scholarly journals The Influence of Neighboring Base Pairs upon Base-Pair Substitution Mutation Rates

1971 ◽  
Vol 68 (4) ◽  
pp. 773-776 ◽  
Author(s):  
R. E. Koch
Keyword(s):  
Base Pair ◽  
Mutation Rates ◽  
Base Pairs ◽  
Base Pair Substitution ◽  
Substitution Mutation

scholarly journals Induction of Micronuclei, Base-pair Substitution Mutation and Excision-repair Deficient by Polluted Water from Asa River in Nigeria

2019 ◽  
Vol 4 (2) ◽  
pp. 68-77
Author(s):  
Anifowoshe T Abass ◽  
Oladipo S Olayinka ◽  
Adebayo O Mutolib ◽  
Eboh O Solomon ◽  
Abdussalam A Rasheedat ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Base Pair ◽  
Water Samples ◽  
Ames Test ◽  
Excision Repair ◽  
Clarias Gariepinus ◽  
Polluted Water ◽  
Base Pair Substitution ◽  
Peripheral Erythrocyte ◽  
Substitution Mutation

AbstractAsa river is a major river designated to supply millions of people of Ilorin, Kwara State, Nigeria potable water for drinking but its managements is of grave concern due to anthropogenic activities. Thus, evaluation of genotoxicity of this river was carried out by subjecting the water samples and fish therein to three bioassays (Micronucleus (MN) assay, Ames test and SOS-chromo test). Physicochemical parameters and heavy metals were analysed at three different stations (Aliara (SI), Unity (SII) and Tuyil (SIII)) of the river. In SII, most of the heavy metals analysed were above the acceptable limits compare to SI and SIII. The peripheral erythrocyte of the fishes (Oreochromis niloticus, Synodontis batensoda, Synodontis eupterus, Clarias gariepinus and Clarias angullaris) at SI and SII stations showed a significant (p<0.05) induction of MN and different nuclear abnormalities (NA). Water samples from the three stations subjected to Ames test (Salmonella typhimurium TA100) and SOS chromotests (Escherichia coli PQ37) at 25%, 50% and 100% concentrations showed statistically significant (p<0.05) induction of DNA damage at all concentrations in the two tester strains, thus indicating base-pair substitution mutation and excision-repairdeficient, respectively, by the water samples. Therefore, drinking of this water and/or consumption of fish from this river should be taken with caution to avoid a carcinogenic risk.

scholarly journals MISREPAIR MUTAGENESIS IN BACTERIOPHAGE T4

Genetics ◽  
1974 ◽  
Vol 78 (1) ◽  
pp. 81-89
Author(s):  
Ronald R Green ◽  
John W Drake
Keyword(s):  
Uv Irradiation ◽  
White Light ◽  
Base Pair ◽  
Bacteriophage T4 ◽  
Spontaneous Mutation ◽  
Light Irradiation ◽  
Mutation Rates ◽  
Repair System ◽  
Base Analog ◽  
Substitution Mutation

ABSTRACT The T4 mutations px, y and 1206 inactivate an error-prone recombination-like repair system, reducing or abolishing mutagenesis by UV irradiation, MMS, and white light irradiation in the presence of the photosensitizer 8MOP. Both px and y increase some spontaneous mutation rates and slightly enhance proflavin mutagenesis; neither mutation affects thymineless or 2AP mutagenesis appreciably, but both mildly enhance 5BU mutagenesis. The mutation hm promotes UV, MMS, photodynamic, thymineless, and base analog mutagenesis, in addition to spontaneous base pair substitution mutation. It does not, however, markedly affect proflavin mutagenesis. The px mutation maps in the vicinity of genes 41-56, and the hm mutation maps in the vicinity of genes rI-v.

Rates of Spontaneous Mutation

Genetics ◽  
1998 ◽  
Vol 148 (4) ◽  
pp. 1667-1686 ◽  
Author(s):  
John W Drake ◽  
Brian Charlesworth ◽  
Deborah Charlesworth ◽  
James F Crow
Keyword(s):  
Cell Division ◽  
Base Pair ◽  
Rna Viruses ◽  
Spontaneous Mutation ◽  
Mutation Rates ◽  
Base Pairs ◽  
Evolutionary Forces ◽  
Sexual Generation ◽  
Higher Eukaryotes

Abstract Rates of spontaneous mutation per genome as measured in the laboratory are remarkably similar within broad groups of organisms but differ strikingly among groups. Mutation rates in RNA viruses, whose genomes contain ca. 104 bases, are roughly 1 per genome per replication for lytic viruses and roughly 0.1 per genome per replication for retroviruses and a retrotransposon. Mutation rates in microbes with DNA-based chromosomes are close to 1/300 per genome per replication; in this group, therefore, rates per base pair vary inversely and hugely as genome sizes vary from 6 × 103 to 4 × 107 bases or base pairs. Mutation rates in higher eukaryotes are roughly 0.1–100 per genome per sexual generation but are currently indistinguishable from 1/300 per cell division per effective genome (which excludes the fraction of the genome in which most mutations are neutral). It is now possible to specify some of the evolutionary forces that shape these diverse mutation rates.

