Enhancement of rat tear IgA antibody responses following intranasal immunization with antigen and CpG ODN

Successful vaccines against specific pathogens often require multiple immunizations and adjuvant usage. Yet, assessing the protective efficacy of different immunization regimens with adjuvanted Toxoplasma gondii vaccines remains elusive. In this study, we investigated the vaccine efficacy induced by CpG-ODN-adjuvanted T. gondii virus-like particles (VLPs) after challenge infection with T. gondii (ME49) in mice (BALB/c) upon one, two, and three immunizations. Immunization with adjuvanted T. gondii VLPs induced higher levels of T. gondii-specific IgG and/or IgA antibody responses, germinal center (GC) B cells, total B cells, and CD4+ and CD8+ T cells compared with unadjuvanted VLPs. Increasing the number of immunizations was strongly correlated with enhanced protective immunity against T. gondii in mice, with the highest protection being demonstrated in mice thrice-immunized with either adjuvanted T. gondii VLPs or VLPs alone. Notably, lesser bodyweight reductions and cerebral cyst counts were observed in mice receiving multiple immunizations with the adjuvanted VLPs, thereby confirming the effectiveness of adjuvanted boost immunizations. These results demonstrated that multiple immunizations with T. gondii VLPs is an effective approach, and the CpG-ODN can be developed as an effective adjuvant for T. gondii VLP vaccines.

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IgG, IgM and IgA antibody responses against immediate-early, early and late human cytomegalovirus proteins in patients with HCMV infections

Serodiagnosis and Immunotherapy in Infectious Disease ◽

10.1016/0888-0786(95)97895-c ◽

1995 ◽

Vol 7 (3) ◽

pp. 121-128

Author(s):

M. Segondy ◽

S.L Salhi

Keyword(s):

Human Cytomegalovirus ◽

Antibody Responses ◽

Immediate Early ◽

Iga Antibody

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Similar subclass antibody responses after intranasal immunization with UV-inactivated RSV mixed with cholera toxin or live RSV

Journal of Medical Virology ◽

10.1002/jmv.1890350309 ◽

1991 ◽

Vol 35 (3) ◽

pp. 192-197 ◽

Cited By ~ 18

Author(s):

Peter D. Reuman ◽

Scott P. Keely ◽

Gilbert M. Schiff ◽

James N. Gamble

Keyword(s):

Cholera Toxin ◽

Antibody Responses ◽

Intranasal Immunization

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Age-Specific Immunoglobulin G (IgG) and IgA to Pneumococcal Protein Antigens in a Population in Coastal Kenya

Infection and Immunity ◽

10.1128/iai.72.6.3331-3335.2004 ◽

2004 ◽

Vol 72 (6) ◽

pp. 3331-3335 ◽

Cited By ~ 30

Author(s):

Catherine Laine ◽

Tabitha Mwangi ◽

Claudette M. Thompson ◽

Jacktone Obiero ◽

Marc Lipsitch ◽

...

Keyword(s):

Immunoglobulin G ◽

Protein A ◽

Age Groups ◽

Surface Protein ◽

The Elderly ◽

Antibody Responses ◽

Sub Saharan Africa ◽

Protein Antigens ◽

Coastal Kenya ◽

Iga Antibody

ABSTRACT Streptococcus pneumoniae is the primary etiological agent of community-acquired pneumonia and a major cause of meningitis and bacteremia. Three conserved pneumococcal proteins—pneumolysin, pneumococcal surface adhesin A (PsaA), and pneumococcal surface protein A (PspA)—are currently being investigated as vaccine candidates. Such protein-based vaccines, if proven effective, could provide a cheaper alternative to conjugate vaccine formulae. Few data from sub-Saharan Africa exist concerning the development of natural antibody to these antigens, however. To investigate the age-specific development of antiprotein immunoglobulin G (IgG) and IgA antibody responses, the sera of 220 persons 2 weeks to 84 years of age from coastal Kenya were assayed using enzyme-linked immunosorbent assays. IgG and IgA antibody responses to each antigen were observed in all age groups. Serum concentrations of IgG and IgA antibody responses to PspA and PdB (a recombinant toxoid derivative of pneumolysin), but not to PsaA, increased significantly with age (P < 0.001). No decline was observed in the sera of the elderly. Anti-protein IgG concentrations were only weakly correlated (0.30 < r < 0.56; P < 0.0001), as were IgA concentrations (0.24 < r < 0.54; P < 0.0001).

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Kinetics of antigen-specific IgM/IgG/IgA antibody responses during Zika virus natural infection in two patients

Journal of Medical Virology ◽

10.1002/jmv.25366 ◽

2018 ◽

Vol 91 (5) ◽

pp. 872-876 ◽

Cited By ~ 3

Author(s):

Ling-Zhai Zhao ◽

Wen-Xin Hong ◽

Jian Wang ◽

Lei Yu ◽

Feng-Yu Hu ◽

...

