231 SUPEROVULATORY RESPONSE AND EMBRYO YIELD OF SANTA INES EWES SUBMITTED TO FOLLICLE STIMULATING HORMONE TREATMENTS STARTED NEAR THE TIME OF EMERGENCE OF THE FIRST AND LAST FOLLICULAR WAVE OF A PROGESTERONE-BASED PROTOCOL

2012 ◽  
Vol 24 (1) ◽  
pp. 228
Author(s):  
M. E. F. Oliveira ◽  
C. C. D'Amato ◽  
L. G. Oliveira ◽  
S. D. Bicudo ◽  
J. F. Fonseca ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Follicle Stimulating Hormone ◽  
Corpora Lutea ◽  
Chi Square ◽  
Follicular Waves ◽  
Follicular Wave ◽  
Embryo Yield ◽  
Santa Inês ◽  
Follicular Dynamics

This study was designed to investigate if the superovulatory response and embryo yield in Santa Ines ewes are influenced by the time that FSH treatments are inititiated: near the emergence of the first or last follicular wave of a progesterone-based (P4) protocol. Days of emergence of the follicular waves was defined in a previous study that evaluated follicular dynamics in oestrus synchronization treatments (Oliveira et al. 2011 Acta Sci. Vet. 40). Twenty Santa Ines cyclic ewes were submitted to 2 superovulatory protocols according to the time that FSH treatments were initiated (G-first wave, n = 10 and G-last wave, n = 10). Thus, the FSH treatment started on Day 4 and 10 of protocol for G-first and G-last, respectively (Day 0 = onset of protocol). Ewes were treated with a P4 device (CIDR®; Pfizer-New Zealand) for 6 and 12 days for G-first and G-last, respectively. Additionally, in G-last, the CIDR was replaced by a new one on Day 7. All ewes received 2 injections of 37.5 μg of D-cloprostenol (Prolise®, Arsa-Argentina) on Day 0 and at CIDR removal. The superovulatory regimen consisted of 8 IM injections of pFSH (Folltropin®; Bioniche Animal Health, Belleville, Ontario, Canada) administrated twice daily (40, 40, 30, 30, 20, 20, 10 and 10 mg of porcine FSH). A single IM dose of 200 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) was given concurrently with CIDR removal. Ewes were mated by a fertile ram. Embryo collections were accomplished 7 days after CIDR removal. The superovulatory response was classified in scores: (0) 4 or fewer corpora lutea (CL); (1) between 5 and 10 CL and (2) 11 or more CL. Means (± SD) were compared using Kruskal-Wallis test and percentages using Chi-square (P < 0.05). Most donors (70%, 7/10) from G-last had a superovulatory response classified as score 2 and the remainder (30%, 3/10) as score 1, whereas half of ewes from G-first were classified as score 2 and half as score 1 (P > 0.05).There was no effect between treatments (P > 0.05) for ovulation rate (G-first: 90.7 ± 10.2% vs G-last: 93.0 ± 7.2%), number of ovulations (G-first: 13.1 ± 7.3 vs G-last: 14.0 ± 5.8), or number of luteinized unovulated follicles (G-first: 1.4 ± 1.3 vs G-last: 0.9 ± 1.0). Furthermore, there was no effect between G-first and G-last (P > 0.05) in the rate of ova/embryos recovered (55.1 ± 27.1% vs 67.0 ± 19.4%), mean number of ova/embryos recovered (7.0 ± 5.5 vs 9.1 ± 3.8), number of viable embryos (3.9 ± 6.1 vs 5.7 ± 4.4), or viability rate (49.1 ± 43.7 vs 58.5 ± 34.6). In conclusion, superovulatory response and embryo yield did not differ between FSH treatments initiated during the first or last follicular wave of a progesterone-based treatment in Santa Ines ewes. Financial support of FAPESP is acknowledged.

scholarly journals 317 EFFECT OF TIME OF ONSET OF FOLLICLE-STIMULATING HORMONE TREATMENT ON SUPEROVULATORY RESPONSE AND EMBRYO YIELD OF SANTA INES EWES DURING BREEDING SEASON

2013 ◽  
Vol 25 (1) ◽  
pp. 306
Author(s):  
M. E. F. Oliveira ◽  
C. C. D'Amato ◽  
C. S. Oliveira ◽  
F. F. P. C. Barros ◽  
A. P. Perini ◽  
...  
Keyword(s):  
New York ◽  
Breeding Season ◽  
Animal Health ◽  
Hormone Treatment ◽  
Corpora Lutea ◽  
Mating Period ◽  
Follicular Wave ◽  
Embryo Yield ◽  
Pfizer Animal Health ◽  
Santa Inês

