scholarly journals Effect of dietary phospholipid level and phospholipid:neutral lipid value on the development of sea bass (Dicentrarchus labrax) larvae fed a compound diet

2003 ◽  
Vol 90 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Chantal L. Cahu ◽  
José L. Zambonino Infante ◽  
Valérie Barbosa
Keyword(s):  
Phospholipase A2 ◽  
Pancreatic Lipase ◽  
Fish Larvae ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Phospholipid Content ◽  
Transcriptional Level ◽  
Good Growth ◽  
Live Prey ◽  
Larval Quality

The aim of the study was to determine the influence of dietary phospholipid concentration on survival and development in sea bass (Dicentrarchus labrax) larvae. Larvae were fed from day 9 to day 40 post-hatch with an isoproteic and isolipidic formulated diet with graded phospholipid levels from 27 to 116 g/kg DM and different phospholipid:neutral lipid values. The best growth (32 mg at the end of the experiment) survival (73 %) and larval quality (only 2% of malformed larvae) were obtained in the larvae fed the diet containing 116 g phospholipid/kg DM (P < 0·05). These results were related to the amount of phosphatidylcholine and phosphatidylinositol included in this diet (35 and 16 g/kg respectively). Amylase, alkaline phosphatase and aminopeptidase N activities revealed a proper maturation of the digestive tract in the two groups fed the highest phospholipid levels. Regulation of lipase and phospholipase A2 by the relative amount of their substrate in the diet occurred mainly at the transcriptional level. The response of pancreatic lipase to dietary neutral lipid was not linear. As in mammals 200 g triacylglycerol/kg diet seems to represent a threshold level above which the response of pancreatic lipase is maximal. The response of phospholipase A2 to dietary phospholipid content was gradual and showed a great modulation range in expression. Sea bass larvae have more efficient capacity to utilize dietary phospholipid than neutral lipids. For the first time a compound diet sustaining good growth, survival and skeletal development has been formulated and can be used in total replacement of live prey in the feeding sequence of marine fish larvae.

Quantification of muscle condition using digital image analysis in Dicentrarchus labrax larvae, and relationship with survival

2002 ◽  
Vol 82 (4) ◽  
pp. 649-654 ◽  
Author(s):  
Ignacio A. Catalán ◽  
M. Pilar Olivar
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Fish Larvae ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Critical Value ◽  
Nutritional Condition ◽  
Dicentrarchus Labrax Larvae

Digital image analysis was applied to quantify the degradation of muscle in sea bass Dicentrarchus labrax (Pisces: Moronidae) larvae. The measure obtained was the percentage muscle fibre separation (MFS). This measurement was compared to that obtained through classical scoring. The percentage MFS classified correctly a larger number of larvae from different feeding treatments (Fed, Starved and Delayed treatments) and paralleled nutritional condition, both by age and by size. It also yielded a significant correlation with the survival of the Starved larvae. The onset of the mass mortality of Starved larvae was used to define a critical value (Mmax) of 6% MFS which would severely handicap survival. We regard MFS as a useful contribution to the determination of condition in fish larvae. It is a relatively fast quantitative method, and its use would reduce the bias caused by discrete grading (scores) and by differences in individual expertise.

scholarly journals Is the perceived resiliency of fish larvae to ocean acidification masking more subtle effects?

2013 ◽  
Vol 10 (10) ◽  
pp. 17043-17070 ◽  
Author(s):  
E. C. Pope ◽  
R. P. Ellis ◽  
M. Scolamacchia ◽  
J. W. S. Scolding ◽  
A. Keay ◽  
...  
Keyword(s):  
Ocean Acidification ◽  
Early Life ◽  
Fish Larvae ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Life Stages ◽  
Daily Mortality ◽  
Early Life Stages ◽  
European Sea Bass ◽  
Near Future

Abstract. Ocean acidification, caused by rising concentrations of carbon dioxide (CO2), is widely considered to be a major global threat to marine ecosystems. To investigate the potential effects of ocean acidification on the early life stages of a commercially important fish species, European sea bass (Dicentrarchus labrax), 12 000 larvae were incubated from hatch through metamorphosis under a matrix of two temperatures (17 and 19 °C) and two seawater pCO2s (400 and 750 μatm) and sampled regularly for 42 days. Calculated daily mortality was significantly affected by both temperature and pCO2, with both increased temperature and elevated pCO2 associated with lower daily mortality and a significant interaction between these two factors. There was no significant pCO2 effect noted on larval morphology during this period but larvae raised at 19 °C possessed significantly larger eyes and lower carbon:nitrogen ratios at the end of the study compared to those raised under 17 °C. These results suggest that D. labrax larvae are resilient to near-future oceanic conditions. However, when the incubation was continued to post-metamorphic (juvenile) animals (day 67–69), fish raised under a combination of 19 °C and 750 μatm pCO2 were significantly heavier and exhibited lower aerobic scopes than those incubated at 19 °C and 400 μatm. Most other studies investigating the effects of near-future oceanic conditions on the early life stages of marine fish have used incubations of relatively short durations and suggested these animals are resilient to ocean acidification. We propose the durations of these other studies may be insufficient for more subtle effects, such as those observed in this study, to become apparent. These findings may have important implications for both sea bass in a changing ocean and also for the interpretation of results from other studies that have shown resiliency in marine teleosts exposed to higher atmospheric concentrations of CO2.

