scholarly journals Metatranscriptome analyses indicate resource partitioning between diatoms in the field

2015 ◽  
Vol 112 (17) ◽  
pp. E2182-E2190 ◽  
Author(s):  
Harriet Alexander ◽  
Bethany D. Jenkins ◽  
Tatiana A. Rynearson ◽  
Sonya T. Dyhrman
Keyword(s):  
Resource Utilization ◽  
Resource Partitioning ◽  
Niche Partitioning ◽  
The United States ◽  
Narragansett Bay ◽  
Marine Phytoplankton ◽  
Transcriptional Responses ◽  
Gene Sets ◽  
Nutrient Amendment

Diverse communities of marine phytoplankton carry out half of global primary production. The vast diversity of the phytoplankton has long perplexed ecologists because these organisms coexist in an isotropic environment while competing for the same basic resources (e.g., inorganic nutrients). Differential niche partitioning of resources is one hypothesis to explain this “paradox of the plankton,” but it is difficult to quantify and track variation in phytoplankton metabolism in situ. Here, we use quantitative metatranscriptome analyses to examine pathways of nitrogen (N) and phosphorus (P) metabolism in diatoms that cooccur regularly in an estuary on the east coast of the United States (Narragansett Bay). Expression of known N and P metabolic pathways varied between diatoms, indicating apparent differences in resource utilization capacity that may prevent direct competition. Nutrient amendment incubations skewed N/P ratios, elucidating nutrient-responsive patterns of expression and facilitating a quantitative comparison between diatoms. The resource-responsive (RR) gene sets deviated in composition from the metabolic profile of the organism, being enriched in genes associated with N and P metabolism. Expression of the RR gene set varied over time and differed significantly between diatoms, resulting in opposite transcriptional responses to the same environment. Apparent differences in metabolic capacity and the expression of that capacity in the environment suggest that diatom-specific resource partitioning was occurring in Narragansett Bay. This high-resolution approach highlights the molecular underpinnings of diatom resource utilization and how cooccurring diatoms adjust their cellular physiology to partition their niche space.

New approaches for Mars in-situ resource utilization based on the reverse water gas shift

1997 ◽  
Author(s):  
Robert Zubrin ◽  
Mitchell Clapp ◽  
Tom Meyer ◽  
Robert Zubrin ◽  
Mitchell Clapp ◽  
...  
Keyword(s):  
Resource Utilization ◽  
Water Gas Shift ◽  
New Approaches ◽  
Reverse Water Gas Shift ◽  
Water Gas

scholarly journals “Candidatus Dechloromonas phosphoritropha” and “Ca. D. phosphorivorans”, novel polyphosphate accumulating organisms abundant in wastewater treatment systems

2021 ◽  
Author(s):  
Francesca Petriglieri ◽  
Caitlin Singleton ◽  
Miriam Peces ◽  
Jette F. Petersen ◽  
Marta Nierychlo ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Niche Partitioning ◽  
16S Rrna Gene Sequencing ◽  
Abundant Species ◽  
Phosphate Removal ◽  
Rrna Gene ◽  
Biological Phosphorus Removal ◽  
Polyphosphate Accumulating Organisms

AbstractMembers of the genus Dechloromonas are often abundant in enhanced biological phosphorus removal (EBPR) systems and are recognized putative polyphosphate accumulating organisms (PAOs), but their role in phosphate removal is still unclear. Here, we used 16S rRNA gene sequencing and fluorescence in situ hybridization (FISH) to investigate the abundance and distribution of Dechloromonas spp. in Danish and global wastewater treatment plants. The two most abundant species worldwide revealed in situ dynamics of important intracellular storage polymers, measured by FISH-Raman in activated sludge from four full-scale EBPR plants and from a lab-scale reactor fed with different substrates. Moreover, seven distinct Dechloromonas species were determined from a set of ten high-quality metagenome-assembled genomes (MAGs) from Danish EBPR plants, each encoding the potential for polyphosphate (poly-P), glycogen, and polyhydroxyalkanoates (PHA) accumulation. The two species exhibited an in situ phenotype in complete accordance with the metabolic information retrieved by the MAGs, with dynamic levels of poly-P, glycogen, and PHA during feast-famine anaerobic–aerobic cycling, legitimately placing these microorganisms among the important PAOs. They are potentially involved in denitrification showing niche partitioning within the genus and with other important PAOs. As no isolates are available for the two species, we propose the names Candidatus Dechloromonas phosphoritropha and Candidatus Dechloromonas phosphorivorans.

scholarly journals Trends in medicare spending across strata of resource utilization among older individuals in the United States

2021 ◽  
Vol 36 ◽  
pp. 100873
Author(s):  
Christopher J.D. Wallis ◽  
Sabrina J. Poon ◽  
Pikki Lai ◽  
Liliana Podczerwinki ◽  
Melinda Beeuwkes Buntin
Keyword(s):  
United States ◽  
Resource Utilization ◽  
The United States ◽  
Older Individuals ◽  
Medicare Spending

scholarly journals Description and Derived Climatologies of Automated In Situ Eddy-Dissipation-Rate Reports of Atmospheric Turbulence

2014 ◽  
Vol 53 (6) ◽  
pp. 1416-1432 ◽  
Author(s):  
R. D. Sharman ◽  
L. B. Cornman ◽  
G. Meymaris ◽  
J. Pearson ◽  
T. Farrar
Keyword(s):  
Atmospheric Turbulence ◽  
Dissipation Rate ◽  
Route Planning ◽  
Federal Aviation Administration ◽  
The United States ◽  
Commercial Aircraft ◽  
Upper Troposphere ◽  
Obvious Benefit ◽  
Turbulence Data

AbstractThe statistical properties of turbulence at upper levels in the atmosphere [upper troposphere and lower stratosphere (UTLS)] are still not well known, partly because of the lack of adequate routine observations. This is despite the obvious benefit that such observations would have for alerting aircraft of potentially hazardous conditions, either in real time or for route planning. To address this deficiency, a research project sponsored by the Federal Aviation Administration has developed a software package that automatically estimates and reports atmospheric turbulence intensity levels (as EDR ≡ ε1/3, where ε is the energy or eddy dissipation rate). The package has been tested and evaluated on commercial aircraft. The amount of turbulence data gathered from these in situ reports is unprecedented. As of January 2014, there are ~200 aircraft outfitted with this system, contributing to over 137 million archived records of EDR values through 2013, most of which were taken at cruise levels of commercial aircraft, that is, in the UTLS. In this paper, techniques used for estimating EDR are outlined and comparisons with pilot reports from the same or nearby aircraft are presented. These reports allow calibration of EDR in terms of traditionally reported intensity categories (“light,” “moderate,” or “severe”). The results of some statistical analyses of EDR values are also presented. These analyses are restricted to the United States for now, but, as this program is expanded to international carriers, such data will begin to become available over other areas of the globe.

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