This study was designed to investigate if the time of onset of FSH treatment [near the emergence of first or last follicular wave on progesterone (P4) protocol] influenced the superovulatory response and embryo yield in Santa Ines ewes during breeding season. Days of emergence of the follicular waves were defined in a previous study that evaluated the follicular dynamic in oestrus synchronization treatments (Oliveira et al. 2011 Acta Sci. Vet. 40). We observed emergence of the first and last follicular wave on 5.69 ± 0.42 and 11.25 ± 0.39 days of protocol, respectively. Twenty Santa Ines ewes were submitted to 2 superovulatory protocols according to the time that FSH treatments were initiated (G-first wave, n = 10; G-last wave, n = 10). On Day 0, all ewes received a P4 device (CIDR®; Pfizer Animal Health, New York, NY, USA) and injection of 37.5 µg of d-cloprostenol, IM. The FSH treatments started on Day 6 and Day 11 of protocol for G-first and G-last, respectively. The superovulatory regimen consisted of 8 IM injections of pFSH administrated twice daily (40, 40, 30, 30, 20, 20, 10, and 10 mg of pFSH). The P4 device was removed on Day 8 and Day 13 for G-first and G-last, respectively. At these times, all ewes received another injection of 37.5 µg of d-cloprostenol and a dose of 200 IU of eCG. During 4 days after the P4 device removal, ewes were mated by a fertile ram. Embryo collections were accomplished 7 days after CIDR withdrawal. The ovaries were evaluated by ultrasonography (3 times daily, during the mating period) and laparotomy (concomitantly with embryo collection). The superovulatory response was observed by classified by score: 0 = 4 or fewer corpora lutea (CL); 1 = between 5 and 10 CL; and 2 = 11 or more CL. Data were analysed by GLIMMIX using SAS software (SAS Institute Inc., Cary, NC, USA). All donors from G-first had superovulatory response classified as score 2, whereas 60% of ewes from G-last were classified as score 2, 20% as score 1, and 20% as score 0 (P < 0.05). There were effects between treatments (P < 0.05) for ovulation rate (G-first: 97.9 ± 1.4% v. G-last: 88.5 ± 4.4%) and number of ovulations (G-first: 17.0 ± 2.3 v. G-last: 12.5 ± 2.6). The numbers of luteinized unovulated follicles were 0.7 ± 0.5 for G-first and 1.2 ± 0.4 for G-last (P > 0.05). There was no difference between G-first and G-last (P > 0.05) in the rate of ova/embryos recovered (54.9 ± 5.7% v. 49.3 ± 8.5%), mean number of ova/embryos recovered (9.0 ± 1.4 v. 6.3 ± 1.1), number of viable embryos (3.8 ± 1.5 v. 3.4 ± 0.8), or viability rate (40.3 ± 10.8 v. 53.4 ± 12.1). In conclusion, the FSH treatment started near the emergence of the first follicular wave of progesterone protocol in Santa Ines ewes during the breeding season resulted in a higher superovulatory response than that started near the last follicular wave; however, no improvements in embryo yield were observed. Financial support: FAPESP.

scholarly journals 143 SUPEROVULATORY RESPONSE AND EMBRYO PRODUCTION INFLUENCED BY THE ADDITION OF LH AND EFFECT OF THE REPEATABILITY IN SANTA INES SHEEP

2009 ◽  
Vol 21 (1) ◽  
pp. 171
Author(s):  
M. E. F. Oliveira ◽  
I. C. C. Santos ◽  
J. S. P. Pieroni ◽  
R. M. Ferreira ◽  
M. F. Cordeiro ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Ovulation Rate ◽  
Embryo Production ◽  
Chi Square ◽  
Pfizer Animal Health ◽  
Santa Inês ◽  
Intravaginal Device

