Successful sperm cryopreservation in Egyptian spiny mice Acomys cahirinus

The menstruating Egyptian spiny mouse has recently been proposed as a new animal model for reproductive health research. Unfortunately, little is known about reproduction in males. This study compared several characteristics of sperm function before and after cryopreservation. Epididymal spermatozoa were cryopreserved in different concentrations of raffinose and skim milk and tested for motility and membrane integrity (Experiment 1). Further evaluations of motility, plasma membrane and acrosome integrity, mitochondrial membrane potential and DNA integrity were conducted with the addition of l-glutamine to the extender (Experiment 2). The results show that, following cryopreservation, motility and membrane integrity were reduced, but were better maintained in the presence of l-glutamine (P<0.05). Moreover, although all sperm parameters were significantly reduced following cryopreservation (P<0.05), most cryopreserved spermatozoa retained acrosome, membrane and DNA integrity while also maintaining motility and mitochondrial membrane potential. This study provides a new step towards the development of assisted reproductive techniques and archiving the important genetics of the world’s only known menstruating rodent.

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Effects of DHA-rich fish oil supplementation on the lipid profile, markers of muscle damage, and neutrophil function in wheelchair basketball athletes before and after acute exercise

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2014-0140 ◽

2015 ◽

Vol 40 (6) ◽

pp. 596-604 ◽

Cited By ~ 13

Author(s):

Camila Garcia Marques ◽

Vinicius Coneglian Santos ◽

Adriana Cristina Levada-Pires ◽

Thiago Manzoni Jacintho ◽

Renata Gorjão ◽

...

Keyword(s):

Membrane Potential ◽

Lipid Profile ◽

Muscle Damage ◽

Mitochondrial Membrane Potential ◽

Mitochondrial Membrane ◽

Acute Exercise ◽

Membrane Integrity ◽

Neutrophil Function ◽

Phagocytic Capacity ◽

Before And After

We investigated the effects of docosahexaenoic acid (DHA)-rich fish oil (FO) supplementation on the lipid profile, levels of plasma inflammatory mediators, markers of muscle damage, and neutrophil function in wheelchair basketball players before and after acute exercise. We evaluated 8 male basketball wheelchair athletes before and after acute exercise both prior to (S0) and following (S1) FO supplementation. The subjects were supplemented with 3 g of FO daily for 30 days. The following components were measured: the plasma lipid profile (total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides), plasma inflammatory mediators (C-reactive protein, interleukin (IL)-1β, IL-1ra, IL-4, IL-6, IL-8, and tumor necrosis factor-α), markers of muscle damage (creatine kinase and lactate dehydrogenase (LDH)), and neutrophil function (cytokine production, phagocytic capacity, loss of membrane integrity, mitochondrial membrane potential, neutral lipid accumulation, phosphatidylserine externalization, DNA fragmentation, and production of reactive oxygen species (ROS)). Acute exercise increased the plasma levels of total cholesterol, LDH, IL1ra, and IL-6, led to the loss of membrane integrity, ROS production, and a high mitochondrial membrane potential in neutrophils, and reduced the phagocytic capacity and IL-6 production by the neutrophils (S0). However, supplementation prevented the increases in the plasma levels of LDH and IL-6, the loss of membrane integrity, and the alterations in ROS production and mitochondrial membrane potential in the neutrophils that were induced by exercise (S1). In conclusion, DHA-rich FO supplementation reduces the markers of muscle damage, inflammatory disturbances, and neutrophil death induced by acute exercise in wheelchair athletes.

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Antisperm antibodies and sperm function in bulls undergoing scrotal insulation

Reproduction ◽

10.1530/rep-20-0207 ◽

2020 ◽

Vol 160 (5) ◽

pp. 783-792

Author(s):

Maria S Ferrer ◽

Roberto Palomares ◽

David Hurley ◽

Anna-Claire Bullington ◽

Alejandro Hoyos-Jaramillo ◽

...

Keyword(s):

