scholarly journals Localization of low density lipoprotein receptors on plasma membrane of normal human fibroblasts and their absence in cells from a familial hypercholesterolemia homozygote.

1976 ◽  
Vol 73 (7) ◽  
pp. 2434-2438 ◽  
Author(s):  
R. G. Anderson ◽  
J. L. Goldstein ◽  
M. S. Brown
Keyword(s):  
Plasma Membrane ◽  
Familial Hypercholesterolemia ◽  
Low Density Lipoprotein ◽  
Human Fibroblasts ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptors ◽  
Normal Human ◽  
In Cells

scholarly journals Effect of cell density on binding and uptake of low density lipoprotein by human fibroblasts.

1979 ◽  
Vol 83 (3) ◽  
pp. 588-594 ◽  
Author(s):  
H S Kruth ◽  
J Avigan ◽  
W Gamble ◽  
M Vaughan
Keyword(s):  
Cell Density ◽  
Low Density Lipoprotein ◽  
Human Fibroblasts ◽  
Density Lipoprotein ◽  
Low Density ◽  
Cellular Lipid ◽  
Cholesterol Accumulation ◽  
Ldl Receptors ◽  
Ldl Binding ◽  
In Cells

The effect of cell density on low density lipoprotein (LDL) binding by cultured human skin fibroblasts was investigated. Bound LDL was visualized by indirect immunofluorescence. Cellular lipid and cholesterol were monitored by fluorescence in cells stained with phosphine 3R and filipin, respectively. LDL binding and lipid accumulation were compared in cells in stationary and exponentially growing cultures, in sparsely and densely plated cultures, in wounded and non-wounded areas of stationary cultures, and in stationary cultures with and without the addition of lipoprotein-deficient serum. We conclude that LDL binding and cholesterol accumulation induced by LDL are influenced by cell density. It appears that, compared to rapidly growing cells, quiescent (noncycling) human fibroblasts exhibit fewer functional LDL receptors.

Release of low density lipoprotein receptors from human fibroblasts or Hela cells by tryptic digestion

1978 ◽  
Vol 84 (2) ◽  
pp. 366-373 ◽  
Author(s):  
Nicola Di Ferrante ◽  
Patricia V. Donnelly ◽  
Daniela T. Di Ferrante ◽  
Salvatore Toma ◽  
Antonio M. Gotto
Keyword(s):  
Hela Cells ◽  
Low Density Lipoprotein ◽  
Human Fibroblasts ◽  
Density Lipoprotein ◽  
Tryptic Digestion ◽  
Low Density ◽  
Lipoprotein Receptors

Occurrence of low density lipoprotein receptors within large pits on the surface of human fibroblasts as demonstrated by freeze-etching

1978 ◽  
Vol 113 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Lelio Orci ◽  
Jean-Louis Carpentier ◽  
A. Perrelet ◽  
Richard G.W. Anderson ◽  
Joseph L. Goldstein ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Human Fibroblasts ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptors ◽  
Freeze Etching
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