The aim this study was to evaluate the effect of the addition of LH in superovulatory response and embryo production in Santa Inês sheep. Ten donors with 60.3 ± 10.7 kg and BCS of 3.9 ± 0.3 were superovulated in a cross-over design, with a 60-day interval. Estrus was synchronized with a progesterone-releasing intravaginal device (CIDR™; Pfizer Animal Health, Brazil) inserted on Day 0 and replaced by a new one on Day 7, that was maintained to Day 14. Two doses of 37.5 g of D-cloprostenol (Prolise™, Arsa, Buenos Aires, Argentina) were administered, on Days 7 and 14. Donors also receive 256 mg of pFSH (Folltropin™, Bioniche, Belleville, ON, Canada) in 8 decreasing doses, starting on Day 12. On Day 14, all females received 200 IU of eCG (Novormon ™, Syntex, Argentina). On Day 15, the animals were homogeneously allocated in 1 of 2 groups: Control (GC, n = 10) and treated (G-LH, n = 10). Ewes in GC did not receive exogenous LH, whereas ewes in G-LH were treated with 7.5 mg of LH (Lutropin™, Bioniche), on Day 15. All females were inseminated by laparoscopy, with frozen–thawed semen, 42 and 48 h after CIDR removal. On Day 21, the embryos were surgically collected. The superovulatory response was classified in scores: (0) 4 or fewer CL; (1) between 5 and 10 CL, and (2) 11 or more CL. Means were compared using Kruskal-Wallis test and percentages using chi-square (P < 0.05). Most of donors (70%, 7/10) from G-LH presented a superovulatory response classified as score 2, and the remaining (30%, 3/10) as score 1, whereas, half of the controls were classified as score 2 and half as score 1. Ovulation rate tended to be greater in G-LH (135/158, 85.4% v. 105/135, 77.7%, P = 0.08). The number of CL (mean ± SD) was 10.5 ± 3.8 in GC and 13.5 ± 4.84 in G-LH, but was not statistically different. The number of anovulatory follicles (AF) did not differ between groups (GC: 3.0 ± 3.2; G-LH: 2.3 ± 1.6), but the proportion of AF tended to decrease in G-LH (30/135, 22.2% v. 23/158, 14.5%, P = 0.08). Considering embryo production, there was no difference between GC and G-LH (P > 0.05) related to number of recovered ova/embryos (6.1 ± 4.6 v. 8.4 ± 5.2), viable embryos (3.8 ± 4.3 v. 4.2 ± 5.2), unfertilized (1.7 ± 3.4 v. 2.0 ± 2.9) and degenerated embryos (0.7 ± 0.7 v. 2.2 ± 2.9), respectively. Data showed that the addition of LH tended to increase ovulation rate and to decrease the proportion of AF, but did not affect the number of viable embryos.

36 FOLLICULAR DYNAMICS IN PROLIFIC SHEEP: PGF-BASED ESTRUS SYNCHRONIZATION

2010 ◽  
Vol 22 (1) ◽  
pp. 176
Author(s):  
L. Proctor ◽  
D. Tutt ◽  
D. Olliver ◽  
S. Galloway ◽  
J. L. Juengel ◽  
...  
Keyword(s):  
Animal Health ◽  
T Test ◽  
Corpora Lutea ◽  
Follicular Wave ◽  
Follicle Diameter ◽  
Array Transducer ◽  
Significant Difference ◽  
Preovulatory Follicles ◽  
Student’S T ◽  
Follicular Dynamics

A study was designed to compare the effect of a prostaglandin-based synchronization protocol on ovarian follicular dynamics in sheep with the FecB (Booroola) mutation. Forty dry Romney sheep (57.6 ± 7.3 kg; 6.1 ± 1.1 years) were randomly selected from both Invermay Booroola (BB; n = 20) and commercial (non-FecB carriers, ++; n = 20) flocks. All ewes had their estrous cycles synchronized with 2 i.m. injections of PGF (150 μg of cloprostenol, Estrumate, Schering-Plough Coopers Animal Health Ltd., New Zealand) administered 7 days apart. Ewes were monitored by transrectal ultrasonography (Aloka 900-SSD and a 7.5-MHz linear-array transducer, Aloka, Tokyo, Japan) daily from Day -2 to the day of ovulation. Data were analyzed by Student’s t-test or Wilcoxon Rank Sum test. Variances were compared with Barlett’s test. Paired t-test compared the number of preovulatory follicles in each genotype after PGF treatments and intervals from PGF to ovulation after PGF. Data are presented as mean (± SEM). The number of corpora lutea (CL) and total CL area at the time of the first and second PGF treatment were 4.4 ± 0.6; 5.7 ± 1.4 and 672.1 ± 133.5 mm2; 999.0 ± 145.9 mm2 in the BB and 2.1 ± 0.2; 2.1 ± 0.5 and 342.3 ± 60.7 mm2; 401.3 ± 68.6 mm2 in ++ ewes, respectively. These 2 variables were higher (P < 0.01) at both PGF injections in the BB than in the ++ ewes, except the CL area at the time of first PGF treatment (P = 0.15). The largest follicle diameter at the time of the first and second PGF treatments was smaller (P < 0.003) in BB (4.1 ± 0.3 mm; 3.5 ± 0.2 mm) than in ++ (5.3 ± 0.3 mm; 5.8 ± 0.1 mm) ewes. The median and mean number of follicles that ovulated after the first and second PGF treatment were higher (P < 0.0001) in BB (6 & 7; 5.7 ± 0.3; 6.9 ± 0.3; difference = 1.2 ± 0.4; P < 0.003) than in the ++ (2 & 2; 2.1 ± 0.1; 2.1 ± 0.1) sheep. The luteal area at the time of first and second PGF in both BB and ++ did not differ (P = 0.3). The intervals from the first and second PGF to the respective ovulations did not differ (P > 0.61) between BB (3.4 ± 0.2; 3.0 ± 0.3d) and ++ (3.5 ± 0.2d; 3.0 ± 0.1d) ewes. However, interval from the second PGF to ovulation was more variable (P = 0.002) in the BB than in the ++ ewes. Data of both groups were combined and a mean significant difference of 0.6 ± 0.2d (P < 0.003) was found between the first and second PGF-to-ovulation intervals. The interval from the first PGF to emergence of the next follicular wave was shorter (P < 0.02) and more variable (P < 0.03) in the BB (2.7 ± 0.4d) than in the ++ (3.5 ± 0.2 d) group. Preovulatory follicles were smaller in Booroola, but higher in number, than in ++ ewes, whereas the luteal area was similar. Within the BB ewes, the higher number of follicles that ovulated after the second PGF than after the first injection may be due to a higher follicular response to an elevated rebound in circulating FSH after the first PGF. A high number of growing follicles of the first follicular wave may also have contributed to this event. These findings warrant further research aimed at the study of the interaction between FSH and follicle dynamics in estrus synchronized sheep carrying the FecB mutation.

