Superovulation of Merino ewes with an ovine pituitary follicle stimulating hormone extract

1991 ◽  
Vol 3 (5) ◽  
pp. 561 ◽  
Author(s):  
HN Jabbour ◽  
G Evans
Keyword(s):  
Luteinizing Hormone ◽  
Field Trial ◽  
Follicle Stimulating Hormone ◽  
Mouse Bioassay ◽  
Protein Fractionation ◽  
Potency Ratio ◽  
Fertilization Rates ◽  
The Mean ◽  
Fresh Semen ◽  
Stimulating Hormone

In order to investigate the effectiveness of ovine FSH as a superovulatory gonadotrophin in sheep, three batches (oFSH-1, oFSH-2 and oFSH-3) of crude follicle stimulating hormone (FSH) extracts were prepared from ovine pituitaries by protein fractionation. The extracts were characterized in radioimmunoassays (RIA), radioreceptor assays (RRA) and bioassays. A FSH:LH (luteinizing hormone) ratio of 1:0.71 and 1:0.18 was calculated from oFSH-1 in RIA and RRA respectively. oFSH-1 was more potent than NIH-oFSH-S12 in RRA, with a potency ratio of 1:0.65 calculated at 50% binding. In mouse bioassay, oFSH-2 was effective in inducing folliculogenesis. The potency of oFSH-3 as an ovarian stimulatory hormone was tested in a field trial on sheep. Thirty-one mature Merino ewes were treated in the breeding season with intravaginal progestagen pessaries and 6, 12 or 24 mg doses of oFSH-3 were injected twice daily for 3 days (n = 10 or 11). Each ewe was inseminated in the uterus with fresh semen 24 h after pessary withdrawal. Ova or embryos were recovered by mid-ventral laparotomy 6 or 7 days after pessary withdrawal. The dose of oFSH-3 had a significant effect on the ovulatory response (P less than 0.01); the mean ovulation rates were 1.5 +/- 0.2, 12.6 +/- 2.8 and 13.1 +/- 2.9 following injection with 6, 12 or 24 mg of the gonadotrophin respectively. The dose of the gonadotrophin injected had no effect on the mean percentages of ova recovered or fertilized. The overall ova recovery and fertilization rates (%) were 67.8 +/- 6.4 and 92.2 +/- 5.1 respectively. (ABSTRACT TRUNCATED AT 250 WORDS)

SERUM FOLLICLE-STIMULATING HORMONE, LUTEINIZING HORMONE AND PROGESTERONE CONCENTRATIONS IN PSEUDOPREGNANT RATS TREATED WITH MEDROXYPROGESTERONE ACETATE

1976 ◽  
Vol 70 (3) ◽  
pp. 465-471 ◽  
Author(s):  
W. J. DE GREEF ◽  
J. DULLAART ◽  
G. H. ZEILMAKER
Keyword(s):  
Luteinizing Hormone ◽  
Medroxyprogesterone Acetate ◽  
Follicle Stimulating Hormone ◽  
Corpora Lutea ◽  
Progesterone Concentration ◽  
Vaginal Smears ◽  
The Mean ◽  
And Control ◽  
Stimulating Hormone

SUMMARY Pseudopregnant rats were treated early in pseudopregnancy with 1 or 10 mg medroxyprogesterone acetate (MPA). Serum FSH, LH and progesterone concentrations were determined on days 2–20 of pseudopregnancy in treated and control rats. The mean duration of pseudopregnancy was 13·5 days in the control animals, but when animals were treated with 1 mg MPA a dioestrous period of 21·4 days was observed. A period with leucocytic vaginal smears of at least 2 months was observed after treatment with 10 mg MPA. Injection with MPA on day 3 of pseudopregnancy did not affect the serum FSH concentrations during the subsequent days. The progesterone pattern was alike in the three groups of animals, i.e. the duration of the activity of the corpora lutea was similar in all groups. However, 10 mg MPA slightly lowered progesterone concentrations on days 4–8 of pseudopregnancy. In the saline-treated rats, LH concentrations decreased from days 2–5, and remained low until they increased after day 11 of pseudopregnancy. This increase was delayed until day 20 in the animals treated with 1 mg MPA, and was not observed in the animals treated with 10 mg MPA. It is argued that the increase of LH concentration at the end of pseudopregnancy is not instrumental in the decrease of peripheral progesterone concentration but rather that the decrease in the progesterone concentration leads to the increase in the LH concentration.

