E. Bond ◽  
Petra Sierwald
Keyword(s):  
Molecular Taxonomy ◽  
Molecular Data ◽  
Species Group ◽  
Morphological Data ◽  
Rrna Gene ◽  
Nominal Species ◽  
Caribbean Island ◽  
The Caribbean ◽  
The 16S Rrna Gene ◽  
Mtdna Genealogy

This paper documents the mtDNA genealogy and molecular taxonomy of the Anadenobolus excisus millipede species-group on the island of Jamaica. This endemic species-group originally comprised two nominal species, A. excisus (Karsch) and A. holomelanus Pocock. However, the latter species was considered by Hoffman likely to be a subspecies of the former, owing to their overall morphological and gonopodal similarity (the secondary sexual features most commonly used to delineate millipede species). We summarise molecular and morphological data that paints a rather different picture of the diversity in this group. Based on the 16S rRNA gene of the mitochondrion and a comparative analysis of millipede size (reported here and elsewhere), we find that this species-group comprises at least three sibling species, one of which, A. dissimulans, sp. nov., is newly described. The study documents the first myriapod species diagnosed on the basis of molecular data.

Comparison between molecular and morphological-based phylogenies of galerucine/alticine leaf beetles (Coleoptera: Chrysomelidae)

2003 ◽  
Vol 34 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Karl Kjer ◽  
Sung Jin Kim ◽  
Catherine Duckett
Keyword(s):  
Molecular Markers ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Combined Analysis ◽  
Leaf Beetles ◽  
Flea Beetles

AbstractPhylogenies of representative genera based on 3 molecular markers are presented. These data are combined with previously published morphological data (Lingafelter & Konstantinov 2000). The results of separate 28S-D2 rRNA, combined analysis of molecular data and molecular data combined with morphological characters for 27 genera indicate monophyly of Galerucinae s.s. and paraphyly of Flea Beetles. These results also mean that our molecular data do not support the Flea Beetles as a tribal ranking (Alticini) within the Galerucinae, nor is it's status as a separate subfamily (Alticinae) as has been proposed by other researchers. We suggest that addition of more independent characters is still needed to resolve the question of the relationships between the Flea Beetles and the galerucines.

Genes, morphology and agreement: congruence in Australian anthracine bee flies (Diptera : Bombyliidae : Anthracinae)

2003 ◽  
Vol 17 (2) ◽  
pp. 161 ◽  
Author(s):  
Christine L. Lambkin ◽  
David K. Yeates
Keyword(s):  
Mitochondrial Dna ◽  
Morphological Analysis ◽  
Morphological Characters ◽  
Molecular Data ◽  
Simultaneous Analysis ◽  
Data Sources ◽  
Morphological Data ◽  
Base Pairs ◽  
The World ◽  
Species Groups

Almost half of the 4547 described bee flies (Bombyliidae : Diptera) in the world belong to the subfamily Anthracinae, with most of the world's diversity in three cosmopolitan tribes: Villini, Anthracini and Exoprosopini. Molecular data from 815 base pairs of 16S mitochondrial DNA and morphological characters from species-groups of these tribes in Australia were analysed cladistically. The results show that the relationships between the anthracine tribes reflect those found in a previous morphological analysis. The genera of the Anthracinae in Australia are monophyletic, except for Ligyra Newman, and are assigned to tribes. Although simultaneous analysis of the combined molecular and morphological data produced clades found in both separate analyses, the different data sources are significantly incongruent. We use phylogenetic measures to examine support for the relationships among the Australian Anthracinae inferred by the molecular and morphological data.

scholarly journals Disentangling relationships among eastern Mediterranean Cymbalaria including description of a novel species from the southern Peloponnese (Greece)

2021 ◽  
Vol 307 (2) ◽  
Author(s):  
Pau Carnicero ◽  
Núria Garcia-Jacas ◽  
Llorenç Sáez ◽  
Theophanis Constantinidis ◽  
Mercè Galbany-Casals
Keyword(s):  
Eastern Mediterranean ◽  
Phylogenetic Analyses ◽  
Taxonomic Revision ◽  
Distribution Patterns ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Long Distance ◽  
Aegean Islands ◽  
Molecular Phylogenetic

AbstractThe eastern Mediterranean basin hosts a remarkably high plant diversity. Historical connections between currently isolated areas across the Aegean region and long-distance dispersal events have been invoked to explain current distribution patterns of species. According to most recent treatments, at least two Cymbalaria species occur in this area, Cymbalaria microcalyx and C. longipes. The former comprises several intraspecific taxa, treated at different ranks by different authors based on morphological data, evidencing the need of a taxonomic revision. Additionally, some populations of C. microcalyx show exclusive morphological characters that do not match any described taxon. Here, we aim to shed light on the systematics of eastern Mediterranean Cymbalaria and to propose a classification informed by various sources of evidence. We performed molecular phylogenetic analyses using ITS, 3’ETS, ndhF and rpl32-trnL sequences and estimated the ploidy level of some taxa performing relative genome size measures. Molecular data combined with morphology support the division of traditionally delimited C. microcalyx into C. acutiloba, C. microcalyx and C. minor, corresponding to well-delimited nrDNA lineages. Furthermore, we propose to combine C. microcalyx subsp. paradoxa at the species level. A group of specimens previously thought to belong to Cymbalaria microcalyx constitute a well-defined phylogenetic and morphological entity and are described here as a new species, Cymbalaria spetae. Cymbalaria longipes is non-monophyletic, but characterized by being glabrous and diploid, unlike other eastern species. The nrDNA data suggest at least two dispersals from the mainland to the Aegean Islands, potentially facilitated by marine regressions.

