226 INCLUSION OF BOVINE SOMATOROPIN ON MULTIPLE-OVULATION EMBRYO TRANSFER TREATMENTS FOR LOW-RESPONDING BEEF DONOR COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 225
Author(s):  
S. Kmaid ◽  
J. M. Saldaña ◽  
Z. Ramos ◽  
R. Ungerfeld
Keyword(s):  
Animal Health ◽  
Control Treatment ◽  
Embryo Production ◽  
Multiple Ovulation ◽  
Oestradiol Benzoate ◽  
Pfizer Animal Health ◽  
Universal Laboratory ◽  
To Receive ◽  
Test Treatment ◽  
Donor Cows

An experiment was designed to determine if embryo production of Angus donors could be improved by including a single dose of bovine somatotropin (bST) before starting the superovulatory treatment (SPO). Thirty-three multiparous Angus cows (body condition = 4.6 ± 0.6, range = 1–8) were submitted to 2 SPO treatments at random stages of the oestrous cycle in a crossover design (60 days apart). On Day 0, cows were divided at random to receive either 500 mg of bST (n = 18; Lactotropin, Elanco Saude Animal, Brasil) or remain without bST (control, n = 15). At the same time, all cows received 2 mg of oestradiol benzoate, 50 mg of progesterone and a CIDR (Pfizer Animal Health, Uruguay). On Day 2, all cows received 200 IU of eCG (Inducel, Universal Laboratory, Uruguay) and on Day 4 were superstimulated with 425 IU of FSH (Pluset, Calier, Spain) in twice-daily decreasing doses over 4 days. On Day 6, all cows received 2 doses (a.m. and p.m.) of a PGF analogue (800 μg, Delprostenate, Glandinex, Universal Laboratory, Uruguay) and the CIDRs were removed in the morning of Day 7. Cows were injected with 10 μg of GnRH (Receptal, Intervet, the Netherlands) on Day 8 (a.m.) and were inseminated 12, 24 and 36 h later. On Day 15, ova/embryos were collected nonsurgically and evaluated in accordance to IETS guidelines. For statistical analysis, cows were categorized according to the number of transferable embryos obtained in control treatment (without bST) in terciles: low-responding cows (LR, ≤6; n = 11), middle-responding cows (7–9, n = 12), or high-responding cows (HR ≥ 10; n = 10). Data were analysed by a paired t-test. Treatment with bST increased the total ova and embryos and the number of transferable embryos in LR cows (Table 1). However, the number of transferable embryos and the percentage of transferable embryos werereduced in HR cows. The inclusion of a single bST dose 4 days before initiating SPO with FSH treatments may be a useful alternative to improve embryo production in low-responding cows. Table 1.Response of low-responding and high-responding Angus cows to superovulatory treatments with or without bST (mean ± SD)

320 INFLUENCE OF PROGESTERONE AND MOMENT OF ESTRADIOL BENZOATE INJECTION ON MULTIPLE-OVULATION EMBRYO TRANSFER RESULTS

2013 ◽  
Vol 25 (1) ◽  
pp. 307
Author(s):  
S. Kmaid ◽  
J. M. Saldaña
Keyword(s):  
Negative Binomial ◽  
Body Condition Score ◽  
Animal Health ◽  
Prostaglandin F2α ◽  
Control Group ◽  
Negative Binomial Regression Model ◽  
Embryo Production ◽  
Oestradiol Benzoate ◽  
The Moment ◽  
Universal Laboratory

Current superovulatory treatment combines progesterone as well as oestradiol injection at the moment of CIDR insertion to improve synchronization of follicular wave emergence [reviewed by Bo et al. 2002 Theriogenology 57, 53–172; and Mapletoft et al. 2003 J. Anim. Sci. 81 (E. Suppl.), E28–E36] The aim of the present work was to study if delaying oestradiol benzoate (EB) injection 24 or 48 h from CIDR insertion would have the same outcome on embryo production after superovulatory treatment as additional progesterone administered at CIDR insertion. One hundred and eleven superovulatory treatments conducted during the same period (5 consecutive collection days) at random stages of the oestrus cycle at the same location were retrospectively evaluated. Holstein cycling heifers of 18 months of age (body condition score 3.2 ± 0.28, 1–5 range; weighing 357 ± 28 kg) were divided according to the following treatments: control group (n = 24): Day 0: CIDR insertion (Pfizer Animal Health, New York, NY, USA), 50 mg of progesterone, and 2 mg of oestradiol benzoate (Benzadiol, Universal Laboratory, Montevideo, Uruguay); Group BE24 (n = 47): Day –1: CIDR insertion and Day 0: 2 mg of oestradiol benzoate; Group BE48 (n = 40): Day –2: CIDR insertion and Day 0: 2 mg of oestradiol benzoate. All heifers were superstimulated on Day 3.5 (Day 0 = EB injection) with 290 NIH units of FSH (Folltropin, BionicheAnimal Health, Belleville, ON, Canada) in twice-daily decreasing doses over 4.5 days. On Day 6, all cows received 2 doses (a.m. and p.m.) of a prostaglandin F2α analogue (800 µg, Delprostenate, Glandinex, Universal Laboratory), and CIDR were removed on the morning of Day 7. Cows were injected with 10 µg of GnRH (Receptal, Intervet, Boxmeer, the Netherlands) on Day 8 a.m. and were inseminated 12 and 24 h later with semen of 3 bulls of proven fertility. On Day 15, ova/embryos were collected nonsurgically and evaluated in accordance with IETS guidelines. Data were analysed with a negative binomial regression model (Table 1). Administration of EB 24 or 48 h after CIDR insertion produced similar numbers of transferable and freezable embryos as well as total ova/embryos compared with addition of progesterone and EB at the moment of CIDR insertion. According to these results, EB could be administrated without progesterone injection either 24 or 48 h after CIDR insertion without compromising embryo production. Table 1.Embryo production of heifers superstimulated after additional progesterone (P4) and different times (24 or 48 h after CIDR insertion) of oestradiol benzoate administration (results are mean ± SE)

