Prolactin modulates luteal regression from the coeliac ganglion via the superior ovarian nerve in the late-pregnant rat

2016 ◽  
Vol 28 (5) ◽  
pp. 565 ◽  
Author(s):  
Sandra S. Vallcaneras ◽  
Magalí de la Vega ◽  
Silvia M. Delgado ◽  
Alicia Motta ◽  
Carlos Telleria ◽  
...  
Keyword(s):  
Pregnant Rat ◽  
Luteal Regression ◽  
Luteal Function ◽  
Prostaglandin F2 Alpha ◽  
Superior Ovarian Nerve ◽  
Polymerase Chain ◽  
Oxide Synthase ◽  
Experimental Group ◽  
Gain Access ◽  
Inducible Nitric Oxide

There is considerable evidence of the neuroendocrine control involved in luteal regression in the rat. In addition, circulating prolactin (PRL), which increases during the night before parturition, may gain access to the coeliac ganglion (CG), indirectly impacting the physiology of the ovary because of the known connection between the CG and the ovary via the superior ovarian nerve (SON). In this work we investigated in the CG–SON–ovary system and whether PRL added to the CG has an impact, indirectly via the SON, on luteal regression on Day 21 of pregnancy. The system was incubated without (control) or with PRL added to the CG. We measured the ovarian release of progesterone (P), oestradiol and prostaglandin F2 alpha (PGF2α) by radioimmunoassay, and nitrites (NO) by the Griess method. Luteal mRNA expression of 3β-hydroxysteroid dehydrogenase (3β-HSD), 20α-HSD, aromatase, inducible nitric oxide synthase (iNOS) and apoptosis regulatory factors was analysed by reverse transcription–polymerase chain reaction. P release, the expression of Bcl-2 and the Bcl-2 : Bax ratio was lower than control preparations, while the expression of 20α-HSD and the release of NO and PGF2α were higher in the experimental group. In conclusion, PRL acts at the CG and, by a neural pathway, modulates luteal function at the end of pregnancy.

scholarly journals Nitric oxide synthases in pregnant rat uterus

Reproduction ◽  
2001 ◽  
pp. 403-407 ◽  
Author(s):  
M Farina ◽  
ML Ribeiro ◽  
A Franchi
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Nitric Oxide Synthesis ◽  
Uterine Contractility ◽  
Pregnant Rat ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Nitric Oxide Synthase Activity ◽  
Inducible Nitric Oxide

The conversion of [14C]arginine into [14C]citrulline as an indicator of nitric oxide synthesis was studied in uteri isolated from rats on different days of gestation, after labour and during dioestrus. Nitric oxide synthesis was present in uterine tissues isolated at each stage of gestation and also in tissues collected during dioestrus and after labour. Expression of neuronal nitric oxide synthase was not detectable at any of the stages studied. Endothelial nitric oxide synthase was present at all the stages studied, but there was a significant increase on day 13 of gestation and a decrease thereafter, with the lowest expression recorded on the day after labour. Inducible nitric oxide synthase expression in rat uteri increased substantially during pregnancy, with the highest expression on day 13 of gestation; expression decreased at term and after labour. The changes in expression of inducible nitric oxide synthase were coincident with the changes in nitric oxide synthase activity in uteri treated with aminoguanidine. Thus, these findings indicate that an increase in expression of inducible nitric oxide synthase in the uterus may be important for maintenance of uterine quiescence during pregnancy and its decrease near the time of labour could have an effect on the start of uterine contractility.