Revision of Autolytinae (Syllidae: Polychaeta)

Zootaxa ◽  
2004 ◽  
Vol 680 (1) ◽  
pp. 1 ◽  
Author(s):  
ARNE NYGREN
Keyword(s):  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Sets ◽  
Nominal Species ◽  
Strict Consensus Tree ◽  
Incertae Sedis ◽  
Nomina Dubia ◽  
Morphological And Molecular Data ◽  
Combined Data

Autolytinae is revised based on available types, and newly collected specimens. Out of 170 nominal species, 18 are considered as incertae sedis, 43 are regarded as junior synonyms, and 25 are referred to as nomina dubia. The relationships of Autolytinae is assessed from 51 morphological characters and 211 states for 76 ingroup-taxa, and 460 molecular characters from mitochondrial 16S rDNA and nuclear 18S rDNA for 31 ingroup-taxa; outgroups include 12 non-autolytine syllid polychaetes. Two analyses are provided, one including morphological data only, and one with combined morphological and molecular data sets. The resulting strict consensus tree from the combined data is chosen for a reclassification. Three main clades are identified: Procerini trib. n., Autolytini Grube, 1850, and Epigamia gen. n. Proceraea Ehlers, 1864 and Myrianida Milne Edwards, 1845 are referred to as nomen protectum, while Scolopendra Slabber, 1781, Podonereis Blainville, 1818, Amytis Savigny, 1822, Polynice Savigny, 1822, and Nereisyllis Blainville, 1828 are considered

Combined molecular and morphological phylogeny of Ephemerellinae (Ephemerellidae: Ephemeroptera), with remarks about classification

Zootaxa ◽  
2009 ◽  
Vol 1991 (1) ◽  
pp. 28-42 ◽  
Author(s):  
T. HEATH OGDEN ◽  
JONATHAN T. OSBORNE ◽  
LUKE M. JACOBUS ◽  
MICHAEL F. WHITING
Keyword(s):  
Morphological Analysis ◽  
Sequence Data ◽  
Histone H3 ◽  
Morphological Characters ◽  
Morphological Data ◽  
Rdna Genes ◽  
Dna Sequence Data ◽  
Extant Genus ◽  
Species Groups ◽  
Combined Data

This study represents the first combined molecular and morphological analysis for the mayfly family Ephemerellidae (Ephemeroptera), with a focus on the relationships of genera and species groups of the subfamily Ephemerellinae. The phylogeny was constructed based on DNA sequence data from 3 nuclear (18S rDNA, 28S rDNA, histone H3) and 2 mitochondrial (12S rDNA, 16S rDNA) genes, and 23 morphological characters. Taxon sampling for Ephemerellidae included exemplars from all 25 extant genus groups and additional representatives from those genera with the highest diversity. Ephemerellidae appears to consist of three major clades. Ephemerella, the largest genus of Ephemerellidae, and Serratella were not supported as monophyletic, and each had representatives in two of the three major clades. However, the genera Drunella and Cincticostella were supported as monophyletic. Lineages strongly supported as monophyletic include a grouping of the Timpanoginae genera Timpanoga, Dannella, Dentatella and Eurylophella, and groupings of the Ephemerellinae genera Torleya, Hyrtanella and Crinitella and the genera Kangella, Uracanthella and Teloganopsis. The placement of the Timpanoginae genus Attenella fell within Ephemerellinae, based on molecular and combined data, but it grouped with other Timpanoginae based on morphological data alone. Further study and analysis of Ephemerellidae morphology is needed, and classification should be revised, if it is to reflect phylogenetic relationships.

A revision of Australian species of Radula subg. Odontoradula

2013 ◽  
Vol 26 (6) ◽  
pp. 408 ◽  
Author(s):  
Matt A. M. Renner ◽  
Nicolas Devos ◽  
Elizabeth A. Brown ◽  
Matt J. von Konrat
Keyword(s):  
New Zealand ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
South Eastern ◽  
Chloroplast Markers ◽  
Molecular Divergence ◽  
Wet Tropics ◽  
Australian Flora ◽  
Morphological Differences