13 EFFECTS OF PLASMA PROGESTERONE CONCENTRATIONS ON LH RELEASE AND OVULATION IN BEEF CATTLE GIVEN GnRH

2006 ◽  
Vol 18 (2) ◽  
pp. 115
Author(s):  
H. Davis ◽  
M. Colazo ◽  
M. Rutledge ◽  
J. Small ◽  
J. Kastelic ◽  
...  
Keyword(s):  
Animal Health ◽  
Beef Cows ◽  
Dominant Follicle ◽  
Plasma Progesterone ◽  
Lactating Cows ◽  
Significant Difference ◽  
Prostaglandin F ◽  
Pfizer Animal Health ◽  
Lh Release ◽  
To Receive

Two experiments were conducted to determine the effects of plasma progesterone (P4) concentrations on LH release and ovulation in beef heifers and lactating beef cows given gonadotropin-releasing hormone (GnRH). Previously autoclaved, once-used CIDR inserts (Colazo et al. 2004 Anim. Reprod. Sci. 81, 25-34) were used for experimental purposes to induce differential plasma progesterone concentrations. In Experiment 1, postpubertal heifers received 25 mg of dinoprost i.m. (prostaglandin F (PGF); Lutalyse; Pfizer Animal Health, Montreal, Quebec, Canada). On Day 4 (estrus = Day 0), heifers were randomly assigned (10/group) to receive no treatment (control) or 1 or 2 autoclaved once-used CIDR (Pfizer Animal Health) inserts (1CIDR and 2CIDR, respectively). On Day 5, heifers in the 1CIDR group were given PGF twice 12 h apart. On Day 6, all heifers received 100 �g of GnRH i.m. (Cystorelin; Merial Canada, Inc., Victoriaville, Quebec, Canada). Once daily on Days 4 to 9, a blood sample was collected and ultrasonography was performed. On Day 6, heifers in the control (3.0 � 0.4 ng/mL; mean � SD) and 1CIDR groups (3.0 � 0.3 ng/mL) had lower (P < 0.01) plasma progesterone concentrations than those in the 2CIDR group (5.7 � 0.4 ng/mL). However, the diameter of the dominant follicle was larger (P < 0.001) in heifers in the control and 1CIDR groups than in the 2CIDR group (12.1 � 1.0, 11.5 � 0.7, and 10.1 � 0.7 mm, respectively). More (P < 0.01) heifers ovulated in response to GnRH in the control and 1CIDR groups than in the 2CIDR group (10/10, 9/10, and 3/10, respectively). In Experiment 2, ultrasound-guided follicular ablation (FA) was performed (to synchronize ovarian follicular wave emergence) 4 to 6 days after estrus in 20 postpubertal heifers and 20 mature lactating cows. Cattle were randomly and equally assigned to receive an autoclaved, once-used CIDR, either with no further treatment (High-P4) or with two PGF treatments 12 h apart (Low-P4) given after FA. All cattle received 100 �g of GnRH either 6 days after FA or the day after the dominant follicle reached 9 mm in diameter. Ultrasonography was performed daily (from 4 days after FA to ovulation or to 3 days after GnRH treatment). In three cows and three heifers per group, blood samples were collected every 30 min for 12 h after GnRH. The dominant follicle at GnRH treatment was larger in cows than heifers (11.0 � 1.1 vs. 10.3 � 0.9 mm, respectively; P = 0.05) and tended to be smaller in the High-P4 group vs. the Low-P4 group (10.3 � 1.0 vs. 11.0 � 1.0 mm; P = 0.06). Ovulatory response was not different (P = 0.9) between heifers (77.7%) and cows (78.9%), but combined for heifers and cows, was lower in High-P4 vs. Low-P4 cattle (61.1 vs. 94.7; P < 0.01). The GnRH-induced LH surge did not differ (P = 0.23) between cows and heifers, but it was lower and of shorter duration (P < 0.001) in the High-P4 group than in the Low-P4 group. In summary, higher plasma P4 concentrations resulted in decreased LH release and the proportion of cattle ovulating in response to GnRH treatment. There was no significant difference between heifers and cows in LH release or ovulatory response.

