scholarly journals Leveraging abscisic acid receptors for efficient water use in Arabidopsis

2016 ◽  
Vol 113 (24) ◽  
pp. 6791-6796 ◽  
Author(s):  
Zhenyu Yang ◽  
Jinghui Liu ◽  
Stefanie V. Tischer ◽  
Alexander Christmann ◽  
Wilhelm Windisch ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Plant Growth ◽  
Water Deficit ◽  
Water Use ◽  
Plant Biomass ◽  
Water Productivity ◽  
Carbon Assimilation ◽  
High Growth ◽  
Trade Offs ◽  
Aba Receptors

Plant growth requires the influx of atmospheric CO2 through stomatal pores, and this carbon uptake for photosynthesis is inherently associated with a large efflux of water vapor. Under water deficit, plants reduce transpiration and are able to improve carbon for water exchange leading to higher water use efficiency (WUE). Whether increased WUE can be achieved without trade-offs in plant growth is debated. The signals mediating the WUE response under water deficit are not fully elucidated but involve the phytohormone abscisic acid (ABA). ABA is perceived by a family of related receptors known to mediate acclimation responses and to reduce transpiration. We now show that enhanced stimulation of ABA signaling via distinct ABA receptors can result in plants constitutively growing at high WUE in the model species Arabidopsis. WUE was assessed by three independent approaches involving gravimetric analyses, 13C discrimination studies of shoots and derived cellulose fractions, and by gas exchange measurements of whole plants and individual leaves. Plants expressing the ABA receptors RCAR6/PYL12 combined up to 40% increased WUE with high growth rates, i.e., are water productive. Water productivity was associated with maintenance of net carbon assimilation by compensatory increases of leaf CO2 gradients, thereby sustaining biomass acquisition. Leaf surface temperatures and growth potentials of plants growing under well-watered conditions were found to be reliable indicators for water productivity. The study shows that ABA receptors can be explored to generate more plant biomass per water transpired, which is a prime goal for a more sustainable water use in agriculture.

scholarly journals Increased water use efficiency and water productivity of arabidopsis by abscisic acid receptors from Populus canescens

2019 ◽  
Vol 124 (4) ◽  
pp. 581-589 ◽  
Author(s):  
Michael Papacek ◽  
Alexander Christmann ◽  
Erwin Grill
Keyword(s):  
Abscisic Acid ◽  
Water Use Efficiency ◽  
Water Use ◽  
Water Productivity ◽  
Trade Offs ◽  
Enhanced Expression ◽  
Physiological Analysis ◽  
Specific Receptors ◽  
Use Efficiency ◽  
Aba Receptors

Abstract Background and Aims Water deficit is the single most important factor limiting plant productivity in the field. Poplar is a crop used for second-generation bioenergy production that can be cultivated on marginal land without competing for land use in food production. Poplar has a high demand for water, which makes improving its water use efficiency (WUE) an attractive goal. Recently, we showed that enhanced expression of specific receptors of arabidopsis for the phytohormone abscisic acid (ABA) can improve WUE in arabidopsis and water productivity, i.e. more biomass is formed per unit of water over time. In this study, we examined whether ABA receptors from poplar can enhance WUE and water productivity in arabidopsis. Methods ABA receptors from poplar were stably introduced into arabidopsis for analysis of their effect on water use efficiency. Physiological analysis included growth assessment and gas exchange measurements. Key Results The data presented here are in agreement with the functionality of poplar ABA receptors in arabidopsis, which led to ABA-hypersensitive seed germination and root growth. In addition, arabidopsis lines expressing poplar RCAR10, but not RCAR9, showed increased WUE by up to 26 % compared with the wild type with few trade-offs in growth that also resulted in higher water productivity during drought. The improved WUE was mediated by reduced stomatal conductance, a steeper CO2 gradient at the leaf boundary and sustained photosynthesis resulting in an increased intrinsic WUE (iWUE). Conclusions The analysis is a case study supporting the use of poplar ABA receptors for improving WUE and showing the feasibility of using a heterologous expression strategy for generating plants with improved water productivity.