The current paper presents molecular data from three chloroplast markers (atpB–rbcL spacer, trnG G2 intron, trnL–trnF intron and spacer); morphological data, and geographic data to support the recognition of nine species belonging to Radula subg. Odontoradula in Australasia. R. ocellata, the subgeneric type from the Wet Tropics bioregion, is maintained as distinct from its sister species, R. pulchella, from south-eastern Australian rainforests; both species are Australian endemics. Reinstatement of R. allisonii from synonymy, under R. retroflexa, is supported by molecular data and morphological characters, including the absence of triradiate trigones on leaf-lobe cell walls, the apex of lobules on primary shoots not being turned outwards, the oblong-elliptic female bracts, and the perianths having a pronounced wing. Reinstatement of R. weymouthiana, from synonymy under R. retroflexa, is also supported by molecular data and morphological characters, including the presence of a single low dome-shaped papilla over each leaf-lobe cell, and the large imbricate lobules on primary shoots. R. weymouthiana occurs in Tasmania and New Zealand, whereas R. allisonii is a New Zealand endemic. Australian R. retroflexa exhibits differentiation into epiphytic and rheophytic morphs, interpreted as ecotypes. Australian individuals, comprising both epiphytic and rheophytic morphs, are monophyletic and nested within a clade containing individuals from other regions. R. novae-hollandiae is newly reported for the New Zealand Botanical Region, from Raoul Island in the Kermadecs. R. novae-hollandiae exhibits decoupling of morphological and molecular divergence, with Australian individuals forming two clades reflecting geography (a Wet Tropics bioregion clade and a south-eastern Rainforest clade). These clades exhibit equivalent levels of molecular divergence, as observed in R. pulchella and R. ocellata, but no morphological differences. Similar levels of molecular divergence were observed in trans-Tasman populations of R. tasmanica. The New Zealand endemic, R. plicata, is excluded from the Australian flora, and R. cuspidata replaces R. dentifolia for the New Zealand endemic species formerly known by both names.

A proposal for a new generic structure of the killifish family Aphaniidae, with the description of Aphaniops teimorii (Teleostei: Cyprinodontiformes)

Zootaxa ◽  
2020 ◽  
Vol 4810 (3) ◽  
pp. 421-451 ◽  
Author(s):  
JÖRG FREYHOF ◽  
BARAN YOĞURTÇUOĞLU
Keyword(s):  
Original Description ◽  
Morphological Characters ◽  
Molecular Data ◽  
Zoological Nomenclature ◽  
Meristic Characters ◽  
Generic Structure ◽  
Incertae Sedis ◽  
Species Groups ◽  
Colour Patterns ◽  
Fossil Genus

Following an exhaustive review of published molecular data and morphological characters, we propose a new generic classification for the Cyprinodontiform family Aphaniidae. The generic concept applied is based on monophyly, reasonable compactness, and morphological diagnoses. The proposed genera are monophyletic and compact groups that can be diagnosed by a combination of morphological characters. All species in Aphaniidae are morphologically homogeneous and have similar scale patterns, fin positions, and meristic characters. However, only a handful of morphological characters, including colour patterns, permit identification. We propose separation of Aphaniidae into eight monophyletic genera: Anatolichthys, Aphaniops, Aphanius, Kosswigichthys, Paraphanius, and Tellia, in addition to the new genera Esmaeilius and Apricaphanius, which are described for the species of the A. sophiae and A. iberus species groups, respectively. The original description of Aphanius hormuzensis does not fulfil the criteria of the International Commission on Zoological Nomenclature, therefore this taxon is re-described as A. teimorii. Esmaeilius arakensis, E. kavirensis, E. mesopotamicus, and E. pluristriatus are treated as synonyms of E. sophiae. Esmaeilius farsicus is a junior synonym of E. persicus (Jenkins, 1910). The fossil genus Brachylebias is considered as incertae sedis since it lacks diagnostic characters which would permit it to be assigned to any of the extant genera recognised in this study. 

scholarly journals DNA Barcoding: Mixed results for big-headed flies (Diptera: Pipunculidae)

Zootaxa ◽  
2007 ◽  
Vol 1423 (1) ◽  
pp. 1-26 ◽  
Author(s):  
JEFFREY H. SKEVINGTON ◽  
CHRISTIAN KEHLMAIER ◽  
GUNILLA STÅHLS
Keyword(s):  
Dna Barcoding ◽  
Sequence Data ◽  
Phylogenetic Signal ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Base Pairs ◽  
Data Set ◽  
Taxonomic Decision ◽  
Morphological Species

Sequence data from 658 base pairs of mitochondrial cytochrome c oxidase I (cox1) were analysed for 28 described species of Pipunculidae (Diptera) in an effort to test the concept of DNA Barcoding on this family. Two recently revised but distantly related pipunculid lineages with presumed different evolutionary histories were used for the test (Clistoabdominalis Skevington, 2001 and Nephrocerus Zetterstedt, 1838). An effort was made to test the concept using sister taxa and morphologically similar sibling species swarms in these two genera. Morphological species concepts for Clistoabdominalis taxa were either supported by cox1 data or found to be too broad. Most of the discordance could be accounted for after reassessing morphological characters. In these cases, the molecular data were invaluable in assisting taxonomic decision-making. The radiation of Nearctic species of Nephrocerus could not be diagnosed using cox1. The ability of cox1 to recover phylogenetic signal was also tested on Clistoabdominalis. Morphological data for Clistoabdominalis were combined with the molecular data set. The pipunculid phylogeny from molecular data closely resembles the published phylogeny based on morphology. Partitioned Bremer support is used to localize areas of conflict between the datasets.

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