Correction: Rare Maternal mRNAs Code for Regulatory Proteins that Control Lineage-Specific Gene Expression in the Sea Urchin Embryo

1990 ◽  
Vol 87 (24) ◽  
pp. 10068b-10068
Author(s):  
A. E. Cutting
Keyword(s):  
Gene Expression ◽  
Sea Urchin ◽  
Regulatory Proteins ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Sea Urchin Embryo ◽  
Maternal Mrnas ◽  
Lineage Specific Gene

scholarly journals Lineage-specific Gene Expression in the Sea Urchin Embryo

1985 ◽  
Vol 50 (0) ◽  
pp. 321-328 ◽  
Author(s):  
E.H. Davidson ◽  
C.N. Flytzanis ◽  
J.J. Lee ◽  
J.J. Robinson ◽  
S.J. Rose ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sea Urchin ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Sea Urchin Embryo ◽  
Lineage Specific Gene

Primary differentiation and ectoderm-specific gene expression in the animalized sea urchin embryo

1985 ◽  
Vol 109 (2) ◽  
pp. 418-427 ◽  
Author(s):  
Martin Nemer ◽  
David G. Wilkinson ◽  
Elizabeth C. Travaglini
Keyword(s):  
Gene Expression ◽  
Sea Urchin ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Sea Urchin Embryo

scholarly journals Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium

2020 ◽  
Vol 48 (6) ◽  
pp. 2880-2896 ◽  
Author(s):  
Jun Li ◽  
Ting Zhang ◽  
Aarthi Ramakrishnan ◽  
Bernd Fritzsch ◽  
Jinshu Xu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Regulatory Elements ◽  
Sensory Epithelium ◽  
Hair Bundle ◽  
Specific Gene ◽  
Cell Type ◽  
Specific Gene Expression ◽  
Wide Range ◽  
Cell Type Specific ◽  
Lineage Specific Gene

Abstract The transcription factor Six1 is essential for induction of sensory cell fate and formation of auditory sensory epithelium, but how it activates gene expression programs to generate distinct cell-types remains unknown. Here, we perform genome-wide characterization of Six1 binding at different stages of auditory sensory epithelium development and find that Six1-binding to cis-regulatory elements changes dramatically at cell-state transitions. Intriguingly, Six1 pre-occupies enhancers of cell-type-specific regulators and effectors before their expression. We demonstrate in-vivo cell-type-specific activity of Six1-bound novel enhancers of Pbx1, Fgf8, Dusp6, Vangl2, the hair-cell master regulator Atoh1 and a cascade of Atoh1’s downstream factors, including Pou4f3 and Gfi1. A subset of Six1-bound sites carry consensus-sequences for its downstream factors, including Atoh1, Gfi1, Pou4f3, Gata3 and Pbx1, all of which physically interact with Six1. Motif analysis identifies RFX/X-box as one of the most significantly enriched motifs in Six1-bound sites, and we demonstrate that Six1-RFX proteins cooperatively regulate gene expression through binding to SIX:RFX-motifs. Six1 targets a wide range of hair-bundle regulators and late Six1 deletion disrupts hair-bundle polarity. This study provides a mechanistic understanding of how Six1 cooperates with distinct cofactors in feedforward loops to control lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium.

Characterization of hematopoietic lineage-specific gene expression by ES cell in vitro differentiation induction system

Blood ◽  
2000 ◽  
Vol 95 (3) ◽  
pp. 870-878 ◽  
Author(s):  
Takumi Era ◽  
Toshiaki Takagi ◽  
Tomomi Takahashi ◽  
Jean-Christophe Bories ◽  
Toru Nakano
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Experimental System ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
Differentiation Induction ◽  
Lineage Specific Gene ◽  
Megakaryocytic Cell

The continuous generation of mature blood cells from hematopoietic progenitor cells requires a highly complex series of molecular events. To examine lineage-specific gene expression during the differentiation process, we developed a novel method combiningLacZ reporter gene analysis with in vitro hematopoietic differentiation induction from mouse embryonic stem cells. For a model system using this method, we chose the erythroid and megakaryocytic differentiation pathways. Although erythroid and megakaryocytic cells possess distinct functional and morphologic features, these 2 lineages originate from bipotential erythro-megakaryocytic progenitors and share common lineage-restricted transcription factors. A portion of the 5′ flanking region of the human glycoprotein IIb (IIb) integrin gene extending from base −598 to base +33 was examined in detail. As reported previously, this region is sufficient for megakaryocyte-specific gene expression. However, previous reports that used human erythro-megakaryocytic cell lines suggested that one or more negative regulatory regions were necessary for megakaryocyte-specific gene expression. Our data clearly showed that an approximately 200-base enhancer region extending from −598 to −400 was sufficient for megakaryocyte-specific gene expression. This experimental system has advantages over those using erythro-megakaryocytic cell lines because it recapitulates normal hematopoietic cell development and differentiation. Furthermore, this system is more efficient than transgenic analysis and can easily examine gene expression with null mutations of specific genes.

HIV-1-derived self-inactivating lentivirus vector induces megakaryocyte lineage-specific gene expression

2005 ◽  
Vol 7 (2) ◽  
pp. 240-247 ◽  
Author(s):  
Kazuta Yasui ◽  
Rika A. Furuta ◽  
Kayoko Matsumoto ◽  
Yoshihiko Tani ◽  
Jun-ichi Fujisawa
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Lentivirus Vector ◽  
Specific Gene Expression ◽  
Lineage Specific Gene ◽  
Hiv 1
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