364 DISTRIBUTION OF OVULATION AND SUBSEQUENT EMBRYO PRODUCTION USING LUTROPIN AND ESTRADIOL-17β FOR TIMED AI OF SUPERSTIMULATED BEEF FEMALES

2006 ◽  
Vol 18 (2) ◽  
pp. 289 ◽  
Author(s):  
W. Larkin ◽  
P. Chesta ◽  
C. Looney ◽  
G. Bo ◽  
D. Forrest
Keyword(s):  
Animal Health ◽  
Transrectal Ultrasound ◽  
Beef Cows ◽  
Control Treatment ◽  
Embryo Production ◽  
Ultrasound Evaluation ◽  
Frozen Semen ◽  
Prostaglandin F ◽  
Estradiol 17Β ◽  
Post Onset

This experiment was designed to compare timing of ovulation and embryo production between traditional superstimulatory and AI methods and the use of additional treatments of Estradiol-17β and pLH (Lutropin; Bioniche Animal Health, Belleville, Ontario, Canada) to facilitate timed AI in beef cows. A total of 31 mature cross-bred beef cows were randomly assigned three treatments. On Day 0, experimental cows were selected upon transrectal ultrasound evaluation for corpus leutum (>10 mm) prior to CIDR insertion plus 2.5 mg estradiol-17β and 50 mg Progesterone i.m. Superstimulatory treatments with Folltropin-V (Bioniche Animal Health) began on Day 4 for 3.5 days (236 mg NIH-FSH-P10) in decreasing dosages (7 injections at 0700 and 1900). All cows received prostaglandin F (PGF) treatments of 625 mcg D-cloprostenol i.m. (Estrumate; Schering-Plough, Union, NJ, USA) in both AM and PM on Day 6, and CIDRs were removed in AM of Day 7, followed by the final injection of Folltropin. In addition, Heat Watch patches (Heat Watch; Cow Chips, Denver, CO, USA) were applied to optimize heat detection. Donors selected as control treatment were AI at 12 and 24 h post-onset of estrus (first mount) with frozen semen from the same bull. Donors selected on the Estradiol-17β treatment received 1 mg i.m. 12 h after CIDR removal and timed AI at 24 and 36 h. Donors selected for Lutropin treatment received 12.5 mg (5cc i.m.) 24 h after CIDR removal and were timed AI at 12 and 24 h. All AI procedures used 2 units from the same bull. All cows were examined by ultrasonography every 8 h beginning 24 h post-CIDR removal and ending at 60 h to determine the time and distribution of ovulation. Ovulation was determined by the disappearance of follicles (>12 mm) from the prior examination. All AI and ultrasound procedures were performed by the same technician. There were no differences detected between the three treatments as evaluated by all the variables we studied (Table 1). However, donors treated with Lutropin tended to produced more viable embryos per collection and had a tighter degree of distribution of ovulations. Donors treated with Estradiol-17β tended to have fewer viable embryos. More research is needed to determine if donors can be timed AI without regard to estrus. Table 1. Mean onset of estrus, distribution of ovulation, and embryo production in superstimulated beef donors treated with Estradiol-17β, and Lutropin

scholarly journals 143 SUPEROVULATORY RESPONSE AND EMBRYO PRODUCTION INFLUENCED BY THE ADDITION OF LH AND EFFECT OF THE REPEATABILITY IN SANTA INES SHEEP

2009 ◽  
Vol 21 (1) ◽  
pp. 171
Author(s):  
M. E. F. Oliveira ◽  
I. C. C. Santos ◽  
J. S. P. Pieroni ◽  
R. M. Ferreira ◽  
M. F. Cordeiro ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Ovulation Rate ◽  
Embryo Production ◽  
Chi Square ◽  
Pfizer Animal Health ◽  
Santa Inês ◽  
Intravaginal Device

