scholarly journals NdhP Is an Exclusive Subunit of Large Complex of NADPH Dehydrogenase Essential to Stabilize the Complex inSynechocystissp. Strain PCC 6803

2014 ◽  
Vol 289 (27) ◽  
pp. 18770-18781 ◽  
Author(s):  
Jingsong Zhang ◽  
Fudan Gao ◽  
Jiaohong Zhao ◽  
Teruo Ogawa ◽  
Quanxi Wang ◽  
...  
Keyword(s):  
Large Complex ◽  
Nadph Dehydrogenase ◽  
Pcc 6803

scholarly journals Subunit Q Is Required to Stabilize the Large Complex of NADPH Dehydrogenase in Synechocystis sp. Strain PCC 6803

2015 ◽  
Vol 168 (2) ◽  
pp. 443-451 ◽  
Author(s):  
Jiaohong Zhao ◽  
Weiqiong Rong ◽  
Fudan Gao ◽  
Teruo Ogawa ◽  
Weimin Ma
Keyword(s):  
Large Complex ◽  
Nadph Dehydrogenase ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Sustained Photoevolution of Molecular Hydrogen in a Mutant of Synechocystis sp. Strain PCC 6803 Deficient in the Type I NADPH-Dehydrogenase Complex

2004 ◽  
Vol 186 (6) ◽  
pp. 1737-1746 ◽  
Author(s):  
Laurent Cournac ◽  
Geneviève Guedeney ◽  
Gilles Peltier ◽  
Paulette M. Vignais
Keyword(s):  
Photosynthetic Electron Transport ◽  
Type I ◽  
Adaptation Period ◽  
Hydrogen Metabolism ◽  
Whole Cells ◽  
Time Period ◽  
Nadph Dehydrogenase ◽  
Dehydrogenase Complex ◽  
Pcc 6803

ABSTRACT The interaction between hydrogen metabolism, respiration, and photosynthesis was studied in vivo in whole cells of Synechocystis sp. strain PCC 6803 by continuously monitoring the changes in gas concentrations (H2, CO2, and O2) with an online mass spectrometer. The in vivo activity of the bidirectional [NiFe]hydrogenase [H2:NAD(P) oxidoreductase], encoded by the hoxEFUYH genes, was also measured independently by the proton-deuterium (H-D) exchange reaction in the presence of D2. This technique allowed us to demonstrate that the hydrogenase was insensitive to light, was reversibly inactivated by O2, and could be quickly reactivated by NADH or NADPH (+H2). H2 was evolved by cells incubated anaerobically in the dark, after an adaptation period. This dark H2 evolution was enhanced by exogenously added glucose and resulted from the oxidation of NAD(P)H produced by fermentation reactions. Upon illumination, a short (less than 30-s) burst of H2 output was observed, followed by rapid H2 uptake and a concomitant decrease in CO2 concentration in the cyanobacterial cell suspension. Uptake of both H2 and CO2 was linked to photosynthetic electron transport in the thylakoids. In the ndhB mutant M55, which is defective in the type I NADPH-dehydrogenase complex (NDH-1) and produces only low amounts of O2 in the light, H2 uptake was negligible during dark-to-light transitions, allowing several minutes of continuous H2 production. A sustained rate of photoevolution of H2 corresponding to 6 μmol of H2 mg of chlorophyll−1 h−1 or 2 ml of H2 liter−1 h−1 was observed over a longer time period in the presence of glucose and was slightly enhanced by the addition of the O2 scavenger glucose oxidase. By the use of the inhibitors DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea] and DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone), it was shown that two pathways of electron supply for H2 production operate in M55, namely photolysis of water at the level of photosystem II and carbohydrate-mediated reduction of the plastoquinone pool.

scholarly journals NdhO, a Subunit of NADPH Dehydrogenase, Destabilizes Medium Size Complex of the Enzyme inSynechocystissp. Strain PCC 6803

2014 ◽  
Vol 289 (39) ◽  
pp. 26669-26676 ◽  
Author(s):  
Jiaohong Zhao ◽  
Fudan Gao ◽  
Jingsong Zhang ◽  
Teruo Ogawa ◽  
Weimin Ma
Keyword(s):  
Medium Size ◽  
A Subunit ◽  
Nadph Dehydrogenase ◽  
Pcc 6803

scholarly journals NdhV Is a Subunit of NADPH Dehydrogenase Essential for Cyclic Electron Transport in Synechocystis sp. Strain PCC 6803

2015 ◽  
Vol 170 (2) ◽  
pp. 752-760 ◽  
Author(s):  
Fudan Gao ◽  
Jiaohong Zhao ◽  
Xiaozhuo Wang ◽  
Shen Qin ◽  
Lanzhen Wei ◽  
...  
Keyword(s):  
Electron Transport ◽  
Cyclic Electron Transport ◽  
A Subunit ◽  
Nadph Dehydrogenase ◽  
Synechocystis Sp ◽  
Pcc 6803

Using the principles and approaches of flexible methodology (agile) in the planning and implementation of the educational process

2020 ◽  
pp. 48-55
Author(s):  
TING ZHU
Keyword(s):  
Labor Market ◽  
Program Development ◽  
Development Process ◽  
Project Manager ◽  
Educational Process ◽  
Large Complex ◽  
Complex Projects ◽  
Quality Risk ◽  
The Government

The educatonal program development process is challenging with many stakeholders: students, teachers, parents, administrators, employers, and the government. Each stakeholder has compettve priorites; Quality, risk, resources and costs limitaton. Large complex projects require the management of a skilled and experienced project manager. It is the preparaton of curricula for joint internatonal educatonal programs with the use of Agile tools and technologies. The main "customer" in this program is a student, and the ultmate goal of each project within the program-the competence of the student in the labor market afer receiving the diploma. Therefore, this program can be considered difcult, and it is constantly evolves, because the demands of the labor market of China is constantly changing and developing.

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