scholarly journals Regulation of Wnt5a mRNA Expression in Human Mammary Epithelial Cells by Cell Shape, Confluence, and Hepatocyte Growth Factor

1995 ◽  
Vol 270 (21) ◽  
pp. 12851-12856 ◽  
Author(s):  
Emmanuel L. Huguet ◽  
Kenneth Smith ◽  
Roy Bicknell ◽  
Adrian L. Harris
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Mrna Expression ◽  
Cell Shape ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial ◽  
Hepatocyte Growth

Branching morphogenesis of human mammary epithelial cells in collagen gels

1994 ◽  
Vol 107 (12) ◽  
pp. 3557-3568 ◽  
Author(s):  
F. Berdichevsky ◽  
D. Alford ◽  
B. D'Souza ◽  
J. Taylor-Papadimitriou
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Mammary Epithelial Cells ◽  
Branching Morphogenesis ◽  
Mammary Epithelial ◽  
Collagen Gels ◽  
Scatter Factor ◽  
Human Mammary Epithelial ◽  
Hepatocyte Growth

To study the morphogenesis of human epithelial cells in vitro we have used a three-dimensional collagen matrix and a newly developed mammary epithelial cell line, 1–7 HB2. In standard medium 1–7 HB2 cells formed compact balls/spheres inside collagen type I gels, while cocultivation with various fibroblast cell lines or growth in fibroblast-conditioned media resulted in the appearance of branching structures. At least two different soluble factors secreted by fibroblasts were found to be implicated in the branching morphogenesis. Firstly, hepatocyte growth factor/scatter factor could induce branching in a concentration-dependent manner. Moreover, a polyclonal serum against hepatocyte growth factor/scatter factor completely inhibited the branching morphogenesis induced by medium conditioned by MRC-5 fibroblast cells. In contrast, a morphogenetic activity secreted by human foreskin fibroblasts was identified that appears to be different from hepatocyte growth factor/scatter factor and from a number of other well-characterized growth factors or cytokines. This model system has been used to examine the role of integrins in mammary morphogenesis. The expression of the alpha 2 beta 1, alpha 3 beta 1 and alpha 6 beta 4 integrins was decreased when cells were plated on collagen gels. The addition of specific blocking monoclonal antibodies directed to the alpha 2- and beta 1-integrin subunits to growth media impaired cell-cell interactions and interfered with the formation of compact structures inside collagen gels, suggesting that the alpha 2 beta 1 integrin can control intercellular adhesion in mammary morphogenesis. In contrast one of the blocking monoclonal antibodies against the alpha 3-integrin subunit (P1B5) mimicked the effect of soluble ‘morphogens’. Our results suggest that the modulation of alpha 3 beta 1 activity may represent an important event in the induction of branching morphogenesis of human mammary epithelial cells.

Actions of the soy phytoestrogen genistein in models of human chronic disease: potential involvement of transforming growth factor β

2001 ◽  
Vol 29 (2) ◽  
pp. 216-222 ◽  
Author(s):  
H. Kim ◽  
J. Xu ◽  
Y. Su ◽  
H. Xia ◽  
L. Li ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Transforming Growth Factor ◽  
Mammary Epithelial Cells ◽  
Hormone Replacement ◽  
Transforming Growth Factor Β ◽  
Mammary Epithelial ◽  
Primate Model ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial

The structural similarity, but non-identity, between 17β-oestradiol and the soy phytoestrogen genistein suggests that the two compounds will have actions that may be identical in some target biological systems, but different in others. Epidermal growth factor (EGF)-stimulated proliferation of human mammary epithelial cells (that do not express the oestrogen receptor) was significantly suppressed at genistein concentrations (5–10μM) that are attainable physiologically. Others have shown previously that transforming growth factor β (TGFβ) has similar growth-inhibitory effects on human cells. Analysis of the conditioned medium of human mammary epithelial cells exposed to genistein plus EGF showed increased levels of TGFβ relative to those in the medium of cells exposed to EGF or genistein alone. Related experiments in a primate model of menopause demonstrated that ingestion of soy containing isoflavones was correlated with the suppression of neurodegeneration-relevant phosphorylation of the microtubule-associated protein tau, while intake of Premarin (a hormone replacement therapy that is commonly prescribed for women) was not correlated. The results discussed here indicate that genistein, and probably other related phytoestrogens, have pleiotropic actions, some of which may involve TGFβ activity.

scholarly journals erbB-2 Overexpression in Human Mammary Epithelial Cells Confers Growth Factor Independence*

Endocrinology ◽  
1999 ◽  
Vol 140 (8) ◽  
pp. 3615-3622 ◽  
Author(s):  
Kathleen M. Woods Ignatoski ◽  
Allison J. LaPointe ◽  
Eric H. Radany ◽  
Stephen P. Ethier
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial
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