The aim this study was to evaluate the effect of the addition of LH in superovulatory response and embryo production in Santa Inês sheep. Ten donors with 60.3 ± 10.7 kg and BCS of 3.9 ± 0.3 were superovulated in a cross-over design, with a 60-day interval. Estrus was synchronized with a progesterone-releasing intravaginal device (CIDR™; Pfizer Animal Health, Brazil) inserted on Day 0 and replaced by a new one on Day 7, that was maintained to Day 14. Two doses of 37.5 g of D-cloprostenol (Prolise™, Arsa, Buenos Aires, Argentina) were administered, on Days 7 and 14. Donors also receive 256 mg of pFSH (Folltropin™, Bioniche, Belleville, ON, Canada) in 8 decreasing doses, starting on Day 12. On Day 14, all females received 200 IU of eCG (Novormon ™, Syntex, Argentina). On Day 15, the animals were homogeneously allocated in 1 of 2 groups: Control (GC, n = 10) and treated (G-LH, n = 10). Ewes in GC did not receive exogenous LH, whereas ewes in G-LH were treated with 7.5 mg of LH (Lutropin™, Bioniche), on Day 15. All females were inseminated by laparoscopy, with frozen–thawed semen, 42 and 48 h after CIDR removal. On Day 21, the embryos were surgically collected. The superovulatory response was classified in scores: (0) 4 or fewer CL; (1) between 5 and 10 CL, and (2) 11 or more CL. Means were compared using Kruskal-Wallis test and percentages using chi-square (P < 0.05). Most of donors (70%, 7/10) from G-LH presented a superovulatory response classified as score 2, and the remaining (30%, 3/10) as score 1, whereas, half of the controls were classified as score 2 and half as score 1. Ovulation rate tended to be greater in G-LH (135/158, 85.4% v. 105/135, 77.7%, P = 0.08). The number of CL (mean ± SD) was 10.5 ± 3.8 in GC and 13.5 ± 4.84 in G-LH, but was not statistically different. The number of anovulatory follicles (AF) did not differ between groups (GC: 3.0 ± 3.2; G-LH: 2.3 ± 1.6), but the proportion of AF tended to decrease in G-LH (30/135, 22.2% v. 23/158, 14.5%, P = 0.08). Considering embryo production, there was no difference between GC and G-LH (P > 0.05) related to number of recovered ova/embryos (6.1 ± 4.6 v. 8.4 ± 5.2), viable embryos (3.8 ± 4.3 v. 4.2 ± 5.2), unfertilized (1.7 ± 3.4 v. 2.0 ± 2.9) and degenerated embryos (0.7 ± 0.7 v. 2.2 ± 2.9), respectively. Data showed that the addition of LH tended to increase ovulation rate and to decrease the proportion of AF, but did not affect the number of viable embryos.

126 A Comparison of Two Different Follicular Coasting Periods for In Vitro Embryo Production in Indian Nelore Cattle

2018 ◽  
Vol 30 (1) ◽  
pp. 203
Author(s):  
P. Tiwari ◽  
S. Zawar ◽  
J. H. Pryor ◽  
C. R. Looney ◽  
R. Kaushik ◽  
...  
Keyword(s):  
Animal Health ◽  
Bos Indicus ◽  
Embryo Production ◽  
Treatment Groups ◽  
Lactating Cows ◽  
Oocyte Recovery ◽  
Minimum Interval ◽  
Nelore Cattle ◽  
Donor Cows

Ongole, also known as Nelore (Bos indicus) cattle, are indigenous to the Andhra region in the Prakasam District in the State of Andhra Pradesh in India. A better understanding and utilisation of follicular wave dynamics within this breed would ultimately enhance oocyte and potential embryo production. Therefore, the aim of this study was to evaluate the differences between coasting periods of 24 h (S1) and 36 h (S2) on oocyte recovery, the rate of viable oocytes, cleavage, and Day 7 blastocyst rates of Nelore cattle in India. A total of 58 ovum pick-up (OPU) sessions (29 per treatment) were performed on 32 healthy donor cows that were randomly assigned to 1 of 2 coasting treatments (S1 or S2). Donors were stimulated as follows: 2.5 mL of gonadotropin-releasing hormone (GnRH; Receptal, MSD Animal Health, New Zealand) given IM on Day 1 followed by once-daily descending dose of Folltropin® (FSH, Vetoquinol, Canada) on Days 3 to 5 for a total of 180 mg. Cumulus-oocyte complexes were collected following OPU on Day 6 either at 24 (S1) or 36 h (S2) following the last FSH injection (coasting period). Donors were subject to OPU 1 to 3 times with a minimum interval of 15 days between procedures from March to April 2017. All 32 donor cows were non-lactating at the time of aspiration and divided equally between treatment groups. A total of 1492 follicles produced 850 total oocytes with oocyte recovery numbers for treatments S1 and S2 (785, 707; 441, 409; respectively). All data were analysed by ANOVA (P < 0.05). The mean number of follicles aspirated from S1 (27 ± 20.2) was not significantly different from that of S2 (24.4 ± 14.4). For S1, 393/441 (89%) quality oocytes were utilised for culture compared with 323/409 (78.9%) for S2, with no differences between rates. Additionally, there were no differences between mean number of oocytes, cleaved embryos, and blastocysts for S1 (15.2 ± 12.7; 9.9 ± 9.2; 4.3 ± 5.4) and S2 (14.1 ± 10; 7.4 ± 6.0; 3.6 ± 3.3; respectively). In conclusion, there were no differences found between 24- or 36-h coasting periods of Nelore cattle undergoing OPU for follicle counts, oocyte recovery, viable oocyte rates, cleavage, and blastocyst rates. Further research is needed to determine whether different stimulation protocols, the use of lactating cows, or coasting periods could alter outcomes.

scholarly journals 18 STRATEGIES TO IMPROVE FERTILITY WITH COSYNCH-CIDR PROTOCOLS IN BEEF CATTLE

2005 ◽  
Vol 17 (2) ◽  
pp. 159 ◽  
Author(s):  
R. Mapletoft ◽  
M. Colazo ◽  
L. Siqueira ◽  
J. Small ◽  
M. Rutledge ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Animal Health ◽  
Beef Cows ◽  
Preovulatory Follicle ◽  
Beef Heifers ◽  
Treatment Control ◽  
Pfizer Animal Health ◽  
Follicle Size ◽  
D 12 ◽  
To Receive