URINARY GONADOTROPHINS FOLLOWING CASTRATION OF MEN

1973 ◽  
Vol 74 (1) ◽  
pp. 79-87
Author(s):  
Peter Christiansen
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Maximal Level ◽  
The Mean ◽  
Normal Men ◽  
Stimulating Hormone

ABSTRACT The excretion of total hypophyseal gonadotrophins (HG), follicle stimulating hormone (FSH) and luteinizing hormone (LH) was studied in 13 castrated men aged 23 to 56 years, mean 38.8 years. All the men were castrated for legal reasons. The time from castration to the investigation varied from 4 weeks to 24 years. Four of the subjects were studied from the time before surgery until 4 weeks after castration. The mean excretion of HG, FSH and LH was statistically significantly higher than that of normal men of the same age i. e. by 8, 9 and 3 times higher respectively. The FSH/LH ratio was in the mean 2.8. Following orchiectomy FSH rose more rapidly and to a higher level than LH. The maximal level of FSH was reached within 1–2 weeks after castration in contrast to LH which did not consistently reach the maximal level within 4 weeks. This difference in FSH and LH levels after castration supports the concept that FSH and LH are regulated independently in man.

URINARY FOLLICLE STIMULATING HORMONE AND LUTEINIZING HORMONE IN SIX MALES WITH THE XYY SYNDROME

1973 ◽  
Vol 74 (4) ◽  
pp. 625-630 ◽  
Author(s):  
Peter Christiansen ◽  
Johannes Nielsen
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Normal Range ◽  
Xyy Syndrome ◽  
The Mean ◽  
Normal Males ◽  
Stimulating Hormone

ABSTRACT Urinary follicle stimulating hormone (FSH) and luteinizing hormone (LH) were studied in 6 males with karyotype 47, XYY and compared to FSH and LH in 14 normal males of the same age. FSH was within the normal range in all 6 subjects, LH however was markedly elevated in one case. The FSH/LH ratio was thus decreased in one case but normal in the remaining 5 cases. The mean excretion of FSH and LH did not differ significantly from that of normal males.

Inhibition of luteinizing hormone, follicle-stimulating hormone and sex-steroid levels in men and women with a potent antagonist analog of luteinizing hormone-releasing hormone, Cetrorelix (SB-75)

1994 ◽  
Vol 131 (3) ◽  
pp. 286-292 ◽  
Author(s):  
David Gonzalez-Barcena ◽  
Manuel Vadillo Buenfil ◽  
Emilo Garcia Procel ◽  
Laura Guerra-Arguero ◽  
Imelda Cardenas Cornejo ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Free Testosterone ◽  
Serum Levels ◽  
Routes Of Administration ◽  
Serum Lh ◽  
The Mean ◽  
Potent Antagonist ◽  
Normal Men ◽  
Stimulating Hormone

