scholarly journals Recircularization and Autonomous Replication of a Sheared R-Factor DNA Segment in Escherichia coli Transformants

1973 ◽  
Vol 70 (5) ◽  
pp. 1293-1297 ◽  
Author(s):  
S. N. Cohen ◽  
A. C. Y. Chang
Keyword(s):  
Escherichia Coli ◽  
Autonomous Replication ◽  
R Factor

scholarly journals Recombination and complementation between R factors inEscherichia coliK 12

1971 ◽  
Vol 18 (3) ◽  
pp. 287-297 ◽  
Author(s):  
T. J. Foster ◽  
T. G. B. Howe
Keyword(s):  
Escherichia Coli ◽  
Specific Activity ◽  
Chloramphenicol Acetyl Transferase ◽  
Wild Type ◽  
Chloramphenicol Resistance ◽  
Low Level ◽  
R Factor ◽  
Reduced Frequency ◽  
Interallelic Complementation ◽  
R Factors

SUMMARYRecombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated inEscherichia coliK12rec+; it occurs at reduced frequency inrecBandrecC, and is not detectable inreeA, indicating that R factor recombination depends on host functions. Some mutants of R1 also recombine with an R100 mutant in a similar way.recAcells carrying an R1 and an R100 Cmsmutant (hetero-R state) have a low level of chloramphenicol-resistance, and form a chloramphenicol acetyl transferase that has lower specific activity than enzyme from hosts carrying wild-type or recombinant factors. These results suggest the occurrence of interallelic complementation between mutant R factors.

scholarly journals Characterization of the β-lactamase specified by the resistance factor R-1818 in Escherichia coli K12 and other Gram-negative bacteria

1971 ◽  
Vol 123 (4) ◽  
pp. 501-505 ◽  
Author(s):  
J. W. Dale
Keyword(s):  
Escherichia Coli ◽  
Host Species ◽  
Host Bacterium ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
R Factor ◽  
Relationship Of ◽  
The Relationship

1. The amino acid composition of the β-lactamase from E. coli (R-1818) was determined. 2. The R-1818 β-lactamase is inhibited by formaldehyde, hydroxylamine, sodium azide, iodoacetamide, iodine and sodium chloride. 3. The Km values for benzylpenicillin, ampicillin and oxacillin have been determined by using the R-factor enzyme from different host species. The same values were obtained, irrespective of the host bacterium. 4. The molecular weight of the enzyme was found to be 44600, and was the same for all host species. 5. The relationship of R-1818 and R-GN238 β-lactamases is discussed.

R-factor-mediated suppression of the galactose-sensitive phenotype of Escherichia coli K-12 galE mutants

Plasmid ◽  
1981 ◽  
Vol 6 (1) ◽  
pp. 78-85
Author(s):  
Anna Fietta ◽  
Guido Grandi ◽  
Massimo Malcovati ◽  
Gianna Valentini ◽  
Vittorio Sgaramella ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
R Factor ◽  
K 12

scholarly journals ISOLATION AND CHARACTERIZATION OF LIVIDOMYCIN A INACTIVATED BY PSEUDOMONAS AERUGINOSA AND ESCHERICHIA COLI CARRYING R FACTOR

1972 ◽  
Vol 25 (8) ◽  
pp. 483-484 ◽  
Author(s):  
SHINICHI KONDO ◽  
HARUO YAMAMOTO ◽  
HIROSHI NAGANAWA ◽  
HAMAO UMEZAWA ◽  
SUSUMU MITSUHASHI
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
R Factor ◽  
Isolation And Characterization

scholarly journals Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310

1972 ◽  
Vol 130 (1) ◽  
pp. 55-62 ◽  
Author(s):  
J. Melling ◽  
G. K. Scott
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Enzyme Activity ◽  
Coli Strain ◽  
Specific Enzyme ◽  
Specific Enzyme Activity ◽  
E Coli ◽  
R Factor

Purified penicillinase, in gram quantities, has been prepared from Escherichia coli strain W3310 by using methods developed to handle large amounts of material. The final product had a specific enzyme activity of 3.08 units/μg of protein, which was over twice as high as that reported previously (Datta & Richmond, 1966). The purified enzyme was similar to that from E. coli strain TEM, but different in molecular weight and some other respects. The differences observed may be a result of the greater purity obtained.

scholarly journals R Factor-mediated Resistance to Ultraviolet Light in Strains of Escherichia coli Deficient in Known Repair Functions

1976 ◽  
Vol 93 (1) ◽  
pp. 103-110 ◽  
Author(s):  
D. J. Tweats ◽  
M. J. Thompson ◽  
R. J. Pinney ◽  
J. T. Smith
Keyword(s):  
Escherichia Coli ◽  
Ultraviolet Light ◽  
R Factor
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