A quantitative top-down view of interactions between stresses: theory and analysis of nitrogen - water co-limitation in Mediterranean agro-ecosystems

2005 ◽  
Vol 56 (11) ◽  
pp. 1151 ◽  
Author(s):  
Victor O. Sadras
Keyword(s):  
Plant Growth ◽  
Grain Yield ◽  
Water Use Efficiency ◽  
Water Use ◽  
Nitrogen Availability ◽  
Growth And Yield ◽  
Top Down ◽  
Trade Offs ◽  
Eastern Australia ◽  
Use Efficiency

The multiple factors constraining the growth, reproduction, and survival of diverse organisms are often non-additive. Research of interacting factors generally involves conceptual models that are specific for target organism, type of stress, and process. As a complement to this reductionist, bottom-up view, in this review I discuss a quantitative top-down approach to interacting stresses based on co-limitation theory. Firstly, co-limitation theory is revised. Co-limitation is operationally identified when the output response of a biological system (e.g. plant or population growth) to two or more inputs is greater than its response to each factor in isolation. The hypothesis of Bloom, Chapin, and Mooney, that plant growth is maximised when it is equally limited by all resources, is reworded in terms of co-limitation and formulated in quantitative terms, i.e. for a given intensity of aggregate stress, plant growth is proportional to degree of resource co-limitation. Emphasis is placed on the problems associated with the quantification of co-limitation. It is proposed that seasonal indices of nitrogen and water stress calculated with crop simulation models can be integrated in indices accounting for the aggregated intensity of water and nitrogen stress (SWN), the degree of water and nitrogen co-limitation (CWN), and the integrated effect of stress and co-limitation (SCWN = CWN/SWN). The expectation is that plant growth and yield should be an inverse function of stress intensity and a direct function of co-limitation, thus proportional to SCWN. Secondly, the constraints imposed by water and nitrogen availability on yield and water use efficiency of wheat crops are highlighted in case studies of low-input farming systems of south-eastern Australia. Thirdly, the concept of co-limitation is applied to the analysis of (i) grain yield responses to water–nitrogen interactions, and (ii) trade-offs between nitrogen- and water-use efficiency. In agreement with theoretical expectations, measured grain yield is found to be proportional to modelled SCWN. Productivity gains associated with intensification of cropping practices are interpreted in terms of a trade-off, whereby water-use efficiency is improved at the expense of nitrogen-use efficiency, thus leading to a higher degree of resource co-limitation.

scholarly journals Simultaneous stimulation of RuBP regeneration and electron transport increases productivity and water use efficiency under field conditions

2020 ◽  
Author(s):  
Patricia E. López-Calcagno ◽  
Kenny L. Brown ◽  
Andrew J. Simkin ◽  
Stuart J. Fisk ◽  
Tracy Lawson ◽  
...  
Keyword(s):  
Electron Transport ◽  
Water Use Efficiency ◽  
Water Use ◽  
Plant Biomass ◽  
Carbon Assimilation ◽  
Field Conditions ◽  
Simultaneous Stimulation ◽  
Intrinsic Water Use Efficiency ◽  
Use Efficiency ◽  
Stimulation Of

AbstractPrevious studies have demonstrated that independent stimulation of either electron transport or RuBP regeneration can increase the rate of photosynthetic carbon assimilation and plant biomass. In this paper, we present evidence that a multi-gene approach to simultaneously manipulate these two processes provides a further stimulation of photosynthesis. We report on the introduction of the cyanobacterial bifunctional enzyme fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase or overexpression of the plant enzyme sedoheptulose-1,7-bisphosphatase, together with expression of the red algal protein cytochrome c6, and show that a further increase in biomass accumulation under both glasshouse and field conditions can be achieved. Furthermore, we provide evidence that the simultaneous stimulation of electron transport and RuBP regeneration can lead to enhanced intrinsic water use efficiency under field conditions.One sentence summarySimultaneous stimulation of RuBP regeneration and electron transport results in improvements in biomass yield in glasshouse and field grown tobacco.

scholarly journals Abscisic Acid Receptors and Coreceptors Modulate Plant Water Use Efficiency and Water Productivity