Keyword(s):

Zika Virus ◽

Natural Infection ◽

Antibody Responses ◽

Iga Antibody ◽

Kinetics Of

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Immunoprofiles associated with controlled human malaria infection and naturally acquired immunity identify a shared IgA pre-erythrocytic immunoproteome

npj Vaccines ◽

10.1038/s41541-021-00363-y ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Andrea A. Berry ◽

Joshua M. Obiero ◽

Mark A. Travassos ◽

Amed Ouattara ◽

Drissa Coulibaly ◽

...

Keyword(s):

Malaria Infection ◽

Vaccine Development ◽

Protein Microarray ◽

Antibody Responses ◽

Liver Stage ◽

Human Malaria ◽

Controlled Human Malaria Infection ◽

Iga Antibody ◽

Potential Vaccine ◽

Antigen Protein

AbstractKnowledge of the Plasmodium falciparum antigens that comprise the human liver stage immunoproteome is important for pre-erythrocytic vaccine development, but, compared with the erythrocytic stage immunoproteome, more challenging to classify. Previous studies of P. falciparum antibody responses report IgG and rarely IgA responses. We assessed IgG and IgA antibody responses in adult sera collected during two controlled human malaria infection (CHMI) studies in malaria-naïve volunteers and in 1- to 6-year-old malaria-exposed Malian children on a 251 P. falciparum antigen protein microarray. IgG profiles in the two CHMI groups were equivalent and differed from Malian children. IgA profiles were robust in the CHMI groups and a subset of Malian children. We describe immunoproteome differences in naïve vs. exposed individuals and report pre-erythrocytic proteins recognized by the immune system. IgA responses detected in this study expand the list of pre-erythrocytic antigens for further characterization as potential vaccine candidates.

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Immunoglobulin A Antibody Responses in Dengue Patients: a Useful Marker for Serodiagnosis of Dengue Virus Infection

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.12.10.1235-1237.2005 ◽

2005 ◽

Vol 12 (10) ◽

pp. 1235-1237 ◽

Cited By ~ 14

Author(s):

M. Nawa ◽

T. Takasaki ◽

M. Ito ◽

S. Inoue ◽

K. Morita ◽

...

Keyword(s):

Virus Infection ◽

Dengue Virus ◽

Immunoglobulin A ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Virus Infections ◽

Serum Samples ◽

Dengue Virus Infection ◽

Iga Antibody ◽

Antibody Capture

ABSTRACT We determined the usefulness of an immunoglobulin A (IgA) antibody-capture enzyme-linked immunosorbent assay for serodiagnosis of dengue virus infections. The results indicate that the presence of IgA and IgM in serum samples assures recent primary dengue virus infection even with a single serum sample.

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Usefulness of the IgA and IgG Responses to Macrophage Migration Inhibitory Factor for the Diagnosis of Tuberculosis

Diagnostics ◽

10.3390/diagnostics10110991 ◽

2020 ◽

Vol 10 (11) ◽

pp. 991

Author(s):

Ji Yeon Lee ◽

Byoung-Jun Kim ◽

Jee-min Kim ◽

Junghyun Kim ◽

Joon-Sung Joh ◽

...

Keyword(s):

Migration Inhibitory Factor ◽

Macrophage Migration Inhibitory Factor ◽

Latent Tuberculosis ◽

Protective Role ◽

Antibody Responses ◽

Inhibitory Factor ◽

Macrophage Migration ◽

Serological Tests ◽

Iga Antibody ◽

Ifn Γ

Serological tests offer the potential in order to improve the diagnosis of tuberculosis (TB). Macrophage migration inhibitory factor (MIF) plays a protective role in infection control in TB; however, to date, no studies on antibody responses to MIF have been reported. We measured immunoglobulin (Ig)A and IgG responses to MIF in individuals with either active tuberculosis (ATB; n = 65), latent tuberculosis (LTBI; n = 53), or in non-infected individuals (NI; n = 62). The QuantiFERON-TB Gold In-Tube (QFT-GIT) assay was used in order to screen for LTBI. The level of IgA against MIF was significantly lower in LTBI and ATB patients than in NI individuals, was significantly related to LTBI and ATB diagnosis, and it could discriminate between LTBI and ATB. In contrast, the level of IgG against MIF was significantly lower in LTBI patients than in NI individuals and was significantly related to LTBI diagnosis. Anti-MIF IgG levels were significantly lower in AFB-negative TB, minimal TB, and new ATB patients, than in the NI group. IgA and IgG levels against MIF both showed significant negative correlations with IFN-γ levels, as assessed using the QFT-GIT test. Although none of the antibodies could achieve high diagnostic predictive power individually, our results suggest the possibility of using IgA antibody responses to MIF in the diagnosis of LTBI and ATB.

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