418 EFFECT OF SUPEROVULATION INITIATED AT FOLLICULAR WAVE EMERGENCE IN Santa Inês EWES

2010 ◽  
Vol 22 (1) ◽  
pp. 366
Author(s):  
B. D. M. Silva ◽  
T. A. S. N. Silva ◽  
L. Dalcin ◽  
C. M. Lucci ◽  
J. P. Neves
Keyword(s):  
Total Dose ◽  
Buenos Aires ◽  
Ovarian Response ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Treatment Groups ◽  
Follicular Wave ◽  
Preliminary Study ◽  
Santa Inês ◽  
Fresh Semen

Superovulation in ewes has been a source of many studies aimed at obtaining high superovulatory response and number of viable embryos recovered. In a protocol called Day 0, superovulatory treatment was initiated at the time of wave emergence in the absence of a dominant follicle (Menchaca A et al. 2002 Theriogenology 58, 1713-1721). The aim of this study was to compare ovarian response and number of embryos recovered after treatment between ewes treated with a Day 0 protocol and those treated with a traditional protocol. Santa InÊs ewes (n= 18) between 2 and 5 years old were randomly distributed into 2 superovulation treatment groups: traditional protocol and Day 0 protocol. Each treatment was repeated twice in a crossover model. The traditional protocol consisted of the intravaginal insertion of a sponge containing 60 mg of medroxiprogesterone acetate (MAP; Progespon®, Tecnopec, São Paulo, Brazil) for 14 days, which was replaced on Day 7, followed by 150 μg of cloprostenol (Prolise®, Tecnopec) i.m. On Day 12, FSH (Folltropin®-V, Bioniche, Belleville, Ontario, Canada) treatment was initiated using a total dose of 200 mg, given in twice-daily i.m. injections that decreased in dose over 4 days. A dose of 200 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) i.m. was given at the time of sponge withdrawal. The AI was done by laparoscopy at 48 and 55 h after sponge withdrawal using fresh semen. The Day 0 protocol consisted of a previous 9-day synchronization treatment with a sponge containing 60 mg of MAP, followed by 150 μ of cloprostenol i.m. and 200 IU of eCG i.m. given on Day 7. A dose of 0.05 mg of GnRH (lecirelin; Gestran Plus®, Tecnopec) i.m. was given 16 h after sponge withdrawal. In a preliminary study, 38 ewes ovulated 42 ± 52 h after sponge withdrawal. Therefore, 48 h after sponge removal was considered as Day 0 and FSH treatment was initiated at that time, with a total dose of 200 mg of Folltropin®-V, given in 6 twice-daily decreasing doses. Two doses of cloprostenol (150 μg) were given i.m. concurrent with the fifth and sixth FSH treatments. Gonadotropin-releasing hormone (0.05 mg of lecirelin i.m.) was given 12 h after the last FSH treatment. Artificial insemination with fresh semen was done by laparoscopy 16 and 26 h after GnRH treatment. Five days after AI, embryos were recovered surgically. Results were evaluated by the parametric t-test. The number of corpora lutea and ova/embryos recovered did not differ (P > 0.05) between the traditional (9.8 ± 5.3; 4.5 ± 4.6) and Day 0 (10.0 ± 6.0; 3.5 ± 4.3) protocols. Similarly, no difference in the number of viable embryos was observed between treatments (1.6 ± 2.0 and 1.7 ± 2.4 for the traditional and Day 0 treatments, respectively). Within viable embryos, the traditional protocol (0.4 ± 1.0) resulted in ahigher (P < 0.05) number of morulae than that of the Day 0 protocol (0.1 ± 0.3). The ewes that had no superovulatory response did not differ (P > 0.05) between the traditional (11.11%) and Day 0 (5.56%) protocols. In summary, there was no difference in the parameters evaluated between both protocols. Financial support: FAPDF, CNPq, Tecnopec.