2019 ◽  
Vol 180 (2) ◽  
pp. 1066-1080 ◽  
Author(s):  
Zhenyu Yang ◽  
Jinghui Liu ◽  
Fabien Poree ◽  
Rudi Schaeufele ◽  
Hendrik Helmke ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Water Use Efficiency ◽  
Water Use ◽  
Water Productivity ◽  
Plant Water ◽  
Plant Water Use ◽  
Use Efficiency

scholarly journals Salinity Tolerance and Leaf Water Use Efficiency in Citrus

2010 ◽  
Vol 135 (1) ◽  
pp. 33-39 ◽  
Author(s):  
James P. Syvertsen ◽  
Juan C. Melgar ◽  
Francisco García-Sánchez
Keyword(s):  
Plant Growth ◽  
Water Use Efficiency ◽  
Water Use ◽  
Salinity Tolerance ◽  
Weight Ratio ◽  
High Growth ◽  
Poncirus Trifoliata ◽  
Published Data ◽  
Citrus Rootstock ◽  
Use Efficiency

In three separate experiments, the growth and water use of salinized citrus rootstock seedlings and grafted trees were modified using different growth substrates, elevated CO2, or 50% shade screen under field conditions. By reanalyzing previously published data, we tested the hypothesis that salinity tolerance in citrus can be characterized as the ability to maintain low levels of leaf Cl− accumulation through high plant growth and high water use efficiency (WUE) under saline conditions. Well-irrigated salinized seedlings of the relatively salt-sensitive Carrizo citrange [Carr (Citrus sinensis × Poncirus trifoliata)] were grown in sand, clay, or a peat-based soilless media. Salinity stress reduced plant growth and water use. Leaf Cl− concentration was negatively related to plant growth, but leaf Cl− increased with transpiration rate in low-saline treatments. In a second experiment using salinized seedlings of the relatively salt-tolerant Cleopatra mandarin [Cleo (Citrus reticulata)] grown along with Carr seedlings with or without elevated CO2, leaf Cl− was negatively related to growth and to shoot/root dry weight ratio, but was positively related to water use such that leaf Cl− was negatively related to leaf WUE. In a third experiment using salinized 2-year-old ‘Valencia’ orange (C. sinensis) trees grafted on Cleo or Carr rootstocks and grown with or without shadecloth, leaf Cl− was positively related to leaf transpiration as both were higher in the spring than in the fall, regardless of rootstock or shade treatment. Overall, leaf Cl− was positively related to water use and was negatively related to leaf WUE. High growth, low water use, and consequently, high WUE of salinized citrus were related to low leaf Cl−. Such relationships can be used as indicators of salinity tolerance.

scholarly journals Abscisic Acid Receptors Modulate Metabolite Levels and Phenotype in Arabidopsis Under Normal Growing Conditions

Metabolites ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 249 ◽  
Author(s):  
Xiaoyi Li ◽  
Lintao Wu ◽  
Yao Qiu ◽  
Tao Wang ◽  
Qin Zhou ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Plant Growth ◽  
Aba Signaling ◽  
Primary Metabolite ◽  
Abnormal Growth ◽  
Phenotype Analysis ◽  
Positive Regulators ◽  
Aba Receptor ◽  
Growing Conditions ◽  
Aba Receptors

Abscisic acid (ABA) is a vital phytohormone that accumulates in response to various biotic and abiotic stresses, as well as plant growth. In Arabidopsis thaliana, there are 14 members of the ABA receptor family, which are key positive regulators involved in ABA signaling. Besides reduced drought stress tolerance, the quadruple and sextuple mutants (pyr1pyl1pyl2pyl4 (1124) and pyr1pyl1pyl2pyl4pyl5pyl8 (112458) show abnormal growth phenotypes, such as decreases in yield and height, under non-stress conditions. However, it remains unknown whether ABA receptors mediate ABA signaling to regulate plant growth and development. Here, we showed the primary metabolite profiles of 1124, 112458 and wild-type (WT) plants grown under normal conditions. The metabolic changes were significantly different between ABA receptor mutants and WT. Guanosine, for the biosynthesis of cyclic guanosine 3′,5′-monophosphate (cGMP), is an important second messenger that acts to regulate the level of ABA. In addition, other amino acids were increased in the 112458 mutant, including proline. These results, together with phenotype analysis, indicated that ABA receptors are involved in ABA signaling to modulate metabolism and plant growth under normal conditions.