Influence of nutritional composition of diet on sea bass, Dicentrarchus labrax L., reproductive performance and egg and larval quality

Aquaculture ◽  
1994 ◽  
Vol 128 (3-4) ◽  
pp. 345-361 ◽  
Author(s):  
Joan Cerdá ◽  
Manuel Carrillo ◽  
Silvia Zanuy ◽  
Jesús Ramos ◽  
Manuel de la Higuera
Keyword(s):  
Reproductive Performance ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Nutritional Composition ◽  
Larval Quality

scholarly journals Intake of high levels of vitamin A and polyunsaturated fatty acids during different developmental periods modifies the expression of morphogenesis genes in European sea bass (Dicentrarchus labrax)

2006 ◽  
Vol 95 (4) ◽  
pp. 677-687 ◽  
Author(s):  
Laure A. N. Villeneuve ◽  
Enric Gisbert ◽  
Jacques Moriceau ◽  
Chantal L. Cahu ◽  
José L. Zambonino Infante
Keyword(s):  
Fatty Acids ◽  
Vitamin A ◽  
Fish Larvae ◽  
Control Diet ◽  
Juvenile Fish ◽  
Dicentrarchus Labrax ◽  
Subsequent Development ◽  
Sea Bass ◽  
Hypervitaminosis A ◽  
European Sea Bass

The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13d post-hatching (dph); period B: from 13 to 18dph; period C: from 18 to 23dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades, resulting in two different kinds of malformation. Hypervitaminosis A delayed development, reducing the number of vertebral segments and disturbing bone formation in the cephalic region. These malformations were correlated to an upregulation of retinoic acid receptor γ, retinoid X receptor (RXR) α and bone morphogenetic protein (BMP)4. An excess of PUFA accelerated the osteoblast differentiation process through the upregulation of RXRα and BMP4, leading to a supernumerary vertebra. These results suggest that the composition of diets devoted to marine fish larvae has a particularly determining effect before 13dph on the subsequent development of larvae and juvenile fish.

Studies on Prevailing Parasitic Fish Diseases in Pre-mature Cultured Sea bass, Dicentrarchus labrax, Sea bream and Mugil cephalus at Ismailia, Province with Special References to Control

2020 ◽  
Keyword(s):  
Parasitic Infection ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Mugil Cephalus ◽  
Sea Bream ◽  
Fish Diseases ◽  
Total Prevalence ◽  
Dark Colour ◽  
Gill Filaments ◽  
Mature Fish

This study concentrated on the assessment of the prevailing parasitic fish diseases in some marine fishes at Ismailia province and how to control the infestation using microalgae. This study was carried out on 1080 pre-mature fish (360 D. labrax (225±25 g) and 360 S aurata (150±25 g) and 360 M. cephalus (125±25 g) collected from similar ponds of studies to be examined at the end of treatment. In addition to that we followed non-treated fish (1080 premature). The infested fish showed dark colour and respiratory signs. Post mortem lesions were a presence of congestion or paleness and destruction of gill filaments. The total prevalence of infestation was the total prevalence of parasitic infection of non-treated fishes was 45.83 %. The highest percentage was in D. labrax 56.94 % followed by S. aurata 47.22%, the lowest percentage in M. cephalus 33.33. The total prevalence of parasitic infection in premature treated with 2 g algae was 28.79%, followed by 3 g algae was 23.60 %, while the lowest percentage with 5 g algae was 20.37 % respectively. The detected species of parasites were protozoal parasites, Amyloodinium ocellatum and Riboscyphidia in additions of marine monogenea, Lamellodiscus diplodicus isolated from D Labrex, Mugil Cephalus and S aurata. The present study concluded that, the use of microalgae instead of fish meal decreased parasitic infestation in marine fish. The histopathological alteration of natural infested examined fishes was also recorded.

Vaccination of European sea bass Dicentrarchus labrax with avirulent Mycobacterium marinum (iipA::kan mutant)

2019 ◽  
Vol 90 ◽  
pp. 317-327 ◽  
Author(s):  
Shay Ravid-Peretz ◽  
Angelo Colorni ◽  
Galit Sharon ◽  
Michal Ucko
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Mycobacterium Marinum ◽  
European Sea Bass
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