Efficacy of entomopathogenic nematodes against lepidopteran insect pests.

2017 ◽  
pp. 157-173 ◽  
Author(s):  
M. M. E. Saleh
Keyword(s):  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Lepidopteran Insect

scholarly journals Effect of mini-sprinkler irrigation system on Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae) infective juvenile

2008 ◽  
Vol 65 (4) ◽  
pp. 433-437 ◽  
Author(s):  
Juan Carlos Lara ◽  
Cláudia Dolinski ◽  
Elias Fernandes de Sousa ◽  
Rogério Figueiredo Daher
Keyword(s):  
Entomopathogenic Nematodes ◽  
Galleria Mellonella ◽  
Insect Pests ◽  
Irrigation System ◽  
Larval Mortality ◽  
Sprinkler Irrigation ◽  
Infective Juvenile ◽  
Application Techniques ◽  
Temperature And Pressure ◽  
Search Capability

Entomopathogenic nematodes (EPNs) are currently being used as successful biological control agents of soil-dwelling insect pests. Previous field and greenhouse studies demonstrated that application techniques and non-biotic factors (temperature and pressure) have a significant effect on EPNs efficacy. The objective of this study was to evaluate the influence of an irrigation spray application system on the viability, infectivity and host search capability of Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae) infective juveniles (IJ). Two assays were proposed. Their viability was evaluated under the microscope after the IJ passed through the irrigation system. Infectivity on Galleria mellonella larvae, and host search capability, as evidenced by larval mortality, were evaluated in containers (Experiment 1). In the field (Experiment 2), mortality of G. mellonella larvae was evaluated under different nematode concentrations (0, 100,000, 300,000 and 500,000 IJ per tree). No differences were recorded on the viability, infectivity and host search capability of the IJ in Experiment 1. In Experiment 2, differences were recorded among the different concentrations used (p < 0.05), and a higher mortality was observed at the highest nematode concentration (28.3% and 37% in each one of the two experiment repetitions). This irrigation system did not affected adversely the viability, infectivity and host search capability of H. baujardi LPP7.

scholarly journals UJI EFEKTIVITAS NEMATODA ENTOMOPATOGEN (RHABDITIDA: Steinernema DAN Heterorhabditis) SEBAGAI MUSUH ALAMI NON-ENDEMIK PENGGEREK BATANG PADI KUNING (Scirpophaga incertulas)

2007 ◽  
Vol 7 (2) ◽  
pp. 71-79
Author(s):  
Chaerani Chaerani ◽  
Bebet Nurbaeti
Keyword(s):  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Larval Mortality ◽  
Damage Threshold ◽  
Stem Borer ◽  
Leaf Sheath ◽  
Scirpophaga Incertulas ◽  
Potted Plants ◽  
Plant Damage ◽  
Rice Stem Borer

Efficacy Tests of Entomopathogenic Nematodes (Rhabditida:  Steinernema dan Heterorhabditis) as Non-endemic Natural Enemies of Yellow Rice stem Borer (Scirpophaga incertulas). Yellow rice stem borer (Scirpophaga incertulas) is a chronic insect pests of irrigated rice and difficult to control.  Entomopathogenic nematodes from the genus Steinernema and Heterorhabditis are promising biological control agents for this pest as their infective juveniles (IJs) are capable of seeking and infecting insect living in moist, cryptic habitat such as galleries created by stem borer larvae. Thirteen indigenous and exotic Steinernema and Heterorhabditis sprayed to rice seedlings in laboratory with nematodes at concentrations of 0.5 or 2.0×104 IJs ml-1 water caused larval mortality between 7–93%.  Further test in greenhouse on nematodes that had >50%  efficacy showed that an indigenous isolate, H. indicus INA H17, was the most effective among the tested nematodes in killing larvae or pupae (78%).  Reduction in plant damage caused by the insects could not be demonstrated as the trials was limited to potted plants. The survival ability of nematodes on rice plants was evaluated by using INA H4 as an example. A low percentage of INA H4 IJs (0.5%) persisted in inner leaf sheath until 7 days post application, while IJs on leaf surface and outer leaf sheath survived only until 2 and 48 hours post application, respectively. Improvement of application strategies including repeated spray, addition of antidessicant and adjusment of spray volume and application at damage threshold or plant critical period are deemed necessary to enhance nematode efficacy and reduce plant damage in the field.

scholarly journals Entomopathogenic nematodes: can we use the current knowledge on belowground multitrophic interactions in future plant protection programmes? – Review

2019 ◽  
Vol 55 (No. 4) ◽  
pp. 242-253 ◽  
Author(s):  
Anamarija Jagodič ◽  
Stanislav Trdan ◽  
Žiga Laznik
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Current Knowledge ◽  
Plant Protection ◽  
Multitrophic Interactions ◽  
Herbivore Attack ◽  
Below Ground ◽  
Chemical Stimuli

Plants under herbivore attack emit mixtures of volatiles that can attract the natural enemies of the herbivores. Entomopathogenic nematodes (EPNs) are organisms that can be used in the biological control of insect pests. Recent studies have shown that the movement of EPNs is associated with the detection of chemical stimuli from the environment. To date, several compounds that are responsible for the mediation in below ground multitrophic interactions have been identified. In the review, we discuss the use of EPNs in agriculture, the role of belowground volatiles and their use in plant protection programmes.

