scholarly journals Lipopolysaccharide O antigen size distribution is determined by a chain extension complex of variable stoichiometry in Escherichia coli O9a

2014 ◽  
Vol 111 (17) ◽  
pp. 6407-6412 ◽  
Author(s):  
J. D. King ◽  
S. Berry ◽  
B. R. Clarke ◽  
R. J. Morris ◽  
C. Whitfield
Keyword(s):  
Escherichia Coli ◽  
Size Distribution ◽  
Chain Extension ◽  
O Antigen ◽  
A Chain ◽  
Variable Stoichiometry

Spheroplasts of enteropathogenic Escherichia coli. I. Biological characteristics

1968 ◽  
Vol 14 (6) ◽  
pp. 675-678 ◽  
Author(s):  
B. Diena ◽  
R. Wallace ◽  
L. Greenberg
Keyword(s):  
Escherichia Coli ◽  
Tissue Culture ◽  
Biological Characteristics ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
O Antigen ◽  
Pathogenic Serotypes

The properties of glycine-induced spheroplasts of six pathogenic serotypes of E. coli were investigated. Fimbriae and flagella appeared to be only partially synthesized as was the somatic O antigen. Cytopathogenicity of these spheroplasts for tissue culture was reduced and the infection of the monolayers was retarded as compared with the normal bacillary forms. Sensitivity to phage was almost completely lost, suggesting that glycine had either interfered with the synthesis of phage receptors or had altered the mucopeptide layerwhich is the substrate for phage enzymes. Alternatively, the phage may become a prophage inside the spheroplast with the loss of virulence.

scholarly journals Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli

2005 ◽  
Vol 102 (8) ◽  
pp. 3016-3021 ◽  
Author(s):  
M. F. Feldman ◽  
M. Wacker ◽  
M. Hernandez ◽  
P. G. Hitchen ◽  
C. L. Marolda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Glycosylation ◽  
O Antigen

Structure and gene cluster of the O antigen of Escherichia coli F17, a candidate for a new O-serogroup

2019 ◽  
Vol 124 ◽  
pp. 389-395 ◽  
Author(s):  
Yuriy A. Knirel ◽  
Pavel A. Ivanov ◽  
Sofiya N. Senchenkova ◽  
Olesya I. Naumenko ◽  
Olga O. Ovchinnikova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cluster ◽  
O Antigen

Structural studies of the Escherichia coli O-antigen 25

1983 ◽  
Vol 122 (2) ◽  
pp. 249-256 ◽  
Author(s):  
Lennart Kenne ◽  
Bengt Lindberg ◽  
John K. Madden ◽  
Alf A. Lindberg ◽  
Peter Gemski
Keyword(s):  
Escherichia Coli ◽  
Structural Studies ◽  
O Antigen

scholarly journals P fimbriae and other adhesins enhance intestinal persistence of Escherichia coli in early infancy

1998 ◽  
Vol 121 (3) ◽  
pp. 599-608 ◽  
Author(s):  
I. ADLERBERTH ◽  
C. SVANBORG ◽  
B. CARLSSON ◽  
L. MELLANDER ◽  
L.-Å. HANSON ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Line ◽  
Intestinal Epithelial Cells ◽  
Antigen Expression ◽  
Intestinal Epithelial ◽  
E Coli ◽  
O Antigen ◽  
Ht 29 ◽  
Intestinal Persistence

Resident and transient Escherichia coli strains were identified in the rectal flora of 22 Pakistani infants followed from birth to 6 months of age. All strains were tested for O-antigen expression, adhesin specificity (P fimbriae, other mannose-resistant adhesins or type 1 fimbriae) and adherence to the colonic cell line HT-29. Resident strains displayed higher mannose- resistant adherence to HT-29 cells, and expressed P fimbriae (P=0·0036) as well as other mannose-resistant adhesins (P=0·012) more often than transient strains. In strains acquired during the first month of life, P fimbriae were 12 times more frequent in resident than in transient strains (P=0·0006). The O-antigen distribution did not differ between resident and transient strains, and none of the resident P-fimbriated strains belonged to previously recognized uropathogenic clones. The results suggest that adhesins mediating adherence to intestinal epithelial cells, especially P fimbriae, enhance the persistence of E. coli in the large intestine of infants.

scholarly journals Synthesis of polylactic acid-diol (PLA-diol) from lactic acid and 1,4-butanediol

2016 ◽  
Vol 19 (4) ◽  
pp. 58-65
Author(s):  
Ha Thi Thai La
Keyword(s):  
Molecular Weight ◽  
Lactic Acid ◽  
Polyethylene Glycol ◽  
1H Nmr ◽  
Biodegradable Polymer ◽  
Average Molecular Weight ◽  
Chain Extension ◽  
Structure And Properties ◽  
Chain Reaction ◽  
A Chain

In this research, the PLA-diol were synthesized from lactic acid (LA) and 1.4 butanediols (BD) with a tin octoate Sn(Oct)2 catalyst at a temperature of 180 °C and the pressure 5 mmHg. The structure and properties of PLA-diol are analyzed by the following methods: GPC, 1H-NMR and DSC. As a result, with the change in the content of Sn (Oct)2 from 0.1 to 1.0%, the molecular weight Mn of PLA - diol increased gradually from 4.119,2 to 7.359,6 g / mol . In addition, the BD content increased from 2.0% to 5.0%, the average molecular weight of the product decreased gradually from 7.536,9 g / mol to 4.735 g / mol, respectively. This change will affect the ability to use PLA-diol in the next denaturation research to apply in the field of biodegradable polymer such as copolymer with polyurethane, copolymer with polyethylene glycol diacid, or chain extension with other polymer in a chain reaction,...

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