Two experiments were designed to evaluate strategies to improve fertility with Cosynch-CIDR protocols in cattle. The first experiment investigated the effect of low levels of progesterone prior to a Cosynch-CIDR protocol. On Day 0, lactating beef cows (n = 34) and heifers (n = 37) were placed in two groups to receive 500 μg cloprostenol (PGF; Estrumate, Schering-Plough Animal Health, Pointe-Claire, Quebec, Canada) or a twice-used CIDR (Bioniche Animal Health, Belleville, Ontario, Canada) for 5 days (Pretreatment) or no treatment (Control). On Day 5, used CIDRs were removed and all cattle received a new CIDR (Pfizer Animal Health, Montreal, Quebec, Canada) and 100 μg GnRH (Cystorelin, Merial Canada Inc, Victoriaville, Quebec, Canada). On Day 12, CIDR were removed and PGF was given. A second GnRH was given concurrent with timed AI (TAI) on Day 14 (54–56 h after PGF). Cattle were examined by transrectal ultrasonography for CL and follicle development, and for confirmation of pregnancy (Days 42 to 49). Diameter of the dominant follicle on Day 5 was larger and more variable in cows than in heifers (15.5 ± 5.6 vs. 11.4 ± 3.5 mm, respectively; means, P < 0.001; variance, P < 0.003), and tended to be larger in the Pretreatment group (14.3 ± 4.9 vs. 12.6 ± 5.2 mm; P = 0.13). More Pretreated (60.0%) than Control (36.1%) cattle (P < 0.005), and more cows (64.7%) than heifers (32.4%; P < 0.03) ovulated following the first GnRH. At the time of TAI, 5 (13.5%) heifers had already ovulated (P < 0.03). Diameter of the preovulatory follicle was affected by parity (P < 0.001), but not Pretreatment (P = 0.4), and tended to be larger in heifers that became pregnant (P = 0.13). Pregnancy rate was not affected by parity (P = 0.71), or pretreatment (P = 0.34); pretreatment tended to increase pregnancy rate in heifers (63.2 vs. 38.9%; P = 0.19). The second experiment evaluated the use of eCG in a Cosynch-CIDR protocol in beef heifers. Beef heifers (n = 127) were fed 0.5 mg/head/day of MGA (Pfizer Animal Health) for 15 d; 12 d after the last feeding (designated as Day 0) heifers received a CIDR and 100 μg GnRH. On Day 7, CIDR were removed, and heifers received PGF, and were randomly placed in 2 groups to receive 300 IU of eCG (Pregnacol; Bioniche Animal Health) or no treatment (Control). On Day 9 (54–56 h after PGF), all heifers received 100 μg GnRH, concurrent with TAI. Ultrasonographic examinations were done as in the first experiment. Overall, 79.5% of the heifers had a CL, and 9.4% had a luteinized follicle on Day 0. Seventy-eight heifers (61.4%) ovulated following the first GnRH, and those that ovulated had a less variable preovulatory follicle size than those that did not (13.7 ± 1.7 vs. 13.8 ± 2.3 mm; means, P = 0.76; variance, P < 0.01). However, there was no difference in preovulatory follicle size (P = 0.63), or pregnancy rate (49.2 vs. 53.1%; P = 0.7) for eCG-treated vs. Control heifers. In summary, pretreatment with a twice-used CIDR plus PGF increased the proportion of cattle that ovulated to the first GnRH, but not preovulatory follicle size or fertility in cows; fertility tended to be improved in heifers. Treatment with eCG did not increase preovulatory follicle size or fertility in heifers subjected to an Cosynch-CIDR protocol.

11 EFFECTS OF CIDR-BASED PRESYNCHRONIZATION AND eCG ON FERTILITY FOR A GnRH-BASED TIMED-AI PROTOCOL IN BEEF CATTLE

2006 ◽  
Vol 18 (2) ◽  
pp. 114 ◽  
Author(s):  
M. Colazo ◽  
J. Small ◽  
J. Kastelic ◽  
H. Davis ◽  
D. Ward ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Factorial Design ◽  
Pregnancy Rate ◽  
Animal Health ◽  
Beef Cows ◽  
Beef Heifers ◽  
Treatment Control ◽  
Pfizer Animal Health ◽  
Exogenous Progesterone ◽  
To Receive