A survey of entomopathogenic nematodes and their symbiotic bacteria in agricultural areas of northern Thailand

2020 ◽  
Vol 94 ◽  
Author(s):  
J. Ardpairin ◽  
P. Muangpat ◽  
S. Sonpom ◽  
A. Dumidae ◽  
C. Subkrasae ◽  
...  
Keyword(s):  
Entomopathogenic Nematodes ◽  
Biocontrol Agent ◽  
Insect Pests ◽  
Soil Samples ◽  
Symbiotic Bacteria ◽  
Soil Parameters ◽  
Field Crops ◽  
Indian Strain ◽  
Agricultural Areas ◽  
28S Ribosomal Dna

Abstract Entomopathogenic nematodes (EPNs) Steinernema and Heterorhabditis and their symbiotic bacteria, Xenorhabdus and Photorhabdus, have been successfully used for the control of insect pests. The objectives of this study were to survey the EPNs and symbiotic bacteria in the agricultural areas of the Phitsanulok province, Thailand, and to study the association between the soil parameters and presence of EPNs. We collected 200 soil samples from 40 soil sites in agricultural areas (field crops, horticulture crops and forest). The prevalence of EPNs was 8.0% (16/200). Fifteen of the EPN isolates were molecularly identified (based on 28S ribosomal DNA and internal transcribed spacer regions) as Steinernema siamkayai. Seven isolates of Xenorhabdus stockiae were identified using recombinase A sequencing. Phylogenetic analysis revealed that all the Steinernema and Xenorhabdus isolates were closely related to S. siamkayai (Indian strain) and X. stockiae (Thai strain), respectively. Significantly more EPNs were recovered from loam than from clay. Although the association between soil parameters (pH, temperature and moisture) and the presence of EPNs was not statistically significant, the elevation levels of the soil sites with and without EPNs were found to be different. Moreover, statistical comparisons between the agricultural areas revealed no significant differences. Therefore, we concluded that S. siamkayai is associated with X. stockiae in agricultural areas and that there is no association between the soil parameters of agricultural areas and presence of EPNs, except for soil texture and the elevation. Steinernema siamkayai may be applied as a biocontrol agent in agricultural areas.

scholarly journals Bacteria from the Midgut of Common Cockchafer (Melolontha melolontha L.) Larvae Exhibiting Antagonistic Activity Against Bacterial Symbionts of Entomopathogenic Nematodes: Isolation and Molecular Identification

2020 ◽  
Vol 21 (2) ◽  
pp. 580 ◽  
Author(s):  
Marcin Skowronek ◽  
Ewa Sajnaga ◽  
Małgorzata Pleszczyńska ◽  
Waldemar Kazimierczak ◽  
Magdalena Lis ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Acinetobacter Calcoaceticus ◽  
Inhibitory Effect ◽  
Antagonistic Activity ◽  
Reca Gene ◽  
Melolontha Melolontha ◽  
Insect Gut

The mechanisms of action of the complex including entomopathogenic nematodes of the genera Steinernema and Heterorhabditis and their mutualistic partners, i.e., bacteria Xenorhabdus and Photorhabdus, have been well explained, and the nematodes have been commercialized as biological control agents against many soil insect pests. However, little is known regarding the nature of the relationships between these bacteria and the gut microbiota of infected insects. In the present study, 900 bacterial isolates that were obtained from the midgut samples of Melolontha melolontha larvae were screened for their antagonistic activity against the selected species of the genera Xenorhabdus and Photorhabdus. Twelve strains exhibited significant antibacterial activity in the applied tests. They were identified based on 16S rRNA and rpoB, rpoD, or recA gene sequences as Pseudomonas chlororaphis, Citrobacter murliniae, Acinetobacter calcoaceticus, Chryseobacterium lathyri, Chryseobacterium sp., Serratia liquefaciens, and Serratia sp. The culture filtrate of the isolate P. chlororaphis MMC3 L3 04 exerted the strongest inhibitory effect on the tested bacteria. The results of the preliminary study that are presented here, which focused on interactions between the insect gut microbiota and mutualistic bacteria of entomopathogenic nematodes, show that bacteria inhabiting the gut of insects might play a key role in insect resistance to entomopathogenic nematode pressure.

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