Gonzalez-Barcena D, Vadillo Buenfil M, Garcia Procel E, Guerra-Arguero L, Cardenas Cornejo I, Comaru-Schally AM, Schally AV. Inhibition of luteinizing hormone, follicle-stimulating hormone and sex-steroid levels in men and women with a potent antagonist analog of LH-RH, Cetrorelix (SB-75). Eur J Endocrinol 1994;131:286–92. ISSN 0804–4643 Cetrorelix (SB-75; [Ac-d-Nal(2)1, d-Phe(4Cl)2, d-Pal(3)3, d-Cit6, d-Ala10] luteinizing hormone-releasing hormone (LHRH)) is a new highly potent antagonist analog of LHRH containing the d-ureidoalkyl amino acid d-citrulline at position 6 and is free of allergenic effects. This study shows the inhibition of LH and follicle-stimulating hormone (FSH) release in normal men, postmenopausal women and patients with gonadal dysgenesis, using different doses and im, sc and iv routes of administration of SB-75. The mean serum levels of LH and FSH in normal men who received one single dose of 300 μg of SB-75 sc started to decline rapidly 1 h after its administration; the LH suppression was sustained for 14 h and that of FSH up to 24 h or longer as the samples were obtained only up to this time. The nadir for LH was reached at 14 h and that for FSH at 24 h or later after administration of the antagonist (p < 0.05). Serum levels of total and free testosterone decreased after the first hour and this inhibition was maintained for up to 14 h. The nadir for total testosterone was at 6 h and that for free testosterone was at 8 h (p < 0.001), corresponding to 56% and 60% of inhibition, respectively. In postmenopausal women, inhibition of the elevated basal serum LH and FSH levels occurred after a single injection of the antagonist analog SB-75 in doses of 75, 150, 300, 600 and 1200 μg using im, sc and iv routes of administration. The mean resting levels of serum LH and FSH showed a significant decrease for all doses and routes of administration of SB-75 (p < 0.01). Maximal inhibition was observed 6–12 h after administration. After administration of 300 μg of SB-75 sc every 12 h for 3 days, serum LH and FSH continued to be secreted but a marked decrease in the basal levels of both gonadotropins was observed. A fall in LH and FSH also was produced in patients with gonadal dysgenesis who were given 300 μg of SB-75. The nadir of serum LH was 61 ± 9.6% for the iv route and 58.5 ± 7.5% for the sc route (p < 0.01); for serum FSH it was 51 ± 7.5% and 48.5 ± 7.5% (p < 0.01), respectively, of the baseline levels. These results show that the antagonistic analog SB-75 is devoid of allergenic effects, extremely active in small doses and can be administered safely to humans. The development of sustained delivery systems for SB-75 should facilitate the clinical use of this powerful LHRH antagonist. David Gonzalez-Barcena, Hospital de Especialidades Centro Medico La Raza, Seris Y Zaachila, Col. La Raza, Mexico D.F.

Influence of nutrition on pre-partum plasma levels of progesterone and total oestrogens and post-partum plasma levels of luteinizing hormone and follicle stimulating hormone in suckling cows

1983 ◽  
Vol 37 (1) ◽  
pp. 89-96 ◽  
Author(s):  
D. Gauthier ◽  
M. Terqui ◽  
P. Mauleon
Keyword(s):  
Luteinizing Hormone ◽  
Plasma Levels ◽  
Post Partum ◽  
Control Diet ◽  
Follicle Stimulating Hormone ◽  
Control Group ◽  
Luteinizing Hormone Concentration ◽  
The Mean ◽  
Daily Gain ◽  
Stimulating Hormone

ABSTRACTTwo groups of suckling Charolais cows were fed at different levels of nutrition from 70 days before the estimated date of calving until 45 days after calving, to study the effects of reduced dietary intake on pre-partum plasma levels of progesterone and total oestrogens (conjugated + unconjugated) and post-partum plasma levels of luteinizing hormone and follicle stimulating hormone. Eight control cows received a ration containing the Institut National de la Recherche Agronomique's recommendations for all nutrients, while 10 cows received a restricted diet which supplied approximately three-quarters of the recommended amounts for energy and nitrogen.The mean weight of the two groups of cows was similar at the beginning of the experiment but the restricted group had a significantly lower weight just before calving than the control group (678 v. 766 kg; P < 0·05).After calving, cows ont he low diet had a weight loss of 600 g/day, while those on the control diet gained 300 g/day. During the last 45 days of pregnancy underfeeding did not significantly affect oestrogen levels, but progesterone levels increased proportionally by 0·24 (P < 0·01).During the first 45 days of lactation underfeeding decreased the number of cows showing oestrus (0/10 v. 4/8) and significantly decreased both luteinizing hormone and follicle stimulating hormone levels at 5, 15 and 30 days post partum.Furthermore, the correlation between mean daily gain (0 to 45 days post partum) and luteinizing hormone concentration on day 30 was highly significant (r = 0·58; P < 0·01; no. = 19). These results suggest that the delay of the first post-partum ovulation in the underfed cow is due to low gonadotropin levels.

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