scholarly journals High-resolution in vivo imaging of mouse brain through the intact skull

2015 ◽  
Vol 112 (30) ◽  
pp. 9236-9241 ◽  
Author(s):  
Jung-Hoon Park ◽  
Wei Sun ◽  
Meng Cui
Keyword(s):  
High Resolution ◽  
Biological Tissue ◽  
Morphological Changes ◽  
Complex Refractive Index ◽  
Incident Light ◽  
Multiphoton Microscopy ◽  
Current Method ◽  
Tissue Imaging ◽  
Resolution Imaging

Multiphoton microscopy is the current method of choice for in vivo deep-tissue imaging. The long laser wavelength suffers less scattering, and the 3D-confined excitation permits the use of scattered signal light. However, the imaging depth is still limited because of the complex refractive index distribution of biological tissue, which scrambles the incident light and destroys the optical focus needed for high resolution imaging. Here, we demonstrate a wavefront-shaping scheme that allows clear imaging through extremely turbid biological tissue, such as the skull, over an extended corrected field of view (FOV). The complex wavefront correction is obtained and directly conjugated to the turbid layer in a noninvasive manner. Using this technique, we demonstrate in vivo submicron-resolution imaging of neural dendrites and microglia dynamics through the intact skulls of adult mice. This is the first observation, to our knowledge, of dynamic morphological changes of microglia through the intact skull, allowing truly noninvasive studies of microglial immune activities free from external perturbations.

scholarly journals Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism

2013 ◽  
Vol 200 (4) ◽  
pp. 407-418 ◽  
Author(s):  
Mini Jose ◽  
Sylvain Tollis ◽  
Deepak Nair ◽  
Jean-Baptiste Sibarita ◽  
Derek McCusker
Keyword(s):  
High Resolution ◽  
Membrane Trafficking ◽  
In Vivo Studies ◽  
Biological Processes ◽  
Constant Frequency ◽  
In Silico Modeling ◽  
Resolution Imaging ◽  
Polarity Establishment ◽  
Endocytic Vesicles

Formation of a stable polarity axis underlies numerous biological processes. Here, using high-resolution imaging and complementary mathematical modeling we find that cell polarity can be established via the spatial coordination of opposing membrane trafficking activities: endocytosis and exocytosis. During polarity establishment in budding yeast, these antagonistic processes become apposed. Endocytic vesicles corral a central exocytic zone, tightening it to a vertex that establishes the polarity axis for the ensuing cell cycle. Concomitantly, the endocytic system reaches an equilibrium where internalization events occur at a constant frequency. Endocytic mutants that failed to initiate periodic internalization events within the corral displayed wide, unstable polarity axes. These results, predicted by in silico modeling and verified by high resolution in vivo studies, identify a requirement for endocytic corralling during robust polarity establishment.

Zn-doping enhances the photoluminescence and stability of PbS quantum dots for in vivo high-resolution imaging in the NIR-II window

Nano Research ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 2239-2245 ◽  
Author(s):  
Xiulei Shi ◽  
Song Chen ◽  
Meng-Yao Luo ◽  
Biao Huang ◽  
Guozhen Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
High Resolution ◽  
High Resolution Imaging ◽  
Zn Doping ◽  
Resolution Imaging ◽  
Pbs Quantum Dots

scholarly journals Ultrabright red AIEgens for two-photon vascular imaging with high resolution and deep penetration

2018 ◽  
Vol 9 (10) ◽  
pp. 2705-2710 ◽  
Author(s):  
Wei Qin ◽  
Pengfei Zhang ◽  
Hui Li ◽  
Jacky W. Y. Lam ◽  
Yuanjing Cai ◽  
...  
Keyword(s):  
High Resolution ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
High Contrast ◽  
Deep Tissue ◽  
Two Photon ◽  
High Penetration ◽  
Deep Tissue Imaging

A successful strategy for the design of ultrabright red luminogens with aggregation-induced emission (AIE) features is reported. The AIE dots can be utilized as efficient fluorescent probes for in vivo deep-tissue imaging with high penetration depth and high contrast.