scholarly journals 14OVARIAN FOLLICULAR DYNAMICS IN COWS TREATED WITH A CIDR, ESTRADIOL AND PROGESTERONE LATE IN THE ESTROUS CYCLE

2004 ◽  
Vol 16 (2) ◽  
pp. 129 ◽  
Author(s):  
J.P. Kastelic ◽  
M.G. Colazo ◽  
J.A. Small ◽  
D.R. Ward ◽  
R.J. Mapletoft
Keyword(s):  
Estrous Cycle ◽  
Ovarian Follicle ◽  
Animal Health ◽  
Beef Cows ◽  
Control Group ◽  
Dominant Follicle ◽  
Chi Square ◽  
Follicular Wave ◽  
Follicle Diameter ◽  
Follicular Dynamics

The objective was to characterize ovarian follicular dynamics in beef cows treated with a CIDR (Bioniche Animal Health; Belleville, Ontario, Canada) and an injection of estradiol-17β (E2), with or without progesterone (P4), late in the estrous cycle. Previously synchronized, non-lactating, crossbred beef cows (n=36) received a CIDR (Day 0) 16 to 18 days after ovulation and were randomly allocated to one of three treatment groups: no further treatment (Control, n=12), an injection of 5mg E2 (E2, n=12), or 5mg E2 plus 100mg P4 (E2P4, n=12; both from Sigma Chemical Co., St.Louis, MO, USA) i.m. in 2mL canola oil. On Day 7, CIDR were removed and cows received 500μg i.m. of cloprostenol (Estrumate, Schering Plough Animal Health, Pointe-Claire, Quebec, Canada). Ovaries were examined once daily by transrectal ultrasonography to detect ovarian follicle growth profiles, and determine the time of ovulation. Blood samples were taken daily for progesterone determination. Data were analyzed by ANOVA (LSD and Bartlett’s tests), Student’s t-test and chi-square procedures. Diameter of the CL and the dominant follicle, and progesterone concentration on Day 0 did not differ among groups (P=0.6; overall mean (±SD), 16.8±2.7mm, 14.1±2.0mm, and 1.5±1.9ngmL, respectively). Thirteen cows ovulated within 3 days of treatment (50% of E2- and E2P4-treated cows and 8.3% of Control cows; P=0.05); cows that ovulated had smaller CL diameters (15.2±1.7 v. 17.7±2.7mm; P&lt;0.004) and lower progesterone concentrations (0.4±0.2 v.2.1±2.2ngmL; P&lt;0.001) at the time of treatment. Follicular wave emergence occurred within 7 days in 4/12 Control cows, 10/12 E2-treated cows, and 10/12 E2P4-treated cows (P&lt;0.01). Although the interval from treatment to wave emergence did not differ among treatments (P=0.8; overall, 3.4±1.5 days), follicular wave emergence was more synchronous (P&lt;0.004) in the E2 group than in the Control or E2P4 groups. At CIDR removal, dominant follicle diameter was larger (P&lt;0.02) in the Control group (15.9±5.5mm) than in the E2 (11.9±1.8mm) or E2P4 (11.5±3.4mm)groups, but dominant follicle diameter was less variable (P&lt;0.003) in the E2 group than in the other two groups. Three cows did not ovulate after CIDR removal; two in the Control group and one in the E2P4 group. Interval to ovulation was shorter (P&lt;0.05) in the Control group (70.8±10.5h)than in the E2 (87.0±9.0h) or E2P4 (86.2±7.2h) groups, and the intervals to ovulation in cows that ovulated following treatment (91.0±8.0h) was longer (P&lt;0.001) than in those that did not (76.6±9.6h). In summary, treatment of cows with an estradiol-progesterone protocol late in the estrous cycle resulted in ovulation (50.0%), atresia (33.3%) or persistence (16.6%) of the dominant follicle present at that time. As length of follicular dominance and timing of ovulation were affected, fertility may be impaired following AI.