The objectives were to determine the effects of presynchronization and eCG on fertility for a GnRH-based timed-AI (TAI) protocol in beef cattle. Previously autoclaved once-used CIDR inserts (Colazo et al. 2004 Anim. Reprod. Sci. 81, 25-34) were used for experimental purposes so that all cattle had equivalent exogenous progesterone after Day 7 in Experiment 1, and to induce GnRH-responsive follicles in Experiment 2. In Experiment 1, 12-15 month old beef heifers (n = 447) were used (three locations, A, B, and C). On Day 0, half received a new CIDR insert (Pfizer Animal Health, Montreal, Quebec, Canada) and 500 �g of cloprostenol i.m. (PGF: Estrumate; Schering-Plough Animal Health, Pointe-Claire, Quebec, Canada). On Day 7, the remainder received an autoclaved once-used CIDR and all received 100 �g of GnRH i.m. (Cystorelin; Merial Canada, Inc., Victoriaville, Quebec, Canada). On Day 14, CIDR inserts were removed, PGF was given to all heifers, and heifers were given either 300 IU eCG i.m. (Pregnacol; Bioniche Animal Health, Belleville, Ontario, Canada) or no treatment (2 � 2 factorial design). On Day 16 (54-56 h after CIDR removal and PGF administration), heifers were given 100 �g of GnRH i.m., concurrent with TAI. Transrectal ultrasonography was done on Day 0 to assess ovarian structures, and on approximately Day 50 (range, 44-69) to confirm pregnancy. Overall, 72.7% of heifers were puberal on Day 0 (150/150, 5/37, and 170/260 for locations A, B, and C, respectively; P < 0.001). Pregnancy rate was affected (P < 0.001) by location (27.3, 62.2, and 51.1% for the three locations, respectively) but was not affected (P = 0.3) by puberal status at locations B and C. Pregnancy rate was not affected (P = 0.5) by presynchronization, but tended (P = 0.1) to be reduced by eCG treatment (40.4 vs. 47.7%, respectively). In Experiment 2, lactating crossbred beef cows (n = 411), 2-12 years of age were randomly allocated to receive either a previously used, autoclaved CIDR for 7 days and 25 mg of dinoprost i.m. (PGF: Lutalyse; Pfizer Animal Health) or no treatment (control; Day 0). At CIDR removal (Day 7), all cows received 100 �g of GnRH. On Day 14, all cows received PGF and were allocated to receive either 400 IU of eCG i.m. or no treatment (2 � 2 factorial design). On Day 16 (54-56 h after PGF), cows were given 100 �g of GnRH concurrent with TAI. Transrectal ultrasonographic examinations were done on approximately 65 cows in each group on Days 0, 7, and 14 to assess ovarian structures and on all cows on Day 42 to confirm pregnancy. Overall, 88.8% of cows had a corpus luteum (CL) on Day 0. Presynchronization increased both the proportion of cows that ovulated to GnRH treatment on Day 7 (76.7 vs. 55.0%; P < 0.001) and pregnancy rate (58.2 vs. 45.4%; P = 0.03) for cows that had received a CIDR vs. control cows. However, eCG treatment did not affect pregnancy rate (P = 0.3) in either group. In summary, presynchronization with a used CIDR and PGF prior to a Cosynch protocol increased ovulation rate to the first GnRH treatment and pregnancy rate in cows but not in heifers. In a GnRH-based TAI protocol, treatment with eCG had no significant effect on fertility in cows, but tended to decrease fertility in heifers.

27 FOLLICULAR RESPONSE TO DIFFERENT eCG DOSES IN AN OVSYNCH + PROGESTERONE PROTOCOL IN BEEF HEIFERS

2010 ◽  
Vol 22 (1) ◽  
pp. 171
Author(s):  
M. F. Martínez ◽  
D. Tutt ◽  
L. Proctor ◽  
J. L. Juengel
Keyword(s):  
New Zealand ◽  
Animal Health ◽  
Follicular Development ◽  
Dominant Follicle ◽  
Treatment Groups ◽  
Follicular Wave ◽  
Multiple Ovulation ◽  
Array Transducer ◽  
Pfizer Animal Health ◽  
Follicular Response