scholarly journals Water deficit effect on the accumulation of biomass and artemisinin in annual wormwood(Artemisia annua L., Asteraceae)

2010 ◽  
Vol 22 (1) ◽  
pp. 1-9 ◽  
Author(s):  
José A. Marchese ◽  
Jorge F.S. Ferreira ◽  
Vera L.G. Rehder ◽  
Osmar Rodrigues
Keyword(s):  
Plant Growth ◽  
Water Deficit ◽  
Plant Biomass ◽  
Artemisia Annua ◽  
Turgor Pressure ◽  
Chemical System ◽  
Limiting Factor ◽  
Biotic And Abiotic Stress ◽  
Artemisia Annua L ◽  
Severe Water Deficit

Despite the importance of Artemisia annua L. as the only source of the anti-parasitic drug artemisinin, little is known on the effects of biotic and abiotic stress on artemisinin accumulation. Water deficit is the most limiting factor on plant growth, however it can trigger secondary metabolite accumulation, depending on the plant growth stage and intensity. A. annua cultivated in growth chambers was submitted to five water deficit treatments (watered, 14, 38, 62 e 86 hours without irrigation). Water deficits of 38 and 62 hours (Yw = -1.39 and -2.51 MPa, respectively) increased leaf artemisinin content, but only 38 hours led to a significant increase in both leaf and plant artemisinin (29%), with no detriment to plant biomass production. The other treatments had no effect on, or decreased artemisinin accumulation. A. annua plants tolerated well water deficit treatments, including the most severe water deficit applied (Yw -3.97 MPa or 86 hs without irrigation) and recovered their turgor pressure after rehydration. These results suggest that moderate water deficit prior to harvesting the crop may not only reduce time and costs in drying the crop, but can also induce artemisinin accumulation, both of which increase crop profit margins. Results also suggest that artemisinin could be part of A. annua chemical system of defense against water deficit.

scholarly journals Metal Accumulation Profile of Catharanthus roseus (L.) G.Don and Celosia argentea L. with EDTA Co-Application

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 598
Author(s):  
Muneeba Qurban ◽  
Cyrus Raza Mirza ◽  
Aqib Hassan Ali Khan ◽  
Walid Khalifa ◽  
Mustapha Boukendakdji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Catharanthus Roseus ◽  
Metal Uptake ◽  
Metal Accumulation ◽  
Metal Tolerance ◽  
Plant Biomass ◽  
Ethylenediaminetetraacetic Acid ◽  
Ornamental Plants ◽  
Environmental Deterioration ◽  
Celosia Argentea

The problem of metal-induced toxicity is proliferating with an increase in industrialization and urbanization. The buildup of metals results in severe environmental deterioration and harmful impacts on plant growth. In this study, we investigated the potential of two ornamental plants, Catharanthus roseus (L.) G.Don and Celosia argentea L., to tolerate and accumulate Ni, Cr, Cd, Pb, and Cu. These ornamental plants were grown in Hoagland’s nutrient solution containing metal loads (50 µM and 100 µM) alone and in combination with a synthetic chelator, ethylenediaminetetraacetic acid (EDTA) (2.5 mM). Plant growth and metal tolerance varied in both plant species for Ni, Cr, Cd, Pb, and Cu. C. roseus growth was better in treatments without EDTA, particularly in Ni, Cr, and Pb treatments, and Pb content increased in all parts of the plant. In contrast, Cd content decreased with EDTA addition. In C. argentea, the addition of EDTA resulted in improved plant biomass at both doses of Cu. In contrast, plant biomass reduced significantly in the case of Ni. In C. argentea, without EDTA, root length in Cd and Cu treatments was significantly lower than the control and other treatments. However, the addition of EDTA resulted in improved growth at both doses for Pb and Cu. Metal accumulation in C. argentea enhanced significantly with EDTA addition at both doses of Cu and Cd. Hence, it can be concluded that EDTA addition resulted in improved growth and better metal uptake than treatments without EDTA. Metal accumulation increased with EDTA addition compared to treatments without EDTA, particularly for Pb in C. roseus and Cu and Cd in C. argentea. Based on the present results, C. roseus showed a better ability to phytostabilize Cu, Cd, and Ni, while C. argentea worked better for Ni, Cd, Cu, and Pb.

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