Ovarian follicular dynamics and concentrations of oestradiol-17 beta, progesterone, luteinizing hormone and follicle stimulating hormone during the periovulatory phase of the oestrous cycle in the cow

1991 ◽  
Vol 3 (5) ◽  
pp. 529 ◽  
Author(s):  
H Kaneko ◽  
T Terada ◽  
K Taya ◽  
G Watanabe ◽  
S Sasamoto ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Follicular Phase ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Ovulatory Follicle ◽  
Follicular Dynamics ◽  
Stimulating Hormone

Changes in the plasma concentration of oestradiol-17 beta, progesterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) were characterized during the transition from the luteal to the follicular phase, the periovulatory period and the early luteal phase in five cycling cows. The pattern of growth and the regression of follicles and corpora lutea in the ovary of the same animals were also assessed by daily ultrasonographic examinations. Two waves of follicular growth (ovulatory and non-ovulatory) occurred in all animals. The ovulatory follicular wave started from 4 days before the preovulatory surges of LH and FSH and the wave of next growth of a dominant follicle (non-ovulatory follicle) started within one day after ovulation. Changes in plasma levels of oestradiol-17 beta correlated well with the growth of both ovulatory and non-ovulatory dominant follicles. Suppression of FSH concentration during the follicular phase was inversely related to the increase in plasma concentration of oestradiol-17 beta. These results suggest that, in the cow, ovulatory dominant follicles suppress FSH secretion by increasing the concentration of oestradiol-17 beta (and probably also inhibin) during the follicular phase.

scholarly journals The use of a ‘first-wave’ model to study the effect of nutrition on ovarian follicular dynamics and ovulation rate in the sheep

Reproduction ◽  
2010 ◽  
Vol 140 (6) ◽  
pp. 865-874 ◽  
Author(s):  
C Viñoles ◽  
B Paganoni ◽  
K M M Glover ◽  
J T B Milton ◽  
D Blache ◽  
...  
Keyword(s):  
Body Condition ◽  
Wave Model ◽  
Ovulation Rate ◽  
Short Term ◽  
Metabolic Hormones ◽  
Follicular Waves ◽  
Follicular Wave ◽  
The Third ◽  
High Concentrations ◽  
Follicular Dynamics

We have developed an experimental model in which groups of ewes are simultaneously experiencing the first ovarian follicular wave of their oestrous cycle. We used this ‘first-wave model’ in a 2×2 factorial experiment (ten ewes per group) to study the effect of body condition (BC) and a short-term supplement on follicular dynamics and ovulation rate. The ‘first-wave’ was established by giving ewes three injections of prostaglandin (PG), 7 days apart. The 6-day supplement (lupin grain) began 2 days after the second PG injection and continued until the third. Follicles were studied by ultrasound, and blood was sampled to measure glucose and hormones. The supplement increased (P<0.01) the concentrations of glucose, insulin and leptin, decreased FSH concentrations (P<0.01) and tended to increase oestradiol concentrations (P=0.06). The supplement tended to increase the number of 3 mm follicles (P=0.06). Compared with low-BC ewes, high-BC ewes had more follicular waves (P<0.05), higher concentrations of insulin, leptin and IGF1 (P<0.05) and tended to have higher FSH concentrations (P=0.09). Leptin and insulin concentrations remained high until the end of supplementation in high-BC ewes, whereas they decreased after the third day of supplementation in low-BC ewes. In conclusion, high concentrations of metabolic hormones in fat ewes are associated with the development of more follicular waves. When a supplement is superimposed on this situation, changes in glucose and metabolic hormones allow more follicles to be selected to ovulate.

174 EVALUATION OF THE 9-DAY PROTOCOL FOR ESTROUS SYNCHRONIZATION IN SANTA INÊS EWES

2011 ◽  
Vol 23 (1) ◽  
pp. 189
Author(s):  
P. Viau ◽  
M. B. Paes de Barros ◽  
L. M. K. Dias ◽  
S. S. Nicolau ◽  
C. T. Marino ◽  
...  
Keyword(s):  
Solid Phase ◽  
Device Removal ◽  
Intact Male ◽  
Estrous Synchronization ◽  
Follicular Wave ◽  
Vaginal Progesterone ◽  
Device Placement ◽  
Santa Inês ◽  
Veterinary Hospital ◽  
Follicular Dynamics