An experiment was designed to evaluate the effect of different doses of eCG on ovarian follicular dynamics in heifers treated with a Ovsynch plus progesterone protocol. Twenty-five cyclic yearling Black Angus heifers (373.0 ± 35.7 kg), in 2 replicates, received an injection of 100 μg of GnRH (Ovurelin, Bomac Laboratories Ltd., Auckland, New Zealand) i.m. and an intravaginal progesterone device (1.38 g of progesterone; Eazi-Breed CIDR, Pfizer Animal Health, New Zealand) on Day 0 (beginning of the experiment), followed by 500 μg of cloprostenol (PGF, Estrumate, Intervet/Schering-Plough Animal Health, Auckland, New Zealand) i.m. on Day 7, and a second 100 μg of GnRH injection given i.m. on Day 9 (56 h after PGF). At the time of PGF treatment, heifers were randomly assigned to 5 treatment groups to receive 0, 300, 500, 700, or 1000 IU of eCG (Folligon, Intervet/Schering-Plough Animal Health) i.m. Heifers were monitored by ultrasonography (Aloka 900-SSD equipped with a 7.5-MHz linear-array transducer; Aloka, Tokyo, Japan) daily from Day 0 to 9 (GnRH), and then every 12 h until ovulation. Data were analyzed by one-way ANOVA or Kruskall-Wallis test, and means or ranks were compared with LSD or Wilcoxon rank sum tests, respectively. Because a replicate effect was observed (P < 0.05) in the size of the dominant follicle at the second GnRH and prior to ovulation, replicate effect was included in the analysis. The luteal area at PGF treatment was significantly greater (P < 0.01) in heifers that ovulated (750.0 ± 97 mm2) in response to the first GnRH treatment than in those that did not (301.6 ± 42.7 mm2). The diameter of the dominant follicle at the time of PGF treatment was also greater (P < 0.05) in ovulating (11.2 ± 0.4 mm) than in nonovulating (9.7 ± 0.5 mm) heifers. The interval from the first GnRH treatment to the emergence of the next follicular wave was longer (P = 0.50) and more variable in heifers that did not ovulate (2.9 ± 0.4 d; n = 27) than in those that ovulated (1.9 ± 0.2 d; n = 23). There was no effect (P < 0.37) of eCG on the interval from PGF to ovulation (86 ± 1.9 h). The number of ovulations after the second GnRH was higher (P = 0.01) in the group of heifers treated with 1000 IU of eCG (1.8 ± 0.4) than in the other groups (1.0 ± 0.0; 1.1 ± 0.1; 1.2 ± 0.1; 1.0 ± 0.1). There was an effect of day of follicular wave emergence on the number of ovulations (P < 0.01). Heifers with a wave emerging 1 to 3 days after the first GnRH (n = 37), had one ovulation (1.0 ± 0.0), whereas heifers with a wave emerging on Day 4 (3 out of 4 heifers) and Days 5 to 7 (n = 9), ovulated 2 or more follicles. In summary, the multiple ovulation effect occurred when eCG was given to heifers with a follicular wave emerging on or after Day 4, and was potentiated when heifers received 1000 IU of eCG. Although the dose of eCG given at the time of PGF treatment in an Ovsynch program has a significant effect on follicular development, the time of emergence of the dominant follicle appeared to be more important in the ovulation of preovulatory follicle/s after the eCG and the second GnRH treatment.

420 SUPERSTIMULATION OF ANGUS DONORS WITH A SINGLE INTRAMUSCULAR INJECTION OF FOLLTROPIN®-V

2010 ◽  
Vol 22 (1) ◽  
pp. 367 ◽  
Author(s):  
A. Tríbulo ◽  
H. Tríbulo ◽  
R. Tríbulo ◽  
D. Carballo Guerrero ◽  
P. Tríbulo ◽  
...  
Keyword(s):  
Single Injection ◽  
Animal Health ◽  
Treatment Schedule ◽  
Embryo Production ◽  
Release Formulation ◽  
Treatment Protocols ◽  
Single Intramuscular Injection ◽  
Effect Of Treatment ◽  
Estradiol 17Β ◽  
Donor Cows

Two experiments were designed to determine the superovulatory response of Angus donors treated with a single injection of Folltropin®-V diluted in a slow release formulation (SRF; Bioniche Animal Health Inc., Belleville, Ontario, Canada). Experiment 1 was designed to compare superovulatory response in Angus donor cows (n = 29 per group) treated with a single i.m. injection of Folltropin®-V diluted in SRF with cows treated using the traditional twice-daily i.m. injection treatment schedule. On Day 0, all cows received 5 mg of estradiol-17β plus 50 mg of progesterone and a Cue-Mate® (Bioniche Animal Health). On Day 4, cows were superstimulated with 400 mg of NIH-FSH-P1 Folltropin®-V in twice-daily decreasing doses over 4 days or in a single i.m. injection in the neck. The single injection was prepared by diluting the Folltropin®-V lyophilized powder in 1 mL of saline for injection and mixed with 9 mL of the SRF in the syringe immediately before administration. In the am and pm of Day 6, all cows received PGF2 and Cue-Mates® were removed in the pm. In the am of Day 8, cows received 12.5 mg of porcine LH (Lutropin®-V; Bioniche Animal Health) and were inseminated 12 and 24 h later. Ova/embryos were collected nonsurgically on Day 15 and evaluated following IETS recommendations. Means were compared between groups by t-test Mean (±SEM) number of ova/embryos and transferable embryos were 13.7 ± 2.1 and 7.1 ± 1.3 v. 12.3 ± 1.5 and 5.3 ± 0.8 for donors treated with the single v. twice-daily injections, respectively (P > 0.4). Experiment 2 was designed to confirm the results of Experiment 1 and to compare the effect of different dosages of Folltropin®-V on embryo production in Angus cows. Cows (n = 23) were superstimulated by 6 treatment protocols (2 × 3 factorial) in a crossover design (i.e. all cows received the 6 treatments and all treatments were represented on each collection day). Cows received the same treatments as cows in Experiment 1 except that the dosages of Folltropin®-V used were 200, 300, or 400 mg. Statistical analysis (ANOVA) revealed a significant effect of dosage of Folltropin®-V on embryo production, but there was no effect of treatment (i.e. single v. twice-daily injections; P > 0.2), nor was there a treatment by dosage interaction (P > 0.7). Mean (± SEM) number of ova/embryos and transferable embryos were 10.3 ± 0.9 and 5.4 ± 0.6 v. 11.3 ± 0.6 and 5.6 ± 0.5 for donors treated with the single or twice-daily injections, respectively (P > 0.2). Furthermore, the mean number of ova/embryos and transferable embryos were higher in cows treated with 400 mg (13.9 ± 1.1 and 6.5 ± 0.7) and 300 mg (12.0 ± 1.0 and 6.1 ± 0.7) of Folltropin®-V than those treated with 200 mg (6.6 ± 0.7 and 4.0 ± 0.5). In summary, superstimulation of Angus donor cows with a single i.m. injection of Folltropin®-V diluted in an SRF resulted in comparable embryo production to the traditional twice-daily i.m. administration of Folltropin®-V over 4 days. Although response did not differ between 300 and 400 mg, results suggest that 300 mg of Folltropin®-V given i.m. by a single or twice-daily injections is the most appropriate dose for Angus donor cows.