Lipid Peroxidation ◽

Membrane Potential ◽

Mitochondrial Membrane Potential ◽

Mitochondrial Membrane ◽

Membrane Integrity ◽

Sperm Function ◽

Antisperm Antibodies ◽

Scrotal Circumference ◽

Acrosome Integrity ◽

Scrotal Insulation

Bovine antisperm antibodies (ASAs) have been associated with teratospermia and asthenospermia. It was hypothesized here that scrotal insulation induces the formation of ASAs and deterioration of sperm function. Scrotal insulation bags were placed in 10 bulls for 8 days. Semen was collected on days −29, −22 and −2, twice weekly from days 5 to 54, and thereafter weekly until day 96 (day 0 = first day of scrotal insulation). On each collection day, scrotal circumference, sperm motility, morphology, membrane integrity, acrosome integrity, apoptosis, lipid peroxidation, mitochondrial membrane potential, ASA binding and DNA integrity were evaluated. The percentage of IgG- and IgA-bound sperm increased between days 12 and 96 (P < 0.0001), in association with poor motility (days 19–30, P < 0.005) and morphology (days 8–40, P < 0.0001). Mean scrotal circumference decreased between days 15 and 75 (P < 0.0001). There was also a deterioration in sperm membrane integrity (days 19–40, P < 0.0001), acrosome integrity (days 26–89, P < 0.0001), lipid peroxidation (days 5–12, P < 0.0001), and mitochondrial membrane potential (days 12–96, P = 0.001). In contrast, a decrease in apoptotic cells (days 37–83, P = 0.0002) and lipid peroxidation (days 19–96, P < 0.0001) was noticed. Most bulls recovered normospermia by day 96. However, the persistence of ASAs, acrosomal damage and dysfunctional mitochondria suggest a long term effect of scrotal insulation on sperm function and the homeostasis of the reproductive immune system.

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Effect of diode laser on motility, plasma and acrosomal membrane integrity, and mitochondrial membrane potential of cryopreserved stallion spermatozoa

Animal Reproduction Science ◽

10.1016/j.anireprosci.2008.05.086 ◽

2008 ◽

Vol 107 (3-4) ◽

pp. 309-310

Author(s):

A.C. Brandão ◽

R.P. Arruda ◽

A.F.C. Andrade ◽

F.G. Zaffalon ◽

O.F.B. Tarragó ◽

...

Keyword(s):

Membrane Potential ◽

Diode Laser ◽

Mitochondrial Membrane Potential ◽

Mitochondrial Membrane ◽

Membrane Integrity ◽

Acrosomal Membrane

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Evaluation of epididymis storage temperature and cryopreservation conditions for improved mitochondrial membrane potential, membrane integrity, sperm motility and in vitro fertilization in bovine epididymal sperm

Reproduction in Domestic Animals ◽

10.1111/rda.12888 ◽

2016 ◽

Vol 52 (2) ◽

pp. 257-263 ◽

Cited By ~ 16

Author(s):

M Nichi ◽

T Rijsselaere ◽

JDA Losano ◽

DSR Angrimani ◽

GKV Kawai ◽

...

Keyword(s):

Membrane Potential ◽

In Vitro Fertilization ◽

Mitochondrial Membrane Potential ◽

Sperm Motility ◽

Mitochondrial Membrane ◽

Storage Temperature ◽

Membrane Integrity ◽

Epididymal Sperm ◽

Vitro Fertilization

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Seminal plasma has limited counteracting effects following induction of oxidative stress in donkey spermatozoa

Reproduction Fertility and Development ◽

10.1071/rd19192 ◽

2020 ◽

Vol 32 (6) ◽

pp. 619

Author(s):

Marion Papas ◽

Jaime Catalan ◽

Sebastián Bonilla-Correal ◽

Sabrina Gacem ◽

Jordi Miró ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Membrane Potential ◽

Mitochondrial Membrane Potential ◽

Sperm Motility ◽

Seminal Plasma ◽

Mitochondrial Membrane ◽

Skim Milk ◽

Sperm Parameters ◽

High Concentration

The aim of this study was to evaluate the response of donkey spermatozoa to oxidative stress induced by hydrogen peroxide, and to determine whether the presence of seminal plasma modulates the sperm response to that stress. Nine ejaculates were collected, extended in skim milk extender and split into two aliquots. Seminal plasma was removed from the first but not second aliquot. Samples were subsequently split into four aliquots supplemented with different concentrations of commercial hydrogen peroxide (0, 100 and 250µM and 50mM). Aliquots were incubated at 37°C under aerobic conditions and several sperm parameters, namely motility, viability, intracellular levels of peroxides and superoxides and mitochondrial membrane potential, were evaluated at 0, 1 and 3h. Exposure to hydrogen peroxide markedly decreased sperm motility but had much less of an effect on sperm viability, mitochondrial membrane potential and intracellular reactive oxygen species levels. A protective effect of seminal plasma against the loss of sperm motility was not apparent, but some kinetic parameters and relative levels of superoxides were better maintained when seminal plasma was present together with high concentration of hydrogen peroxide. In conclusion, oxidative stress induced by hydrogen peroxide reduces donkey sperm motility and has a less apparent effect on other sperm parameters. Finally, seminal plasma is only able to partially ameliorate the detrimental effect of this induced stress.

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