The objective of the present study was to characterise follicular dynamics in Santa Ines (SI) during a 9-day protocol for oestrous synchronization and to evaluate the efficiency of the vaginal progesterone-releasing device Primer-PR® (Tecnopec, Brazil). Cyclic females were used (n = 10) at the ruminant sector of the veterinary hospital of the FMVZ–USP, in March 2009. The animals had body scores between 2.5 and 3 and were of proven fertility. Ultrasound examinations were performed using an ALOKA SSD-500 Scanner (Berger, Brazil) with a linear 5.0-MHz transducer, attached to a handle to allow safe intrarectal manipulation. Examinations were performed daily from 3 days (D-3) before the day of device placement (D0) until the day of device removal (D9), and twice daily from device removal until ovulation. All ewes received 0.03 mg of prostaglandin (D-cloprostenol, Prolise®, Tecnopec, Brazil) on D9. Ovulation was assessed by the disappearance of the growing larger follicle or follicles present in the previous examinations. Oestrous detection was performed using an intact male 3 times a day (at 10:00 a.m., 6:00 p.m., and 12:00 p.m.) from D9 until the last acceptant ewe, and blood samples were taken by jugular puncture for progesterone (P4) measurement by radioimmunoassay on solid phase (COAT-A-COUNT, Siemens, USA) from D-3 until the day of ovulation. Data were analysed by Shapiro–Wilk (PROC UNIVARIATE) using the SAS program (SAS Institute Inc., Cary, NC, USA, 2001) and shown as mean ± standard deviation. Plasma P4 concentrations between D0 and D9 were 6.56 ± 2.32 ng mL–1, peaking between D3 and D5 (8.07 ± 2.31 ng mL–1). Oestrous behaviour was shown 45.6 ± 12.71 h after Primer-PR® removal. The first and the last ewe that showed oestrous behaviour was at 30 h and 66 h after Primer-PR® removal, respectively, and the majority of ewes (50%) at 42 h. Oestrous lasted 26.40 ± 9.47 h, and the majority of ewes (70%) showed oestrous behaviour during 24 h. Ovulation occurred 73 ± 14.38 h after Primer–PR® removal and 1.3 ± 0.48 ovulations per animal were observed. From all growing presumptively dominant follicles observed, 92.3% of them ovulated. When double ovulations occurred (n = 3), the interval between first and second ovulation was 16 ± 6.93 h. Emergence of the ovulatory follicular wave occurred at 8.5 days + 16 h after Primer–PR® insertion. The follicles observed to continue growing had a diameter of 3.48 ± 0.28 mm when they were first detected and reached 5.63 ± 0.66 mm, with a growth rate of 0.73 ± 0.43 mm per day. A standard follicle wave within the 9-day protocol was not possible to determine, and the follicles receded in up to 4 days. In conclusion, placement of a Primer-PR® device for 9 days resulted in synchronous oestrus and ovulation in Santa Inês ewes. FAPESP, CAPES, Tecnopec.

287 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION AND ABSENCE OF CORPUS LUTEUM ON COC RECOVERY IN Gyr (BOS TAURUS INDICUS) COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 300
Author(s):  
T. Miyauchi ◽  
C. A. C. Fernandes ◽  
E. R. Oliveira ◽  
B. F. L. Alves ◽  
J. H. M. Viana
Keyword(s):  
Bos Taurus ◽  
Animal Health ◽  
Cumulus Cell ◽  
Estradiol Benzoate ◽  
Corpora Lutea ◽  
Portable Ultrasound ◽  
Follicular Wave ◽  
Main Technique ◽  
Oocyte Donors

Transvaginal guided follicle aspiration (TGFA) is the main technique used in Brazil to recover oocytes for in vitro embryo production (IVP) in bovine. Different protocols have been proposed to synchronize follicular emergence in oocyte donors, but most of them were developed for use in European breeds of cattle, which show many differences in ovarian physiology when compared with Zebu breeds. The aim of this study was to compare different protocols for preparation of Gyr (dairy zebu breed) oocyte donors. The TGFA were performed in a donor management facility located in Minas Gerais State, southeast Brazil. Pluriparous cycling Gir cows (n = 42) were used as donors. All cows underwent 3 treatments: G1 (control), no treatment before TGFA; G2, 2 mg of estradiol benzoate (EB) for follicular wave synchronization given i.m. 5 days before TGFA; and G3, norgestomet auricular implants given 9 days and 2 mg of EB plus 0.53 mg of cloprostenol given 5 days before TGFA. The interval between TGFA in the same donor was greater than 30 days. All procedures were made by the same technician, using a portable ultrasound device, disposable 19G or 20G needles, and a vacuum pressure of 80 mm Hg. The aspirated follicular fluid was collected in 50-mL Falcon tubes and sent to the laboratory for COC identification and classification under 50 × magnification. Recovered oocytes were classified according to cumulus cell layers and cytoplasm morphology. The total number of oocytes and viable COC recovered and the procedure length (min), including time spent for TGFA and laboratory manipulation, were compared. Data were evaluated by ANOVA, and means compared by Tukey’s test. A total of 126 TGFA sessions were performed, with recovery of 2,809 oocytes (20.31 ± 12.32 of COC and 14.83 ± 7.97 of viable COC per cow/session; mean ± SD). The total number of recovered oocytes and viable COC recovered were lower in G1 compared with G2 and G3 (15.18 ± 11.07 v. 21.18 ± 9.71 and 24.68 ± 9.03; and 9.53 ± 7.22 v. 16.97 ± 6.47 and 18.84 ± 8.90, respectively; P < 0.05) There was no difference (P > 0.05) between G2 and G3 on the number of oocytes or viable COCs recovered. The procedure length, however, was longer in G1 and G2 compared with G3 (49.6 ± 15.1 and 46.9 ± 13.4 v. 35.8 ± 13.1 min, respectively; P < 0.05) The shorter procedure length in G3 was probably associated with the reduced number of cows showing no corpora lutea (38/42, 90%), which resulted in reduction of bleeding and clot formation in the aspirated fluid. These results show that (1) previous follicular wave synchronization by EB can improve the number and quality of recovered oocytes; (2) the absence of corpora lutea does not increase the number of recovered COC and viable COC, but reduces the time spent in the procedure. Intervet Schering Plough Animal Health.