16 PROGESTERONE RELEASE PATTERNS FROM CUE-MATE IN COMPARISON TO OTHER INTRAVAGINAL PROGESTERONE-RELEASING DEVICES IN LACTATING DAIRY COWS

2007 ◽  
Vol 19 (1) ◽  
pp. 126 ◽  
Author(s):  
D. Rogan ◽  
M. F. Martinez ◽  
G. A. Bo ◽  
P. Chesta ◽  
F. Feresin ◽  
...  
Keyword(s):  
Los Angeles ◽  
Repeated Measures ◽  
Buenos Aires ◽  
Animal Health ◽  
Plasma Progesterone ◽  
Pfizer Animal Health ◽  
The Mean ◽  
Main Effects ◽  
To Receive ◽  
Mean Concentration

Various intravaginal progesterone-releasing devices are commercially available and each is impregnated with different amounts of progesterone. An experiment was designed to characterize plasma progesterone release profiles from Cue-Mate in comparison with other commercially available progesterone-releasing devices. Cycling, lactating Holstein cows (n = 60; BCS between 2.0 and 3.0 out of 5) from commercial dairy farms in Argentina were used in 2 replicates. All cows received 2 injections of D+ cloprostenol (PGF, Bioprost-D�; Biotay, Buenos Aires, Argentina) 14 days apart and were randomly assigned to one of four groups to receive intravaginal devices as follows: Cue-Mate (1.56 g progesterone; Bioniche Animal Health, A/Asia Pty, Armidale, Australia; n = 10), DIB (1 g progesterone; Syntex SA, Argentina; n = 10), CIDR with 1.9 g progesterone (Pfizer Salud Animal S.A., Buenos Aires, Argentina; n = 10), or CIDR with 1.38 g progesterone (Pfizer Animal Health, Groton, CT, USA; n = 10). All devices were inserted 24 hours after the second PGF and were left in place for 31 days. Blood samples were taken for progesterone analysis at 6-hour intervals for the first 24 hours, then daily for a week, and every 2 days for an additional 25 days. Plasma progesterone concentrations were measured with a modified human double-antibody RIA kit (DPC Coat-A-Count�; Diagnostic Products Corporation, Los Angeles, CA, USA). Cows were observed twice daily to confirm that vaginal inserts were still in place. Time-series hormone data were analyzed using the MIXED procedure for repeated measures (SAS Institute, Inc., Cary, NC, USA). Main effects of device and time, the highest mean concentration of progesterone, and the mean day on which this occurred were compared by a protected LSD test. There was no evidence of cow discomfort with any of the vaginal devices, and loss rates did not differ. There was an effect of group (P &lt; 0.001) and day (P &lt; 0.001) for progesterone concentrations, but there was no interaction (P = 0.89). Peak concentrations of progesterone (ng mL-1) did not differ among groups (Cue-Mate: 5.0 � 1.1; DIB: 3.9 � 0.6; 1.9-g CIDR: 4.6 � 0.6; 1.38-g CIDR: 3.7 � 0.4; P = 0.51). Mean (� SEM) concentrations of progesterone over the 31-day insertion period differed between 1.9-g CIDR (1.8 � 0.2 ng mL-1) and DIB (1.5 � 0.1 ng mL-1, P &lt; 0.006), with Cue-Mate (1.5 � 0.2 ng mL-1) and 1.38-g CIDR (1.6 � 0.1 ng mL-1) intermediate and not different from either. When data were evaluated from insertion until Day 7, mean plasma progesterone concentrations did not differ among groups (Cue-Mate: 2.2 � 0.2 ng mL-1; DIB: 2.2 � 0.1 ng mL-1; 1.9-g CIDR: 2.6 � 0.2 ng mL-1; 1.38-g CIDR: 2.1 � 0.1 ng mL-1). However, progesterone concentrations from insertion until Day 21 were higher (P &lt; 0.03) in cows with a 1.9-g CIDR (2.1 � 0.1 ng mL-1) than in those with a DIB (1.6 � 0.1 ng mL-1) or a 1.38-g CIDR (1.6 � 0.1 ng mL-1), with Cue-Mate (1.7 � 0.1 ng mL-1) intermediate and not different. Based on progesterone release patterns, it was concluded that Cue-Mate intravaginal devices can be used in protocols for synchronization of estrus and ovulation as with other progesterone-releasing devices.

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