Mutagenicity of Bisbenzimidazole Derivatives

2010 ◽  
Vol 65 (1-2) ◽  
pp. 10-14 ◽  
Author(s):  
Filiz Susuz Alanyalı ◽  
Merve Arıcı ◽  
Öge Artagan ◽  
İlhan Işıkdağ ◽  
Yusuf Özkay
Keyword(s):  
Salmonella Typhimurium ◽  
Base Pair ◽  
Frameshift Mutation ◽  
Metabolic Activation ◽  
Mutagenic Response ◽  
Base Pair Substitution ◽  
Incorporation Assay ◽  
Substitution Mutation

The mutagenicities of 2,2’-(di-3-hydroxyphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-(di- 4-hy droxyphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-(di-3-methoxyphenyl)-1H,1H’-[5,5’]- bisbenzimidazole, 2,2’-bis-(4-nitrophenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-bis-(3-nitrophenyl)- 1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-bis-(4-methylphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, 2,2’-(di-4-methoxyphenyl)-1H,1H’-[5,5’]-bisbenzimidazole, and 2,2’-bis-(3-me thylphenyl)- 1H,1H’-[5,5’]-bisbenzimidazole were studied in vitro using two strains of Salmonella typhimurium with frameshift mutation (TA98) and base-pair substitution mutation (TA100) as the plate incorporation assay in the absence of metabolic activation. These compounds are currently used to treat cancer. 4-Nitrophenyl and 3-nitrophenyl compounds were found to be mutagenic on both strains of Salmonella. A clear mutagenic response was seen in nitro-bound derivatives. The mutagenic response in Salmonella test strains (TA98, TA100) and structures of molecules suggest that nitro-bound molecules could be mutagenic.

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

Micropollutants Produced by Disinfection of Wastewater Effluents*

1982 ◽  
Vol 14 (12) ◽  
pp. 45-59 ◽  
Author(s):  
R L Jolley ◽  
R B Cumming ◽  
N E Lee ◽  
J E Thompson ◽  
L R Lewis
Keyword(s):  
Wastewater Treatment ◽  
Uv Irradiation ◽  
Base Pair ◽  
Wastewater Treatment Plants ◽  
Department Of Energy ◽  
Union Carbide Corporation ◽  
Organic Constituents ◽  
Chemical Effects ◽  
Base Pair Substitution ◽  
The U.S

The principal objective of this research program was to examine the effects of disinfection by chlorine, ozone, and ultraviolet light (uv) irradiation on nonvolatile organic constituents relative to chemical effects and the formation of micropollutants. In a comparative study of highly concentrated samples of effluents from nine wastewater treatment plants, it was determined that disinfection with chlorine or ozone both destroys and produces nonvolatile organic constituents including mutagenic constituents. The chemical effects of disinfection by uv irradiation were relatively slight, although the mutagenic constituents in one effluent were eliminated by this treatment. The nine wastewater treatment plants were selected by using the following criteria: disinfection method, nature of wastewater source, type of wastewater treatment, standards for quality of treatment, and geographical location. The treatment plants varied from pilot plant and small plants [0.05 m3/s (1 Mgd)] treating principally domestic waste to large plants [4.4 m3/s (100 Mgd)] treating principally industrial waste. Four plants used only chlorine for disinfection, four used ozone for disinfection, and one used uv irradiation for disinfection. Eight treatment plants used conventional secondary or more advanced wastewater treatment, and one plant used primary treatment. The following methodology was used in this investigation: grab sample collection of 40-L samples of undisinfected and disinfected effluents; concentration of the effluents by lyophilization; high-pressure liquid chromatographic separation of nonvolatile organic constituents in effluent concentrates using uv absorbance, cerate oxidation, and fluorescence detectors; bacterial mutagenicity testing of concentrates and chromatographic fractions; and identification and characterization of nonvolatile organic constituents in mutagenic HPLC fractions. With these procedures, over 100 micropollutants were identified in the wastewater effluent concentrates. Interplant comparison revealed considerable variability in the presence of mutagenic nonvolatile organic constituents in the undisinfected effluent concentrates as well as much variability in the destruction of the mutagenic constituents and the formation of other mutagenic constituents as a result of disinfection. Moreover, the effects varied on samples collected at the same wastewater treatment plant at different periods. No micropollutants known to be mutagens were identified in the mutagenic HPLC fractions separated from the undisinfected, chlorinated, and ozonated effluent concentrates. The mutagenic activity of the nonvolatile organic constituents in one chlorinated effluent concentrate was not attributable to organic chloramines. Most of the mutagens detected in effluent concentrates are direct acting and do not require metabolic activation. Both base-pair substitution mutagens and frame-shift mutagens occurred in the wastewater concentrates, but the former type was more frequent. For many of the compounds in effluents, strain TA-1535 was more sensitive than strain TA-100 in detecting base-pair substitution mutagens. *Research sponsored by the U.S. Department of Energy and the U.S. Environmental Protection Agency. The work was carried out at the Oak Ridge National Laboratory, which is operated by the U.S. Department of Energy under contract W-7405-eng-26 with the Union Carbide Corporation.

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