295 SUPEROVULATORY RESPONSE IN COWS FOLLOWING SYNCHRONIZATION OF FOLLICLE WAVE EMERGENCE WITH ESTRADIOL AT DIFFERENT STAGES OF THE ESTROUS CYCLE

2008 ◽  
Vol 20 (1) ◽  
pp. 227
Author(s):  
A. Garcia Guerra ◽  
G. A. Bó ◽  
J. Villarreal ◽  
G. M. Brogliatti
Keyword(s):  
Estrous Cycle ◽  
Buenos Aires ◽  
Animal Health ◽  
Ovarian Response ◽  
Buenos Aires Province ◽  
Transfer Program ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Significant Difference ◽  
Group 2

Ovarian asynchrony and variability in response to superstimulation remain the most limiting factors in any embryo transfer program (Armstrong D 1993 Theriogenology 39, 7–24). Ovarian response can be increased and less variable if superstimulatory treatment is started at the time of follicular wave emergence (Bö GA et al. 1995 Theriogenology 43, 31–40). A combination of progesterone (P4) and estradiol have been used to synchronize follicular wave for superstimulation. A retrospective analysis was done to compare the ovarian response, superovulatory response and embryo production of cows in Argentina that received progesterone and estradol prior to superstimulation at different stages of the estrous cycle. This research was carried out using different breed of donors (n = 584, 88% Angus) during the last 4 years in Buenos Aires province, Argentina. Heat detection was performed twice a day. At random stages of the estrous cycle, donors received an intravaginal progesterone device (DIB; Syntex, Buenos Aires, Argentina), 2 mg of estradiol benzoate and 50 mg of progesterone (Syntex, Buenos Aires, Argentina) IM on the same day. On day 4 after DIB insertion, superestimulatory treatment was initiated on a decreasing dose regimen of FSH (Pluset; Callier, Spain, or Folltropin, Bioniche Animal Health Inc., Belleville, Ontario, Canada) as IM injections every 12 h over 4 d. On day 6, DIBs were removed, and cows received two doses of 2 mL of cloprostenol 12 h apart. At heat detection, all donors received a dose of 2 mL of GnRH (Dalmarelin; Fatro Von Franken, Buenos Aires, Argentina) by IM injection and were inseminated 12 and 24 h later. Seven days later, embryo collection was performed and ovarian response was evaluated as number of CL + unovulated follicles by transrectal ultrasound using a 7.5-MHz transducer (Pie Medical, Maastricht, the Netherlands). Ova/embryos were evaluated and classified according to the IETS manual. Donors were assigned to receive DIB and estradiol during the following stages of the cycle: group 1: between days 4 and 7 post-estrus (dominant follicle period), group 2: between days 8 and 12 post-estrus (emergence of the second follicular wave), and group 3: between days 13 and 21 post-estrus (dominant follicle of the second wave). Kruskal-Wallis test was used to compare variables among groups, and results are shown in Table 1. Ovarian response as CL + unovulated follicles and number of ovulations were significantly different among groups (P < 0.05). However, there was no significant difference in the number of fertilized ova or transferable embryos. Nevertheless, numeric differences that show that group 2 (started between days 8 and 12 post-estrus) was always superior for all variables. In conclusion, data suggest that estradiol may be more effective in synchronizing follicle wave emergence for superstimulation during the mid-part of the estrous cycle. Table 1. Superovulatory response in cows in which follicle wave emergence was synchronized with estradiol at different stages of the estrous cycle (mean ± SD) Research supported by Centro Genetico Bovino Eolia S.A.

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