Localization of Inducible Nitric Oxide Synthase mRNA in Inflamed Gastrointestinal Mucosa byin SituReverse Transcriptase–Polymerase Chain Reaction

Nitric Oxide ◽  
1998 ◽  
Vol 2 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Xiao-Jing Zhang ◽  
Jane H Thompson ◽  
Elizabeth E Mannick ◽  
Pelayo Correa ◽  
Mark J.S Miller
Keyword(s):  
Nitric Oxide ◽  
Polymerase Chain Reaction ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Gastrointestinal Mucosa ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Exosome miR-155-5p Derived from Lung Cancer Hcc827 Promotes Macrophage Activation and Lung Cancer Progression

2022 ◽  
Vol 12 (1) ◽  
pp. 199-205
Author(s):  
Yanxin Ma ◽  
Dongmei Wang ◽  
Songzhi Luo ◽  
Zhiwei He ◽  
Jiannan Sun
Keyword(s):  
Lung Cancer ◽  
Cancer Progression ◽  
Cell Invasion ◽  
Control Group ◽  
Lung Cancer Cell ◽  
Matrix Metallopeptidase ◽  
Matrix Metallopeptidase 9 ◽  
Oxide Synthase ◽  
Experimental Group ◽  
Inducible Nitric Oxide

This stud intends to assess whether exosome miR-155-5p derived from human non-small cell lung cancer cells (Hcc827) activates macrophages in lung cancer. Lung cancer Hcc827 cells were assigned into control group and expeirmental group (cultured with macrophages, THP-1 activated by exosome miR-155-5P derived from Hcc827) followed by analysis of macrophage markers inducible nitric oxide synthase (INOS), recombinant human CD163 (CD163), matrix metallopeptidase 9 (MMP9), matrix metallopeptidase 2 (MMP2), and E-cadherin by real-time fluorescent quantitative PCR (RFQ-PCR), IL-10, IL-6 and IL-8 levels by chemiluminescence, cell invasion by Transwell assay and related protein expression by Western blot. miR-155-5p treatment significantly reduced INOS and TNF-β expressions and increased CD163, TNF-α, IL-8, IL-6 and IL-10 expressions along with enhanced cell invasion. In addition, MMP9 and MMP2 expressions in experimental group were significantly increased and E-cdherin was reduced. In conclusion, exosome miR-155-5p derived from lung cancer Hcc827 cells activates macrophages and enhanced lung cancer cell invasion.

scholarly journals Regulation of Inducible Nitric Oxide Synthase Messenger Ribonucleic Acid Expression in Pregnant Rat Uterus1

1998 ◽  
Vol 59 (4) ◽  
pp. 933-940 ◽  
Author(s):  
Y.-L. Dong ◽  
L. Fang ◽  
P.R.R. Gangula ◽  
C. Yallampalli
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Ribonucleic Acid ◽  
Pregnant Rat ◽  
Messenger Ribonucleic Acid ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Cerebellar stimulation reduces inducible nitric oxide synthase expression and protects brain from ischemia

1998 ◽  
Vol 274 (6) ◽  
pp. H2035-H2045 ◽  
Author(s):  
Elena Galea ◽  
Eugene V. Golanov ◽  
Douglas L. Feinstein ◽  
Keith A. Kobylarz ◽  
Sara B. Glickstein ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Inos Mrna ◽  
Cerebral Microvessels ◽  
Spontaneously Hypertensive ◽  
Polymerase Chain ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide ◽  
Stimulation Of

A focal infarction produced by occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rats induced expression of inducible nitric oxide synthase (iNOS) mRNA, measured by competitive reverse transcription-polymerase chain reaction. The mRNA appeared simultaneously in the ischemic core and penumbra at 8 h, peaked between 14 and 24 h, and disappeared by 48 h. At 24 h, inducible nitric oxide synthase (iNOS)-like immunoreactivity was present in the endothelium of cerebral microvessels and in scattered cells, probably representing leukocytes or activated microglia. Electrical stimulation of the cerebellar fastigial nucleus (FN) for 1 h, 48 h before MCAO, reduced infarct volumes by 45% by decreasing cellular death in the ischemic penumbra. It also reduced by >90% the expression of iNOS mRNA and protein in the penumbra, but not core, and decreased by 44% the iNOS enzyme activity. We conclude that excitation of neuronal networks represented in the cerebellum elicits a conditioned central neurogenic neuroprotection associated with the downregulation of iNOS mRNA and protein. This neuroimmune interaction may, by blocking the expression of iNOS, contribute to neuroprotection.

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