scholarly journals Targeted Molecular Imaging Using Aptamers in Cancer

2018 ◽  
Vol 11 (3) ◽  
pp. 71 ◽  
Author(s):  
Sorah Yoon ◽  
John Rossi
Keyword(s):  
Wide Spectrum ◽  
Rna Aptamers ◽  
Tissue Imaging ◽  
Live Cells ◽  
Target Cells ◽  
New Class ◽  
Non Invasive ◽  
Resolution Imaging ◽  
Tissue Specimens

Imaging is not only seeing, but also believing. For targeted imaging modalities, nucleic acid aptamers have features such as superior recognition of structural epitopes and quick uptake in target cells. This explains the emergence of an evolved new class of aptamers into a wide spectrum of imaging applications over the last decade. Genetically encoded biosensors tagged with fluorescent RNA aptamers have been developed as intracellular imaging tools to understand cellular signaling and physiology in live cells. Cancer-specific aptamers labeled with fluorescence have been used for assessment of clinical tissue specimens. Aptamers conjugated with gold nanoparticles have been employed to develop innovative mass spectrometry tissue imaging. Also, use of chemically conjugated cancer-specific aptamers as probes for non-invasive and high-resolution imaging has been transformative for in vivo imaging in multiple cancers.

scholarly journals Hemoglobin Video Imaging Provides Novel In Vivo High-Resolution Imaging and Quantification of Human Aqueous Outflow in Patients with Glaucoma

2019 ◽  
Vol 2 (5) ◽  
pp. 327-335 ◽  
Author(s):  
Tasneem Z. Khatib ◽  
Paul A.R. Meyer ◽  
Jed Lusthaus ◽  
Ilya Manyakin ◽  
Yusuf Mushtaq ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Video Imaging ◽  
Resolution Imaging ◽  
Aqueous Outflow

scholarly journals Method for high-resolution imaging of creatine in vivo using chemical exchange saturation transfer

2013 ◽  
Vol 71 (1) ◽  
pp. 164-172 ◽  
Author(s):  
Feliks Kogan ◽  
Mohammad Haris ◽  
Anup Singh ◽  
Kejia Cai ◽  
Catherine Debrosse ◽  
...  
Keyword(s):  
High Resolution ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
High Resolution Imaging ◽  
Saturation Transfer ◽  
Resolution Imaging

High-Resolution Imaging of the Human Retina In Vivo after Scatter Photocoagulation Treatment Using a Semiautomated Laser System

Ophthalmology ◽  
2010 ◽  
Vol 117 (3) ◽  
pp. 545-551 ◽  
Author(s):  
Katharina Kriechbaum ◽  
Matthias Bolz ◽  
Gabor G. Deak ◽  
Sonja Prager ◽  
Christoph Scholda ◽  
...  
Keyword(s):  
High Resolution ◽  
Laser System ◽  
High Resolution Imaging ◽  
Human Retina ◽  
Photocoagulation Treatment ◽  
Resolution Imaging

Multiparametric high-resolution imaging of barley embryos by multiphoton microscopy and magnetic resonance micro-imaging

2007 ◽  
Vol 70 (5) ◽  
pp. 426-432 ◽  
Author(s):  
Martin Stark ◽  
Bertram Manz ◽  
Alexander Ehlers ◽  
Markus Küppers ◽  
Iris Riemann ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
High Resolution ◽  
Multiphoton Microscopy ◽  
High Resolution Imaging ◽  
Resolution Imaging

scholarly journals Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2312-2317 ◽  
Author(s):  
Fanghao Hu ◽  
Lu Wei ◽  
Chaogu Zheng ◽  
Yihui Shen ◽  
Wei Min
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Mammalian Cells ◽  
Stimulated Raman Scattering ◽  
Primary Neurons ◽  
High Resolution Imaging ◽  
Metabolic Labeling ◽  
C Elegans ◽  
Resolution Imaging

High-resolution imaging of choline metabolites in living mammalian cells, primary neurons andC. eleganshas been demonstrated with the potential forin vivodisease detection and developmental monitoring.

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